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pcDNA3.1+/N-GST(TEV)载体质粒基本信息
pcDNA3.1+/N-GST(TEV)质粒图谱载体图谱和pcDNA3.1+/N-GST(TEV)载体序列质粒序列多克隆位点信息
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pcDNA3.1+/N-GST(TEV)质粒载体简介
pcDNA3.1+/N-GST(TEV)质粒序列载体序列
LOCUS pcDNA3.1+ N-GST(TEV) 6092 bp ds-DNA circular SYN 21-MAY-2015
DEFINITION Mammalian vector with a CMV promoter for expressing proteins with an
N-terminal and TEV protease-cleavable GST tag.
ACCESSION .
VERSION .
KEYWORDS pcDNA3.1+ N-GST(TEV)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6092)
AUTHORS GenScript
TITLE Direct Submission
JOURNAL Exported Tuesday, February 4, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6092
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
enhancer 235..614
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 863..881
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 923..1576
/codon_start=1
/product="glutathione S-transferase from Schistosoma
japonicum"
/note="GST"
/translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
CDS 1586..1606
/codon_start=1
/product="tobacco etch virus (TEV) protease recognition and
cleavage site"
/note="TEV site"
/translation="ENLYFQG"
misc_feature 1607..1663
/note="MCS"
/note="multiple cloning site"
polyA_signal 1689..1913
/note="bGH poly(A) signal"
/note="bovine growth hormone polyadenylation signal"
rep_origin 1959..2387
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2401..2730
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 2581..2716
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 2797..3591
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 3765..3886
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(4337..4925)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5096..5956)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5957..6061)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagcttgcca ccatgtcccc tatactaggt tattggaaaa ttaagggcct
961 tgtgcaaccc actcgacttc ttttggaata tcttgaagaa aaatatgaag agcatttgta
1021 tgagcgcgat gaaggtgata aatggcgaaa caaaaagttt gaattgggtt tggagtttcc
1081 caatcttcct tattatattg atggtgatgt taaattaaca cagtctatgg ccatcatacg
1141 ttatatagct gacaagcaca acatgttggg tggttgtcca aaagagcgtg cagagatttc
1201 aatgcttgaa ggagcggttt tggatattag atacggtgtt tcgagaattg catatagtaa
1261 agactttgaa actctcaaag ttgattttct tagcaagcta cctgaaatgc tgaaaatgtt
1321 cgaagatcgt ttatgtcata aaacatattt aaatggtgat catgtaaccc atcctgactt
1381 catgttgtat gacgctcttg atgttgtttt atacatggac ccaatgtgcc tggatgcgtt
1441 cccaaaatta gtttgtttta aaaaacgtat tgaagctatc ccacaaattg ataagtactt
1501 gaaatccagc aagtatatag catggccttt gcagggctgg caagccacgt ttggtggtgg
1561 cgaccatcct ccaaaatcgg atctggaaaa cctgtatttt cagggcggat ccgaattctg
1621 cagatatcca gcacagtggc ggccgctcga gtctagaggg cccgtttaaa cccgctgatc
1681 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
1741 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
1801 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
1861 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
1921 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
1981 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
2041 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
2101 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
2161 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
2221 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
2281 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
2341 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
2401 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
2461 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
2521 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
2581 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
2641 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
2701 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
2761 ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
2821 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
2881 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
2941 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg
3001 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
3061 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
3121 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
3181 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
3241 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
3301 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat
3361 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
3421 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
3481 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
3541 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
3601 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca
3661 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
3721 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
3781 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
3841 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
3901 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
3961 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
4021 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
4081 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
4141 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
4201 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
4261 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
4321 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
4381 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
4441 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
4501 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
4561 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
4621 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
4681 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
4741 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
4801 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
4861 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
4921 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
4981 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
5041 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
5101 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
5161 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
5221 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
5281 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
5341 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
5401 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
5461 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
5521 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
5581 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
5641 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
5701 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
5761 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
5821 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
5881 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
5941 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
6001 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
6061 gcacatttcc ccgaaaagtg ccacctgacg tc
//
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pcDNA3.1+/N-GST(TEV)
2020-02-04
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