| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-TCOF1-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001660 |
| 产品用途: | 3' AAVLINK plasmid for TCOF1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | TCOF1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv1-pCALM1-Cre-3'-TCOF1-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv1-pCALM1-Cre-3'-TCOF1-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-TCOF1-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-TCOF1-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-TCOF1-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-TCOF1-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-TCOF1-FLAG 6630 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6630)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 1, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6630
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3343
/codon_start=1
/label=TCOF1
/translation="GAVRRSQTVRRRRRRWLR*SLQGRPTRSELPWLLPRSPPGKGLPQ
HLLGRQGLRLPRQGSRMTQGAAARNQTVMGRHRQL*PLPR*LNPL*FLSTLIVVQLAQL
LHPHKPRLQAPRGRPEPRRAQPGAPPPRARMRT*SPLHSA*LLASEPMW*PCPLPTQE*
PPKPAWLGPAAARSPVGYQMARNRRDQPLRCQRRTQLPSH*PRLP*RSSPRKPVRLSLL
LPGPSLQVGLTVLWEHSLQQVPRAPPSRPKGPTSSENLSFLRSSRPPKPLRAQMTVRTA
ATVLQGVRKMVKGPRGPSQPTRW*VPPPPGQRPWWRRPQQSPARMMWWRHPSLSSQVI*
PLD*PQPIPRPQKPLPS*TPAPQFPLLWPPKMTQMASRRQSPNRQQACCPLKQVEKRLL
QAPHLRSPGSPRKGLGTPKPQPWRCKATSPSASWANPGP*MRPRCRPQW*RS*LSCWNR
KERRWWTPPRRAAGRAGRAASGSYRETSQLPGPPGARRRRSWGPGKVGRPLFPQKRPPR
LPRGKQRETKQVVMSRRRKGRGLLAPKGPRTSQKRSFRRGWGRLKVEIKATQRARRRRR
NPTREKKTKKKKKRRRKQKRPQPKILSHRPRRKRRKRRRQQSRL"
misc_feature 1491..1496
/label=SDS
misc_feature 1509..1514
/label=SDS
misc_feature 1512..1517
/label=SDS
misc_feature 1619..1624
/label=SDS
misc_feature 1866..1871
/label=SDS
misc_feature 2040..2045
/label=SDS
misc_feature 2141..2146
/label=SDS
misc_feature 2613..2618
/label=SDS
misc_feature 2773..2778
/label=SDS
CDS 3350..3415
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3419..3850
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3563..3568
/label=SDS
repeat_region 3893..4033
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3893..4022
/label=AAV2 ITR
rep_origin 4108..4563
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4845..4949
/gene="bla"
/label=AmpR promoter
CDS 4950..5810
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5981..6569
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca gggagcagtg aggaggagtc
1501 agacagtgag gaggaggcgg agacgctggc tcaggtgaag ccttcaggga agacccacca
1561 gatcagagct gccttggctc ctgccaagga gtcccccagg aaaggggctg ccccaacacc
1621 tcctgggaag acagggcctt cggctgccca ggcagggaag caggatgact cagggagcag
1681 cagcgaggaa tcagacagtg atggggaggc accggcagct gtgacctctg cccaggtgat
1741 taaaccccct ctgatttttg tcgaccctaa tcgtagtcca gctggcccag ctgctacacc
1801 cgcacaagcc caggctgcaa gcaccccgag gaaggcccga gcctcggaga gcacagccag
1861 gagctcctcc tccgagagcg aggatgagga cgtgatcccc gctacacagt gcttgactcc
1921 tggcatcaga accaatgtgg tgaccatgcc cactgcccac ccaagaatag cccccaaagc
1981 cagcatggct ggggccagca gcagcaagga gtccagtcgg atatcagatg gcaagaaaca
2041 ggagggacca gccactcagg tgtcaaagaa gaacccagct tccctcccac tgacccaggc
2101 tgccctgaag gtcctcgccc agaaagccag tgaggctcag cctcctgttg ccaggaccca
2161 gccttcaagt ggggttgaca gtgctgtggg aacactccct gcaacaagtc cccagagcac
2221 ctccgtccag gccaaaggga ccaacaagct cagaaaacct aagcttcctg aggtccagca
2281 ggccaccaaa gcccctgaga gctcagatga cagtgaggac agcagcgaca gttcttcagg
2341 gagtgaggaa gatggtgaag ggccccaggg ggccaagtca gcccacacgc tggtaggtcc
2401 caccccctcc aggacagaga ccctggtgga ggagaccgca gcagagtcca gcgaggatga
2461 tgtggtggcg ccatcccagt ctctcctctc aggttatatg acccctggac taaccccagc
2521 caattcccag gcctcaaaag ccactcccaa gctagactcc agcccctcag tttcctctac
2581 tctggccgcc aaagatgacc cagatggcaa gcaggaggca aagccccaac aggcagcagg
2641 catgttgtcc cctaaaacag gtggaaaaga ggctgcttca ggcaccacac ctcagaagtc
2701 ccggaagccc aagaaagggg ctgggaaccc ccaagcctca accctggcgc tgcaaagcaa
2761 catcacccag tgcctcctgg gccaaccctg gcccctgaat gaggcccagg tgcaggcctc
2821 agtggtgaag gtcctgactg agctgctgga acaggaaaga aagaaggtgg tggacaccac
2881 caaggagagc agcaggaagg gctgggagag ccgcaagcgg aagctatcgg gagaccagcc
2941 agctgccagg acccccagga gcaagaagaa gaagaagctg ggggccgggg aaggtgggga
3001 ggcctctgtt tccccagaaa agacctccac gacttccaag gggaaagcaa agagagacaa
3061 agcaagtggt gatgtcaagg agaagaaagg gaaggggtct cttggctccc aaggggccaa
3121 ggacgagcca gaagaggagc ttcagaaggg gatggggacg gttgaaggtg gagatcaaag
3181 caacccaaag agcaagaagg agaagaagaa atccgacaag agaaaaaaag acaaagaaaa
3241 aaaagaaaag aagaagaaag caaaaaaggc ctcaaccaaa gattctgagt caccgtccca
3301 gaagaaaaag aagaaaaaga agaagacagc agagcagact gtatgtacag actacaaaga
3361 ccatgacggt gattataaag atcatgacat cgactacaag gatgacgatg acaagtaact
3421 cgagataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
3481 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
3541 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttagttct tgccacggcg
3601 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
3661 aattccgtgg tgtttatttg tgaaatttgt gatgctattg ctttatttgt aaccatctag
3721 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
3781 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg agatgtggga
3841 ggttttttaa agatctggag accggtgtgg cacgtgcgga ccgagcggcc gcaggaaccc
3901 ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga
3961 ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc
4021 agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
4081 caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
4141 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccttagcg cccgctcctt
4201 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
4261 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
4321 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
4381 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaact
4441 ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggtc tattggttaa
4501 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa
4561 ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
4621 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
4681 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
4741 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
4801 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
4861 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
4921 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
4981 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
5041 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
5101 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
5161 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
5221 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
5281 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
5341 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
5401 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
5461 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
5521 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
5581 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
5641 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
5701 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
5761 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
5821 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
5881 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
5941 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
6001 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
6061 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
6121 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
6181 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
6241 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
6301 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
6361 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
6421 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
6481 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
6541 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
6601 tggccttttg ctggcctttt gctcacatgt
//
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