| 出品公司: | -- |
|---|---|
| 载体名称: | pWB980 |
| 质粒类型: | 枯草芽孢杆菌表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | P43 |
| 载体大小: | 3772 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 卡那霉素 |
| 筛选标记: | -- |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 常规细胞系（293、CV-1、CHO等） |
| 备注: | |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pWB980 3787 bp DNA circular SYN 13-JUL-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3787)
AUTHORS Biofeng
TITLE Direct Submission
JOURNAL Exported Jul 13, 2023 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..3787
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS 309..1313
/codon_start=1
/gene="repB"
/product="RepB replication protein"
/label=repB
/note="from Enterococcus faecalis plasmid pAM-alpha-1"
/db_xref="GI:22652809"
/protein_id="AAN03827.1"
/translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY
FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI
QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV
GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN
VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS
YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"
ORIGIN
1 agaacctaaa aagaacgaat ttgaactaac tcataaccga gaggtaaaaa aagaacgaag
61 tcgagatcag ggaatgagtt tataaaataa aaaaagcacc tgaaaaggtg tctttttttg
121 atggttttga acttgttctt tcttatcttg atacatatag aaataacgtc atttttattt
181 tagttgctga aaggtgcgtt gaagtgttgg tatgtatgtg ttttaaagta ttgaaaaccc
241 ttaaaattgg ttgcacagaa aaaccccatc tgttaaagtt ataagtgact aaacaaataa
301 ctaaatagat gggggtttct tttaatatta tgtgtcctaa tagtagcatt tattcagatg
361 aaaaatcaag ggttttagtg gacaagacaa aaagtggaaa agtgagacca tggagagaaa
421 agaaaatcgc taatgttgat tactttgaac ttctgcatat tcttgaattt aaaaaggctg
481 aaagagtaaa agattgtgct gaaatattag agtataaaca aaatcgtgaa acaggcgaaa
541 gaaagttgta tcgagtgtgg ttttgtaaat ccaggctttg tccaatgtgc aactggagga
601 gagcaatgaa acatggcatt cagtcacaaa aggttgttgc tgaagttatt aaacaaaagc
661 caacagttcg ttggttgttt ctcacattaa cagttaaaaa tgtttatgat ggcgaagaat
721 taaataagag tttgtcagat atggctcaag gatttcgccg aatgatgcaa tataaaaaaa
781 ttaataaaaa tcttgttggt tttatgcgtg caacggaagt gacaataaat aataaagata
841 attcttataa tcagcacatg catgtattgg tatgtgtgga accaacttat tttaagaata
901 cagaaaacta cgtgaatcaa aaacaatgga ttcaattttg gaaaaaggca atgaaattag
961 actatgatcc aaatgtaaaa gttcaaatga ttcgaccgaa aaataaatat aaatcggata
1021 tacaatcggc aattgacgaa actgcaaaat atcctgtaaa ggatacggat tttatgaccg
1081 atgatgaaga aaagaatttg aaacgtttgt ctgatttgga ggaaggttta caccgtaaaa
1141 ggttaatctc ctatggtggt ttgttaaaag aaatacataa aaaattaaac cttgatgaca
1201 cagaagaagg cgatttgatt catacagatg atgacgaaaa agccgatgaa gatggatttt
1261 ctattattgc aatgtggaat tgggaacgga aaaattattt tattaaagag tagttcaaca
1321 aacgggccag tttgttgaag attagatgct ataattgtta ttaaaaggat tgaaggatgc
1381 ttaggaagac gagttattaa tagctgaata agaacggtgc tctccaaata ttcttattta
1441 gaaaagcaaa tctaaaatta tctgaaaagg gaatgagaat agtgaatgga ccaataataa
1501 tgactagaga agaaagaatg aagattgttc atgaaattaa ggaacgaata ttggataaat
1561 atggggatga tgttaaggct attggtgttt atggctctct tggtcgtcag actgatgggc
1621 cctattcgga tattgagatg atgtgtgtca tgtcaacaga ggaagcagag ttcagccatg
1681 aatggacaac cggtgagtgg aaggtggaag tgaattttga tagcgaagag attctactag
1741 attatgcatc tcaggtggaa tcagattggc cgcttacaca tggtcaattt ttctctattt
1801 tgccgattta tgattcaggt ggatacttag agaaagtgta tcaaactgct aaatcggtag
1861 aagcccaaac gttccacgat gcgatttgtg cccttatcgt agaagagctg tttgaatatg
1921 caggcaaatg gcgtaatatt cgtgtgcaag gaccgacaac atttctacca tccttgactg
1981 tacaggtagc aatggcaggt gccatgttga ttggtctgca tcatcgcatc tgttatacga
2041 cgagcgcttc ggtcttaact gaagcagtta agcaatcaga tcttccttca ggttatgacc
2101 atctgtgcca gttcgtaatg tctggtcaac tttccgactc tgagaaactt ctggaatcgc
2161 tagagaattt ctggaatggg attcaggagt ggacagaacg acacggatat atagtggatg
2221 tgtcaaaacg cataccattt tgaacgatga cctctaataa ttgttaatca tgttggttac
2281 gtatttatta acttctccta gtattagtaa ttatcatggc tgtcatggcg cattaacgga
2341 ataaagggtg tgcttaaatc gggccatttt gcgtaataag aaaaaggatt aattatgagc
2401 gaattgaatt aataataagg taatagattt acattagaaa atgaaagggg attttatgcg
2461 tgagaatgtt acagtctatc ccggcattgc cagtcgggga tattaaaaag agtataggtt
2521 tttattgcga taaactaggt ttcactttgg ttcaccatga agatggattc gcagttctaa
2581 tgtgtaatga ggttcggatt catctatggg aggcaagtga tgaaggctgg cgctctcgta
2641 gtaatgattc accggtttgt acaggtgcgg agtcgtttat tgctggtact gctagttgcc
2701 gcattgaagt agagggaatt gatgaattat atcaacatat taagcctttg ggcattttgc
2761 accccaatac atcattaaaa gatcagtggt gggatgaacg agactttgca gtaattgatc
2821 ccgacaacaa tttgattagc ttttttcaac aaataaaaag ctaaaatcta ttattaatct
2881 gttcagcaat cgggcgcgat tgctgaataa aagatacgag agacctctct tgtatctttt
2941 ttattttgag tggttttgtc cgttacacta gaaaaccgaa agacaataaa aattttattc
3001 ttgctgagtc tggctttcgg taagctagac aaaacggaca aaataaaaat tggcaagggt
3061 ttaaaggtgg agattttttg agtgatcttc tcaaaaaata ctacctgtcc cttgctgatt
3121 tttaaacgag cacgagagca aaacccccct ttgctgaggt ggcagagggc aggttttttt
3181 gtttcttttt tctcgtaaaa aaaagaaagg tcttaaaggt tttatggttt tggtcggcac
3241 tgaattcgag ctcagcatta ttgagtggat gattatattc cttttgatag gtggtatgtt
3301 ttcgcttgaa cttttaaata cagccattga acatacggtt gatttaataa ctgacaaaca
3361 tcaccctctt gctaaagcgg ccaaggacgc tgccgccggg gctgtttgcg tttttgccgt
3421 gatttcgtgt atcattggtt tacttatttt tttgccaaag ctgtaatggc tgaaaattct
3481 tacatttatt ttacattttt agaaatgggc gtgaaaaaaa gcgcgcgatt atgtaaaata
3541 taaagtgata gcggtaccag gagggctgga agaagcagac cgctaacaca gtacataaaa
3601 aaggagacat gaacgatgaa catcaaaaag tttgcaaaac aagcaacagt attaaccttt
3661 actaccgcac tgctggcagg aggcgcaact caagcttttg cctcgagctc ggtacccggg
3721 gatcctctag agtcgacctg caggcatgca agctagcttc agcacaattc caagaaagac
3781 acgattt
//
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