| 质粒名称: | pCCLc-MND-B2705-SABR-Backbone |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 119051 |
| 存储实验室: | David Baltimore |
|
载体骨架基本信息 |
|
| 载体名称: | pCCLc-MND |
| 载体出品公司: | Donald B. Kohn's laboratory at UCLA |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 6600 bp |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 筛选标记: | 表面myc标签 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 5' 测序引物 | GCTCCCCGAGCTCAATAAAAG |
| 3' 测序引物 | TGGCTAAGATCTACAGCTGCCTT |
|
文献引用方法 |
|
| 材料和方法部分: | pCCLc-MND-B2705-SABR-Backbone was a gift from David Baltimore (Addgene plasmid # 119051 ; http://n2t.net/addgene:119051 ; RRID:Addgene_119051) |
| 参考文献部分: | T cell antigen discovery via signaling and antigen-presenting bifunctional receptors. Joglekar AV, Leonard MT, Jeppson JD, Swift M, Li G, Wong S, Peng S, Zaretsky JM, Heath JR, Ribas A, Bethune MT & Baltimore D.. Nature Methods (2019) volume 16, pages 191–198. 10.1038/s41592-018-0304-8 |
买家导航
pCCLc-MND-B2705-SABR-Backbone载体质粒基本信息
pCCLc-MND-B2705-SABR-Backbone质粒图谱载体图谱和pCCLc-MND-B2705-SABR-Backbone载体序列质粒序列多克隆位点信息
pCCLc-MND-B2705-SABR-Backbone质粒载体简介
pCCLc-MND-B2705-SABR-Backbone质粒序列载体序列
LOCUS pCCLc-MND-B2705-SABR-Backbone 10900 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION 3rd gen transfer vector. HLA-B2705 linked to b2-microglobulin and
CD28-CD3z signaling domain. Contains a stuffer fragment flanked by
BsmBI sites at the epitope cloning site..
ACCESSION .
VERSION .
KEYWORDS pCCLc-MND-B2705-SABR-Backbone
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10900)
AUTHORS Joglekar AV, Leonard MT, Jeppson JD, Swift M, Li G, Wong S, Peng S,
TITLE T cell antigen discovery via signaling and antigen-presenting
bifunctional receptors
JOURNAL Nature Methods (2019) volume 16, pages 191–198.
REFERENCE 2 (bases 1 to 10900)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10900
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 1..22
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 37..67
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 75..91
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 80..102
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 99..115
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 99..115
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 134..154
/label=T3
/note="T3 promoter, forward primer"
promoter 136..154
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
enhancer 255..634
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 635..838
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 789..809
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 853..1033
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 1080..1205
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1698..1931
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 2116..2160
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2457..2574
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
CDS 7038..7103
/codon_start=1
/product="2A peptide from foot-and-mouth disease virus
polyprotein"
/label=F2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="VKQTLNFDLLKLAGDVESNPGP"
CDS 7188..7217
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
LTR 7600..7833
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 7905..8026
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7942..7961)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 7996..8015
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 8066..8201
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 8128..8147
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(8221..8240)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(8222..8240)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(8250..8267)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(8250..8266)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8259..8281)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 8408..8863
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(8495..8514)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 8705..8726
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 8889..8993
/gene="bla"
/label=AmpR promoter
CDS 8994..9854
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(9212..9231)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 10025..10613
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 10514..10533
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 10767..10784
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagcttg
181 gccattgcat acgttgtatc catatcataa tatgtacatt tatattggct catgtccaac
241 attaccgcca tgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
301 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
361 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
421 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
481 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
541 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
601 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
661 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
721 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
781 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg
841 tttagtgaac cggggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
901 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
961 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
1021 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
1081 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
1141 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
1201 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
1261 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
1321 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
1381 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
1441 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
1501 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga
1561 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
1621 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
1681 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
1741 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
1801 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
1861 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
1921 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
1981 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
2041 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
2101 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
2161 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
2221 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
2281 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
2341 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
2401 aacggatctc gacggtatcg ataagctaat tcacaaatgg cagtattcat ccacaatttt
2461 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
2521 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
2581 tattacaggg acagcagaga tccagtttgg gaattagctt gatcgattag tccaatttgt
2641 taaagacagg atatcagtgg tccaggctct agttttgact caacaatatc accagctgaa
2701 gcctatagag tacgagccat agatagaata aaagatttta tttagtctcc agaaaaaggg
2761 gggaatgaaa gaccccacct gtaggtttgg caagctagga tcaaggttag gaacagagag
2821 acagcagaat atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg
2881 ccaagaacag ttggaacagc agaatatggg ccaaacagga tatctgtggt aagcagttcc
2941 tgccccggct cagggccaag aacagatggt ccccagatgc ggtcccgccc tcagcagttt
3001 ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta
3061 tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct
3121 caataaaaga gcccacaacc cctcactcgg cgcgatctag atctcgaatc gaattcatgg
3181 cgacgggttc aagaacttcc ctacttcttg catttggcct gctttgtttg ccgtggttac
3241 aggagggctc ggcaggagac ggttgtaaat gagcacacaa aatacacatg ctaaaatatt
3301 atattctatg acctttataa aatcaaccaa aatcttcttt ttaataactt tagtatcaat
3361 aattagaatt tttatgttcc tttttgcaaa cttttaataa aaatgagcaa aataaaaaaa
3421 cgctagtttt agtaactcgc gttgttttct tcacctttaa taatagctac tccaccactt
3481 gttcctaagc ggtcagctcc tgcttcaatc attttttgag catcttcaaa tgttctaact
3541 ccaccagctg ctttaactaa agcattgtct ttaacaactg acttcattag tttaacatct
3601 tcaaatgttg cacctgattt tgaaaatcct gttgatgttt taacaaattc taatccagct
3661 tcaacagcta tttcacaagc tttcatgatt tcttcttttg ttaataaaca attttccata
3721 atacatttaa caacatgtga tccagctgct ttttttacag ctttcatgtc ttctaaaact
3781 aattcataat ttttgtcttt taatgcacca atatttaata ccatatcaat ttctgttgca
3841 ccatctttaa ttgcttcaga aacttcgaat gcttttgtag ctgttgtgca tgcacctaga
3901 ggaaaaccta caacatttgt tattcctaca tttgtgcctt ttaataattc tttacaatag
3961 cttgttcaat atgaattaac acaaactgtt gcaaaatcaa attcaattgc ttcatcacat
4021 aattgtttaa tttcagcttt cgtagcatct tgttttaata atgtgtgatc tatatatttg
4081 tttagtttca ttttttctcc tatatattca tttttaattt taattcttta ataatttcgt
4141 ctactttaac tttagcgttt tgaacagatt caccaacacc tataaaataa atttttagtt
4201 taggttcagt tccacttggg cgaacagcaa atcatgactt atcttctaaa taaaatttta
4261 gtaagtcttg tcctggcata ttatacattc catcgatgta gtcttcaaca ttaacaactt
4321 taagtccagc aatttgagtt aagggtgttg ctctcaatga tttcattaat ggttcaattt
4381 ttaatttctt ttcttctggt ttaaaattca agtttaaagt gaaagtgtaa tatgcaccca
4441 tttctttaaa taaatcttct aaatagtcta ctaatgtttt attttgtttt ttataaaatc
4501 aagcagcctc tgctattaat atagaagctt gtattccatc tttatctcta gctgagtcat
4561 caattacata tccataactt tcttcataag caaaaacaaa atttaatccg ttatcttctt
4621 ctttagcaat ttctctaccc attcatttaa atccagttaa agtttttaca atattaactc
4681 catatttttc atgagcgatt ctatcaccca aatcacttgt tacaaaactt gaatatagag
4741 ccggattttt tggaatgcta tttaagcgtt ttagatttga taattttcaa tcaattaaaa
4801 ttggtcctgt ttgatttcca tctaatctta caaaatgacc atcatgtttt attgccattc
4861 caaatctgtc agcatctggg tcattcataa taataatatc tgcatcatgt ttaataccat
4921 attcaagcgg tatttttcat gcaggatcaa attctggatt tggatttaca acatttttaa
4981 atgtttcatc ttcaaatgca tgctcttcaa cctcaataac gttatatcct gattcacgta
5041 atatttttgg ggtaaattta gttcctgttc cattaactgc gctaaaaata atttttaaat
5101 cttttttagc ttcttgctct tttttgtacg tctctggatg cggagggtcc ggcgggggag
5161 gtagcggtgg tggaggaagc atccagcgta ctccaaagat tcaggtttac tcacgtcatc
5221 cagcagagaa tggaaagtca aatttcctga attgctatgt gtctgggttt catccatccg
5281 acattgaagt tgacttactg aagaatggag agagaattga aaaagtggag cattcagact
5341 tgtctttcag caaggactgg tctttctatc tcttgtacta cactgaattc acccccactg
5401 aaaaagatga gtatgcctgc cgtgtgaacc atgtgacttt gtcacagccc aagatagtta
5461 agtgggatcg agacatggga ggtggtggca gcggtggagg gggctccggc ggtgggggat
5521 ccggcggcgg cggaagcggc tcccactcca tgaggtattt ccacacctcc gtgtcccggc
5581 ccggccgcgg ggagccccgc ttcatcaccg tgggctacgt ggacgacacg ctgttcgtga
5641 ggttcgacag cgacgccgcg agtccgagag aggagccgcg ggcgccgtgg atagagcagg
5701 aggggccgga gtattgggac cgggagacac agatctgcaa ggccaaggca cagactgacc
5761 gagaggacct gcggaccctg ctccgctgct acaaccagag cgaggccggg tctcacaccc
5821 tccagaatat gtatggctgc gacgtggggc cggacgggcg cctcctccgc gggtaccacc
5881 aggacgccta cgacggcaag gattacatcg ccctgaacga ggacctgagc tcctggaccg
5941 ccgcggacac ggcggctcag atcacccagc gcaagtggga ggcggcccgt gtggcggagc
6001 agctgagagc ctacctggag ggcgagtgcg tggagtggct ccgcagatac ctggagaacg
6061 ggaaagaaac actgcagcgc gcggaccccc caaagacaca cgtgacccac caccccatct
6121 ctgaccatga ggccaccctg aggtgctggg ccctgggctt ctaccctgcg gagatcacac
6181 tgacctggca gcgggatggc gaggaccaaa ctcaggacac tgagcttgtg gagaccagac
6241 cagcaggaga tagaaccttc cagaagtggg cagctgtggt ggtgccttct ggagaagagc
6301 agagatacac atgccatgta cagcatgagg ggctgccgaa gcccctcacc ctgagatggg
6361 agccgtcttc ccagtccacc gtccccatcg tgggcattgt tgctggcctg gctgtcctag
6421 cagttgtggt catcggagct gtggtcgctg ctgtgatgtg taggaggaag agctcaggtg
6481 gaaaaggagg gagctactct caggctgcgt gcagcgacag tgcccagggc tctgatgtgt
6541 ctctcacagc tcggagcaag aggtctcgcg gtgggcattc cgactacatg aacatgaccc
6601 ctagaaggcc tggcccaacc agaaagcact accagccata cgcccctccc agagatttcg
6661 ccgcttatcg aagcgtgaag ttctcccgaa gcgcagatgc cccagcctat cagcagggac
6721 agaatcagct gtacaacgag ctgaacctgg gaagacggga ggaatacgat gtgctggaca
6781 aaaggcgggg cagagatcct gagatgggcg gcaaaccaag acggaagaac ccccaggaag
6841 gtctgtataa tgagctgcag aaagacaaga tggctgaggc ctactcagaa atcgggatga
6901 agggcgaaag aaggagagga aaaggccacg acggactgta ccaggggctg agtacagcaa
6961 caaaagacac ctatgacgct ctgcacatgc aggctctgcc accaagaagg gcaaaacgtt
7021 cgggttcggg tgcgccagta aagcagacat taaactttga tttgctgaaa cttgcaggtg
7081 atgtagagtc aaatccaggt ccaggggcag gtgccaccgg ccgcgccatg gacgggccgc
7141 gcctgctgct gttgctgctt ctgggggtgt cccttggagg tgccaaggag cagaagctga
7201 tcagcgagga ggacctggag atccctggcc gttggattac acggtccaca cccccagagg
7261 gctcggacag cacagccccc agcacccagg agcctgaggc acctccagaa caagacctca
7321 tagccagcac ggtggcaggt gtggtgacca cagtgatggg cagctcccag cccgtggtga
7381 cccgaggcac caccgacaac ctcatccctg tctattgctc catcctggct gctgtggttg
7441 tgggtcttgt ggcctacata gccttcaaga ggtgagaatt cgttaacctc gagggatccc
7501 ccggggtcga ctgatcaaat tcgagctcgg tacctttaag accaatgact tacaaggcag
7561 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
7621 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
7681 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
7741 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
7801 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
7861 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
7921 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
7981 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
8041 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
8101 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
8161 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
8221 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
8281 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
8341 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
8401 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
8461 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
8521 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
8581 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
8641 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
8701 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
8761 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
8821 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
8881 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
8941 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
9001 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
9061 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
9121 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
9181 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
9241 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
9301 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
9361 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
9421 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
9481 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
9541 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
9601 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
9661 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
9721 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
9781 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
9841 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
9901 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
9961 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
10021 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
10081 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
10141 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
10201 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
10261 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
10321 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
10381 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
10441 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
10501 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
10561 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
10621 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
10681 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
10741 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
10801 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
10861 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
//
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pCCLc-MND-B2705-SABR-Backbone质粒载体应用举例
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