| 质粒名称: | pN094 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 167155 |
| 存储实验室: | Karen Sarkisyan |
|
载体骨架基本信息 |
|
| 载体名称: | Level1-like (position 6) |
| 空载体大小: | 4456 bp |
| 载体类型: | 植物表达 |
| 载体抗性: | 氨苄青霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | p35s-npgA-ocsT |
| 物种来源: | Aspergillus nidulans |
| 插入大小: | 2198 bp |
| 启动子: | p35s |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | BsaI（在克隆过程中被破坏） |
| 3’克隆位点: | BsaI（在克隆过程中被破坏） |
| 5’测序引物 | ggtggcaggatatattgtggtgtaaac |
| 3’测序引物 | ttcacgcccttttaaatatcc |
|
文献引用方法 |
|
| 材料和方法部分: | pN094 was a gift from Karen Sarkisyan (Addgene plasmid # 167155 ; http://n2t.net/addgene:167155 ; RRID:Addgene_167155) |
| 参考文献部分: | Plants with genetically encoded autoluminescence. Mitiouchkina T, Mishin AS, Somermeyer LG, Markina NM, Chepurnyh TV, Guglya EB, Karataeva TA, Palkina KA, Shakhova ES, Fakhranurova LI, Chekova SV, Tsarkova AS, Golubev YV, Negrebetsky VV, Dolgushin SA, Shalaev PV, Shlykov D, Melnik OA, Shipunova VO, Deyev SM, Bubyrev AI, Pushin AS, Choob VV, Dolgov SV, Kondrashov FA, Yampolsky IV, Sarkisyan KS. Nat Biotechnol. 2020 Apr 27. pii: 10.1038/s41587-020-0500-9. doi: 10.1038/s41587-020-0500-9. 10.1038/s41587-020-0500-9 PubMed 32341562 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pN094 6659 bp ds-DNA circular SYN 17-JUN-2021
DEFINITION MoClo-compatible Level1 (position 6) vector encoding Aspergillus
nidulans 4'-phosphopantetheinyl transferase NpgA under control of
0.4 kb 35S promoter, for expression in plants.
ACCESSION .
VERSION .
KEYWORDS pN094
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6659)
AUTHORS Mitiouchkina T, Mishin AS, Somermeyer LG, Markina NM, Chepurnyh TV,
Guglya EB, Karataeva TA, Palkina KA, Shakhova ES, Fakhranurova LI,
Chekova SV, Tsarkova AS, Golubev YV, Negrebetsky VV, Dolgushin SA,
Shalaev PV, Shlykov D, Melnik OA, Shipunova VO, Deyev SM, Bubyrev
AI, Pushin AS, Choob VV, Dolgov SV, Kondrashov FA, Yampolsky IV,
Sarkisyan KS
TITLE Plants with genetically encoded autoluminescence.
JOURNAL Nat Biotechnol. 2020 Apr 27. pii: 10.1038/s41587-020-0500-9. doi:
10.1038/s41587-020-0500-9.
PUBMED 32341562
REFERENCE 2 (bases 1 to 6659)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jun 17, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6659
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 76..100
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
rep_origin 192..823
/label=oriV
/note="incP origin of replication"
promoter 910..1014
/gene="bla"
/label=AmpR promoter
CDS 1015..1875
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(1233..1252)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 2046..2634
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 2535..2554
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS 2970..4118
/codon_start=1
/product="trans-acting replication protein that binds to
and activates oriV"
/label=trfA
/translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
misc_feature 4274..4298
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
terminator 4347..5054
/label=OCS terminator
/note="octopine synthase terminator"
promoter complement(6204..6549)
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
primer_bind complement(6241..6261)
/label=35S promoter
/note="CaMV 35S promoter, forward primer"
ORIGIN
1 gtggggccca ctgcatccac cccagtacat taaaaacgtc cgcaatgtgt tattaagttg
61 tctaagcgtc aatttgttta caccacaata tatcctgcca ccagccagcc aacagctccc
121 cgaccggcag ctcggcacaa aatcaccact cgatacaggc agcccatcag tcagatcagg
181 atctcctttg cgacgctcac cgggctggtt gccctcgccg ctgggctggc ggccgtctat
241 ggccctgcaa acgcgccaga aacgccgtcg aagccgtgtg cgagacaccg cggccgccgg
301 cgttgtggat acctcgcgga aaacttggcc ctcactgaca gatgaggggc ggacgttgac
361 acttgagggg ccgactcacc cggcgcggcg ttgacagatg aggggcaggc tcgatttcgg
421 ccggcgacgt ggagctggcc agcctcgcaa atcggcgaaa acgcctgatt ttacgcgagt
481 ttcccacaga tgatgtggac aagcctgggg ataagtgccc tgcggtattg acacttgagg
541 ggcgcgacta ctgacagatg aggggcgcga tccttgacac ttgaggggca gagtgctgac
601 agatgagggg cgcacctatt gacatttgag gggctgtcca caggcagaaa atccagcatt
661 tgcaagggtt tccgcccgtt tttcggccac cgctaacctg tcttttaacc tgcttttaaa
721 ccaatattta taaaccttgt ttttaaccag ggctgcgccc tgtgcgcgtg accgcgcacg
781 ccgaaggggg gtgccccccc ttctcgaacc ctcccggccc gctaacgcgg gcctcccatc
841 cccccagggg ctgcgcccct cggccgcgaa cggcctcacc ccaaaaatgg cagcgctggc
901 caattcgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
961 ctcatgagac aataaccttg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
1021 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
1081 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
1141 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
1201 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
1261 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
1321 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
1381 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
1441 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
1501 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
1561 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
1621 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
1681 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg ttctcgcggt
1741 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
1801 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
1861 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
1921 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
1981 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
2041 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
2101 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
2161 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
2221 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
2281 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
2341 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
2401 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
2461 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
2521 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
2581 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
2641 agcaacgcgg cctttttacg gttcctggca gatcctagat gtggcgcaac gatgccggcg
2701 acaagcagga gcgcaccgac ttcttccgca tcaagtgttt tggctctcag gccgaggccc
2761 acggcaagta tttgggcaag gggtcgctgg tattcgtgca gggcaagatt cggaatacca
2821 agtacgagaa ggacggccag acggtctacg ggaccgactt cattgccgat aaggtggatt
2881 atctggacac caaggcacca ggcgggtcaa atcaggaata agggcacatt gccccggcgt
2941 gagtcggggc aatcccgcaa ggagggtgaa tgaatcggac gtttgaccgg aaggcataca
3001 ggcaagaact gatcgacgcg gggttttccg ccgaggatgc cgaaaccatc gcaagccgca
3061 ccgtcatgcg tgcgccccgc gaaaccttcc agtccgtcgg ctcgatggtc cagcaagcta
3121 cggccaagat cgagcgcgac agcgtgcaac tggctccccc tgccctgccc gcgccatcgg
3181 ccgccgtgga gcgttcgcgt cgtcttgaac aggaggcggc aggtttggcg aagtcgatga
3241 ccatcgacac gcgaggaact atgacgacca agaagcgaaa aaccgccggc gaggacctgg
3301 caaaacaggt cagcgaggcc aagcaggccg cgttgctgaa acacacgaag cagcagatca
3361 aggaaatgca gctttccttg ttcgatattg cgccgtggcc ggacacgatg cgagcgatgc
3421 caaacgacac ggcccgctct gccctgttca ccacgcgcaa caagaaaatc ccgcgcgagg
3481 cgctgcaaaa caaggtcatt ttccacgtca acaaggacgt gaagatcacc tacaccggcg
3541 tcgagctgcg ggccgacgat gacgaactgg tgtggcagca ggtgttggag tacgcgaagc
3601 gcacccctat cggcgagccg atcaccttca cgttctacga gctttgccag gacctgggct
3661 ggtcgatcaa tggccggtat tacacgaagg ccgaggaatg cctgtcgcgc ctacaggcga
3721 cggcgatggg cttcacgtcc gaccgcgttg ggcacctgga atcggtgtcg ctgctgcacc
3781 gcttccgcgt cctggaccgt ggcaagaaaa cgtcccgttg ccaggtcctg atcgacgagg
3841 aaatcgtcgt gctgtttgct ggcgaccact acacgaaatt catatgggag aagtaccgca
3901 agctgtcgcc gacggcccga cggatgttcg actatttcag ctcgcaccgg gagccgtacc
3961 cgctcaagct ggaaaccttc cgcctcatgt gcggatcgga ttccacccgc gtgaagaagt
4021 ggcgcgagca ggtcggcgaa gcctgcgaag agttgcgagg cagcggcctg gtggaacacg
4081 cctgggtcaa tgatgacctg gtgcattgca aacgctaggg ccttgtgggg tcagttccgg
4141 ctgggggttc agcagccagc gcctgatctg gggaaccctg tggttggcat gcacatacaa
4201 atggacgaac ggataaacct tttcacgccc ttttaaatat ccgattattc taataaacgc
4261 tcttttctct taggtttacc cgccaatata tcctgtcaaa cactgatagt ttaaaccact
4321 tcgtgcagaa gacaagctca gcggtcctgc tgagcctcga catgttgtcg caaaattcgc
4381 cctggacccg cccaacgatt tgtcgtcact gtcaaggttt gacctgcact tcatttgggg
4441 cccacataca ccaaaaaaat gctgcataat tctcggggca gcaagtcggt tacccggccg
4501 ccgtgctgga ccgggttgaa tggtgcccgt aactttcggt agagcggacg gccaatactc
4561 aacttcaagg aatctcaccc atgcgcgccg gcggggaacc ggagttccct tcagtgagcg
4621 ttattagttc gccgctcggt gtgtcgtaga tactagcccc tggggcactt ttgaaatttg
4681 aataagattt atgtaatcag tcttttaggt ttgaccggtt ctgccgcttt ttttaaaatt
4741 ggatttgtaa taataaaacg caattgtttg ttattgtggc gctctatcat agatgtcgct
4801 ataaacctat tcagcacaat atattgtttt cattttaata ttgtacatat aagtagtagg
4861 gtacaatcag taaattgaac ggagaatatt attcataaaa atacgatagt aacgggtgat
4921 atattcatta gaatgaaccg aaaccggcgg taaggatctg agctacacat gctcaggttt
4981 tttacaacgt gcacaacaga attgaaagca aatatcatgc gatcataggc ttctcgcata
5041 tctcattaaa gcaggaccta ttaagctagt taactacctc gagacagaca attacagact
5101 ccagtggcgc aaggctgtat gtctctctct atgtcgagct ttttaaatgg tcgccaggga
5161 tcttgactcc taattcctga gccatcaggg cctccccctg gtcggctgga ggcacctata
5221 ccgccccctc gtgcagcagt ggcgaacaag tagtcacctc ccagagcagc cacctctatc
5281 ctaacatcct cgaccaagtt tttgtaaagg gttgttcgta ccccggtata aggctctcca
5341 aagtctccgg cggaatctcc ggattcggcc actgctgctg gggcaacgac attactaaat
5401 tccaactccc tgagccatgg tgccagcagg gcttcccctg tcatcttgat gtatgcttcc
5461 ttaagagccc aataagtgta aaacaatcgg taaccatagt ccaccaacct atcggcgctc
5521 aaggagcttg atgacacccc atccaatctt ctgatgtttg ccatctctgc ggtggaaaaa
5581 acctctgaga aaatatctat gtactgcctc aaactttcaa gggatcgctc ctcaccgttt
5641 cgcccctgtc gctcattcac gcaagtaatg tctatgccaa cctctggctt aagtttggaa
5701 tcacctccag agttgggtgt aaatgcagtg cctgctattg ccaccatgct tgcttgatgg
5761 gatacgttga actcgacatt aattccagtg tagccatcct taaagctgtc ctcttgagaa
5821 ccagagggtg gtatataaca gggccttcta tggggatctg gagttcttga tattacaatg
5881 ctagaccatg ggattcggca attcctgtga acaaacaagt acttgagaag attggatgcg
5941 agagacatat gcttatcctt cagatggtag tacttttgga cacttatctg gtctgcaggc
6001 tgcagagttt ccaacagtgg caaggctgcg gtagaagcag tcaaaggtct agtatctata
6061 taccatcttg tcaaaattgg actggtacta gcgcttgagg tgtcttggac cattgtatcg
6121 ataattgtaa atgtaattgt aatgttgttt gttgtttgtt gttgttggta attgttgtaa
6181 aaatgagctc ttatactcga gcgtgtcctc tccaaatgaa atgaacttcc ttatatagag
6241 gaagggtctt gcgaaggata gtgggattgt gcgtcatccc ttacgtcagt ggagatgtca
6301 catcaatcca cttgctttgt agacgtggtt ggaacctctt ctttttccac gatgctcctc
6361 gtgggtgggg gtccatcttt gggaccactg tcggcagaga gatcttgaat gatagccttt
6421 cctttatcgc aatgatggca tttgtaggag ccaccttcct tttctactgt cctttcgatg
6481 aagtgacaga tagctgggca atggaatccg aggaggtttc ccgaaattat cctttgttga
6541 aaagtctcaa tagccctttg atcttctgag actgtatctt tgacattttt ggagtagacc
6601 agagtgtcgt gctccaccat gttgacctcc ctcgaggaat tccacattgt cttcacaga
//
