| 质粒名称: | pCS Omomyc |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 113164 |
| 存储实验室: | Sergio Nasi |
|
载体骨架基本信息 |
|
| 载体名称: | pCS+ |
| 载体出品公司: | David Turner |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Omomyc |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 300 bp |
| 基因突变: | E57T, E64I, R70Q, R71N |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pCS Omomyc was a gift from Sergio Nasi (Addgene plasmid # 113164 ; http://n2t.net/addgene:113164 ; RRID:Addgene_113164) |
| 参考文献部分: | Design and properties of a Myc derivative that efficiently homodimerizes. Soucek L, Helmer-Citterich M, Sacco A, Jucker R, Cesareni G, Nasi S. Oncogene. 1998 Nov 12;17(19):2463-72. doi: 10.1038/sj.onc.1202199. 10.1038/sj.onc.1202199 PubMed 9824157 |
买家导航
pCSOmomyc载体基本信息
pCSOmomyc载体图谱质粒图谱和多克隆位点信息
pCSOmomyc载体简介
pCSOmomyc载体序列
LOCUS pCS Omomyc 4387 bp ds-DNA circular SYN 07-AUG-2018
DEFINITION Omomyc expression in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS pCS Omomyc
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4387)
AUTHORS Soucek L, Helmer-Citterich M, Sacco A, Jucker R, Cesareni G, Nasi S
TITLE Design and properties of a Myc derivative that efficiently
homodimerizes.
JOURNAL Oncogene. 1998 Nov 12;17(19):2463-72. doi: 10.1038/sj.onc.1202199.
PUBMED 9824157
REFERENCE 2 (bases 1 to 4387)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 7, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4387
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 20..997
/label=CMV IE94 promoter
/note="enhancer/promoter region of simian cytomegalovirus
major immediate early transcription unit IE94"
promoter 1032..1050
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind 1032..1049
/label=SP6
/note="SP6 promoter, forward primer"
regulatory 1075..1084
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
promoter complement(1414..1431)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
polyA_signal 1436..1570
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1460..1479)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 1514..1533
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(1683..1703)
/label=T3
/note="T3 promoter, forward primer"
promoter complement(1683..1701)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(1722..1738)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(1722..1738)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(1735..1757)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 1746..1762
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(1770..1800)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 1815..1836
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(1953..1970)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(2124..2712)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(2204..2223)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(2883..3743)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 3506..3525
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(3744..3848)
/gene="bla"
/label=AmpR promoter
rep_origin complement(3874..4329)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4011..4032)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 4223..4242
/label=F1ori-R
/note="F1 origin, reverse primer"
ORIGIN
1 gctattacgc cagtcgaccg ccaattcaat atggcgtata tggactcatg ccaattcaat
61 atggtggatc tggacctgtg ccaattcaat atggcgtata tggactcgtg ccaattcaat
121 atggtggatc tggaccccag ccaattcaat atggcggact tggcaccatg ccaattcaat
181 atggcggacc tggcactgtg ccaactgggg aggggtctac ttggcacggt gccaagtttg
241 aggaggggtc ttggccctgt gccaagtccg ccatattgaa ttggcatggt gccaataatg
301 gcggccatat tggctatatg ccaggatcaa tatataggca atatccaata tggccctatg
361 ccaatatggc tattggccag gttcaatact atgtattggc cctatgccat atagtattcc
421 atatatgggt tttcctattg acgtagatag cccctcccaa tgggcggtcc catataccat
481 atatggggct tcctaatacc gcccatagcc actcccccat tgacgtcaat ggtctctata
541 tatggtcttt cctattgacg tcatatgggc ggtcctattg acgtatatgg cgcctccccc
601 attgacgtca attacggtaa atggcccgcc tggctcaatg cccattgacg tcaataggac
661 cacccaccat tgacgtcaat gggatggctc attgcccatt catatccgtt ctcacgcccc
721 ctattgacgt caatgacggt aaatggccca cttggcagta catcaatatc tattaatagt
781 aacttggcaa gtacattact attggaagta cgccagggta cattggcagt actcccattg
841 acgtcaatgg cggtaaatgg cccgcgatgg ctgccaagta catccccatt gacgtcaatg
901 gggaggggca atgacgcaaa tgggcgttcc attgacgtaa atgggcggta ggcgtgccta
961 atgggaggtc tatataagca atgctcgttt agggaaccgc cattctgcct ggggacgtcg
1021 gagcaagctt gatttaggtg acactataga cttgttcttt ttgcaggatc tgccgccacc
1081 atggggatcc catcgatgtc gaccgaggag aatgtcaaga ggcgaacaca caacgtcttg
1141 gagcgccaga ggaggaacga gctaaaacgg agcttttttg ccctgcgtga ccagatcccg
1201 gagttggaaa acaatgaaaa ggcccccaag gtagttatcc ttaaaaaagc cacagcatac
1261 atcctgtccg tccaagcaga gacgcaaaag ctcatttctg aaatcgactt gttgcggaaa
1321 caaaacgaac agttgaaaca caaacttgaa cagctacgga actcttgtgc gtaagggatc
1381 cccgggaatt caaggcctct cgagcctcta gaactatagt gagtcgtatt acgtagatcc
1441 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
1501 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
1561 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
1621 ggaggttttt taattcgcgg ccgcggcgcc aatgcattgg gcccggtacc cagcttttgt
1681 tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg
1741 tgaaattgtt atccgctcac aattccacac aacatacgag ccgggagcat aaagtgtaaa
1801 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
1861 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
1921 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
1981 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
2041 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
2101 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
2161 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
2221 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
2281 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
2341 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
2401 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
2461 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
2521 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
2581 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
2641 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
2701 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
2761 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
2821 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
2881 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
2941 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
3001 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
3061 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
3121 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
3181 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
3241 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
3301 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
3361 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
3421 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
3481 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
3541 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
3601 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
3661 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
3721 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
3781 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
3841 gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa
3901 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
3961 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
4021 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
4081 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
4141 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
4201 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
4261 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
4321 agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
4381 cctcttc
//
