| 质粒名称: | pcDNA3.1-Peredox-mCherry |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 32383 |
| 存储实验室: | Gary Yellen |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 载体出品公司: | InVitrogen |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 5400 bp |
| 载体类型: | 哺乳细胞表达 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Peredox-mCherry |
| 插入大小: | 2800 bp |
| 启动子 | CMV |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | XbaI(未破坏) |
| 3' 克隆位点: | XhoI (克隆期间被破坏) |
| 5' 测序引物 | T7 |
| 3' 测序引物 | mCherry-R |
|
文献引用方法 |
|
| 材料和方法部分: | pcDNA3.1-Peredox-mCherry was a gift from Gary Yellen (Addgene plasmid # 32383) |
| 参考文献部分: | Imaging Cytosolic NADH-NAD(+) Redox State with a Genetically Encoded Fluorescent Biosensor. Hung YP, Albeck JG, Tantama M, Yellen G. Cell Metab. 2011 Oct 5;14(4):545-54. 10.1016/j.cmet.2011.08.012 PubMed 21982714 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pcDNA3.1-Peredox-mCherry 8125 bp DNA SYN
DEFINITION pcDNA3.1-Peredox-mCherry
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..8125
/organism="pcDNA3.1-Peredox-mCherry"
/mol_type="other DNA"
CDS complement(28..762)
/label="ORF frame 2"
promoter 66..84
/label="T7_promoter"
CDS 121..2889
/label="ORF frame 1"
CDS complement(934..1485)
/label="ORF frame 2"
CDS complement(1683..2246)
/label="ORF frame 3"
gene 2182..2886
/label="mCherry"
/gene="mCherry"
misc_feature 2359..2412
/label="Q66M (mCherry)"
misc_feature complement(2922..2939)
/label="BGH_rev_primer"
terminator 2925..3152
/label="bGH_PA_terminator"
rep_origin 3215..3521
/label="f1_origin"
misc_feature complement(3635..3655)
/label="pBABE_3_primer"
misc_feature complement(3641..3856)
/label="SV40_enhancer"
promoter 3653..3921
/label="SV40_promoter"
rep_origin 3820..3897
/label="SV40_origin"
misc_feature 3882..3901
/label="SV40pro_F_primer"
CDS 4036..4830
/label="ORF frame 1"
gene 4039..4827
/label="NeoR/KanR"
/gene="NeoR/KanR"
CDS complement(4345..4881)
/label="ORF frame 2"
terminator 5007..5126
/label="SV40_PA_terminator"
misc_feature 5095..5114
/label="EBV_rev_primer"
promoter complement(5170..5188)
/label="M13_reverse_primer"
misc_feature complement(5187..5209)
/label="M13_pUC_rev_primer"
promoter complement(5223..5252)
/label="lac_promoter"
gene complement(6332..7192)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(6332..7192)
/label="ORF frame 1"
promoter complement(7234..7262)
/label="AmpR_promoter"
promoter 7564..55
/label="CMV_immearly_promoter"
misc_feature 7643..7930
/label="CAG_enhancer"
misc_feature 8097..8117
/label="CMV_fwd_primer"
ORIGIN
1 gaggtctata taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc
61 gaaattaata cgactcacta tagggagacc caagctggct agctctagat tgggcccacc
121 atggttgcta gcaaagttcc tgaagcagcc atttccagac tgattactta cctgagaatt
181 ctggaagagc tggaagccca aggtgtacac cgcaccgcct ccgagcaact gggcgagctg
241 gcccaggtca ccgccttcca ggttgataag gacctgtcct acttcggctc ctacggcact
301 gacggcgttg gctacactgt accggtcctc aagagagaac tgcgccatat cctcggtctg
361 aaccgcaaat ggggcctgtg catcgtgggg atgggccgcc tgggatccgc tcttgctgat
421 tggcctggtt ttggcgagag cttcgagctg cgcggtttct tcgatgttga cccaggtatg
481 gttggtcggc cggttcgcgg tggtgttatc gaacacgttg atctgctgcc ccagcgcgta
541 cctggtcgca tcgaaatcgc tctgctgacg gttccacgcg aggcagcaca gaaggctgcc
601 gacctgctgg ttgccgctgg catcaagggc atcctgaact tcgctccagt tgtactggag
661 gttcccaagg aagtggctgt tgagaacgtt gacatcctgg ccggtctgac gcgtctgagc
721 tttgccattc tgaaccccac gtggagcgca gcaggtgggc atggtttcac tgctcacaac
781 gtgtatatca tggctgacaa acagaagaac ggtatcaagg ccaacttcaa gatccgccac
841 aacatcgaag acggcggcgt tcagctggct gatcactacc agcagaacac cccaatcggc
901 gacggccctg ttctgctgcc tgacaaccac tacttaagca tccagtctaa actgtccaaa
961 gacccaaacg agaagcgcga ccacatggtt ctgctggaat ttgttaccgc cgctggtatc
1021 acccacggca tggacgaact gtacaagggc ggcaccgggg gatctatggt ttccaaaggc
1081 gaggaactgt tcactggtgt tgttcctatc ctggttgaac tggacggcga cgttaacggc
1141 cacaaattca gcgttagcgg ggaaggcgaa ggagacgcta cctacggcaa gctgactctg
1201 aagttcatct gcaccactgg caagctgcct gttccttggc ctaccctggt tactaccttc
1261 tcctatggtg ttatggtttt cgccagatat cctgaccaca tgaagcagca cgacttcttc
1321 aagagcgcca tgcctgaggg ctacgttcag gaacgcacca tctttttcaa ggacgacggc
1381 aactataaga cacgcgctga ggttaagttc gagggcgaca ctctggttaa ccgcatcgag
1441 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg gccataagct tgaatataac
1501 accaaggtac ctgaagccgc catcagccgc ctcatcactt acctccgcat cctggaggag
1561 ctcgaagccc aaggcgttca ccgcactgca agcgagcagc tgggcgaact ggcacaggtt
1621 acagcattcc aggttgacga agatctgtcc tatttcggtt cctacggcac cgacggtgtt
1681 ggctacacag tccctgtcct gaagcgcgag ctgcgccaca tcctgggcct gaaccgcaag
1741 tggggcttat gcatcgttgg catgggccgc ctcggtagtg cactggcaga ctggccgggc
1801 tttggtgaat ctttcgaact gcgcggcttc ttcgacgttg atccaggcat ggttgggcgc
1861 cctgttcgcg gtggcgttat cgagcatgtt gacctcctgc cacagcgcgt cccgggtcgc
1921 atcgagatcg ccctgctgac cgttcctcgc gaagctgcac agaaggcagc cgacttacta
1981 gtcgccgctg gtatcaaggg aattctgaac ttcgcccctg ttgttctgga ggttcctaag
2041 gaagttgctg ttgaaaacgt tgatttcctg gccggcctga cccgcctgag cttcgcaatc
2101 ctgaatccta agtggcgcga agaaatgatg ggttccggaa ctgggggcaa cgcctctgac
2161 ggtggtgggt ctggtggtat ggtgagcaag ggcgaggagg ataacatggc catcatcaag
2221 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
2281 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
2341 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
2401 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
2461 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
2521 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
2581 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
2641 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
2701 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
2761 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
2821 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgaactg
2881 tacaagtgag tcgagaagct taagtttaaa ccgctgatca gcctcgactg tgccttctag
2941 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
3001 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
3061 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
3121 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
3181 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
3241 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
3301 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
3361 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
3421 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
3481 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
3541 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
3601 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
3661 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
3721 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
3781 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
3841 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
3901 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
3961 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca agagacagga
4021 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
4081 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
4141 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
4201 gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
4261 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
4321 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
4381 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
4441 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
4501 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
4561 gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
4621 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
4681 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
4741 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
4801 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc
4861 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt
4921 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
4981 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
5041 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
5101 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
5161 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
5221 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
5281 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
5341 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
5401 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
5461 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
5521 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
5581 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
5641 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
5701 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
5761 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
5821 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
5881 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
5941 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
6001 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
6061 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtttttttg
6121 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
6181 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
6241 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
6301 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
6361 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
6421 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
6481 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
6541 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
6601 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
6661 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
6721 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
6781 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
6841 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
6901 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
6961 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
7021 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
7081 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
7141 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
7201 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
7261 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
7321 ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg
7381 ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga
7441 gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa
7501 gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg
7561 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
7621 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
7681 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
7741 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
7801 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
7861 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
7921 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
7981 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
8041 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
8101 aatgggcggt aggcgtgtac ggtgg
//
