| 质粒名称: | FUW-tetO-MCS |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 84008 |
| 存储实验室: | Stefano Piccolo |
|
载体骨架基本信息 |
|
| 载体名称: | FUW |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | 8482bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | AgeI(未破坏) |
| 3' 克隆位点: | NotI (未破坏) |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | FUW-tetO-MCS was a gift from Stefano Piccolo (Addgene plasmid # 84008 ; http://n2t.net/addgene:84008 ; RRID:Addgene_84008) |
| 参考文献部分: | Induction of Expandable Tissue-Specific Stem/Progenitor Cells through Transient Expression of YAP/TAZ. Panciera T, Azzolin L, Fujimura A, Di Biagio D, Frasson C, Bresolin S, Soligo S, Basso G, Bicciato S, Rosato A, Cordenonsi M, Piccolo S. Cell Stem Cell. 2016 Sep 9. pii: S1934-5909(16)30256-9. doi: 10.1016/j.stem.2016.08.009. 10.1016/j.stem.2016.08.009 PubMed 27641305 |
买家导航
FUW-tetO-MCS载体质粒基本信息
FUW-tetO-MCS质粒图谱载体图谱和FUW-tetO-MCS载体序列质粒序列多克隆位点信息
FUW-tetO-MCS质粒载体简介
FUW-tetO-MCS质粒序列载体序列
LOCUS FUW-tetO-MCS 8477 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION doxycycline-inducible lentiviral vector.
ACCESSION .
VERSION .
KEYWORDS FUW-tetO-MCS
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8477)
AUTHORS Panciera T, Azzolin L, Fujimura A, Di Biagio D, Frasson C, Bresolin
S, Soligo S, Basso G, Bicciato S, Rosato A, Cordenonsi M, Piccolo S
TITLE Induction of Expandable Tissue-Specific Stem/Progenitor Cells
through Transient Expression of YAP/TAZ.
JOURNAL Cell Stem Cell. 2016 Sep 9. pii: S1934-5909(16)30256-9. doi:
10.1016/j.stem.2016.08.009.
PUBMED 27641305
REFERENCE 2 (bases 1 to 8477)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8477
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 27..406
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 408..606
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 561..581
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 624..804
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 851..976
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1469..1702
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 2229..2346
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
protein_bind 2399..2669
/label=tetracycline response element
/bound_moiety="tetracycline repressor TetR"
/note="contains seven copies of the tetracycline operator
tetO"
promoter 2702..2740
/label=minimal CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 2737..2761
/label=LNCX
/note="Human CMV promoter, forward primer"
misc_feature 2958..3546
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(3011..3031)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(3429..3440)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(3549..3565)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3550..3566)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 4071..4251
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
primer_bind complement(4277..4294)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 4283..4507
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 4553..4981
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4640..4659)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4850..4871
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(4990..5010)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 4995..5324
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 5175..5310
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 5237..5256
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 5372..5419
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 5438..5812
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 5942..6063
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5979..5998)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 6033..6052
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(6112..6128)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6112..6128)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(6125..6147)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 6136..6152
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6160..6190)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6205..6226
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6343..6360)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6514..7102)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6594..6613)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7273..8133)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7896..7915
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8134..8238)
/gene="bla"
/label=AmpR promoter
primer_bind complement(8313..8332)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
601 aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
661 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
721 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
781 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
841 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
901 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
961 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
1021 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
1081 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
1141 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
1201 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
1261 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
1321 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
1381 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
1441 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
1501 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
1561 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
1621 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
1681 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
1741 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
1801 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
1861 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
1921 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
1981 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
2041 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
2101 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
2161 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
2221 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
2281 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
2341 aattttcggg tttattacag ggacagcaga gatccagttt ggttatcgag tttaccactc
2401 cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa
2461 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
2521 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
2581 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
2641 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagctcggt acccgggtcg
2701 aggtaggcgt gtacggtggg aggcctatat aagcagagct cgtttagtga accgtcagat
2761 cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag
2821 cctccgcggc cccgaattac aggctagcta tcaggatcct acgaggcgcg ccagatagaa
2881 ttctcttggg cctcaagggc catgagttaa ccagttctag atgtgactca tgtcaattcg
2941 atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
3001 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
3061 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
3121 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
3181 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
3241 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
3301 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
3361 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
3421 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
3481 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
3541 ccccgcatcg ataccgtcga cctcgagacc tagaaaaaca tggagcaatc acaagtagca
3601 atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg
3661 gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc
3721 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
3781 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact
3841 acacaccagg gccagggatc agatatccac tgacctttgg atggtgctac aagctagtac
3901 cagttgagca agagaaggta gaagaagcca atgaaggaga gaacacccgc ttgttacacc
3961 ctgtgagcct gcatgggatg gatgacccgg agagagaagt attagagtgg aggtttgaca
4021 gccgcctagc atttcatcac atggcccgag agctgcatcc ggactgtact gggtctctct
4081 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
4141 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
4201 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agggcccgtt
4261 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
4321 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
4381 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
4441 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
4501 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
4561 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
4621 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
4681 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
4741 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
4801 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
4861 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
4921 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
4981 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
5041 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
5101 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
5161 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
5221 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
5281 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
5341 gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat
5401 cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt
5461 tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg
5521 gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct
5581 gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt
5641 gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga
5701 cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct
5761 gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct
5821 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
5881 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
5941 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
6001 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
6061 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
6121 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
6181 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
6241 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
6301 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
6361 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
6421 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
6481 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
6541 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
6601 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
6661 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
6721 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
6781 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
6841 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
6901 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
6961 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
7021 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
7081 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
7141 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
7201 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
7261 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
7321 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
7381 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
7441 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
7501 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
7561 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
7621 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
7681 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
7741 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
7801 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
7861 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
7921 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
7981 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
8041 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
8101 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
8161 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
8221 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg
8281 agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
8341 agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt
8401 taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg
8461 cgttttgcgc tgcttcg
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