| 质粒名称: | pAAV-MCS2 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 46954 |
| 存储实验室: | Steve Jackson |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS |
| 载体出品公司: | Agilent Technologies |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 4650 bp |
| 完整质粒大小: | -- |
| 载体修饰: | 用NotI-bsp12oi酶切Ku80 cDNA的1345bp片段，并将其作为连接子克隆到NotI酶切的pAAV-MCS中。 |
| 载体类型: | AAV |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pAAV-MCS2 was a gift from Steve Jackson (Addgene plasmid # 46954 ; http://n2t.net/addgene:46954 ; RRID:Addgene_46954) |
| 参考文献部分: | A new method for high-resolution imaging of Ku foci to decipher mechanisms of DNA double-strand break repair. Britton S, Coates J, Jackson SP. J Cell Biol. 2013 Jul 29. 10.1083/jcb.201303073 PubMed 23897892 |
买家导航
pAAVMCS2载体基本信息
pAAVMCS2载体图谱质粒图谱和多克隆位点信息
pAAVMCS2载体简介
pAAVMCS2载体序列
LOCUS pAAV-MCS2 4263 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Cloning plasmid for the generation of Knock-In in human cells using
rAAV..
ACCESSION .
VERSION .
KEYWORDS pAAV-MCS2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4263)
AUTHORS Britton S, Coates J, Jackson SP
TITLE A new method for high-resolution imaging of Ku foci to decipher
mechanisms of DNA double-strand break repair.
JOURNAL J Cell Biol. 2013 Jul 29.
PUBMED 23897892
REFERENCE 2 (bases 1 to 4263)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4263
/organism="synthetic DNA construct"
/mol_type="other DNA"
repeat_region 675..815
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
repeat_region 675..804
/label=AAV2 ITR
rep_origin complement(877..1465)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(957..976)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(1636..2496)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 2259..2278
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(2497..2601)
/gene="bla"
/label=AmpR promoter
primer_bind 2669..2687
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(2725..2747)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 2847..2866
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
rep_origin complement(2883..3338)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3020..3041)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 3232..3251
/label=F1ori-R
/note="F1 origin, reverse primer"
repeat_region 3413..3553
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
repeat_region 3424..3553
/label=AAV2 ITR
ORIGIN
1 aagaaaattg agaggcattc cattcactgg ccctgccgac tgaccattgg ctccaatttg
61 tctataagga ttgcagccta taaatcgatt ctacaggaga gagttaaaaa gacttggaca
121 gttgtggatg caaaaaccct aaaaaaagaa gatatacaaa aagaaacagt ttattgctta
181 aatgatgatg atgaaactga agttttaaaa gaggatatta ttcaagggtt ccgctatgga
241 agtgatatag ttcctttctc taaagtggat gaggaacaaa tgaaatataa atcggagggg
301 aagtgcttct ctgttttggg attttgtaaa tcttctcagg ttcagagaag attcttcatg
361 ggaaatcaag ttctaaaggt ctttgcagca agagatgatg aggcagctgc agttgcactt
421 tcctccctga ttcatgcttt ggatgactta gacatggtgg ccatagttcg atacgcttat
481 gacaaaagag ctaatcctca agtcggcgtg gcttttcctc atatcaagca taactatgag
541 tgtttagtgt atgtgcagct gcctttcatg gaagacttgc ggcaatacat gttttcatcc
601 ttgaaaaaca gtaagaaata tgctcccacc gaggcacagt tgaatgctgt tgatgggatc
661 cacgcgtggg ccgcaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg
721 ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc
781 ctcagtgagc gagcgagcgc gcagctgcct gcaggacatg tgagcaaaag gccagcaaaa
841 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
901 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
961 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
1021 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
1081 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
1141 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
1201 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
1261 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
1321 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
1381 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
1441 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
1501 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
1561 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
1621 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
1681 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
1741 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
1801 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
1861 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
1921 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
1981 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
2041 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
2101 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
2161 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
2221 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
2281 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
2341 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
2401 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
2461 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
2521 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
2581 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
2641 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
2701 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
2761 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
2821 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
2881 taaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
2941 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagcccga
3001 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
3061 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
3121 caaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
3181 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
3241 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
3301 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtac tatggttgct ttgacgtatg
3361 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg cccctgcagg
3421 cagctgcgcg ctcgctcgct cactgaggcc gcccgggcaa agcccgggcg tcgggcgacc
3481 tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag agggagtggc caactccatc
3541 actaggggtt cctgcggccg cgctagcgaa ttcgcagctg ttgtgctgtg tatggacgtg
3601 ggctttacca tgagtaactc cattcctggt atagaatccc catttgaaca agcaaagaag
3661 gtgataacca tgtttgtaca gcgacaggtg tttgctgaga acaaggatga gattgcttta
3721 gtcctgtttg gtacagatgg cactgacaat cccctttctg gtggggatca gtatcagaac
3781 atcacagtgc acagacatct gatgctacca gattttgatt tgctggagga cattgaaagc
3841 aaaatccaac caggttctca acaggctgac ttcctggatg cactaatcgt gagcatggat
3901 gtgattcaac atgaaacaat aggaaagaag tttgagaaga ggcatattga aatattcact
3961 gacctcagca gccgattcag caaaagtcag ctggatatta taattcatag cttgaagaaa
4021 tgtgacatct ccctgcaatt cttcttgcct ttctcacttg gcaaggaaga tggaagtggg
4081 gacagaggag atggcccctt tcgcttaggt ggccatgggc cttcctttcc actaaaagga
4141 attaccgaac agcaaaaaga aggtcttgag atagtgaaaa tggtgatgat atctttagaa
4201 ggtgaagatg ggttggatga aatttattca ttcagtgaga gtctgagaaa actgtgcgtc
4261 ttc
//
