pSUPERIOR puro

价格:1500元

联系方式:I47-825O-882O

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pSUPERIORpuro载体基本信息

出品公司: OligoEngine
载体名称: pSUPERIOR puro
质粒类型: 哺乳动物细胞载体;RNAi
高拷贝/低拷贝: --
克隆方法: 限制性内切酶,多克隆位点
启动子: H1
载体大小: 4354kb
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄青霉素
筛选标记: 嘌呤霉素(Puromycin)
诱导物:  
病毒/非病毒: --

pSUPERIORpuro载体质粒图谱和多克隆位点信息

pSUPERIOR puro载体图谱

pSUPERIORpuro载体简介

pSUPERIORpuro载体序列

LOCUS       pSUPERIOR.puro	4354 bp 	DNA	SYN
DEFINITION  pSUPERIOR.puro
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="pSUPERIOR.puro"
                     /mol_type="other DNA"
     rep_origin      complement(135..441)
                     /label="f1_origin"
     CDS             complement(241..1599)
                     /label="ORF frame 2"
     misc_feature    complement(459..614)
                     /label="lacZ_a"
     misc_feature    585..607
                     /label="M13_pUC_fwd_primer"
     promoter        600..616
                     /label="M13_forward20_primer"
     promoter        626..644
                     /label="T7_promoter"
     misc_feature    677..693
                     /label="pBluescriptSK_primer"
     CDS             705..1397
                     /label="ORF frame 3"
     gene            complement(756..1355)
                     /label="puro (variant)"
                     /gene="puro (variant)"
     CDS             complement(756..1355)
                     /label="ORF frame 3"
     misc_feature    complement(1428..1447)
                     /label="mPGK_F_primer"
     misc_feature    1821..1844
                     /label="MSCV_rev_primer"
     misc_feature    2025..2044
                     /label="H1_primer"
     misc_feature    complement(2121..2137)
                     /label="pBluescriptKS_primer"
     promoter        complement(2165..2184)
                     /label="T3_promoter"
     promoter        complement(2201..2219)
                     /label="M13_reverse_primer"
     misc_feature    complement(2218..2240)
                     /label="M13_pUC_rev_primer"
     promoter        complement(2254..2283)
                     /label="lac_promoter"
     gene            complement(3366..4226)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(3366..4226)
                     /label="ORF frame 3"
     promoter        complement(4268..4296)
                     /label="AmpR_promoter"
ORIGIN
    1 CTAAATTGTA AGCGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC
   61 ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA
  121 GATAGGGTTG AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC
  181 CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC
  241 CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG
  301 CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AAGGGAAGAA
  361 AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC
  421 CACACCCGCC GCGCTTAATG CGCCGCTACA GGGCGCGTCC CATTCGCCAT TCAGGCTGCG
  481 CAACTGTTGG GAAGGGCGAT CGGTGCGGGC CTCTTCGCTA TTACGCCAGC TGGCGAAAGG
  541 GGGATGTGCT GCAAGGCGAT TAAGTTGGGT AACGCCAGGG TTTTCCCAGT CACGACGTTG
  601 TAAAACGACG GCCAGTGAGC GCGCGTAATA CGACTCACTA TAGGGCGAAT TGGAGCTCCA
  661 CCGCGGTGGC GGCCGCTCTA GAACTAGTGG ATCCCCCGGG CTGCATGGGG TCGTGCGCTC
  721 CTTTCGGTCG GGCGCTGCGG GTCGTGGGGC GGGCGTCAGG CACCGGGCTT GCGGGTCATG
  781 CACCAGGTGC GCGGTCCTTC GGGCACCTCG ACGTCGGCGG TGACGGTGAA GCCGAGCCGC
  841 TCGTAGAAGG GGAGGTTGCG GGGCGCGGAG GTCTCCAGGA AGGCGGGCAC CCCGGCGCGC
  901 TCGGCCGCCT CCACTCCGGG GAGCACGACG GCGCTGCCCA GACCCTTGCC CTGGTGGTCG
  961 GGCGAGACGC CGACGGTGGC CAGGAACCAC GCGGGCTCCT TGGGCCGGTG CGGCGCCAGG
 1021 AGGCCTTCCA TCTGTTGCTG CGCGGCCAGC CGGGAACCGC TCAACTCGGC CATGCGCGGG
 1081 CCGATCTCGG CGAACACCGC CCCCGCTTCG ACGCTCTCCG GCGTGGTCCA GACCGCCACC
 1141 GCGGCGCCGT CGTCCGCGAC CCACACCTTG CCGATGTCGA GCCCGACGCG CGTGAGGAAG
 1201 AGTTCTTGCA GCTCGGTGAC CCGCTCGATG TGGCGGTCCG GATCGACGGT GTGGCGCGTG
 1261 GCGGGGTAGT CGGCGAACGC GGCGGCGAGG GTGCGTACGG CCCTGGGGAC GTCGTCGCGG
 1321 GTGGCGAGGC GCACCGTGGG CTTGTACTCG GTCATGGTAA GCTAGCTTGG GCTGCAGGTC
 1381 GAAAGGCCCG GAGATGAGGA AGAGGAGAAC AGCGCGGCAG ACGTGCGCTT TTGAAGCGTG
 1441 CAGAATGCCG GGCCTCCGGA GGACCTTCGG GCGCCCGCCC CGCCCCTGAG CCCGCCCCTG
 1501 AGCCCGCCCC CGGACCCACC CCTTCCCAGC CTCTGAGCCC AGAAAGCGAA GGAGCAAAGC
 1561 TGCTATTGGC CGCTGCCCCA AAGGCCTACC CGCTTCCATT GCTCAGCGGT GCTGTCCATC
 1621 TGCACGAGAC TAGTGAGACG TGCTACTTCC ATTTGTCACG TCCTGCACGA CGCGAGCTGC
 1681 GGGGCGGGGG GGAACTTCCT GACTAGGGGA GGAGTAGAAG GTGGCGCGAA GGGGCCACCA
 1741 AAGAACGGAG CCGGTTGGCG CCTACCGGTG GATGTGGAAT GTGTGCGAGG CCAGAGGCCA
 1801 CTTGTGTAGC GCCAAGTGCC CAGCGGGGCT GCTAAAGCGC ATGCTCCAGA CTGCCTTGGG
 1861 AAAAGCGCCT CCCCTACCCG GTAGAATTCG AACGCTGACG TCATCAACCC GCTCCAAGGA
 1921 ATCGCGGGCC CAGTGTCACT AGGCGGGAAC ACCCAGCGCG CGTGCGCCCT GGCAGGAAGA
 1981 TGGCTGTGAG GGACAGGGGA GTGGCGCCCT GCAATATTTG CATGTCGCTA TGTGTTCTGG
 2041 GAAATCACCA TAAACGTGAA ATGTCTTTGG ATTTGGGAAT CTTATAAGTT CCCTATCAGT
 2101 GATAGAGATC TAAGCTTATC GATACCGTCG ACCTCGAGGG GGGGCCCGGT ACCCAGCTTT
 2161 TGTTCCCTTT AGTGAGGGTT AATTGCGCGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT
 2221 GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT
 2281 AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC
 2341 GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG
 2401 AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG
 2461 GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA
 2521 GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC
 2581 CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC
 2641 AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG
 2701 TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC
 2761 CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT
 2821 CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG
 2881 CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC
 2941 TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT
 3001 GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT
 3061 ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC
 3121 AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA
 3181 AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC
 3241 GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC
 3301 CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT
 3361 GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA
 3421 TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT
 3481 GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA
 3541 ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC
 3601 ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG
 3661 CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT
 3721 TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA
 3781 AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA
 3841 TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC
 3901 TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG
 3961 AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA
 4021 GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG
 4081 AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC
 4141 ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG
 4201 GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT
 4261 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA
 4321 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCAC
//

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