pcambia1305.1

价格:1500元

联系方式:I47-825O-882O

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载体基本信息

出品公司: CAMBIA
载体名称: pCAMBIA1305.1
质粒类型: 植物载体;农杆菌双元表达载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: Lac
载体大小: 11847 bp
5' 测序引物及序列: M13 Reverse:CAGGAAACAGCTATGAC
3' 测序引物及序列: M13/pUC Forward:CCCAGTCACGACGTTGTAAAACG
载体标签: --
载体抗性: 卡那霉素
筛选标记: 潮霉素(Hygromycin)、GUS
克隆菌株: HB101等菌株
宿主细胞(系): 植物细胞、农杆菌
备注: --
产品目录号: --
稳定性: 稳表达
组成型/诱导型: 诱导型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pCAMBIA1305.1载体图谱



pCAMBIA1305.1 多克隆位点

载体简介

载体序列

LOCUS       AF354045               11846 bp    DNA     circular SYN 16-APR-2001
DEFINITION  Binary vector pCAMBIA-1305.1, complete sequence.
ACCESSION   AF354045
VERSION     AF354045.1  GI:13540346
KEYWORDS    .
SOURCE      Binary vector pCAMBIA-1305.1
  ORGANISM  Binary vector pCAMBIA-1305.1
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 11846)
  AUTHORS   Jefferson,R.A., Kilian,A. and Keese,P.K.
  TITLE     Microbial genes for secreted beta-glucuronidases, gene products and
            uses thereof
  JOURNAL   Patent: PCT (PCT/US98/19217)-A 09-SEP-1998;
            CAMBIA; Molecular Technologies; Canberra;
            Australia;
REFERENCE   2  (bases 1 to 11846)
  AUTHORS   Nguyen,T.A. and Jefferson,R.A.
  TITLE     Binary vector pCAMBIA-1305.1
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 11846)
  AUTHORS   Nguyen,T.A. and Jefferson,R.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-FEB-2001) Molecular Technologies, CAMBIA, GPO Box
            3200, Canberra, ACT 2601, Australia
FEATURES             Location/Qualifiers
     source          1..11846
                     /organism="Binary vector pCAMBIA-1305.1"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:156490"
     CDS             join(2..16,207..2054)
                     /note="GUSPlus-His6"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="AAK29426.1"
                     /db_xref="GI:13540350"
                     /translation="MVDLRNRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYE
                     SKLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHK
                     AIVYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHE
                     EGLGKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQ
                     GKAETVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTID
                     VYEEPFGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWI
                     GANSFRTAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEK
                     IRTFEHHQDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQK
                     RPVTIVLFVMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRC
                     PGKPIMITEYGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFAD
                     FATSQGVMRVQGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKNASHHHHHHV"
     intron          17..206
                     /note="catalase intron"
     polyA_signal    2083..2335
                     /note="poly(A) signal from nopaline synthase"
     misc_feature    2354..2378
                     /note="T-DNA right border"
     misc_feature    3439..4439
                     /note="sta region from pVS1 plasmid"
     rep_origin      5032..6032
                     /note="pVS1 rep"
     misc_feature    6442..6702
                     /note="pBR322 bom site"
     rep_origin      6842..7122
                     /note="pBR322 ori"
     CDS             complement(7413..8207)
                     /note="kanamycin resistance gene"
                     /codon_start=1
                     /transl_table=11
                     /product="neomycin phosphotransferase"
                     /protein_id="AAK29424.1"
                     /db_xref="GI:13540348"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    8632..8657
                     /note="T-DNA left border"
     polyA_site      8798..8805
     CDS             complement(8948..9973)
                     /note="hygromycin resistance gene"
                     /codon_start=1
                     /transl_table=11
                     /product="hygromycin phosphotransferase"
                     /protein_id="AAK29425.1"
                     /db_xref="GI:13540349"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKK"
     promoter        complement(10009..10816)
                     /note="CaMV35Sx2; CaMV35S eukaryotic promoter with
                     duplicated enhancer region"
     promoter        10938..11018
                     /note="PlacZ prokaryotic promoter"
     CDS             11019..11252
                     /note="beta-galactosidase alpha fragment"
                     /codon_start=1
                     /transl_table=11
                     /product="lacZ alpha fragment"
                     /protein_id="AAK29423.1"
                     /db_xref="GI:13540347"
                     /translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLN
                     RLAAHPPFASWRNSEEARTDRPSQQLRSLNGEC"
     misc_feature    11033..11089
                     /note="pUC18 polylinker multiple cloning site"
     promoter        11303..11840
                     /note="CaMV35S eukaryotic promoter"
ORIGIN      
        1 catggtagat ctgagggtaa atttctagtt tttctccttc attttcttgg ttaggaccct
       61 tttctctttt tatttttttg agctttgatc tttctttaaa ctgatctatt ttttaattga
      121 ttggttatgg tgtaaatatt acatagcttt aactgataat ctgattactt tatttcgtgt
      181 gtctatgatg atgatgatag ttacagaacc gacgaactag tctgtacccg atcaacaccg
      241 agacccgtgg cgtcttcgac ctcaatggcg tctggaactt caagctggac tacgggaaag
      301 gactggaaga gaagtggtac gaaagcaagc tgaccgacac tattagtatg gccgtcccaa
      361 gcagttacaa tgacattggc gtgaccaagg aaatccgcaa ccatatcgga tatgtctggt
      421 acgaacgtga gttcacggtg ccggcctatc tgaaggatca gcgtatcgtg ctccgcttcg
      481 gctctgcaac tcacaaagca attgtctatg tcaatggtga gctggtcgtg gagcacaagg
      541 gcggattcct gccattcgaa gcggaaatca acaactcgct gcgtgatggc atgaatcgcg
      601 tcaccgtcgc cgtggacaac atcctcgacg atagcaccct cccggtgggg ctgtacagcg
      661 agcgccacga agagggcctc ggaaaagtca ttcgtaacaa gccgaacttc gacttcttca
      721 actatgcagg cctgcaccgt ccggtgaaaa tctacacgac cccgtttacg tacgtcgagg
      781 acatctcggt tgtgaccgac ttcaatggcc caaccgggac tgtgacctat acggtggact
      841 ttcaaggcaa agccgagacc gtgaaagtgt cggtcgtgga tgaggaaggc aaagtggtcg
      901 caagcaccga gggcctgagc ggtaacgtgg agattccgaa tgtcatcctc tgggaaccac
      961 tgaacacgta tctctaccag atcaaagtgg aactggtgaa cgacggactg accatcgatg
     1021 tctatgaaga gccgttcggc gtgcggaccg tggaagtcaa cgacggcaag ttcctcatca
     1081 acaacaaacc gttctacttc aagggctttg gcaaacatga ggacactcct atcaacggcc
     1141 gtggctttaa cgaagcgagc aatgtgatgg atttcaatat cctcaaatgg atcggcgcca
     1201 acagcttccg gaccgcacac tatccgtact ctgaagagtt gatgcgtctt gcggatcgcg
     1261 agggtctggt cgtgatcgac gagactccgg cagttggcgt gcacctcaac ttcatggcca
     1321 ccacgggact cggcgaaggc agcgagcgcg tcagtacctg ggagaagatt cggacgtttg
     1381 agcaccatca agacgttctc cgtgaactgg tgtctcgtga caagaaccat ccaagcgtcg
     1441 tgatgtggag catcgccaac gaggcggcga ctgaggaaga gggcgcgtac gagtacttca
     1501 agccgttggt ggagctgacc aaggaactcg acccacagaa gcgtccggtc acgatcgtgc
     1561 tgtttgtgat ggctaccccg gagacggaca aagtcgccga actgattgac gtcatcgcgc
     1621 tcaatcgcta taacggatgg tacttcgatg gcggtgatct cgaagcggcc aaagtccatc
     1681 tccgccagga atttcacgcg tggaacaagc gttgcccagg aaagccgatc atgatcactg
     1741 agtacggcgc agacaccgtt gcgggctttc acgacattga tccagtgatg ttcaccgagg
     1801 aatatcaagt cgagtactac caggcgaacc acgtcgtgtt cgatgagttt gagaacttcg
     1861 tgggtgagca agcgtggaac ttcgcggact tcgcgacctc tcagggcgtg atgcgcgtcc
     1921 aaggaaacaa gaagggcgtg ttcactcgtg accgcaagcc gaagctcgcc gcgcacgtct
     1981 ttcgcgagcg ctggaccaac attccagatt tcggctacaa gaacgctagc catcaccatc
     2041 accatcacgt gtgaattggt gaccagctcg aatttccccg atcgttcaaa catttggcaa
     2101 taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg
     2161 ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg
     2221 gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag
     2281 cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaatta
     2341 aactatcagt gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt
     2401 agaataacgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc
     2461 atgccaacca cagggttccc ctcgggatca aagtactttg atccaacccc tccgctgcta
     2521 tagtgcagtc ggcttctgac gttcagtgca gccgtcttct gaaaacgaca tgtcgcacaa
     2581 gtcctaagtt acgcgacagg ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg
     2641 ttttagtcgc ataaagtaga atacttgcga ctagaaccgg agacattacg ccatgaacaa
     2701 gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc accgacgacc aggacttgac
     2761 caaccaacgg gccgaactgc acgcggccgg ctgcaccaag ctgttttccg agaagatcac
     2821 cggcaccagg cgcgaccgcc cggagctggc caggatgctt gaccacctac gccctggcga
     2881 cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc tactggacat
     2941 tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga
     3001 caccaccacg ccggccggcc gcatggtgtt gaccgtgttc gccggcattg ccgagttcga
     3061 gcgttcccta atcatcgacc gcacccggag cgggcgcgag gccgccaagg cccgaggcgt
     3121 gaagtttggc ccccgcccta ccctcacccc ggcacagatc gcgcacgccc gcgagctgat
     3181 cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac
     3241 cctgtaccgc gcacttgagc gcagcgagga agtgacgccc accgaggcca ggcggcgcgg
     3301 tgccttccgt gaggacgcat tgaccgaggc cgacgccctg gcggccgccg agaatgaacg
     3361 ccaagaggaa caagcatgaa accgcaccag gacggccagg acgaaccgtt tttcattacc
     3421 gaagagatcg aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacgtc
     3481 tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct ggcggcctgg
     3541 ccggccagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt
     3601 gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa
     3661 atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca
     3721 agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc gatgttctgt
     3781 tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac
     3841 cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag gccatcggcc
     3901 ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct gtgtccgcga
     3961 tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac atatgggcca
     4021 ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga aggctacaag
     4081 cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg
     4141 cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc gtgagctacc
     4201 caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc gacgctgccc
     4261 gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt aatgaggtaa
     4321 agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg cacgcagcag
     4381 caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg tcaactttca
     4441 gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat
     4501 taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca aatgaataaa
     4561 tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa caaccaggca
     4621 ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc gtaagcggct
     4681 gggttgtctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa tcggcgtgac
     4741 ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag
     4801 aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc acgccccggt
     4861 gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc
     4921 ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt tttcgttccg
     4981 atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc cgttttccgt
     5041 ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct acgagcttcc agacgggcac
     5101 gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt gggattacga cctggtactg
     5161 atggcggttt cccatctaac cgaatccatg aaccgatacc gggaagggaa gggagacaag
     5221 cccggccgcg tgttccgtcc acacgttgcg gacgtactca agttctgccg gcgagccgat
     5281 ggcggaaagc agaaagacga cctggtagaa acctgcattc ggttaaacac cacgcacgtt
     5341 gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa
     5401 gccttgatta gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag
     5461 atcgagctag ctgattggat gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg
     5521 acggttcacc ccgattactt tttgatcgat cccggcatcg gccgttttct ctaccgcctg
     5581 gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat ctacgaacgc
     5641 agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct gatcgggtca
     5701 aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc gatcctagtc
     5761 atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg tacggagcag
     5821 atgctagggc aaattgccct agcaggggaa aaaggtcgaa aaggtctctt tcctgtggat
     5881 agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta cattgggaac
     5941 ccaaagccgt acattgggaa ccggtcacac atgtaagtga ctgatataaa agagaaaaaa
     6001 ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc
     6061 tgtgcataac tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg
     6121 tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc tggccgctca
     6181 aaaatggctg gcctacggcc aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca
     6241 ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga
     6301 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     6361 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
     6421 gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag
     6481 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     6541 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     6601 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     6661 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     6721 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     6781 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     6841 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     6901 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     6961 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     7021 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     7081 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     7141 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     7201 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     7261 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     7321 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     7381 cgttaaggga ttttggtcat gcattctagg tactaaaaca attcatccag taaaatataa
     7441 tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac
     7501 tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc ataccacttg
     7561 tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc tttcacaaag
     7621 atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg cttttccgtc
     7681 tttaaaaaat catacagctc gcgcggatct ttaaatggag tgtcttcttc ccagttttcg
     7741 caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa gcggctgtct
     7801 aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct gatgcactcc
     7861 gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc cgagcaaagg
     7921 acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc aaagtgcagg
     7981 acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc ccgttccaca
     8041 tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt ttcattttct
     8101 cccaccagct tatatacctt agcaggagac attccttccg tatcttttac gcagcggtat
     8161 ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta ttatttcctt
     8221 cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag acgaactcca
     8281 attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt
     8341 tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaaccg cggtgatcac
     8401 aggcagcaac gctctgtcat cgttacaatc aacatgctac cctccgcgag atcatccgtg
     8461 tttcaaaccc ggcagcttag ttgccgttct tccgaatagc atcggtaaca tgagcaaagt
     8521 ctgccgcctt acaacggctc tcccgctgac gccgtcccgg actgatgggc tgcctgtatc
     8581 gagtggtgat tttgtgccga gctgccggtc ggggagctgt tggctggctg gtggcaggat
     8641 atattgtggt gtaaacaaat tgacgcttag acaacttaat aacacattgc ggacgttttt
     8701 aatgtactga attaacgccg aattaattcg ggggatctgg attttagtac tggattttgg
     8761 ttttaggaat tagaaatttt attgatagaa gtattttaca aatacaaata catactaagg
     8821 gtttcttata tgctcaacac atgagcgaaa ccctatagga accctaattc ccttatctgg
     8881 gaactactca cacattatta tggagaaact cgagcttgtc gatcgacaga tccggtcggc
     8941 atctactcta tttctttgcc ctcggacgag tgctggggcg tcggtttcca ctatcggcga
     9001 gtacttctac acagccatcg gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc
     9061 cgacagtccc ggctccggat cggacgattg cgtcgcatcg accctgcgcc caagctgcat
     9121 catcgaaatt gccgtcaacc aagctctgat agagttggtc aagaccaatg cggagcatat
     9181 acgcccggag tcgtggcgat cctgcaagct ccggatgcct ccgctcgaag tagcgcgtct
     9241 gctgctccat acaagccaac cacggcctcc agaagaagat gttggcgacc tcgtattggg
     9301 aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca ttgtccgtca
     9361 ggacattgtt ggagccgaaa tccgcgtgca cgaggtgccg gacttcgggg cagtcctcgg
     9421 cccaaagcat cagctcatcg agagcctgcg cgacggacgc actgacggtg tcgtccatca
     9481 cagtttgcca gtgatacaca tggggatcag caatcgcgca tatgaaatca cgccatgtag
     9541 tgtattgacc gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg ctaagatcgg
     9601 ccgcagcgat cgcatccata gcctccgcga ccggttgtag aacagcgggc agttcggttt
     9661 caggcaggtc ttgcaacgtg acaccctgtg cacggcggga gatgcaatag gtcaggctct
     9721 cgctaaactc cccaatgtca agcacttccg gaatcgggag cgcggccgat gcaaagtgcc
     9781 gataaacata acgatctttg tagaaaccat cggcgcagct atttacccgc aggacatatc
     9841 cacgccctcc tacatcgaag ctgaaagcac gagattcttc gccctccgag agctgcatca
     9901 ggtcggagac gctgtcgaac ttttcgatca gaaacttctc gacagacgtc gcggtgagtt
     9961 caggcttttt catatctcat tgccccccgg gatctgcgaa agctcgagag agatagattt
    10021 gtagagagag actggtgatt tcagcgtgtc ctctccaaat gaaatgaact tccttatata
    10081 gaggaaggtc ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat
    10141 cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc
    10201 tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttga acgatagcct
    10261 ttcctttatc gcaatgatgg catttgtagg tgccaccttc cttttctact gtccttttga
    10321 tgaagtgaca gatagctggg caatggaatc cgaggaggtt tcccgatatt accctttgtt
    10381 gaaaagtctc aatagccctt tggtcttctg agactgtatc tttgatattc ttggagtaga
    10441 cgagagtgtc gtgctccacc atgttatcac atcaatccac ttgctttgaa gacgtggttg
    10501 gaacgtcttc tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt
    10561 cggcagaggc atcttgaacg atagcctttc ctttatcgca atgatggcat ttgtaggtgc
    10621 caccttcctt ttctactgtc cttttgatga agtgacagat agctgggcaa tggaatccga
    10681 ggaggtttcc cgatattacc ctttgttgaa aagtctcaat agccctttgg tcttctgaga
    10741 ctgtatcttt gatattcttg gagtagacga gagtgtcgtg ctccaccatg ttggcaagct
    10801 gctctagcca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
    10861 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
    10921 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
    10981 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgaattcga
    11041 gctcggtacc cggggatcct ctagagtcga cctgcaggca tgcaagcttg gcactggccg
    11101 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
    11161 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
    11221 aacagttgcg cagcctgaat ggcgaatgct agagcagctt gagcttggat cagattgtcg
    11281 tttcccgcct tcagtttagc ttcatggagt caaagattca aatagaggac ctaacagaac
    11341 tcgccgtaaa gactggcgaa cagttcatac agagtctctt acgactcaat gacaagaaga
    11401 aaatcttcgt caacatggtg gagcacgaca cacttgtcta ctccaaaaat atcaaagata
    11461 cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata tccggaaacc
    11521 tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg gaaaaggaag
    11581 gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa gatgcctctg
    11641 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
    11701 ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg
    11761 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
    11821 tggagagaac acgggggact cttgac
//

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