pWPXL

价格:800元

联系方式:I47-825O-882O

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载体基本信息

出品公司: --
载体名称: pWPXL
质粒类型: 哺乳动物细胞慢病毒表达载体
高拷贝/低拷贝: 高拷贝
启动子: EF-1 alpha
克隆方法: 多克隆位点,限制性内切酶
载体大小: 10510 bp
5' 测序引物及序列: EF-1a Forward:   TCAAGCCTCAGACAGTGGTTC
3' 测序引物及序列: EGFP-N: CGTCGCCGTCCAGCTCGACCAG
载体标签: C-EGFP
载体抗性: 氨苄
备注: pWPXL载体是用来进行稳定型转基因表达。pWPXL载体含有EF-1alpha启动子和内含子,这使得基因能够高效表达。载体3‘ LTR区的LoxP被克隆至目的细胞的5’ LTR反转录区。这 使得之前在Cre酶作用下插入的基因得以移除出去。载体使用Stbl3感受态细胞扩增质粒,可以减少载体基因重组。
产品目录号: --
稳定性: /
组成型: --
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

pWPXL载体图谱


载体简介

载体序列

LOCUS       pWPXL	10510 bp 	DNA     circular	SYN
DEFINITION  pWPXL
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..10510
                     /organism="pWPXL"
                     /mol_type="other DNA"
     misc_feature    455..635
                     /label="truncHIV-1_3_LTR"
     misc_feature    455..635
                     /label="HIV-1_5_LTR"
     misc_feature    746..790
                     /label="HIV-1_psi_pack"
     CDS             1178..2035
                     /label="ORF frame 2"
     misc_feature    1300..1533
                     /label="RRE"
     CDS             complement(1997..3037)
                     /label="ORF frame 1"
     promoter        2127..3297
                     /label="EF1a_promoter"
     misc_feature    3245..3265
                     /label="EF1a_fwd_primer"
     misc_feature    3349..3364
                     /label="cPPT"
     CDS             3544..4281
                     /label="ORF frame 1"
     gene            3547..4260
                     /label="EGFP"
                     /gene="EGFP"
     misc_feature    complement(3589..3610)
                     /label="EGFP_N_primer"
     misc_feature    3727..3756
                     /label="Y66 (EGFP)"
     misc_feature    4197..4218
                     /label="EGFP_C_primer"
     misc_feature    4342..4929
                     /label="WPRE"
     CDS             complement(4427..4930)
                     /label="ORF frame 1"
     CDS             4430..4990
                     /label="ORF frame 2"
     misc_feature    5000..5015
                     /label="cPPT"
     misc_feature    5000..5021
                     /label="U3PPT"
     misc_feature    5072..5105
                     /label="loxP"
     misc_feature    5125..5305
                     /label="truncHIV-1_3_LTR"
     misc_feature    5125..5305
                     /label="HIV-1_5_LTR"
     misc_feature    complement(5331..5348)
                     /label="Sp6_primer"
     gene            5586..5849
                     /label="tet (300 - 563)"
                     /gene="tet (300 - 563)"
     misc_feature    complement(5638..5657)
                     /label="pBRrevBam_primer"
     misc_feature    5990..6012
                     /label="pGEX_3_primer"
     promoter        6171..6199
                     /label="AmpR_promoter"
     gene            6241..7101
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             6241..7101
                     /label="ORF frame 1"
     rep_origin      7256..7875
                     /label="pBR322_origin"
     misc_feature    complement(8101..8121)
                     /label="pBABE_3_primer"
     misc_feature    complement(8107..8560)
                     /label="SV40_enhancer"
     promoter        8119..8387
                     /label="SV40_promoter"
     rep_origin      8286..8363
                     /label="SV40_origin"
     misc_feature    8348..8367
                     /label="SV40pro_F_primer"
     misc_feature    9568..9583
                     /label="SV40_int"
     misc_feature    9589..9636
                     /label="SV40_3_splice"
     CDS             9713..10186
                     /label="ORF frame 2"
     misc_feature    9764..9784
                     /label="SV40 NLS"
     misc_feature    9770..9784
                     /label="NLS"
     terminator      10212..10343
                     /label="SV40_PA_terminator"
     misc_feature    10300..10319
                     /label="EBV_rev_primer"
ORIGIN
    1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
   61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
  121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
  181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
  241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
  301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
  361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
  421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
  481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
  541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
  601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
  661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
  721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
  781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
  841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
  901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
  961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
 1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
 1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
 1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
 1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
 1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
 1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
 1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
 1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
 1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
 1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
 1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
 1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
 1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
 1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
 1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
 1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
 1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgatgtcgac gataagcttt
 2041 gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt
 2101 cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
 2161 agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg
 2221 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
 2281 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
 2341 cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg
 2401 cccttgcgtg ccttgaatta cttccactgg ctgcagtacg tgattcttga tcccgagctt
 2461 cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt
 2521 gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt
 2581 cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct
 2641 gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt
 2701 atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg
 2761 gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc
 2821 cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg
 2881 ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca
 2941 gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa
 3001 aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg
 3061 ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg
 3121 gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca
 3181 gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc
 3241 attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgagga
 3301 atttcgacat ttaaatttaa ttaatctcga cggtatcggt taacttttaa aagaaaaggg
 3361 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
 3421 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttccgatcac gagactagcc
 3481 tcgaggttta aactacggga tccaggccta agcttacgcg tcctagcgct accggtcgcc
 3541 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
 3601 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
 3661 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
 3721 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
 3781 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
 3841 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
 3901 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
 3961 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
 4021 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
 4081 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
 4141 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
 4201 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
 4261 tccggactca gatctcgata gctagtagct agctagctag tcgagctcaa cttcgaattc
 4321 ccgggactag tcatatgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
 4381 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
 4441 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
 4501 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
 4561 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
 4621 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
 4681 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
 4741 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
 4801 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
 4861 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
 4921 ctccccgcat cggtacgtat ggccaggtac ctttaagacc aatgacttac aaggcagctg
 4981 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
 5041 gaagacaaga tgggatcaat tcaccatggg aataacttcg tatagcatac attatacgaa
 5101 gttatgctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
 5161 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
 5221 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
 5281 tagtcagtgt ggaaaatctc tagcagcatc tagaattaat tccgtgtatt ctatagtgtc
 5341 acctaaatcg tatgtgtatg atacataagg ttatgtatta attgtagccg cgttctaacg
 5401 acaatatgta caagcctaat tgtgtagcat ctggcttact gaagcagacc ctatcatctc
 5461 tctcgtaaac tgccgtcaga gtcggtttgg ttggacgaac cttctgagtt tctggtaacg
 5521 ccgtcccgca cccggaaatg gtcagcgaac caatcagcag ggtcatcgct agccagatcc
 5581 tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct
 5641 atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt
 5701 gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct
 5761 tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct
 5821 tcctaatgca ggagtcgcat aagggagagc gtcgaatggt gcactctcag tacaatctgc
 5881 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
 5941 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
 6001 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
 6061 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
 6121 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
 6181 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
 6241 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
 6301 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
 6361 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
 6421 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
 6481 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
 6541 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
 6601 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
 6661 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
 6721 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
 6781 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
 6841 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
 6901 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
 6961 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
 7021 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
 7081 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
 7141 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
 7201 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
 7261 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
 7321 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
 7381 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
 7441 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
 7501 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
 7561 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
 7621 gagcgaacga cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg
 7681 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
 7741 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
 7801 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
 7861 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
 7921 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
 7981 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
 8041 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgt
 8101 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
 8161 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
 8221 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
 8281 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
 8341 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
 8401 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tggacacaag acaggcttgc
 8461 gagatatgtt tgagaatacc actttatccc gcgtcaggga gaggcagtgc gtaaaaagac
 8521 gcggactcat gtgaaatact ggtttttagt gcgccagatc tctataatct cgcgcaacct
 8581 attttcccct cgaacacttt ttaagccgta gataaacagg ctgggacact tcacatgagc
 8641 gaaaaataca tcgtcacctg ggacatgttg cagatccatg cacgtaaact cgcaagccga
 8701 ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa gccgtggcgg tctgtaccgg
 8761 gtgcgttact ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt atttccagct
 8821 acgatcacga caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc gatggcgaag
 8881 gcttcatcgt tattgatgac ctggtggata ccggtggtac tgcggttgcg attcgtgaaa
 8941 tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt ccgctggttg
 9001 atgactatgt tgttgatatc ccgcaagata cctggattga acagccgtgg gatatgggcg
 9061 tcgtattcgt cccgccaatc tccggtcgct aatcttttca acgcctggca ctgccgggcg
 9121 ttgttctttt taacttcagg cgggttacaa tagtttccag taagtattct ggaggctgca
 9181 tccatgacac aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa acatcctgaa
 9241 acctcgacgc tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca gtcggccctt
 9301 gatggtaaaa ccatccctca ctggtatcgc atgattaacc gtctgatgtg gatctggcgc
 9361 ggcattgacc cacgcgaaat cctcgacgtc caggcacgta ttgtgatgag cgatgccgaa
 9421 cgtaccgacg atgatttata cgatacggtg attggctacc gtggcggcaa ctggatttat
 9481 gagtgggccc cggatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa
 9541 actacctaca gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt
 9601 taaactactg attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg
 9661 ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc
 9721 tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa
 9781 ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt
 9841 tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct
 9901 atacaagaaa attatggaaa aatattctgt aacctttata agtaggcata acagttataa
 9961 tcataacata ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta
10021 tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt
10081 gatgtatagt gccttgacta gagatcataa tcagccatac cacatttgta gaggttttac
10141 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
10201 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
10261 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
10321 atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac
10381 ttcccacccc ataccctatt accactgcca attacctagt ggtttcattt actctaaacc
10441 tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca
10501 aacttagtag
//

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