TetO-FUW-OSKM

价格:1200元

联系方式:I47-825O-882O

相关技术服务:质粒构建    基因合成    质粒大提

买家导航

载体基本信息

载体名称: TetO-FUW-OSKM 
出品公司: 爱迪基因
目录编号: 20321
存储实验室: Rudolf Jaenisch

载体骨架基本信息
载体名称: FUW
载体出品公司: homemade
载体抗性: 氨苄霉素
空载体大小: 8376 bp
载体类型: 哺乳细胞表达
筛选标记: 博莱霉素
高拷贝/低拷贝: 高拷贝
生长条件: 37 ℃

插入基因信息
插入基因名称: Oct-4
物种来源: M. musculus (鼠)
插入大小: 5000 bp
克隆方法: 限制性内切酶
5' 克隆位点: EcoRI(未破坏)
3' 克隆位点: EcoRI(未破坏)
5' 测序引物 ACTTTGCAGCCTGAGGGCCA
3' 测序引物  

文献引用方法
材料和方法部分: TetO-FUW-OSKM was a gift from Rudolf Jaenisch (Addgene plasmid # 20321)
参考文献部分: Reprogramming of murine and human somatic cells using a single polycistronic vector. Carey BW, Markoulaki S, Hanna J, Saha K, Gao Q, Mitalipova M, Jaenisch R. Proc Natl Acad Sci U S A. 2009 Jan 6. 106(1):157-62. 10.1073/pnas.0811426106 PubMed 19109433

载体图谱质粒图谱和多克隆位点信息

TetO-FUW-OSKM载体图谱

载体简介

载体序列

LOCUS       TetO-FUW-OSKM Sequencing Result	13462 bp 	DNA	SYN
DEFINITION  TetO-FUW-OSKM Sequencing Result
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..13462
                     /organism="TetO-FUW-OSKM Sequencing Result"
                     /mol_type="other DNA"
     promoter        257..285
                     /label="AmpR_promoter"
     gene            327..1187
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             327..1187
                     /label="ORF frame 3"
     rep_origin      1342..1961
                     /label="pBR322_origin"
     promoter        2270..2299
                     /label="lac_promoter"
     misc_feature    2313..2335
                     /label="M13_pUC_rev_primer"
     promoter        2334..2352
                     /label="M13_reverse_primer"
     misc_feature    complement(2408..2427)
                     /label="EBV_rev_primer"
     misc_feature    complement(2648..3022)
                     /label="sh_ble"
     gene            complement(2651..3022)
                     /label="bleo"
                     /gene="bleo"
     promoter        complement(3023..3090)
                     /label="EM7_promoter"
     misc_feature    complement(3204..3223)
                     /label="SV40pro_F_primer"
     rep_origin      complement(3208..3285)
                     /label="SV40_origin"
     misc_feature    3255..3470
                     /label="SV40_enhancer"
     misc_feature    3450..3470
                     /label="pBABE_3_primer"
     rep_origin      complement(3584..3890)
                     /label="f1_origin"
     terminator      complement(3953..4180)
                     /label="bGH_PA_terminator"
     misc_feature    4166..4183
                     /label="BGH_rev_primer"
     misc_feature    complement(4209..4389)
                     /label="HIV-1_5_LTR"
     misc_feature    complement(4209..4389)
                     /label="truncHIV-1_3_LTR"
     CDS             complement(4363..5412)
                     /label="ORF frame 2"
     misc_feature    complement(4705..4726)
                     /label="U3PPT"
     misc_feature    complement(4711..4726)
                     /label="cPPT"
     CDS             complement(4887..5399)
                     /label="ORF frame 3"
     misc_feature    4894..4910
                     /label="pBluescriptKS_primer"
     misc_feature    complement(4920..5495)
                     /label="WPRE"
     CDS             complement(5535..10598)
                     /label="ORF frame 3"
     CDS             9154..9621
                     /label="ORF frame 1"
     CDS             10391..11062
                     /label="ORF frame 2"
     promoter        complement(10618..10737)
                     /label="CMV2_promoter"
     misc_feature    complement(10680..10704)
                     /label="LNCX_primer"
     misc_feature    complement(10687..10706)
                     /label="pCEP_fwd_primer"
     misc_feature    complement(10739..11056)
                     /label="TRE"
     misc_feature    complement(11197..11212)
                     /label="cPPT"
     CDS             complement(11211..12098)
                     /label="ORF frame 3"
     misc_feature    complement(11743..11976)
                     /label="RRE"
     misc_feature    complement(12486..12530)
                     /label="HIV-1_psi_pack"
     misc_feature    complement(12641..12821)
                     /label="truncHIV-1_3_LTR"
     misc_feature    complement(12641..12821)
                     /label="HIV-1_5_LTR"
     promoter        complement(12849..13417)
                     /label="CMV_immearly_promoter"
     misc_feature    complement(12864..12884)
                     /label="CMV_fwd_primer"
     misc_feature    complement(13050..13337)
                     /label="CAG_enhancer"
ORIGIN
    1 CTAACCCTAA GCAGATTCTT CATGCAATTG TCGGTCAAGC CTTGCCTTGT TGTAGCTTAA
   61 ATTTTGCTCG CGCACTACTC AGCGACCTCC AACACACAAG CAGGGAGCAG ATACTGGCTT
  121 AACTATGCGG CATCAGAGCA GATTGTACTG AGAGTGCACC ATAGGGGATC GGGAGATCTC
  181 CCGATCCGTC GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT
  241 TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC
  301 TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC
  361 CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA
  421 AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG
  481 GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG
  541 TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC
  601 GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA
  661 CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG
  721 CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA
  781 ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC
  841 CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT
  901 TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG
  961 ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA
 1021 AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA
 1081 AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA
 1141 ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG
 1201 TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG
 1261 TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT
 1321 GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG
 1381 TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC
 1441 AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA
 1501 CTGTTCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA
 1561 CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC
 1621 TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG
 1681 GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC
 1741 AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG
 1801 TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT
 1861 ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT
 1921 CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG
 1981 CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA
 2041 ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA
 2101 GCGAGTCAGT GAGCGAGGAA GCGGAAGagc gcccaatacg caaaccgcct ctccccgcgc
 2161 gttggccgat tcattaatgC AGCTGGCACG ACAGGTTTCC CGACTGGAAA GCGGGCAGTG
 2221 AGCGCAACGC AATTAATGTG AGTTAGCTCA CTCATTAGGC ACCCCAGGCT TTACACTTTA
 2281 TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGAGCGGATA ACAATTTCAC ACAGGAAACA
 2341 GCTATGACCA TGATTACGCC AAGCTCTAGC TAGAGGTCGA CGGTATACAG ACATGATAAG
 2401 ATACATTGAT GAGTTTGGAC AAACCACAAC TAGAATGCAG TGAAAAAAAT GCTTTATTTG
 2461 TGAAATTTGT GATGCTATTG CTTTATTTGT AACCATTATA AGCTGCAATA AACAAGTTGG
 2521 GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC
 2581 GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTA
 2641 GCACGTGTCA GTCCTGCTCC TCGGCCACGA AGTGCACGCA GTTGCCGGCC GGGTCGCGCA
 2701 GGGCGAACTC CCGCCCCCAC GGCTGCTCGC CGATCTCGGT CATGGCCGGC CCGGAGGCGT
 2761 CCCGGAAGTT CGTGGACACG ACCTCCGACC ACTCGGCGTA CAGCTCGTCC AGGCCGCGCA
 2821 CCCACACCCA GGCCAGGGTG TTGTCCGGCA CCACCTGGTC CTGGACCGCG CTGATGAACA
 2881 GGGTCACGTC GTCCCGGACC ACACCGGCGA AGTCGTCCTC CACGAAGTCC CGGGAGAACC
 2941 CGAGCCGGTC GGTCCAGAAC TCGACCGCTC CGGCGACGTC GCGCGCGGTG AGCACCGGAA
 3001 CGGCACTGGT CAACTTGGCC ATGGTTTAGT TCCTCACCTT GTCGTATTAT ACTATGCCGA
 3061 TATACTATGC CGATGATTAA TTGTCAACAC GTGCTGATCA GATCCGAAAA TGGATATACA
 3121 AGCTCCCGGG AGCTTTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG
 3181 GAATAGCTCA GAGGCAGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC
 3241 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG
 3301 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA
 3361 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT
 3421 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGAATTAA
 3481 TTCGCGTTAA ATTTTTGTTA AATCAGCTCA TTTTTTAACC AATAGGCCGA AATCGGCAAA
 3541 ATCCCTTATA AATCAAAAGA ATAGACCGAG ATAGGGTTGA GTGTTGTTCC AGTTTGGAAC
 3601 AAGAGTCCAC TATTAAAGAA CGTGGACTCC AACGTCAAAG GGCGAAAAAC CGTCTATCAG
 3661 GGCGATGGCC CACTACGTGA ACCATCACCC TAATCAAGTT TTTTGGGGTC GAGGTGCCGT
 3721 AAAGCACTAA ATCGGAACCC TAAAGGGAGC CCCCGATTTA GAGCTTGACG GGGAAAGCCG
 3781 GCGAACGTGG CGAGAAAGGA AGGGAAGAAA GCGAAAGGAG CGGGCGCTAG GGCGCTGGCA
 3841 AGTGTAGCGG TCACGCTGCG CGTAACCACC ACACCCGCCG CGCTTAATGC GCCGCTACAG
 3901 GGCGCGTGGG GATACCCCCT AGAGCCCCAG CTGGTTCTTT CCGCCTCAGA AGCCATAGAG
 3961 CCCACCGCAT CCCCAGCATG CCTGCTATTG TCTTCCCAAT CCTCCCCCTT GCTGTCCTGC
 4021 CCCACCCCAC CCCCCAGAAT AGAATGACAC CTACTCAGAC AATGCGATGC AATTTCCTCA
 4081 TTTTATTAGG AAAGGACAGT GGGAGTGGCA CCTTCCAGGG TCAAGGAAGG CACGGGGGAG
 4141 GGGCAAACAA CAGATGGCTG GCAACTAGAA GGCACAGTCG AGGCTGATCA GCGGGTTTAA
 4201 ACGGGCCCTG CTAGAGATTT TCCACACTGA CTAAAAGGGT CTGAGGGATC TCTAGTTACC
 4261 AGAGTCACAC AACAGACGGG CACACACTAC TTGAAGCACT CAAGGCAAGC TTTATTGAGG
 4321 CTTAAGCAGT GGGTTCCCTA GTTAGCCAGA GAGCTCCCAG GCTCAGATCT GGTCTAACCA
 4381 GAGAGACCCA GTACAGTCCG GATGCAGCTC TCGGGCCATG TGATGAAATG CTAGGCGGCT
 4441 GTCAAACCTC CACTCTAATA CTTCTCTCTC CGGGTCATCC ATCCCATGCA GGCTCACAGG
 4501 GTGTAACAAG CGGGTGTTCT CTCCTTCATT GGCTTCTTCT ACCTTCTCTT GCTCAACTGG
 4561 TACTAGCTTG TAGCACCATC CAAAGGTCAG TGGATATCTG ATCCCTGGCC CTGGTGTGTA
 4621 GTTCTGCCAA TCAGGGAAGT AGCCTTGTGT GTGGTAGATC CACAGATCAA GGATATCTTG
 4681 TCTTCGTTGG GAGTGAATTA GCCCTTCCAG TCCCCCCTTT TCTTTTAAAA AGTGGCTAAG
 4741 ATCTACAGCT GCCTTGTAAG TCATTGGTCT TAAAGGTACC TGAGGTGTGA CTGGAAAACC
 4801 CACCTCCTCC TCCTCTTGTG CTTCTAGCCA GGCACAATCA GCATTGGTAG CTGCTGTATT
 4861 GCTACTTGTG ATTGCTCCAT GTTTTTCTAG GTCTCGAGGT CGACGGTATC GATGCGGGGA
 4921 GGCGGCCCAA AGGGAGATCC GACTCGTCTG AGGGCGAAGG CGAAGACGCG GAAGAGGCCG
 4981 CAGAGCCGGC AGCAGGCCGC GGGAAGGAAG GTCCGCTGGA TTGAGGGCCG AAGGGACGTA
 5041 GCAGAAGGAC GTCCCGCGCA GAATCCAGGT GGCAACACAG GCGAGCAGCC AAGGAAAGGA
 5101 CGATGATTTC CCCGACAACA CCACGGAATT GTCAGTGCCC AACAGCCGAG CCCCTGTCCA
 5161 GCAGCGGGCA AGGCAGGCGG CGATGAGTTC CGCCGTGGCA ATAGGGAGGG GGAAAGCGAA
 5221 AGTCCCGGAA AGGAGCTGAC AGGTGGTGGC AATGCCCCAA CCAGTGGGGG TTGCGTCAGC
 5281 AAACACAGTG CACACCACGC CACGTTGCCT GACAACGGGC CACAACTCCT CATAAAGAGA
 5341 CAGCAACCAG GATTTATACA AGGAGGAGAA AATGAAAGCC ATACGGGAAG CAATAGCATG
 5401 ATACAAAGGC ATTAAAGCAG CGTATCCACA TAGCGTAAAA GGAGCAACAT AGTTAAGAAT
 5461 ACCAGTCAAT CTTTCACAAA TTTTGTAATC CAGAGGTTGA TTATCGATAA GCTTGATATC
 5521 GAATTCGGCG CGCCTTATGC ACCAGAGTTT CGAAGCTGTT CGAGTTTGTG TTTCAACTGT
 5581 TCTCGTCGTT TCCTCAATAA GTCCTTTTCA GAGGTGAGCT TGTGCTCGTC TGCTTGAATG
 5641 GACAGGATGT AGGCGGTGGC TTTTTTGAGG ATCACTACCT TGGGGGCCTT TTCGTTGTTT
 5701 TCCAATTCAG GGATCTGGTC ACGCAGGGCA AAAAAGCTGC GCTTCAGCTC GTTCCTCCTC
 5761 TGACGTTCCA AGACGTTGTG TGTCCGCCTC TTGTCGTTTT CCTCCGTGTC TGAGGACCTG
 5821 GGGCTGGAGC ACTTGCGGTT GTTGCTGATC TGCTTCAGGA CCCTGCCACT GTCCAACTTG
 5881 GCCCTCTTGG CAGCTGGATA GTCCTTCCTT GTGGAGGGGG GTGCGGCGTA GTTGTGCTGG
 5941 TGAGTGGAGA CGTGGCACCT CTTGAGGACC AGTGGGCTGT GCGGAGGTTT GCTGTGGCCT
 6001 CGGGATGGAG ATGAGCCCGA CTCCGACCTC TTGGCAGGGG TTTGCCTCTT CTCCACAGAC
 6061 ACCACATCAA TTTCTTCCTC ATCTTCTTGC TCTTCTTCAG AGTCGCTGCT GGTGGTGGGC
 6121 GGTGTCTCCT CATGCAGCAC TAGGGGCTCA GGGCTGGCCC GTGGGGAGGA CTCGGAGGAC
 6181 AGCAGCGAGT CCGAGGAAGG AGAGAAGGCC GTGGAATCGG ACGAGGTACA GGATTTGGGC
 6241 GAGCTGCTGT CGTTGAGCGG GTAGGGAAAG ACCACTGAGG GGTCAATGCA CTCGGACGCG
 6301 GCGGCGGTGA GGTCCTGCAG GTACAGGCTG GAGGTGGAGC AGACGCTGTG CCCGCGGGCG
 6361 GGGCTCAGGC TGGTGCTGTC TTTGCGCGCA GCCTGGTAGG AGGCCAGCTT CTCCGAGACC
 6421 AGCTTGGCAG CGGCTGAGAA ACCGCTCCAC ATACAGTCCT GGATGATGAT GTTCTTGATG
 6481 AAGGTCTCGT CGTCAGGATC GCAGATGAAG CTCTGGTTCA CCATGTCTCC TCCAAGTAAC
 6541 TCGGTCATCA TCTCCAGCTG ATCGGCGGTG GAGAAGTTGC CACCGCCGCC GTCATCGTCT
 6601 TCCCTTGGGG AGAAGGACGT AGCGACCGCA ACATAGGATG GAGAGCAGAG CCCGGAGCGG
 6661 CGGCTCGGGG ACAGGGGCGG GGTGGGAAGC AGCTCGAATT TCTTCCAGAT ATCCTCACTG
 6721 GGCGCGGGCG GCTGCAGCTC GCTCTGCTGT TGCTGGTGAT AGAAATTCTC TTCCTCGTCG
 6781 CAGATGAAAT AGGGCTGTAC GGAGTCGTAG TCGAGGTCAT AGTTCCTGTT GGTGAAGTTC
 6841 ACGTTGAGGG GCATCGTCGT GGCTGTCTGC GGGGTTTCCA ACGCCCAAAG GAAATCCAGT
 6901 GGCGCGCCGG GACCTGGGTT GCTCTCAACA TCTCCAGCCA ATTTCAAGAG AGCATAATTA
 6961 GTACACTGGC CCGAGCCCTC GAGAAAGTGC CTCTTCATGT GTAAGGCAAG GTGGTCCGAC
 7021 CTGGAAAAGG CCCTGTCGCA CTTCTGGCAC TGAAAGGGCC GGTGCCCTGT GTGTTTGCGG
 7081 TAGTGCCTGG TCAGTTCATC GGAGCGGGCG AATTTCCACC CACAGCCGTC CCAGTCACAG
 7141 TGGTAAGGTT TCTCGCCTGT GTGAGTTCGC AGGTGTGCCT TGAGATGAGA ACTCTTGGTA
 7201 TAGGTTTTGC CACAGCCTGC ATAGTCACAA GTGTGGGTGG CTGTTCTTTT CCGGGGCCAC
 7261 GACCTTCTTC CCCTCTTTGG CTTGGGCTCC TCTGGCAGGC AGGAACCCGG TGGCATGAGC
 7321 TCTTGATAAT GGAGAGAGGG GACTTGTGAC TGCATCTGGT CTGGCAGGAA AGGAGGGTAG
 7381 TTGGGCCCCG GATGGGGATG GAATCCTGGG GGAAGAGGCA GGCCAGGGTG ACAGTCCCTG
 7441 CTGTTCAGCA GTTCCTCGGG ACTCAGTGTA GGGGTAGTCC TGGTGGGGAG CTGCCGCCCC
 7501 AGGGGGAAGT CGTGTGTGTT GGGCCGGTGG CCGTTGCTGA GCTGGGGTCC AGCGCTCAAA
 7561 TGGGCCTCTA GGGACCGGCT GACCGTGCAG GACGGGACCG CCTCTTGCTT AATCTTGGGG
 7621 CACATGCGCG GCGGGCCACC GCTGTAGGGC GCCACTACCA CGGGGTGGCT GCCGTCTGGG
 7681 CTTCCTTTGC TAACACTGAT GACCGAAGGG CTGCTGTACT CGCTGCCAGG GGTGGTCAGA
 7741 GACGCCTTCA GCACAAACTT GCCCATCAGC CCGCCACCTG GCGGCTGAGG CTGCTGTGGC
 7801 GGAATGTATA CTGGGTCCAA CTCCGGCCGC AGGAGCTCAG CCACGAAGCC GCCCGAGGGG
 7861 CTCACGTCAT TGATGTCCGC CAGGTTGAAG GGGGCCGTGG GAGGTGGCGC AGATTCTCGG
 7921 CTGTAGAGGA GCCCTCCACC TGTGTTGCTG GCAGCCACGC CCGGGTCACC CCCGGCCCGG
 7981 ATCGGATAGC TGAAGCTGCA GGTGGAGGGC GCGCTGGCAG GGCCGCTGCT CGCTGGGGAA
 8041 GACGAGGATG AAGCTGACGC CGAGGTGGTC ACGGTGGCGG CCACCGATTC CTGGTGGGTT
 8101 AGCGAGTTGG AAAGGATAAA GTCTAGGTCC AGGAGGTCGT TGAACTCCTC GGTCTCCCTC
 8161 CGGGCTAGGA GGGCCGGGTT GTTACTGCTG CAAGCTGCAC CAGCTCCGCC ACTCTCCAGG
 8221 TCTGTGGCCA CCGTCGCCGC CAGGTCGTAG GGGCGGCCGG GAAGTGGGGG AAGTCGCTTC
 8281 ATGTGAGAGA GTTCCTCACG CCAACGGTTA GTCGGGGCAC CTGCTGGACG CAGTGTCTTC
 8341 TCCCTTCCCG CCGGGCCGGA CGCGAACGTG GAGAAGGACG GGAGCAGAGC GTCGCTGACA
 8401 GCCATGTCAG ACTCGCCAGG TGGCTGCCTC ATCTCGAGTG GGCCGGGATT TTCCTCCACG
 8461 TCCCCGCATG TTAGAAGACT TCCCCTGCCC TCGCCGGAGC CGCATGCCAT GTGCGACAGG
 8521 GGCAGTGTGC CGTTAATGGC CGTGCCGGGC ACCGGGCCGC TCTGGTAGTG CTGGGCCATG
 8581 TGCAGTCTAC TGGGCGCAGC GGGCTCCGGC ACCTCGGCGC CGGGGAGGTA CATGCTGATC
 8641 ATGTCCCGGA GGTCCCCGGC CTGGCAGGGC GCCCTGGAGT GGGAGGAAGA GGTAACCACG
 8701 GGGGGGCTGG AGCTGGCCTC GGACTTGACC ACAGAGCCCA TGGAGCCCAG CGCCATACCG
 8761 GGGGTGCCCT GCTGCGAGTA GGACATGCTG TAGGTGGGCG AGCCGTTCAT GTAGGTCTGC
 8821 GAGCTGGTCA TGGAGTTGTA CTGCAGGGCG CTGACGTCGT AGCGGTGCAT CGGTTGCATC
 8881 TGTGCCGCGC CGTGAGCGTT GAGGCCCGGG TGCTGCGGGT AGCCCAGCTG CTCCTGCATC
 8941 ATGCTGTAGC TGCCGTTGCT CCAGCCGTTC ATGTGCGCGT AGCTGTCCAT GCGCTGGTTC
 9001 ACGCCCGCAC CCAGGCCGGC GCCCACCCCA ACCCCGCTCG CCATGCTGTT CCCGCCGGGG
 9061 GCCAGCAAGC CTCCGGGAAG CGTGTACTTA TCCTTCTTCA TGAGCGTCTT GGTTTTCCGC
 9121 CGCGGCCGGT ATTTATAATC CGGGTGCTCC TTCATGTGCA GAGCGCGCAG CCGCTTGGCC
 9181 TCGTCGATGA ACGGCCGCTT CTCGGTCTCG GACAAAAGTT TCCACTCCGC GCCCAGGCGC
 9241 TTGCTGATCT CCGAGTTGTG CATCTTGGGG TTCTCCTGGG CCATCTTACG CCGCTGCCCC
 9301 CGGGACCATA CCATGAAGGC GTTCATGGGC CTCTTGACGC GGTCCGGGCT GTTCTTCTGG
 9361 TTGCCGCCGG TCGCCGCCGC CGTGGCGTTG CCTCCTCCGC CGCCGCCCCC CGAAGCTTGC
 9421 TGCGGGCCCG GCGGCTTCAG CTCCGTCTCC ATCATGTTAT ACATGCATGC AGGCCCGGGG
 9481 TTTTCTTCAA CATCTCCTGC TTGCTTTAAC AGAGAGAAGT TCGTGGCGCC GCTGCCGCTA
 9541 GCGTTTGAAT GCATGGGAGA GCCCAGAGCA GTGACGGGAA CAGAGGGAAA GGCCTCGCCC
 9601 TCAGGAAAAG GGACTGAGTA GAGTGTGGTG AAGTGGGGGC TTCCATAGCC TGGGGTGCCA
 9661 AAGTGGGGAC CTGGGGGCAG AGGAAAGGAT ACAGCCCCCC CTGGGAAAGG TGTCCCTGTA
 9721 GCCTCATACT CTTCTCGTTG GGAATACTCA ATACTTGATC TTTTGCCCTT CTGGCGCCGG
 9781 TTACAGAACC ATACTCGAAC CACATCCTTC TCTAGCCCAA GCTGATTGGC GATGTGAGTG
 9841 ATCTGCTGTA GGGAGGGCTT CGGGCACTTC AGAAACATGG TCTCCAGACT CCACCTCACA
 9901 CGGTTCTCAA TGCTAGTTCG CTTTCTCTTC CGGGCCTGCA CCAGGGTCTC CGATTTGCAT
 9961 ATCTCCTGAA GGTTCTCATT GTTGTCGGCT TCCTCCACCC ACTTCTCCAG CAGGGGCCGC
10021 AGCTTACACA TGTTCTTAAG GCTGAGCTGC AAGGCCTCGA AGCGACAGAT GGTGGTCTGG
10081 CTGAACACCT TTCCAAAGAG AACGCCCAGG GTGAGCCCCA CGTCGGCCTG GGTGTACCCC
10141 AAGGTGATCC TCTTCTGCTT CAGCAGCTTG GCAAACTGTT CTAGCTCCTT CTGCAGGGCT
10201 TTCATGTCCT GGGACTCCTC GGGAGTTGGT TCCACCTTCT CCAACTTCAC GGCATTGGGG
10261 CGGTCGGCAC AGGGCTCAGA GGAGGTTCCC TCTGAGTTGC TTTCCACTCG TGCTCCTGCC
10321 TGGCCCTCAG GCTGCAAAGT CTCCACGCCA ACTTGGGGGA CTAGGCCCAG TCCAACCTGA
10381 GGTCCACAGT ATGCCATCCC TCCGCAGAAC TCGTATGCGG GCGGACATGG GGAGATCCCC
10441 AATACCTCTG AGCCTGGTCC GATTCCAGGC CCACCTGGAG GCCCTTGGAA GCTTAGCCAG
10501 GTTCGAGGAT CCACCCAGCC CGGCTCCAGC CCTGCTGACC CATCACCCCC ACCTGGTGGG
10561 GGTGAGAAGG CGAAGTCTGA AGCCAGGTGT CCAGCCATGG CGAATTCGGG GCCGCGGAGG
10621 CTGGATCGGT CCCGGTGTCT TCTATGGAGG TCAAAACAGC GTGGATGGCG TCTCCAGGCG
10681 ATCTGACGGT TCACTAAACG AGCTCTGCTT ATATAGGCCT CCCACCGTAC ACGCCTACCT
10741 CGACCCGGGT ACCGAGCTCG ACTTTCACTT TTCTCTATCA CTGATAGGGA GTGGTAAACT
10801 CGACTTTCAC TTTTCTCTAT CACTGATAGG GAGTGGTAAA CTCGACTTTC ACTTTTCTCT
10861 ATCACTGATA GGGAGTGGTA AACTCGACTT TCACTTTTCT CTATCACTGA TAGGGAGTGG
10921 TAAACTCGAC TTTCACTTTT CTCTATCACT GATAGGGAGT GGTAAACTCG ACTTTCACTT
10981 TTCTCTATCA CTGATAGGGA GTGGTAAACT CGACTTTCAC TTTTCTCTAT CACTGATAGG
11041 GAGTGGTAAA CTCGAGATCT AACCAAACTG GATCTCTGCT GTCCCTGTAA TAAACCCGAA
11101 AATTTTGAAT TTTTGTAATT TGTTTTTGTA ATTCTTTAGT TTGTATGTCT GTTGCTATTA
11161 TGTCTACTAT TCTTTCCCCT GCACTGTACC CCCCAATCCC CCCTTTTCTT TTAAAATTGT
11221 GGATGAATAC TGCCATTTGT CTGCAGAATT GGCGCACGCA GTGCCGATCC GTTCACTAAT
11281 CGAATGGATC TGTCTCTGTC TCTCTCTCCA CCTTCTTCTT CTATTCCTTC GGGCCTGTCG
11341 GGTCCCCTCG GGGTTGGGAG GTGGGTCTGA AACGATAATG GTGAATATCC CTGCCTAACT
11401 CTATTCACTA TAGAAAGTAC AGCAAAAACT ATTCTTAAAC CTACCAAGCC TCCTACTATC
11461 ATTATGAATA ATTTTATATA CCACAGCCAA TTTGTTATGT TAAACCAATT CCACAAACTT
11521 GCCCATTTAT CTAATTCCAA TAATTCTTGT TCATTCTTTT CTTGCTGGTT TTGCGATTCT
11581 TCAATTAAGG AGTGTATTAA GCTTGTGTAA TTGTTAATTT CTCTGTCCCA CTCCATCCAG
11641 GTCGTGTGAT TCCAAATCTG TTCCAGAGAT TTATTACTCC AACTAGCATT CCAAGGCACA
11701 GCAGTGGTGC AAATGAGTTT TCCAGAGCAA CCCCAAATCC CCAGGAGCTG TTGATCCTTT
11761 AGGTATCTTT CCACAGCCAG GATTCTTGCC TGGAGCTGCT TGATGCCCCA GACTGTGAGT
11821 TGCAACAGAT GCTGTTGCGC CTCAATAGCC CTCAGCAAAT TGTTCTGCTG CTGCACTATA
11881 CCAGACAATA ATTGTCTGGC CTGTACCGTC AGCGTCATTG ACGCTGCGCC CATAGTGCTT
11941 CCTGCTGCTC CCAAGAACCC AAGGAACAAA GCTCCTATTC CCACTGCTCT TTTTTCTCTC
12001 TGCACCACTC TTCTCTTTGC CTTGGTGGGT GCTACTCCTA ATGGTTCAAT TTTTACTACT
12061 TTATATTTAT ATAATTCACT TCTCCAATTG TCCCTCATAT CTCCTCCTCC AGGTCTGAAG
12121 ATCAGCGGCC GCTTGCTGTG CGGTGGTCTT ACTTTTGTTT TGCTCTTCCT CTATCTTGTC
12181 TAAAGCTTCC TTGGTGTCTT TTATCTCTAT CCTTTGATGC ACACAATAGA GGGTTGCTAC
12241 TGTATTATAT AATGATCTAA GTTCTTCTGA TCCTGTCTGA AGGGATGGTT GTAGCTGTCC
12301 CAGTATTTGT CTACAGCCTT CTGATGTTTC TAACAGGCCA GGATTAACTG CGAATCGTTC
12361 TAGCTCCCTG CTTGCCCATA CTATATGTTT TAATTTATAT TTTTTCTTTC CCCCTGGCCT
12421 TAACCGAATT TTTTCCCATC GCGATCTAAT TCTCCCCCGC TTAATACTGA CGCTCTCGCA
12481 CCCATCTCTC TCCTTCTAGC CTCCGCTAGT CAAAATTTTT GGCGTACTCA CCAGTCGCCG
12541 CCCCTCGCCT CTTGCCGTGC GCGCTTCAGC AAGCCGAGTC CTGCGTCGAG AGAGCTCCTC
12601 TGGTTTCCCT TTCGCTTTCA AGTCCCTGTT CGGGCGCCAC TGCTAGAGAT TTTCCACACT
12661 GACTAAAAGG GTCTGAGGGA TCTCTAGTTA CCAGAGTCAC ACAACAGACG GGCACACACT
12721 ACTTGAAGCA CTCAAGGCAA GCTTTATTGA GGCTTAAGCA GTGGGTTCCC TAGTTAGCCA
12781 GAGAGCTCCC AGGCTCAGAT CTGGTCTAAC CAGAGAGACC CAGTACAGGC AAAACGCGCT
12841 GCTTATATAG ACCTCCCACC GTACACGCCT ACCGCCCATT TGCGTCAATG GGGCGGAGTT
12901 GTTACGACAT TTTGGAAAGT CCCGTTGATT TTGGTGCCAA AACAAACTCC CATTGACGTC
12961 AATGGGGTGG AGACTTGGAA ATCCCCGTGA GTCAAACCGC TATCCACGCC CATTGATGTA
13021 CTGCCAAAAC CGCATCACCA TGGTAATAGC GATGACTAAT ACGTAGATGT ACTGCCAAGT
13081 AGGAAAGTCC CATAAGGTCA TGTACTGGGC ATAATGCCAG GCGGGCCATT TACCGTCATT
13141 GACGTCAATA GGGGGCGTAC TTGGCATATG ATACACTTGA TGTACTGCCA AGTGGGCAGT
13201 TTACCGTAAA TACTCCACCC ATTGACGTCA ATGGAAAGTC CCTATTGGCG TTACTATGGG
13261 AACATACGTC ATTATTGACG TCAATGGGCG GGGGTCGTTG GGCGGTCAGC CAGGCGGGCC
13321 ATTTACCGTA AGTTATGTAA CGCGGAACTC CATATATGGG CTATGAACTA ATGACCCCGT
13381 AATTGATTAC TATTAATAAC TAGTCAATAA TCAATGTCAA CGCGTATATC TGGCCCGTAC
13441 ATCGCGAAGC AGCGCAAAAC GC
//

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