pDGM6

价格:1200元

联系方式:I47-825O-882O

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载体基本信息

质粒名称: pDGM6 
出品公司: 爱迪基因
目录编号: 110660
存储实验室: David Russell

载体骨架基本信息
载体名称: pDG
载体出品公司: --
载体抗性: 氨苄青霉素
空载体大小: 21845 bp
完整质粒大小: 21842 bp
载体修饰: 包装/辅助质粒pDGM6,包括血清型6衣壳阅读框,用编码SwaI和ClaI末端限制性位点的寡核苷酸引物5′-atttaaatcagtatggctgccgatggt-3′和5′-atcgatgctatggcatgagataa-3′对AAV 6型血清克隆的衣壳基因进行PCR扩增,并将得到的DNA片段插入相同酶切的质粒pDG中。
载体类型: AAV
筛选标记: --
高拷贝/低拷贝: 高拷贝
生长条件: 37 ℃

插入基因信息
插入基因名称: AAV6 cap genes, AAV2 rep genes, adenovirus helper genes
物种来源: adenovirus, adeno-associated virus
插入大小: --
基因突变: --
GenBank编号 --
启动子 --
标签/融合蛋白: --
克隆方法: 限制性内切酶
5' 克隆位点: SwaI(未破坏)
3' 克隆位点: ClaI(未破坏)
5' 测序引物 --
3' 测序引物 --

文献引用方法
材料和方法部分: pDGM6 was a gift from David Russell (Addgene plasmid # 110660 ; http://n2t.net/addgene:110660 ; RRID:Addgene_110660)
参考文献部分: Systemic delivery of genes to striated muscles using adeno-associated viral vectors. Gregorevic P, Blankinship MJ, Allen JM, Crawford RW, Meuse L, Miller DG, Russell DW, Chamberlain JS. Nat Med. 2004 Aug;10(8):828-34. doi: 10.1038/nm1085. Epub 2004 Jul 25. 10.1038/nm1085 PubMed 15273747

载体质粒图谱和多克隆位点信息

pDGM6质粒图谱

载体简介

载体序列

LOCUS       pDGM6               21829 bp ds-DNA     circular SYN 06-AUG-2018
DEFINITION  Adeno-Associated Virus (AAV) serotype 6; contains the AAV6 cap 
            genes, AAV2 rep genes, and adenovirus helper genes. Good for in vivo
            transduction of muscle, lung, liver and other tissues..
ACCESSION   .
VERSION     .
KEYWORDS    pDGM6
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 21829)
  AUTHORS   Gregorevic P, Blankinship MJ, Allen JM, Crawford RW, Meuse L, Miller
            DG, Russell DW, Chamberlain JS
  TITLE     Systemic delivery of genes to striated muscles using 
            adeno-associated viral vectors.
  JOURNAL   Nat Med. 2004 Aug;10(8):828-34. doi: 10.1038/nm1085. Epub 2004 Jul 
            25.
  PUBMED    15273747
REFERENCE   2  (bases 1 to 21829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..21829
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   21..123
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     misc_signal     211..361
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     primer_bind     483..499
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     483..499
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     regulatory      7501..7510
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     repeat_region   19726..19828
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     rep_origin      complement(20019..20607)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(20099..20118)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(20759..21619)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     21382..21401
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(21620..21724)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     21792..21810
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ggatccgaat tcttaattaa catcatcaat aatatacctt attttggatt gaagccaata
       61 tgataatgag ggggtggagt ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg
      121 tagtagtgtg gcggaagtgt gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg
      181 tggcaaaagt gacgtttttg gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg
      241 gttttaggcg gatgttgtag taaatttggg cgtaaccgag taagatttgg ccattttcgc
      301 gggaaaactg aataagagga agtgaaatct gaataatttt gtgttactca tagcgcgtaa
      361 tatttgtcta gggccgcggg gactttgacc gtttacgtgg agactcgccc aggtgttttt
      421 ctcaggtgtt ttccgcgttc cgggtcaaag ttggcgtttt attattatag tcagggggat
      481 cctctagaac tagtggatcc gtccctagaa gtaaaaaagg gaaaaaagag tgtgtttgtc
      541 aaaataggag acaggtggtg gcaaccaggg acttataggg gaccttacat ctacagacca
      601 acagatgccc ccttaccata tacaggaaga tatgacttaa attgggatag gtgggtcaca
      661 atcaacggct ataaagtgtt atacagatcc ctcccctttc gtgaaagact cgccagagct
      721 agacctcctt ggtgtatgct aactgagaaa gagaaagacg acatgaaaca acaggtacat
      781 gattatattt atctaggaac aggaatgcac ttttggggaa aggttttcca taccaaggaa
      841 ggggcagtgg ctggactgat agaacattat tctgcaaaaa cttatggtat gagttattat
      901 gattagcctt tatttgccca accttgcggt tcccagggtt taaataagtt tatggttaca
      961 aactgttctt aaaacaagga tgtgagacaa gtggtttcct gagttggttt ggtatcaaat
     1021 gttctgatct gagctcttag tgttctattt tcctatgttc ttttggaatc tatccaagtc
     1081 ttatgtaaat gcttatgtaa accataatat aaaagagtgc tgattttttg agtaaacttg
     1141 caacagtcct aacattcttc tctcgtgtgt ttgtgtctgt tcgccatccc gtctccgctc
     1201 gtcacttatc cttcactttt cagagggtcc ccccgcagat cccggtcacc ctcaggtcgg
     1261 gacctgcaga agacgcccga gtgagcacgc agggtctcca ttttgaagcg ggaggtttga
     1321 acgcgcagcc gccatgccgg ggttttacga gattgtgatt aaggtcccca gcgaccttga
     1381 cgagcatctg cccggcattt ctgacagctt tgtgaactgg gtggccgaga aggaatggga
     1441 gttgccgcca gattctgaca tggatctgaa tctgattgag caggcacccc tgaccgtggc
     1501 cgagaagctg cagcgcgact ttctgacgga atggcgccgt gtgagtaagg ccccggaggc
     1561 ccttttcttt gtgcaatttg agaagggaga gagctacttc cacatgcacg tgctcgtgga
     1621 aaccaccggg gtgaaatcca tggttttggg acgtttcctg agtcagattc gcgaaaaact
     1681 gattcagaga atttaccgcg ggatcgagcc gactttgcca aactggttcg cggtcacaaa
     1741 gaccagaaat ggcgccggag gcgggaacaa ggtggtggat gagtgctaca tccccaatta
     1801 cttgctcccc aaaacccagc ctgagctcca gtgggcgtgg actaatatgg aacagtattt
     1861 aagcgcctgt ttgaatctca cggagcgtaa acggttggtg gcgcagcatc tgacgcacgt
     1921 gtcgcagacg caggagcaga acaaagagaa tcagaatccc aattctgatg cgccggtgat
     1981 cagatcaaaa acttcagcca ggtacatgga gctggtcggg tggctcgtgg acaaggggat
     2041 tacctcggag aagcagtgga tccaggagga ccaggcctca tacatctcct tcaatgcggc
     2101 ctccaactcg cggtcccaaa tcaaggctgc cttggacaat gcgggaaaga ttatgagcct
     2161 gactaaaacc gcccccgact acctggtggg ccagcagccc gtggaggaca tttccagcaa
     2221 tcggatttat aaaattttgg aactaaacgg gtacgatccc caatatgcgg cttccgtctt
     2281 tctgggatgg gccacgaaaa agttcggcaa gaggaacacc atctggctgt ttgggcctgc
     2341 aactaccggg aagaccaaca tcgcggaggc catagcccac actgtgccct tctacgggtg
     2401 cgtaaactgg accaatgaga actttccctt caacgactgt gtcgacaaga tggtgatctg
     2461 gtgggaggag gggaagatga ccgccaaggt cgtggagtcg gccaaagcca ttctcggagg
     2521 aagcaaggtg cgcgtggacc agaaatgcaa gtcctcggcc cagatagacc cgactcccgt
     2581 gatcgtcacc tccaacacca acatgtgcgc cgtgattgac gggaactcaa cgaccttcga
     2641 acaccagcag ccgttgcaag accggatgtt caaatttgaa ctcacccgcc gtctggatca
     2701 tgactttggg aaggtcacca agcaggaagt caaagacttt ttccggtggg caaaggatca
     2761 cgtggttgag gtggagcatg aattctacgt caaaaagggt ggagccaaga aaagacccgc
     2821 ccccagtgac gcagatataa gtgagcccaa acgggtgcgc gagtcagttg cgcagccatc
     2881 gacgtcagac gcggaagctt cgatcaacta cgcagacagg taccaaaaca aatgttctcg
     2941 tcacgtgggc atgaatctga tgctgtttcc ctgcagacaa tgcgagagaa tgaatcagaa
     3001 ttcaaatatc tgcttcactc acggacagaa agactgttta gagtgctttc ccgtgtcaga
     3061 atctcaaccc gtttctgtcg tcaaaaaggc gtatcagaaa ctgtgctaca ttcatcatat
     3121 catgggaaag gtgccagacg cttgcactgc ctgcgatctg gtcaatgtgg atttggatga
     3181 ctgcatcttt gaacaataaa tgatttaaat caggtatggc tgccgatggt tatcttccag
     3241 attggctcga ggacaacctc tctgagggca ttcgcgagtg gtgggacttg aaacctggag
     3301 ccccgaaacc caaagccaac cagcaaaagc aggacgacgg ccggggtctg gtgcttcctg
     3361 gctacaagta cctcggaccc ttcaacggac tcgacaaggg ggagcccgtc aacgcggcgg
     3421 atgcagcggc cctcgagcac gacaaggcct acgaccagca gctcaaagcg ggtgacaatc
     3481 cgtacctgcg gtataaccac gccgacgccg agtttcagga gcgtctgcaa gaagatacgt
     3541 cttttggggg caacctcggg cgagcagtct tccaggccaa gaagagggtt ctcgaacctt
     3601 ttggtctggt tgaggaaggt gctaagacgg ctcctggaaa gaaacgtccg gtagagcagt
     3661 cgccacaaga gccagactcc tcctcgggca ttggcaagac aggccagcag cccgctaaaa
     3721 agagactcaa ttttggtcag actggcgact cagagtcagt ccccgaccca caacctctcg
     3781 gagaacctcc agcaaccccc gctgctgtgg gacctactac aatggcttca ggcggtggcg
     3841 caccaatggc agacaataac gaaggcgccg acggagtggg taatgcctca ggaaattggc
     3901 attgcgattc cacatggctg ggcgacagag tcatcaccac cagcacccga acatgggcct
     3961 tgcccaccta taacaaccac ctctacaagc aaatctccag tgcttcaacg ggggccagca
     4021 acgacaacca ctacttcggc tacagcaccc cctgggggta ttttgatttc aacagattcc
     4081 actgccattt ctcaccacgt gactggcagc gactcatcaa caacaattgg ggattccggc
     4141 ccaagagact caacttcaag ctcttcaaca tccaagtcaa ggaggtcacg acgaatgatg
     4201 gcgtcacgac catcgctaat aaccttacca gcacggttca agtcttctcg gactcggagt
     4261 accagttgcc gtacgtcctc ggctctgcgc accagggctg cctccctccg ttcccggcgg
     4321 acgtgttcat gattccgcag tacggctacc taacgctcaa caatggcagc caggcagtgg
     4381 gacggtcatc cttttactgc ctggaatatt tcccatcgca gatgctgaga acgggcaata
     4441 actttacctt cagctacacc ttcgaggacg tgcctttcca cagcagctac gcgcacagcc
     4501 agagcctgga ccggctgatg aatcctctca tcgaccagta cctgtattac ctgaacagaa
     4561 ctcagaatca gtccggaagt gcccaaaaca aggacttgct gtttagccgg gggtctccag
     4621 ctggcatgtc tgttcagccc aaaaactggc tacctggacc ctgttaccgg cagcagcgcg
     4681 tttctaaaac aaaaacagac aacaacaaca gcaactttac ctggactggt gcttcaaaat
     4741 ataaccttaa tgggcgtgaa tctataatca accctggcac tgctatggcc tcacacaaag
     4801 acgacaaaga caagttcttt cccatgagcg gtgtcatgat ttttggaaag gagagcgccg
     4861 gagcttcaaa cactgcattg gacaatgtca tgatcacaga cgaagaggaa atcaaagcca
     4921 ctaaccccgt ggccaccgaa agatttggga ctgtggcagt caatctccag agcagcagca
     4981 cagaccctgc gaccggagat gtgcatgtta tgggagcctt acctggaatg gtgtggcaag
     5041 acagagacgt atacctgcag ggtcctattt gggccaaaat tcctcacacg gatggacact
     5101 ttcacccgtc tcctctcatg ggcggctttg gacttaagca cccgcctcct cagatcctca
     5161 tcaaaaacac gcctgttcct gcgaatcctc cggcagagtt ttcggctaca aagtttgctt
     5221 cattcatcac ccagtattcc acaggacaag tgagcgtgga gattgaatgg gagctgcaga
     5281 aagaaaacag caaacgctgg aatcccgaag tgcagtatac atctaactat gcaaaatctg
     5341 ccaacgttga tttcactgtg gacaacaatg gactttatac tgagcctcgc cccattggca
     5401 cccgttacct cacccgtccc ctgtaattgt ctgttaatca ataaaccggt taattcgtgt
     5461 cagttgaact ttggtctcat gtcgttatta tcttatctgg tcaccatagc aatcgatgca
     5521 tgtccttggg tccggcctgc tgaatgcgca ggcggtcggc catgccccag gcttcgtttt
     5581 gacatcggcg caggtctttg tagtagtctt gcatgagcct ttctaccggc acttcttctt
     5641 ctccttcctc ttgtcctgca tctcttgcat ctatcgctgc ggcggcggcg gagtttggcc
     5701 gtaggtggcg ccctcttcct cccatcgtgt gaccccgaag cccctcatcg gctgaagcag
     5761 ggctaggtcg gcgacaacgc gctcggctaa tatggcctgc tgcacctgcg tgagggtaga
     5821 ctggaagtca tccatgtcca caaagcggtg gtatgcgccc gtgttgatgg tgtaagtgca
     5881 gttggccata acggaccagt taacggtctg gtgacccggc tgcgagagct cggtgtacct
     5941 gagacgcgag taagccctcg agtcaaatac gtagtcgtta caagtccgca ccaggtactg
     6001 gtatcccacc aaaaagtgcg gcggcggctg gcggtagagg ggccagcgta gggtggccgg
     6061 ggctccgggg gcgagatctt ccaacataag gcgatgatat ccgtagatgt acctggacat
     6121 ccaggtgatg ccggcggcgg tggtggaggc gcgcggaaag tcgcggacgc ggttccagat
     6181 gttgcgcagc ggcaaaaagt gctccatggt cgggacgctc tggccggtca ggcgcgcgca
     6241 atcgttgacg ctctagaccg tgcaaaagga gagcctgtaa gcgggcactc ttccgtggtc
     6301 tggtggataa attcgcaagg gtatcatggc ggacgaccgg ggttcgagcc ccgtatccgg
     6361 ccgtccgccg tgatccatgc ggttaccgcc cgcgtgtcga acccaggtgt gcgacgtcag
     6421 acaacggggg agtgctcctt ttggcttcct tccaggcgcg gcggctgctg cgctagcttt
     6481 tttggccact ggccgcgcgc agcgtaagcg gttaggctgg aaagcgaaag cattaagtgg
     6541 ctcgctccct gtagccggag ggttattttc caagggttga gtcgcgggac ccccggttcg
     6601 agtctcggac cggccggact gcggcgaacg ggggtttgtc tccccgtcat gcaagacccc
     6661 gcttgcaaat tcctccggaa acagggacga gccccttttt tgcttttccc agatgcatcc
     6721 ggtgctgcgg cagatgcgcc cccctcctca gcagcggcaa gagcaagagc agcggcagac
     6781 atgcagggca ccctcccctc ctcctaccgc gtcaggaggg gcgacatccg cggttgacgc
     6841 ggcagcagat ggtgattacg aacccccgcg gcgccgggcc cggcactacc tggacttgga
     6901 ggagggcgag ggcctggcgc ggctaggagc gccctctcct gagcggcacc caagggtgca
     6961 gctgaagcgt gatacgcgtg aggcgtacgt gccgcggcag aacctgtttc gcgaccgcga
     7021 gggagaggag cccgaggaga tgcgggatcg aaagttccac gcagggcgcg agctgcggca
     7081 tggcctgaat cgcgagcggt tgctgcgcga ggaggacttt gagcccgacg cgcgaaccgg
     7141 gattagtccc gcgcgcgcac acgtggcggc cgccgacctg gtaaccgcat acgagcagac
     7201 ggtgaaccag ggcgatcgca ccctttggcg catcccattc tccagtaact ttatgtccat
     7261 gggcgcactc acagacctgg gccaaaacct tctctacgcc aactccgccc acgcgctaga
     7321 catgactttt gaggtggatc ccatggacga gcccaccctt ctttatgttt tgtttgaagt
     7381 ctttgacgtg gtccgtgtgc accagccgca ccgcggcgtc atcgaaaccg tgtacctgcg
     7441 cacgcccttc tcggccggca acgccacaac ataaagaagc aagcaacatc aacaacagct
     7501 gccgccatgg gctccagtga gcaggaactg aaagccattg tcaaagatct tggttgtggg
     7561 ccatattttt tgggcaccta tgacaagcgc tttccaggct ttgtttctcc acacaagctc
     7621 gcctgcgcca tagtcaatac ggccggtcgc gagactgggg gcgtacactg gatggccttt
     7681 gcctggaacc cgcactcaaa aacatgctac ctctttgagc cctttggctt ttctgaccag
     7741 cgactcaagc aggtttacca gtttgagtac gagtcactcc tgcgccgtag cgccattgct
     7801 tcttcccccg accgctgtat aacgctggaa aagtccaccc aaagcgtaca ggggcccaac
     7861 tcggccgcct gtggactatt ctgctgcatg tttctccacg cctttgccaa ctggccccaa
     7921 actcccatgg atcacaaccc caccatgaac cttattaccg gggtacccaa ctccatgctc
     7981 aacagtcccc aggtacagcc caccctgcgt cgcaaccagg aacagctcta cagcttcctg
     8041 gagcgccact cgccctactt ccgcagccac agtgcgcaga ttaggagcgc cacttctttt
     8101 tgtcacttga aaaacatgta aaaataatgt actagagaca ctttcaataa aggcaaatgc
     8161 ttttatttgt acactctcgg gtgattattt acccccaccc ttgccgtctg cgccgtttaa
     8221 aaatcaaagg ggttctgccg cgcatcgcta tgcgccactg gcagggacac gttgcgatac
     8281 tggtgtttag tgctccactt aaactcaggc acaaccatcc gcggcagctc ggtgaagttt
     8341 tcactccaca ggctgcgcac catcaccaac gcgtttagca ggtcgggcgc cgatatcttg
     8401 aagtcgcagt tggggcctcc gccctgcgcg cgcgagttgc gatacacagg gttgcagcac
     8461 tggaacacta tcagcgccgg gtggtgcacg ctggccagca cgctcttgtc ggagatcaga
     8521 tccgcgtcca ggtcctccgc gttgctcagg gcgaacggag tcaactttgg tagctgcctt
     8581 cccaaaaagg gcgcgtgccc aggctttgag ttgcactcgc accgtagtgg catcaaaagg
     8641 tgaccgtgcc cggtctgggc gttaggatac agcgcctgca taaaagcctt gatctgctta
     8701 aaagccacct gagcctttgc gccttcagag aagaacatgc cgcaagactt gccggaaaac
     8761 tgattggccg gacaggccgc gtcgtgcacg cagcaccttg cgtcggtgtt ggagatctgc
     8821 accacatttc ggccccaccg gttcttcacg atcttggcct tgctagactg ctccttcagc
     8881 gcgcgctgcc cgttttcgct cgtcacatcc atttcaatca cgtgctcctt atttatcata
     8941 atgcttccgt gtagacactt aagctcgcct tcgatctcag cgcagcggtg cagccacaac
     9001 gcgcagcccg tgggctcgtg atgcttgtag gtcacctctg caaacgactg caggtacgcc
     9061 tgcaggaatc gccccatcat cgtcacaaag gtcttgttgc tggtgaaggt cagctgcaac
     9121 ccgcggtgct cctcgttcag ccaggtcttg catacggccg ccagagcttc cacttggtca
     9181 ggcagtagtt tgaagttcgc ctttagatcg ttatccacgt ggtacttgtc catcagcgcg
     9241 cgcgcagcct ccatgccctt ctcccacgca gacacgatcg gcacactcag cgggttcatc
     9301 accgtaattt cactttccgc ttcgctgggc tcttcctctt cctcttgcgt ccgcatacca
     9361 cgcgccactg ggtcgtcttc attcagccgc cgcactgtgc gcttacctcc tttgccatgc
     9421 ttgattagca ccggtgggtt gctgaaaccc accatttgta gcgccacatc ttctctttct
     9481 tcctcgctgt ccacgattac ctctggtgat ggcgggcgct cgggcttggg agaagggcgc
     9541 ttctttttct tcttgggcgc aatggccaaa tccgccgccg aggtcgatgg ccgcgggctg
     9601 ggtgtgcgcg gcaccagcgc gtcttgtgat gagtcttcct cgtcctcgga ctcgatacgc
     9661 cgcctcatcc gcttttttgg gggcgcccgg ggaggcggcg gcgacgggga cggggacgac
     9721 acgtcctcca tggttggggg acgtcgcgcc gcaccgcgtc cgcgctcggg ggtggtttcg
     9781 cgctgctcct cttcccgact ggccatttcc ttctcctata ggcagaaaaa gatcatggag
     9841 tcagtcgaga agaaggacag cctaaccgcc ccctctgagt tcgccaccac cgcctccacc
     9901 gatgccgcca acgcgcctac caccttcccc gtcgaggcac ccccgcttga ggaggaggaa
     9961 gtgattatcg agcaggaccc aggttttgta agcgaagacg acgaggaccg ctcagtacca
    10021 acagaggata aaaagcaaga ccaggacaac gcagaggcaa acgaggaaca agtcgggcgg
    10081 ggggacgaaa ggcatggcga ctacctagat gtgggagacg acgtgctgtt gaagcatctg
    10141 cagcgccagt gcgccattat ctgcgacgcg ttgcaagagc gcagcgatgt gcccctcgcc
    10201 atagcggatg tcagccttgc ctacgaacgc cacctattct caccgcgcgt accccccaaa
    10261 cgccaagaaa acggcacatg cgagcccaac ccgcgcctca acttctaccc cgtatttgcc
    10321 gtgccagagg tgcttgccac ctatcacatc tttttccaaa actgcaagat acccctatcc
    10381 tgccgtgcca accgcagccg agcggacaag cagctggcct tgcggcaggg cgctgtcata
    10441 cctgatatcg cctcgctcaa cgaagtgcca aaaatctttg agggtcttgg acgcgacgag
    10501 aagcgcgcgg caaacgctct gcaacaggaa aacagcgaaa atgaaagtca ctctggagtg
    10561 ttggtggaac tcgagggtga caacgcgcgc ctagccgtac taaaacgcag catcgaggtc
    10621 acccactttg cctacccggc acttaaccta ccccccaagg tcatgagcac agtcatgagt
    10681 gagctgatcg tgcgccgtgc gcagcccctg gagagggatg caaatttgca agaacaaaca
    10741 gaggagggcc tacccgcagt tggcgacgag cagctagcgc gctggcttca aacgcgcgag
    10801 cctgccgact tggaggagcg acgcaaacta atgatggccg cagtgctcgt taccgtggag
    10861 cttgagtgca tgcagcggtt ctttgctgac ccggagatgc agcgcaagct agaggaaaca
    10921 ttgcactaca cctttcgaca gggctacgta cgccaggcct gcaagatctc caacgtggag
    10981 ctctgcaacc tggtctccta ccttggaatt ttgcacgaaa accgccttgg gcaaaacgtg
    11041 cttcattcca cgctcaaggg cgaggcgcgc cgcgactacg tccgcgactg cgtttactta
    11101 tttctatgct acacctggca gacggccatg ggcgtttggc agcagtgctt ggaggagtgc
    11161 aacctcaagg agctgcagaa actgctaaag caaaacttga aggacctatg gacggccttc
    11221 aacgagcgct ccgtggccgc gcacctggcg gacatcattt tccccgaacg cctgcttaaa
    11281 accctgcaac agggtctgcc agacttcacc agtcaaagca tgttgcagaa ctttaggaac
    11341 tttatcctag agcgctcagg aatcttgccc gccacctgct gtgcacttcc tagcgacttt
    11401 gtgcccatta agtaccgcga atgccctccg ccgctttggg gccactgcta ccttctgcag
    11461 ctagccaact accttgccta ccactctgac ataatggaag acgtgagcgg tgacggtcta
    11521 ctggagtgtc actgtcgctg caacctatgc accccgcacc gctccctggt ttgcaattcg
    11581 cagctgctta acgaaagtca aattatcggt acctttgagc tgcagggtcc ctcgcctgac
    11641 gaaaagtccg cggctccggg gttgaaactc actccggggc tgtggacgtc ggcttacctt
    11701 cgcaaatttg tacctgagga ctaccacgcc cacgagatta ggttctacga agaccaatcc
    11761 cgcccgccta atgcggagct taccgcctgc gtcattaccc agggccacat tcttggccaa
    11821 ttgcaagcca tcaacaaagc ccgccaagag tttctgctac gaaagggacg gggggtttac
    11881 ttggaccccc agtccggcga ggagctcaac ccaatccccc cgccgccgca gccctatcag
    11941 cagcagccgc gggcccttgc ttcccaggat ggcacccaaa aagaagctgc agctgccgcc
    12001 gccacccacg gacgaggagg aatactggga cagtcaggca gaggaggttt tggacgagga
    12061 ggaggaggac atgatggaag actgggagag cctagacgag gaagcttccg aggtcgaaga
    12121 ggtgtcagac gaaacaccgt caccctcggt cgcattcccc tcgccggcgc cccagaaatc
    12181 ggcaaccggt tccagcatgg ctacaacctc cgctcctcag gcgccgccgg cactgcccgt
    12241 tcgccgaccc aaccgtagat gggacaccac tggaaccagg gccggtaagt ccaagcagcc
    12301 gccgccgtta gcccaagagc aacaacagcg ccaaggctac cgctcatggc gcgggcacaa
    12361 gaacgccata gttgcttgct tgcaagactg tgggggcaac atctccttcg cccgccgctt
    12421 tcttctctac catcacggcg tggccttccc ccgtaacatc ctgcattact accgtcatct
    12481 ctacagccca tactgcaccg gcggcagcgg cagcaacagc agcggccaca cagaagcaaa
    12541 ggcgaccgga tagcaagact ctgacaaagc ccaagaaatc cacagcggcg gcagcagcag
    12601 gaggaggagc gctgcgtctg gcgcccaacg aacccgtatc gacccgcgag cttagaaaca
    12661 ggatttttcc cactctgtat gctatatttc aacagagcag gggccaagaa caagagctga
    12721 aaataaaaaa caggtctctg cgatccctca cccgcagctg cctgtatcac aaaagcgaag
    12781 atcagcttcg gcgcacgctg gaagacgcgg aggctctctt cagtaaatac tgcgcgctga
    12841 ctcttaagga ctagtttcgc gccctttctc aaatttaagc gcgaaaacta cgtcatctcc
    12901 agcggccaca cccggcgcca gcacctgttg tcagcgccat tatgagcaag gaaattccca
    12961 cgccctacat gtggagttac cagccacaaa tgggacttgc ggctggagct gcccaagact
    13021 actcaacccg aataaactac atgagcgcgg gaccccacat gatatcccgg gtcaacggaa
    13081 tacgcgccca ccgaaaccga attctcctgg aacaggcggc tattaccacc acacctcgta
    13141 ataaccttaa tccccgtagt tggcccgctg ccctggtgta ccaggaaagt cctgctccca
    13201 ccactgtggt acttcccaga gacgcccagg ccgaagttca gatgactaac tcaggggcgc
    13261 agcttgcggg cggctttcgt cacagggtgc ggtcgcccgg gcagggtata actcacctga
    13321 caatcagagg gcgaggtatt cagctcaacg acgagtcggt gagctcctcg cttggtctcc
    13381 gtccggacgg gacatttcag atcggcggcg ccggccgctc ttcattcacg cctcgtcagg
    13441 caatcctaac tctgcagacc tcgtcctctg agccgcgctc tggaggcatt ggaactctgc
    13501 aatttattga ggagtttgtg ccatcggtct actttaaccc cttctcggga cctcccggcc
    13561 actatccgga tcaatttatt cctaactttg acgcggtaaa ggactcggcg gacggctacg
    13621 actgaatgtt aagtggagag gcagagcaac tgcgcctgaa acacctggtc cactgtcgcc
    13681 gccacaagtg ctttgcccgc gactccggtg agttttgcta ctttgaattg cccgaggatc
    13741 atatcgaggg cccggcgcac ggcgtccggc ttaccgccca gggagagctt gcccgtagcc
    13801 tgattcggga gtttacccag cgccccctgc tagttgagcg ggacagggga ccctgtgttc
    13861 tcactgtgat ttgcaactgt cctaaccctg gattacatca agatctttgt tgccatctct
    13921 gtgctgagta taataaatac agaaattaaa atatactggg gctcctatcg ccatcctgta
    13981 aacgccaccg tcttcacccg cccaagcaaa ccaaggcgaa ccttacctgg tacttttaac
    14041 atctctccct ctgtgattta caacagtttc aacccagacg gagtgagtct acgagagaac
    14101 ctctccgagc tcagctactc catcagaaaa aacaccaccc tccttacctg ccgggaacgt
    14161 acgagtgcgt caccggccgc tgcaccacac ctaccgcctg accgtaaacc agactttttc
    14221 cggacagacc tcaataactc tgtttaccag aacaggaggt gagcttagaa aacccttagg
    14281 gtattaggcc aaaggcgcag ctactgtggg gtttatgaac aattcaagca actctacggg
    14341 ctattctaat tcaggtttct ctagaaatgg acggaattat tacagagcag cgcctgctag
    14401 aaagacgcag ggcagcggcc gagcaacagc gcatgaatca agagctccaa gacatggtta
    14461 acttgcacca gtgcaaaagg ggtatctttt gtctggtaaa gcaggccaaa gtcacctacg
    14521 acagtaatac caccggacac cgccttagct acaagttgcc aaccaagcgt cagaaattgg
    14581 tggtcatggt gggagaaaag cccattacca taactcagca ctcggtagaa accgaaggct
    14641 gcattcactc accttgtcaa ggacctgagg atctctgcac ccttattaag accctgtgcg
    14701 gtctcaaaga tcttattccc tttaactaat aaaaaaaaat aataaagcat cacttactta
    14761 aaatcagtta gcaaatttct gtccagttta ttcagcagca cctccttgcc ctcctcccag
    14821 ctctggtatt gcagcttcct cctggctgca aactttctcc acaatctaaa tggaatgtca
    14881 gtttcctcct gttcctgtcc atccgcaccc actatcttca tgttgttgca gatgaagcgc
    14941 gcaagaccgt ctgaagatac cttcaacccc gtgtatccat atgacacgga aaccggtcct
    15001 ccaactgtgc cttttcttac tcctcccttt gtatccccca atgggtttca agagagtccc
    15061 cctggggtac tctctttgcg cctatccgaa cctctagtta cctccaatgg catgcttgcg
    15121 ctcaaaatgg gcaacggcct ctctctggac gaggccggca accttacctc ccaaaatgta
    15181 accactgtga gcccacctct caaaaaaacc aagtcaaaca taaacctgga aatatctgca
    15241 cccctcacag ttacctcaga agccctaact gtggctgccg ccgcacctct aatggtcgcg
    15301 ggcaacacac tcaccatgca atcacaggcc ccgctaaccg tgcacgactc caaacttagc
    15361 attgccaccc aaggacccct cacagtgtca gaaggaaagc tagccctgca aacatcaggc
    15421 cccctcacca ccaccgatag cagtaccctt actatcactg cctcaccccc tctaactact
    15481 gccactggta gcttgggcat tgacttgaaa gagcccattt atacacaaaa tggaaaacta
    15541 ggactaaagt acggggctcc tttgcatgta acagacgacc taaacacttt gaccgtagca
    15601 actggtccag gtgtgactat taataatact tccttgcaaa ctaaagttac tggagccttg
    15661 ggttttgatt cacaaggcaa tatgcaactt aatgtagcag gaggactaag gattgattct
    15721 caaaacagac gccttatact tgatgttagt tatccgtttg atgctcaaaa ccaactaaat
    15781 ctaagactag gacagggccc tctttttata aactcagccc acaacttgga tattaactac
    15841 aacaaaggcc tttacttgtt tacagcttca aacaattcca aaaagcttga ggttaaccta
    15901 agcactgcca aggggttgat gtttgacgct acagccatag ccattaatgc aggagatggg
    15961 cttgaatttg gttcacctaa tgcaccaaac acaaatcccc tcaaaacaaa aattggccat
    16021 ggcctagaat ttgattcaaa caaggctatg gttcctaaac taggaactgg ccttagtttt
    16081 gacagcacag gtgccattac agtaggaaac aaaaataatg ataagctaac tttgtggacc
    16141 acaccagctc catctcctaa ctgtagacta aatgcagaga aagatgctaa actcactttg
    16201 gtcttaacaa aatgtggcag tcaaatactt gctacagttt cagttttggc tgttaaaggc
    16261 agtttggctc caatatctgg aacagttcaa agtgctcatc ttattataag atttgacgaa
    16321 aatggagtgc tactaaacaa ttccttcctg gacccagaat attggaactt tagaaatgga
    16381 gatcttactg aaggcacagc ctatacaaac gctgttggat ttatgcctaa cctatcagct
    16441 tatccaaaat ctcacggtaa aactgccaaa agtaacattg tcagtcaagt ttacttaaac
    16501 ggagacaaaa ctaaacctgt aacactaacc attacactaa acggtacaca ggaaacagga
    16561 gacacaactc caagtgcata ctctatgtca ttttcatggg actggtctgg ccacaactac
    16621 attaatgaaa tatttgccac atcctcttac actttttcat acattgccca agaataaaga
    16681 atcgtttgtg ttatgtttca acgtgtttat ttttcaattg cagaaaattt caagtcattt
    16741 ttcattcagt agtatagccc caccaccaca tagcttatac agatcaccgt accttaatca
    16801 aactcacaga accctagtat tcaacctgcc acctccctcc caacacacag agtacacagt
    16861 cctttctccc cggctggcct taaaaagcat catatcatgg gtaacagaca tattcttagg
    16921 tgttatattc cacacggttt cctgtcgagc caaacgctca tcagtgatat taataaactc
    16981 cccgggcagc tcacttaagt tcatgtcgct gtccagctgc tgagccacag gctgctgtcc
    17041 aacttgcggt tgcttaacgg gcggcgaagg agaagtccac gcctacatgg gggtagagtc
    17101 ataatcgtgc atcaggatag ggcggtggtg ctgcagcagc gcgcgaataa actgctgccg
    17161 ccgccgctcc gtcctgcagg aatacaacat ggcagtggtc tcctcagcga tgattcgcac
    17221 cgcccgcagc ataaggcgcc ttgtcctccg ggcacagcag cgcaccctga tctcacttaa
    17281 atcagcacag taactgcagc acagcaccac aatattgttc aaaatcccac agtgcaaggc
    17341 gctgtatcca aagctcatgg cggggaccac agaacccacg tggccatcat accacaagcg
    17401 caggtagatt aagtggcgac ccctcataaa cacgctggac ataaacatta cctcttttgg
    17461 catgttgtaa ttcaccacct cccggtacca tataaacctc tgattaaaca tggcgccatc
    17521 caccaccatc ctaaaccagc tggccaaaac ctgcccgccg gctatacact gcagggaacc
    17581 gggactggaa caatgacagt ggagagccca ggactcgtaa ccatggatca tcatgctcgt
    17641 catgatatca atgttggcac aacacaggca cacgtgcata cacttcctca ggattacaag
    17701 ctcctcccgc gttagaacca tatcccaggg aacaacccat tcctgaatca gcgtaaatcc
    17761 cacactgcag ggaagacctc gcacgtaact cacgttgtgc attgtcaaag tgttacattc
    17821 gggcagcagc ggatgatcct ccagtatggt agcgcgggtt tctgtctcaa aaggaggtag
    17881 acgatcccta ctgtacggag tgcgccgaga caaccgagat cgtgttggtc gtagtgtcat
    17941 gccaaatgga acgccggacg tagtcatatt tcctgaagca aaaccaggtg cgggcgtgac
    18001 aaacagatct gcgtctccgg tctcgccgct tagatcgctc tgtgtagtag ttgtagtata
    18061 tccactctct caaagcatcc aggcgccccc tggcttcggg ttctatgtaa actccttcat
    18121 gcgccgctgc cctgataaca tccaccaccg cagaataagc cacacccagc caacctacac
    18181 attcgttctg cgagtcacac acgggaggag cgggaagagc tggaagaacc atgttttttt
    18241 ttttattcca aaagattatc caaaacctca aaatgaagat ctattaagtg aacgcgctcc
    18301 cctccggtgg cgtggtcaaa ctctacagcc aaagaacaga taatggcatt tgtaagatgt
    18361 tgcacaatgg cttccaaaag gcaaacggcc ctcacgtcca agtggacgta aaggctaaac
    18421 ccttcagggt gaatctcctc tataaacatt ccagcacctt caaccatgcc caaataattc
    18481 tcatctcgcc accttctcaa tatatctcta agcaaatccc gaatattaag tccggccatt
    18541 gtaaaaatct gctccagagc gccctccacc ttcagcctca agcagcgaat catgattgca
    18601 aaaattcagg ttcctcacag acctgtataa gattcaaaag cggaacatta acaaaaatac
    18661 cgcgatcccg taggtccctt cgcagggcca gctgaacata atcgtgcagg tctgcacgga
    18721 ccagcgcggc cacttccccg ccaggaacca tgacaaaaga acccacactg attatgacac
    18781 gcatactcgg agctatgcta accagcgtag ccccgatgta agcttgttgc atgggcggcg
    18841 atataaaatg caaggtgctg ctcaaaaaat caggcaaagc ctcgcgcaaa aaagaaagca
    18901 catcgtagtc atgctcatgc agataaaggc aggtaagctc cggaaccacc acagaaaaag
    18961 acaccatttt tctctcaaac atgtctgcgg gtttctgcat aaacacaaaa taaaataaca
    19021 aaaaaacatt taaacattag aagcctgtct tacaacagga aaaacaaccc ttataagcat
    19081 aagacggact acggccatgc cggcgtgacc gtaaaaaaac tggtcaccgt gattaaaaag
    19141 caccaccgac agctcctcgg tcatgtccgg agtcataatg taagactcgg taaacacatc
    19201 aggttgattc acatcggtca gtgctaaaaa gcgaccgaaa tagcccgggg gaatacatac
    19261 ccgcaggcgt agagacaaca ttacagcccc cataggaggt ataacaaaat taataggaga
    19321 gaaaaacaca taaacacctg aaaaaccctc ctgcctaggc aaaatagcac cctcccgctc
    19381 cagaacaaca tacagcgctt ccacagcggc agccataaca gtcagcctta ccagtaaaaa
    19441 agaaaaccta ttaaaaaaac accactcgac acggcaccag ctcaatcagt cacagtgtaa
    19501 aaaagggcca agtgcagagc gagtatatat aggactaaaa aatgacgtaa cggttaaagt
    19561 ccacaaaaaa cacccagaaa accgcacgcg aacctacgcc cagaaacgaa agccaaaaaa
    19621 cccacaactt cctcaaatcg tcacttccgt tttcccacgt tacgtcactt cccattttaa
    19681 gaaaactaca attcccaaca catacaagtt actccgccct aaaacctacg tcacccgccc
    19741 cgttcccacg ccccgcgcca cgtcacaaac tccaccccct cattatcata ttggcttcaa
    19801 tccaaaataa ggtatattat tgatgatgtt aattaagaat tcggatctgc gacgcgaggc
    19861 tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg
    19921 caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca aggccagcaa
    19981 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
    20041 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
    20101 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
    20161 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
    20221 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
    20281 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
    20341 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
    20401 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
    20461 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
    20521 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
    20581 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
    20641 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
    20701 ttcacctaga tccttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
    20761 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
    20821 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
    20881 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
    20941 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
    21001 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
    21061 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
    21121 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
    21181 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
    21241 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
    21301 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
    21361 cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca
    21421 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    21481 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    21541 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    21601 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    21661 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    21721 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
    21781 taacctataa aaataggcgt atcacgaggc cctttcgtct tcaagaatt
//

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