| 质粒名称: | bRA89 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 100950 |
| 存储实验室: | James Haber |
|
载体骨架基本信息 |
|
| 载体名称: | pRS316 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 酵母表达，CRISPR，合成生物学 |
| 筛选标记: | 潮霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | bRA89 was a gift from James Haber (Addgene plasmid # 100950 ; http://n2t.net/addgene:100950 ; RRID:Addgene_100950) |
| 参考文献部分: | Rad51-mediated double-strand break repair and mismatch correction of divergent substrates. Anand R, Beach A, Li K, Haber J. Nature. 2017 Apr 20;544(7650):377-380. doi: 10.1038/nature22046. Epub 2017 Apr 12. 10.1038/nature22046 PubMed 28405019 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS bRA89 11380 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI
sites. HPH marker.
ACCESSION .
VERSION .
KEYWORDS bRA89
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11380)
AUTHORS Anand R, Beach A, Li K, Haber J
TITLE Rad51-mediated double-strand break repair and mismatch correction of
divergent substrates.
JOURNAL Nature. 2017 Apr 20;544(7650):377-380. doi: 10.1038/nature22046.
Epub 2017 Apr 12.
PUBMED 28405019
REFERENCE 2 (bases 1 to 11380)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11380
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(29..51)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 151..170
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
gene complement(284..1858)
/label=hphMX6
/note="yeast selectable marker conferring hygromycin
resistance"
terminator 284..481
/label=TEF terminator
/note="Ashbya gossypii TEF terminator"
CDS complement(487..1515)
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
KE"
promoter complement(1516..1858)
/label=TEF promoter
/note="Ashbya gossypii TEF promoter"
CDS 2876..6979
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
CDS 6992..7012
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 7016..7036
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 7040..7060
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 7061..7126
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 7145..7392
/gene="S. cerevisiae CYC1"
/label=CYC1 terminator
/note="transcription terminator for CYC1"
primer_bind complement(7161..7179)
/label=CYC1
/note="CYC1 transcription termination signal, reverse
primer"
rep_origin complement(7452..7907)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(7589..7610)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 7801..7820
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 8033..8055
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 8047..8064
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 8048..8064
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 8071..8090
/label=T7
/note="T7 promoter, forward primer"
promoter 8071..8089
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 8122..8138
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 8122..8138
/label=pBluescriptSK
/note="For pBluescript vector"
promoter 8170..8438
/label=SNR52 promoter
/note="promoter for the S. cerevisiae small nucleolar RNA
gene SNR52"
misc_RNA 8466..8540
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
terminator 8545..8564
/label=SUP4 terminator
/note="transcription terminator for the S. cerevisiae SUP4
tRNA gene"
promoter complement(8607..8625)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(8646..8662)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8646..8662)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(8659..8681)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 8670..8686
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(8694..8724)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8739..8760
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(8877..8894)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(9048..9636)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(9128..9147)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(9807..10667)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 10430..10449
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(10668..10772)
/gene="bla"
/label=AmpR promoter
misc_feature 10809..11312
/label=CEN/ARS
/note="S. cerevisiae CEN6 centromere fused to an
autonomously replicating sequence"
primer_bind 11353..11371
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accataccac cttttcaatt catcattttt tttttattct tttttttgat ttcgaattcg
241 agctcgttta aactggatgg cggcgttagt atcgaatcga cagcagtata gcgaccagca
301 ttcacatacg attgacgcat gatattactt tctgcgcact taacttcgca tctgggcaga
361 tgatgtcgag gcgaaaaaaa atataaatca cgctaacatt tgattaaaat agaacaacta
421 caatataaaa aaactataca aatgacaagt tcttgaaaac aagaatcttt ttattgtcag
481 tactgattat tcctttgccc tcggacgagt gctggggcgt cggtttccac tatcggcgag
541 tacttctaca cagccatcgg tccagacggc cgcgcttctg cgggcgattt gtgtacgccc
601 gacagtcccg gctccggatc ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc
661 atcgaaattg ccgtcaacca agctctgata gagttggtca agaccaatgc ggagcatata
721 cgcccggagc cgcggcgatc ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg
781 ctgctccata caagccaacc acggcctcca gaagaagatg ttggcgacct cgtattggga
841 atccccgaac atcgcctcgc tccagtcaat gaccgctgtt atgcggccat tgtccgtcag
901 gacattgttg gagccgaaat ccgcgtgcac gaggtgccgg acttcggggc agtcctcggc
961 ccaaagcatc agctcatcga gagcctgcgc gacggacgca ctgacggtgt cgtccatcac
1021 agtttgccag tgatacacat ggggatcagc aatcgcgcat atgaaatcac gccatgtagt
1081 gtattgaccg attccttgcg gtccgaatgg gccgaacccg ctcgtctggc taagatcggc
1141 cgcagcgatc gcatccatgg cctccgcgac cggctgcaga acagcgggca gttcggtttc
1201 aggcaggtct tgcaacgtga caccctgtgc acggcgggag atgcaatagg tcaggctctc
1261 gctgaattcc ccaatgtcaa gcacttccgg aatcgggagc gcggccgatg caaagtgccg
1321 ataaacataa cgatctttgt agaaaccatc ggcgcagcta tttacccgca ggacatatcc
1381 acgccctcct acatcgaagc tgaaagcacg agattcttcg ccctccgaga gctgcatcag
1441 gtcggagacg ctgtcgaact tttcgatcag aaacttctcg acagacgtcg cggtgagttc
1501 aggcttttta cccatggttg tttatgttcg gatgtgatgt gagaactgta tcctagcaag
1561 attttaaaag gaagtatatg aaagaagaac ctcagtggca aatcctaacc ttttatattt
1621 ctctacaggg gcgcggcgtg gggacaattc aacgcgtctg tgaggggagc gtttccctgc
1681 tcgcaggtcc gcagcgagga gccgtaattt ttgcttcgcg ccgtgcggcc atcaaaatgt
1741 atggatgcaa atgattatac atggggatgt atgggctaaa tgtacgggcg acagtcacat
1801 catgcccctg agctgcgcac gtcaagactg tcaaggaggt attctgggcc tccatgtcgc
1861 tggccgggtg acccggcggg gaaagtacct tcaaagaatg gggtcttatc ttgttttgca
1921 agtaccactg agcaggataa taatagaaat gataatatac tatagtagag ataacgtcga
1981 tgacttccca tactgtaatt gcttttagtt gtgtattttt agtgtgcaag tttctgtaaa
2041 tcgattaatt tttttttctt tcctcttttt attaacctta atttttattt tagattcctg
2101 acttcaactc aagacgcaca gatattataa catctgcata ataggcattt gcaagaatta
2161 ctcgtgagta aggaaagagt gaggaactat cgcatacctg catttaaaga tgccgatttg
2221 ggcgcgaatc ctttattttg gcttcaccct catactatta tcagggccag aaaaaggaag
2281 tgtttccctc cttcttgaat tgatgttacc ctcataaagc acgtggcctc ttatcgagaa
2341 agaaattacc gtcgctcgtg atttgtttgc aaaaagaaca aaactgaaaa aacccagaca
2401 cgctcgactt cctgtcttcc tattgattgc agcttccaat ttcgtcacac aacaaggtcc
2461 tagcgacggc tcacaggttt tgtaacaagc aatcgaaggt tctggaatgg cgggaaaggg
2521 tttagtacca catgctatga tgcccactgt gatctccaga gcaaagttcg ttcgatcgta
2581 ctgttactct ctctctttca aacagaattg tccgaatcgt gtgacaacaa cagcctgttc
2641 tcacacactc ttttcttcta accaaggggg tggtttagtt tagtagaacc tcgtgaaact
2701 tacatttaca tatatataaa cttgcataaa ttggtcaatg caagaaatac atatttggtc
2761 ttttctaatt cgtagttttt caagttctta gatgctttct ttttctcttt tttacagatc
2821 atcaaggaag taattatcta ctttttacaa caaatataaa acaccggatc tcaaaatgga
2881 taaaaagtat agtattggtt tagatattgg tactaactct gtgggttggg cagttatcac
2941 tgacgaatat aaagttccat caaagaaatt taaggtgtta ggtaacactg acagacactc
3001 aataaaaaag aatcttatcg gtgctctttt gttcgactcc ggtgaaactg ccgaggctac
3061 acgtttaaaa agaacagcaa gaagaagata tacccgtaga aaaaatagaa tatgttattt
3121 acaagaaatc ttttctaatg aaatggctaa agttgatgat tcctttttcc atagattgga
3181 agagtcattt ttggttgaag aagacaaaaa gcatgagaga catccaatct ttgggaatat
3241 agttgatgaa gtggcttacc atgaaaaata tcctaccatt tatcatttaa gaaagaaatt
3301 ggtagattca actgataaag ctgaccttag attaatctat ttagcacttg cccatatgat
3361 taaatttaga ggtcattttt tgattgaagg tgatttgaac ccagataatt ctgacgtgga
3421 taaattattt attcaattag tccaaaccta caaccaatta tttgaggaaa atccaattaa
3481 tgctagtggt gtcgatgcca aagctatatt atcagccaga ttatcaaaat ctagacgttt
3541 ggaaaatttg attgcccaat tgccaggaga aaaaaagaat ggattatttg gaaacttgat
3601 cgcattatca ttgggtttga caccaaattt taaatctaat tttgatttag ctgaagatgc
3661 taaattacaa ttatcaaaag acacctatga cgacgatttg gacaatttac ttgctcaaat
3721 tggtgatcaa tatgcagatt tgttcttagc tgctaaaaac ttatctgatg ctattttgtt
3781 gtctgatatt ttgagagtga acacagaaat aaccaaagct ccattatcag catctatgat
3841 caaacgttat gatgaacacc atcaggattt gactttattg aaagctttgg tgagacaaca
3901 attgccagag aagtataaag aaatcttttt cgatcaatct aaaaacgggt atgcaggtta
3961 tattgatggg ggtgcctccc aagaggaatt ttacaaattt ataaaaccta ttttagaaaa
4021 gatggatggg actgaggaac ttttggtcaa attgaacaga gaagatttgt tacgtaaaca
4081 gagaactttt gataatggta gtatacctca ccaaattcat ttgggtgagt tgcatgcaat
4141 tttaagaaga caagaagatt tttatccatt tttaaaagat aatagagaaa aaatcgagaa
4201 aattttaacc tttagaattc catactatgt tgggcctttg gctagaggta attcaagatt
4261 tgcctggatg acacgtaaat cagaagaaac tattacccct tggaattttg aagaggttgt
4321 tgataaagga gcatcagcac agagttttat tgaaagaatg accaatttcg ataaaaactt
4381 accaaatgaa aaagttttac caaaacattc cttgttatac gaatatttta ctgtttacaa
4441 tgaacttaca aaggttaaat atgttactga aggtatgcgt aagccagcct ttttatctgg
4501 agaacagaaa aaggcaatag ttgatttatt gtttaaaaca aatagaaaag ttactgttaa
4561 acaattaaaa gaagattact ttaagaaaat tgaatgtttt gattcagttg aaatcagtgg
4621 tgttgaagac agatttaatg ctagtttagg aacttaccat gatttactta aaattatcaa
4681 agataaagat ttcttggata acgaagaaaa tgaagacatt ttagaagaca ttgttttaac
4741 cttaacttta ttcgaagata gagagatgat tgaagaacgt ttgaagactt atgcacattt
4801 gtttgacgat aaagtgatga aacagttgaa aagaagacgt tatactggat ggggtagatt
4861 gtctcgtaaa ttgatcaatg gaattagaga taaacaaagt ggtaaaacta tcttggactt
4921 tttgaaatct gacggatttg ctaatagaaa tttcatgcaa ttgatccacg acgatagttt
4981 gacatttaaa gaagacatcc aaaaggccca agtgagtggg caaggtgatt cattacatga
5041 acatattgca aatttagccg gatctcctgc tattaagaaa gggatattac aaactgttaa
5101 agttgtggat gaattagtga aagtaatggg aagacataaa cctgaaaaca ttgtcattga
5161 gatggcaaga gaaaatcaaa ctacacaaaa aggacagaaa aatagtagag aacgtatgaa
5221 aagaatagaa gagggtatta aagaattggg tagtcaaata ttgaaagaac acccagtgga
5281 aaatacccag ttgcaaaatg aaaaattata tctttactac cttcaaaatg gacgtgatat
5341 gtatgttgat caggaattag atataaatag actttcagat tatgatgtag atcatatagt
5401 tccacaatct ttcttgaaag atgattccat agacaataaa gtattaacta gaagtgataa
5461 aaatagaggt aaaagtgata atgtcccaag tgaggaagtc gtcaaaaaga tgaaaaatta
5521 ctggcgtcaa cttttgaatg ctaaattaat tactcaaaga aaatttgata atttgactaa
5581 agcagaaaga ggtgggcttt ctgaattaga taaagccggg ttcattaaaa gacaattggt
5641 cgaaactaga caaattacta aacatgttgc ccaaatttta gattcccgta tgaacactaa
5701 gtatgacgaa aatgataagt taatacgtga ggttaaagtc attactttaa aatcaaaact
5761 tgtctctgat ttcagaaagg atttccaatt ctataaagtt agagaaatta ataattatca
5821 tcatgctcat gatgcatatt tgaatgctgt agttggaact gctttaatca agaaataccc
5881 taaattagaa tctgaatttg tatatggtga ttacaaagtc tatgatgtta gaaagatgat
5941 tgctaaatca gaacaagaaa ttggtaaagc tacagctaaa tacttctttt actctaacat
6001 tatgaatttc tttaaaacag aaattacttt ggcaaacggt gaaattagaa aaagacctct
6061 tattgaaaca aatggtgaga ctggagagat agtttgggac aaagggcgtg atttcgctac
6121 tgttagaaaa gttttatcaa tgccacaagt taacattgta aagaaaacag aggttcaaac
6181 tggtggtttc tcaaaagaaa gtattttgcc taaaagaaat agtgataaat tgattgccag
6241 aaaaaaggat tgggatccaa agaaatatgg tggtttcgac tcaccaaccg tagcctattc
6301 tgttttggtt gtggcaaagg ttgaaaaggg taaaagtaaa aagcttaaat cagtaaaaga
6361 acttttgggt attacaataa tggaaagaag ttcctttgaa aagaacccta ttgatttttt
6421 ggaagctaaa ggttataagg aagtaaagaa ggacttaata atcaaattgc ctaaatattc
6481 tttatttgaa ttagaaaatg ggagaaaaag aatgttggct tctgctggag aattgcaaaa
6541 gggtaatgaa ttagcattgc cttccaaata tgttaacttc ttgtatttag cttcacacta
6601 tgaaaagttg aaagggtcac cagaagataa cgagcaaaaa caattatttg ttgaacaaca
6661 caaacactac ttagatgaga ttatagaaca aattagtgaa ttcagtaaaa gagtgatatt
6721 agctgatgca aatttagata aagttttgtc agcctataac aaacatagag ataagccaat
6781 tagagaacaa gcagaaaaca ttattcactt atttaccctt accaatttag gagcacctgc
6841 tgctttcaag tattttgata caacaattga tcgtaaaaga tatacctcaa caaaagaagt
6901 cttagacgcc accttaattc atcaatcaat cactggattg tatgagacaa gaattgattt
6961 gtctcaattg ggtggtgatg aaggggctga tcctaagaag aaaagaaaag ttgatccaaa
7021 gaaaaagcgt aaggtggatc ctaagaaaaa gagaaaggtt gactacaaag accatgacgg
7081 tgattataaa gatcatgaca tcgactacaa ggatgacgat gacaagtgat aatctcttct
7141 cgagtcatgt aattagttat gtcacgctta cattcacgcc ctccccccac atccgctcta
7201 accgaaaagg aaggagttag acaacctgaa gtctaggtcc ctatttattt ttttatagtt
7261 atgttagtat taagaacgtt atttatattt caaatttttc ttttttttct gtacagacgc
7321 gtgtacgcat gtaacattat actgaaaacc ttgcttgaga aggttttggg acgctcgaag
7381 gctttaattt gcggccggta ctgcggtgtg aaataccgca cagatgcgta aggagaaaat
7441 accgcatcag gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
7501 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
7561 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
7621 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
7681 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
7741 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
7801 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
7861 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcca ttcgccattc
7921 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
7981 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
8041 cgacgttgta aaacgacggc cagtgaattg taatacgact cactataggg cgaattggag
8101 ctccaccgcg gtggcggccg ctctagaact agtggatccc ccgggctgca ggaattcgat
8161 atcaagcttt ctttgaaaag ataatgtatg attatgcttt cactcatatt tatacagaaa
8221 cttgatgttt tctttcgagt atatacaagg tgattacatg tacgtttgaa gtacaactct
8281 agattttgta gtgccctctt gggctagcgg taaaggtgcg cattttttca caccctacaa
8341 tgttctgttc aaaagatttt ggtcaaacgc tgtagaagtg aaagttggtg cgcatgtttc
8401 ggcgttcgaa acttctccgc agtgaaagat aaatgatcct agatgcgaga tatcctcatg
8461 cctaggtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa
8521 aaagtggcac cgagtcggtg gtgctttttt tgttttttat gtctaccgtc gacctcgagg
8581 gggggcccgg tacccagctt ttgttccctt tagtgagggt taatttcgag cttggcgtaa
8641 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
8701 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
8761 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
8821 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
8881 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
8941 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
9001 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
9061 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
9121 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
9181 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
9241 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
9301 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
9361 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
9421 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
9481 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
9541 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
9601 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
9661 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
9721 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
9781 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
9841 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
9901 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
9961 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
10021 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
10081 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
10141 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
10201 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
10261 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
10321 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
10381 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
10441 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
10501 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
10561 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
10621 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
10681 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
10741 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctggg
10801 tccttttcat cacgtgctat aaaaataatt ataatttaaa ttttttaata taaatatata
10861 aattaaaaat agaaagtaaa aaaagaaatt aaagaaaaaa tagtttttgt tttccgaaga
10921 tgtaaaagac tctaggggga tcgccaacaa atactacctt ttatcttgct cttcctgctc
10981 tcaggtatta atgccgaatt gtttcatctt gtctgtgtag aagaccacac acgaaaatcc
11041 tgtgatttta cattttactt atcgttaatc gaatgtatat ctatttaatc tgcttttctt
11101 gtctaataaa tatatatgta aagtacgctt tttgttgaaa ttttttaaac ctttgtttat
11161 ttttttttct tcattccgta actcttctac cttctttatt tactttctaa aatccaaata
11221 caaaacataa aaataaataa acacagagta aattcccaaa ttattccatc attaaaagat
11281 acgaggcgcg tgtaagttac aggcaagcga tccgtctaag aaaccattat tatcatgaca
11341 ttaacctata aaaataggcg tatcacgagg ccctttcgtc
//
载体图谱质粒图谱pdf版和相关资料下载
载体质粒应用举例
- 上一篇：pBK-YEE-BE3
- 下一篇：Fuw-dCas9-Dnmt3a_IM
