| 质粒名称: | pLminP_Luc2P_RE23 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 90365 |
| 存储实验室: | Ramnik Xavier |
|
载体骨架基本信息 |
|
| 载体名称: | pLenti7.3 |
| 载体出品公司: | Thermo Fisher |
| 空载体大小: | 7251bp |
| 完整质粒大小: | 9293bp |
| 载体类型: | Lentiviral |
| 筛选标记: | seGFP |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Firefly luciferase、Luc2P |
| 物种来源: | P. pyralis |
| 插入大小: | 1776bp |
| 启动子: | AARE |
| 克隆方法: | Gibson Cloning |
| 5’测序引物 | GGTACAGTGCAGGGGAAAGA |
|
文献引用方法 |
|
| 材料和方法部分: | pLminP_Luc2P_RE23 was a gift from Ramnik Xavier (Addgene plasmid # 90365 ; http://n2t.net/addgene:90365 ; RRID:Addgene_90365) |
| 参考文献部分: | Simultaneous Pathway Activity Inference and Gene Expression Analysis Using RNA Sequencing. O'Connell DJ, Kolde R, Sooknah M, Graham DB, Sundberg TB, Latorre IJ, Mikkelsen TS, Xavier RJ. Cell Syst. 2016 May 25;2(5):323-34. doi: 10.1016/j.cels.2016.04.011. Epub 2016 May 19. 10.1016/j.cels.2016.04.011 PubMed 27211859 |
买家导航
pLminP_Luc2P_RE23载体质粒基本信息
pLminP_Luc2P_RE23质粒图谱载体图谱和pLminP_Luc2P_RE23载体序列质粒序列多克隆位点信息
pLminP_Luc2P_RE23质粒载体简介
pLminP_Luc2P_RE23质粒序列载体序列
LOCUS pLminP_Luc2P_RE23 9213 bp DNA circular SYN 15-DEC-2025
DEFINITION ATF4 - Amino acid deprivation gene reporter.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9213)
AUTHORS O'Connell DJ, Kolde R, Sooknah M, Graham DB, Sundberg TB, Latorre
IJ, Mikkelsen TS, Xavier RJ
TITLE Simultaneous Pathway Activity Inference and Gene Expression Analysis
Using RNA Sequencing.
JOURNAL Cell Syst. 2016 May 25;2(5):323-34. doi: 10.1016/j.cels.2016.04.011.
Epub 2016 May 19.
PUBMED 27211859
REFERENCE 2 (bases 1 to 9213)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Dec 15, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1016/j.cels.2016.04.011"; journalName: "Cell Syst"; date:
"2016-05-25- 25"; volume: "2"; issue: "5"; pages: "323-34"
COMMENT Sequence Label: pLminP_Luc2P_RE23
FEATURES Location/Qualifiers
source 1..9213
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 3..229
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 230..410
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 457..582
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1075..1308
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1493..1537
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 1804..1921
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2045..2076
/label=minP
/note="minimal TATA-box promoter with low basal activity"
regulatory 2103..2112
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 2109..3758
/codon_start=1
/product="firefly luciferase"
/label=luciferase
/note="synthetic luc2 version of the luciferase gene"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
CDS 3762..3881
/codon_start=1
/product="PEST degradation sequence from mouse ornithine
decarboxylase"
/label=hPEST
/note="human codon-optimized"
/translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
misc_feature 3973..4561
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(4026..4046)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(4444..4455)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
promoter 4576..4892
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 4743..4878
/label=SV40 ori
/note="SV40 origin of replication"
CDS join(4943..4945,4946..4948,4949..5662)
/codon_start=1
/product="Emerald GFP"
/label=EmGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(4988..5009)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(5249..5268)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 5596..5617
/label=EGFP-C
/note="EGFP, forward primer"
LTR 5734..5967
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 6039..6160
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(6076..6095)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 6130..6149
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 6200..6335
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind complement(6355..6374)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(6356..6374)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(6384..6401)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(6384..6400)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6393..6415)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 6542..6997
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6629..6648)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6839..6860
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 7023..7127
/gene="bla"
/label=AmpR promoter
CDS join(7128..7196,7197..7988)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(7346..7365)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 8159..8747
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8648..8667
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 8901..8918
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 9035..9056
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter join(9071..9076,9077..9094,9095..9101)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 9109..9125
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 9114..9136
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 9133..9149
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 9133..9149
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 9168..9188
/label=T3
/note="T3 promoter, forward primer"
promoter 9170..9188
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
1921 tatcgatcgc accagcgtgt ggatcaacat tgcatcatcc ccgcaacatt gcatcatccc
1981 cgcaacattg catcatcccc gcgatccgag aacagatctg gcctcggcgg ccaagcttag
2041 acactagagg gtatataatg gaagctcgac ttccagcttg gcaatccggt actgttggta
2101 aagccaccat ggaagatgcc aaaaacatta agaagggccc agcgccattc tacccactcg
2161 aagacgggac cgccggcgag cagctgcaca aagccatgaa gcgctacgcc ctggtgcccg
2221 gcaccatcgc ctttaccgac gcacatatcg aggtggacat tacctacgcc gagtacttcg
2281 agatgagcgt tcggctggca gaagctatga agcgctatgg gctgaataca aaccatcgga
2341 tcgtggtgtg cagcgagaat agcttgcagt tcttcatgcc cgtgttgggt gccctgttca
2401 tcggtgtggc tgtggcccca gctaacgaca tctacaacga gcgcgagctg ctgaacagca
2461 tgggcatcag ccagcccacc gtcgtattcg tgagcaagaa agggctgcaa aagatcctca
2521 acgtgcaaaa gaagctaccg atcatacaaa agatcatcat catggatagc aagaccgact
2581 accagggctt ccaaagcatg tacaccttcg tgacttccca tttgccaccc ggcttcaacg
2641 agtacgactt cgtgcccgag agcttcgacc gggacaaaac catcgccctg atcatgaaca
2701 gtagtggcag taccggattg cccaagggcg tagccctacc gcaccgcacc gcttgtgtcc
2761 gattcagtca tgcccgcgac cccatcttcg gcaaccagat catccccgac accgctatcc
2821 tcagcgtggt gccatttcac cacggcttcg gcatgttcac cacgctgggc tacttgatct
2881 gcggctttcg ggtcgtgctc atgtaccgct tcgaggagga gctattcttg cgcagcttgc
2941 aagactataa gattcaatct gccctgctgg tgcccacact atttagcttc ttcgctaaga
3001 gcactctcat cgacaagtac gacctaagca acttgcacga gatcgccagc ggcggggcgc
3061 cgctcagcaa ggaggtaggt gaggccgtgg ccaaacgctt ccacctacca ggcatccgcc
3121 agggctacgg cctgacagaa acaaccagcg ccattctgat cacccccgaa ggggacgaca
3181 agcctggcgc agtaggcaag gtggtgccct tcttcgaggc taaggtggtg gacttggaca
3241 ccggtaagac actgggtgtg aaccagcgcg gcgagctgtg cgtccgtggc cccatgatca
3301 tgagcggcta cgttaacaac cccgaggcta caaacgctct catcgacaag gacggctggc
3361 tgcacagcgg cgacatcgcc tactgggacg aggacgagca cttcttcatc gtggaccggc
3421 tgaagagcct gatcaaatac aagggctacc aggtagcccc agccgaactg gagagcatcc
3481 tgctgcaaca ccccaacatc ttcgacgccg gggtcgccgg cctgcccgac gacgatgccg
3541 gcgagctgcc cgccgcagtc gtcgtgctgg aacacggtaa aaccatgacc gagaaggaga
3601 tcgtggacta tgtggccagc caggttacaa ccgccaagaa gctgcgcggt ggtgttgtgt
3661 tcgtggacga ggtgcctaaa ggactgaccg gcaagttgga cgcccgcaag atccgcgaga
3721 ttctcattaa ggccaagaag ggcggcaaga tcgccgtgaa ttctcacggc ttccctcccg
3781 aggtggagga gcaggccgcc ggcaccctgc ccatgagctg cgcccaggag agcggcatgg
3841 atagacaccc tgctgcttgc gccagcgcca ggatcaacgt ctaaggccgc gactctagaa
3901 cgcgtcactg cggctgtcga atggcattac gggactacag acatcagcgc gtaccggtta
3961 gtaatgatcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
4021 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
4081 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
4141 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
4201 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
4261 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
4321 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt
4381 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
4441 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
4501 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
4561 cctggcgatg gtaccggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
4621 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
4681 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
4741 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
4801 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
4861 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
4921 tcggatctga tcagcaccta gcatggtgag caagggcgag gagctgttca ccggggtggt
4981 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
5041 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
5101 gctgcccgtg ccctggccca ccctcgtgac caccttcacc tacggcgtgc agtgcttcgc
5161 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
5221 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
5281 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
5341 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca aggtctatat
5401 caccgccgac aagcagaaga acggcatcaa ggtgaacttc aagacccgcc acaacatcga
5461 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
5521 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
5581 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
5641 catggacgag ctgtacaagt aaggtacctt taagaccaat gacttacaag gcagctgtag
5701 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
5761 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
5821 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
5881 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
5941 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
6001 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
6061 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
6121 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
6181 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
6241 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
6301 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
6361 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
6421 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
6481 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
6541 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
6601 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
6661 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
6721 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
6781 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
6841 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
6901 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
6961 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
7021 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
7081 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
7141 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
7201 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
7261 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
7321 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
7381 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
7441 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
7501 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
7561 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
7621 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
7681 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
7741 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
7801 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
7861 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
7921 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
7981 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
8041 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
8101 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
8161 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
8221 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
8281 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
8341 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
8401 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
8461 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
8521 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
8581 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
8641 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
8701 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
8761 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
8821 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
8881 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
8941 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
9001 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
9061 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
9121 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
9181 actaaaggga acaaaagctg gagctgcaag ctt
//
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