pFLDalpha

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联系方式：I47-825O-882O

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pFLDalpha载体 pFLD α质粒基本信息

出品公司:	Invitrigen
载体名称:	pFLDalpha、pFLDa、pFLDα
质粒类型:	酵母表达载体
高拷贝/低拷贝:	高拷贝
启动子:	FLD1
克隆方法:	多克隆位点，限制性内切酶
载体大小:	4694bp
5' 测序引物及序列:	--
3' 测序引物及序列:	--
载体标签:	His Tag (6x) ; V5 Epitope Tag
载体抗性:	Ampicillin
筛选标记:	Zeocin
备注:	用于巴斯德毕赤酵母蛋白表达。含有α因子，是分泌表达载体。诱导剂：甲胺。
产品目录号:	V942-20
稳定性:	--
组成型/诱导型:	诱导型
病毒/非病毒:	非病毒

pFLDalpha质粒图谱 pFLD α载体图谱和pFLDalpha载体序列 pFLD α质粒序列多克隆位点信息

pFLDalpha载体图谱

pFLDalpha质粒 pFLD α载体简介

pFLD and pFLDα are Pichia pastoris expression vectors designed for methylamine-induced expression and rapid protein purification and detection. The vectors feature:

 FLD promoter for inducible expression with methylamine (1)
 C-terminal V5 epitope and polyhistidine (6xHis) region for convenient detection with an Anti-V5 antibody and purification using nickel-chelating resin
 α-factor secretion signal for efficient transport of proteins to the medium (pFLDα only)

pFLDalpha质粒序列 pFLD α载体序列

GCATGCAGGAATCTCTGGCACGGTGCTAATGGTAGTTATCCAACGGAGCTGAGGTAGTCGATATATCTGG
ATATGCCGCCTATAGGATAAAAACAGGAGAGGGTGAACCTTGCTTATGGCTACTAGATTGTTCTTGTACT
CTGAATTCTCATTATGGGAAACTAAACTAATCTCATCTGTGTGTTGCAGTACTATTGAATCGTTGTAGTA
TCTACCTGGAGGGCATTCCATGAATTAGTGAGATAACAGAGTTGGGTAACTAGAGAGAATAATAGACGTA
TGCATGATTACTACACAACGGATGTCGCACTCTTTCCTTAGTTAAAACTATCATCCAATCACAAGATGCG
GGCTGGAAAGACTTGCTCCCGAAGGATAATCTTCTGCTTCTATCTCCCTTCCTCATATGGTTTCGCAGGG
CTCATGCCCCTTCTTCCTTCGAACTGCCCGATGAGGAAGTCCTTAGCCTATCAAAGAATTCGGGACCATC
ATCGATTTTTAGAGCCTTACCTGATCGCAATCAGGATTTCACTACTCATATAAATACATCGCTCAAAGCT
CCAACTTTGCTTGTTCATACAATTCTTGATATTCACACAATTCAACAACTATTTCGAAACGATGAGATTT
CCTTCAATTTTTACTGCTGTTTTATTCGCAGCATCCTCCGCATTAGCTGCTCCAGTCAACACTACAACAG
AAGATGAAACGGCACAAATTCCGGCTGAAGCTGTCATCGGTTACTCAGATTTAGAAGGGGATTTCGATGT
TGCTGTTTTGCCATTTTCCAACAGCACAAATAACGGGTTATTGTTTATAAATACTACTATTGCCAGCATT
GCTGCTAAAGAAGAAGGGGTATCTCTCGAGAAAAGAGAAGCTGAAGCCCAATTGTTCACGTGGCCCAGCC
GGCCGTCTCGGATCGGTACCCGCGGCGGCCGCCAGCTTGGGCCCAATTGGGGTGGTAAGCCTATCCCTAA
CCCTCTCCTCGGTCTCGATTCTACGGGTGTCGACCATCATCATCATCATCATTGAGTTTGTAGCCTTAGA
CATGACTGTTCCTCAGTTCAAGTTGGGCACTTACGAGAAGACCGGTCTTGCTAGATTCTAATCAAGAGGA
TGTCAGAATGCCATTTGCCTGAGAGATGCAGGCTTCATTTTTGATACTTTTTTATTTGTAACCTATATAG
TATAGGATTTTTTTTGTCATTTTGTTTCTTCTCGTACGAGCTTGCTCCTGATCAGCCTATCTCGCAGCTG
ATGAATATCTTGTGGTAGGGGTTTGGGAAAATCATTCGAGTTTGATGTTTTTCTTGGTATTTCCCACTCC
TCTTCAGAGTACAGAAGATTAAGTGAGACCTTCGTTTGTGCGGATCCGGCGCGCCGATCCGCCTGATGCG
GTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATATGGTGCACTCTCAGTACAATCTGCT
CTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCT
GCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCG
TCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAA
TAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTT
CTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAA
AGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTG
TTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTA
CATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATG
AGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTC
GCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGG
CATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTG
ACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTG
ATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAAT
GGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGAC
TGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTG
ATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTC
CCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAG
ATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATT
TAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCC
TTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCT
TTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGG
ATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCT
TCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTA
ATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGT
TACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGAC
CTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCG
GACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCT
GGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGG
GGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTT
GCTCACACACCATAGCTTCAAAATGTTTCTACTCCTTTTTTACTCTTCCAGATTTTCTCGGACTCCGCGC
ATCGCCGTACCACTTCAAAACACCCAAGCACAGCATACTAAATTTCCCCTCTTTCTTCCTCTAGGGTGTC
GTTAATTACCCGTACTAAAGGTTTGGAAAAGAAAAAAGAGACCGCCTCGTTTCTTTTTCTTCGTCGAAAA
AGGCAATAAAAATTTTTATCACGTTTCTTTTTCTTGAAAATTTTTTTTTTTGATTTTTTTCTCTTTCGAT
GACCTCCCATTGATATTTAAGTTAATAAACGGTCTTCAATTTCTCAAGTTTCAGTTTCATTTTTCTTGTT
CTATTACAACTTTTTTTACTTCTTGCTCATTAGAAAGAAAGCATAGCAATCTAATCTAAGGGCGGCGTTG
ACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCATGGCCAA
GTTGACCAGTGCCGTTCCGGTGCTCACCGCGCGCGACGTCGCCGGAGCGGTCGAGTTCTGGACCGACCGG
CTCGGGTTCTCCCGGGACTTCGTGGAGGACGACTTCGCCGGTGTGGTCCGGGACGACGTGACCCTGTTCA
TCAGCGCGGTCCAGGACCAGGTGGTGCCGGACAACACCCTGGCCTGGGTGTGGGTGCGCGGCCTGGACGA
GCTGTACGCCGAGTGGTCGGAGGTCGTGTCCACGAACTTCCGGGACGCCTCCGGGCCGGCCATGACCGAG
ATCGGCGAGCAGCCGTGGGGGCAGGAGTTCGCCCTGCGCGACCCGGCCGGCAACTGCGTGCACTTCGTGG
CCGAGGAGCAGGACTGACACGTCCGACGCGGCCCGACGGGTCCGAGGCCTCGGAGATCCGTCCCCCTTTT
CCTTTGTCGATATCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCCCCCACATCCGCTCTAAC
CGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTA
AGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATAC
TGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGCAAGCTGAATTGCGGCCGCAG
ATCT

pGEX-6p-1-AT5G48650	pGL4.21[luc2P/Puro]
pUTK21	pLVX-AmCyan1-N1
pAAVLINK-EF1a-HA-5'-DIP2C	pCDEF-HA-Ub-K63R
pL-brevisGH1-TA	pcDNA3.1+N-DYK-P2A
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-POGZ-FLAG	pUC19
YEp24	pGEX-6p-1-hsG3BP2b
pSW510	pBluescript II KS(-)
pFR-Luc	pSuper.Retro.Neo
pS-hydro_TA	pMMB66EH
pIEx/Bac-3	p1JI008
p19D+	pHY300PLK
pPM408	pYES2-EGFP
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PHIP-FLAG	pWY113 pET28a_AtmtE2
pAAVLINK-EF1a-5'-PXDN	pcDNA4/TO/Myc-His/LacZ
pAX01-ComK	pBBR1MCS2-Tac-mCherry
pET-53-DEST	pFastBac1-His-C
pGL4.18[luc2P/Neo]	pLC4-14
pAAVLINKv1-pCALM1-Cre-3'-ITSN1-FLAG	pFN10A (ACT)
pBARMTE1	pAAV-CAG-NES-YTnC
pShuttle-CMV	pAAVLINKv1-pCALM1-Cre-3'-RAI1-FLAG
pIZT/V5-His	pBTB-5
pIRESpuro3	pDEST8
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KDM5A-FLAG	pCEP4-Flag-N
FUW-tetO-hOCT4	ppyrG
Pgex-HRV3C	pVK101
pcDNA3.1-CNTRFv1-HA-His	pCRE-DD-AmCyan1
pMAL-p5x	pGFP22
pSP65-F1+	pCEP4-DsRed2
pBABE-HAII	pSwitch
pYC25	pIEX-3
YEp620	pENTR5'/CMVp
pEYFP-Mem	pAAV-EFS-DIO-GRAB_OT1.0
pENTR-L1pLaclacZaL4	pDB262
pRI101-AN	pSEAP2-Basic
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CADPS-FLAG	pDM4
pIEX-1	pQLinkN
pLenti6/V5-GW/lacZ	pBR327
pHisB-Sumo-NeMeHg	pHIL-D2
pDNAPolbeta_TA	pAAV-hSyn-Cepheid1s-WPRE
pcDNA3.1-mRFP	pHBT-GFP-NOS
pRetroX-Tight-Hyg	pHOOK1
pNov.aro-bgl1-640	pHR31
pET28a-mOrange(M163A)	pcDNA6.2/nTC-Tag-DEST
pAMβ1	pCRII-TOPO/22-1
pHT01	pAAVLINK-EF1a-HA-5'-ITSN1
pBad33	pAAVLINK-EF1a-HA-5-RALGAPB
pENTR U6	pAAV-TRE-CAG-Fluc
pMCS-Green-Renilla-Luc	pQE-16
pAP1(PMA)-TA-Luc	YEp16
pcDNA3.1/Zeo(-)	pCold-SUMO

pGEX6p-1-BCCP-PupE	pBiFC-VN155(I152L)
pPB-AMBRA1-3xFLAG	LentiGuide_DKO
pMIH-TOMM20-Halo	pDawn-sfGFP
pX330_SpCas9_USP7 Exon 3 gRNA	pGL3 3'UTR reporter WT 1.3 kb CD274 Hs 3
mAzurite-8xbiRhoBAST-24xMS2v6	pMR-A-3
pTA-HUH	pRK5-HA-Ubiquitin-K29R
AdEasier-1 cells (strain)	pBLO 64.1
pcDNA3 Connexin43-GFP-APEX2	pQCXIP-GFP-LacR
pSI-Target-AID-NG	pLIC-SM-HA
BB3cK_pGAP_23*_pTEF_Cas9	pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA
pAAV2/9n	pQUAST>stop>mCD8-GFP
pcDNA3.1 SIRT6_H133Y	pEGFP-C1 F-tractin-EGFP
pAKTaq	AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-
pJRH-1179 U6-reci Gag-Cas9 v2	pscAAV-CAG-GFP
pCMV-HA-JMJD3	MG1655 ΔendA ΔrecA (DE3)
pMXs-hSOX2	pDriver
pEG302 22aa SunTag NtDRMcd (no NLS) g4+g	pINDUCER21-puro_Gateway-3xFLAG
BCR/ABL P190 transgenic construct	MP6
pBSK	phage UbiC G3BP1-SNAP
pAc-sgRNA-Cas9	pCDH-DCX-TurboID-V5-puro
Fuw-dCas9-Dnmt3a_IM	pT7-hMLH1dn for IVT
pLTR-RD114A	pTM1_VP30_ZEBOV
murine TCR OTI-2A.pMIG II	pKLV2-U6gRNA5(Empty)-PGKBFP2AGFP-W
pKAR2-Br512	CAPTURE2_pLVX-EF1a-dCas9-CBio-IRES-zsGre
pCDNA3 Flag p38 alpha	LeGO-iG
PA-GFPmt	pAAVS1-PC-GCaMP6f
CAMK2D gRNA (BRDN0001146112)	pET16B.Pfu
pCITEp65	pKO (Hyg)
shYAP1	pBIG1a
pCMT-flp	pHCMV_DUGV_GP
pBK092	pET28GST-LIC
pRK5-HA-SH3BP4 W92A	pXR002:EF1a-dCasRx-2A-EGFP
pGP-CMV-Gcamp6m	pQCasTns(Vch)-entry(BsaI)
pcDNA3.1(+) CircRNA Mini Vector	pCS2+HyPer7-NLS
Double UP mNeon to mScarlet	pcDNA3.1_AktPH-mCherry
pcDNA5-T/O-Ds1-mCherry	GW1-Peredox-mCherry-NLS
pEBTet_NAD-Snifit-mito	pT7-SpCas9_sgRNA-site1 (RTW443)
GFP-MAP1B	pLenti-MP2
Myc tag WT PTB	p6XHis-T7(P266L)
CD109-bio-His	EGFP-LC3
pShuttle-CMV-GFP U6promoter	pHIV-Luciferase
pCutamp	pCAG-FAST-p10
SIRT6 Flag	pRH2502
pcDNA3.1-ERBB4	pNG9
PX551	mARG1 cassette 3
pCRISPR-cBEST	pcDNA3.1-TYMS
pCI-6.20	pCMV-mA1-BE3
pEF1a-Usp9x_CD-mCherry	PB533_15F11-sfGFP
pLminP_Luc2P_RE4	GAG-CRErec
pCI-neo.mCherry-NEDD4	pGHYB

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pFLDalpha

买家导航

pFLDalpha载体 pFLD α质粒基本信息

pFLDalpha质粒图谱 pFLD α载体图谱和pFLDalpha载体序列 pFLD α质粒序列多克隆位点信息

pFLDalpha质粒 pFLD α载体简介

pFLDalpha质粒序列 pFLD α载体序列

pFLDalpha载体图谱 pFLD α质粒图谱pdf版和pFLDalpha载体序列 pFLD α质粒序列等相关资料下载

pFLDalpha质粒 pFLD α载体应用举例

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