pAAVLINKv2-SCP1-Cre-UDeg3a-3'-GLI3-FLAG

LOCUS       pAAVLINKv2-SCP1-Cre-UDeg3a-3'-GLI3-FLAG                6254 bp DNA     circular SYN 18-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6254)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported May 20, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6254
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     misc_feature    145..225
                     /label=SCP1
     CDS             237..1265
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1281..1370
                     /codon_start=1
                     /label=UDeg3a
                     /translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
     misc_feature    1295..1300
                     /label=SDS
     misc_feature    1298..1303
                     /label=SDS
     intron          1374..1414
                     /label=Splicing Donor
     protein_bind    1421..1454
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1455..1546
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1547..2967
                     /codon_start=1
                     /label=GLI3
                     /translation="GTSSTSPAPSRTTAKCPTGPVTLTRPGCQTATLASSSMPSSSPAP
                     RAAKPTCPFSGTKSAPEAPTCPPPSSSVGRGPLCRRLAPLGSATAWSSTRRTP*GAGLL
                     GAIRPSGRTATPTVAQST*CSTTAPEVAPVETPSMNSPVRPRSMGTVSTGSQWPLVHST
                     VPVVPGFKPQS*RAPPCKGAGAS*ISACR*RQMSQLAAW*MACRTRTQWDRGTWLTSSS
                     ATACSTRGQAAPVSRCLGRLVLPHTSTSTKGQRAACQGLTAWAASRQAWQLSGATSHVP
                     ALGAAGARLCRGTALLCSQDSSVTQVRPAG*MVSRWR*KGSPIRCALICRITLVSSMTK
                     PWASVSKTRKLVHSLFQTPAACYRGPAPKTLSYFPQVLIR*QAQWTASTAMTWKGYRLT
                     SMPS*TMGTTPA*CRGP*AQVSFRTFPIAPPASPRLGRPSHSQRCP*APPTWLSGT*VL
                     C*PP*RKKANSLQLC"
     misc_feature    1745..1750
                     /label=SDS
     CDS             2974..3039
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3043..3474
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    3187..3192
                     /label=SDS
     repeat_region   3517..3657
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    3517..3646
                     /label=AAV2 ITR
     rep_origin      3732..4187
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4469..4573
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4574..5434
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5605..6193
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
      181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
      241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
      301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
      361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
      421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
      481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
      541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
      601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
      661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
      721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
      781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
      841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
      901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
      961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
     1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
     1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
     1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
     1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     1501 gcacctattg gtcttactga catccacttt gcctttctct ccacagggta cgagcagcac
     1561 ttccccagcg ccctcccgga cgacagcaaa gtgccccacg ggcccggtga ctttgacgcg
     1621 cccgggctgc cagacagcca cgctggccag cagttccatg ccctcgagca gccctgcccc
     1681 gagggcagca aaaccgacct gcccattcag tggaacgaag tcagctccgg aagcgccgac
     1741 ctgtcctcct ccaagctcaa gtgtgggccg cggcccgctg tgccgcagac tcgcgccttt
     1801 gggttctgca acggcatggt cgtccacccg cagaacccct tgaggagcgg gcctgctggg
     1861 ggctatcaga ccctcgggga gaacagcaac ccctacggtg gcccagagca cttgatgctc
     1921 cacaacagcc ccggaagtgg caccagtgga aacgccttcc atgaacagcc ctgtaaggcc
     1981 ccgcagtatg ggaactgtct caacaggcag ccagtggccc ctggtgcact cgacggtgcc
     2041 tgtggtgccg ggattcaagc ctcaaagctg aagagcaccc ccatgcaagg gagcgggggc
     2101 cagctgaatt tcggcctgcc ggtagcgcca aatgagtcag ctggcagcat ggtgaatggc
     2161 atgcagaacc aggacccagt gggacagggg tacctggctc accagctcct cggcgacagc
     2221 atgcagcacc cgggggcagg ccgccccggt cagcagatgc ttgggcagat tagtgctacc
     2281 tcacacatca acatctacca agggccagag agctgcctgc caggggctca cggcatgggc
     2341 agccagccgt caagcttggc agttgtcagg ggctaccagc catgtgccag ctttgggggc
     2401 agcaggcgcc aggctatgcc gagggacagc cttgctctgc agtcaggaca gctcagtgac
     2461 acaagtcaga cctgcagggt gaatggtatc aagatggaga tgaaagggca gccccatccg
     2521 ctgtgctcta atctgcagaa ttactctggt cagttctatg accaaaccgt gggcttcagt
     2581 cagcaagaca cgaaagctgg ttcattctct atttcagacg ccagctgcct gctacagggg
     2641 accagcgcca aaaactctga gttactttcc ccaggtgcta atcaggtgac aagcacagtg
     2701 gacagcctcg acagccatga cctggaaggg gtacagattg acttcgatgc catcatagac
     2761 gatggggacc actccagcct gatgtcgggg gccctgagcc caagtatcat tcagaacctt
     2821 tcccatagct cctcccgcct caccacgcct cgggcgtccc tcccattccc agcgctgtcc
     2881 atgagcacca ccaacatggc tatcggggac atgagttctt tgctgacctc cctagcggaa
     2941 gaaagcaaat tccttgcagt tatgcaatgt acagactaca aagaccatga cggtgattat
     3001 aaagatcatg acatcgacta caaggatgac gatgacaagt aactcgagat aatcaacctc
     3061 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     3121 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     3181 ttttctcctc cttgtataaa tcctggttag ttcttgccac ggcggaactc atcgccgcct
     3241 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttta
     3301 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat ctagctttat ttgtgaaatt
     3361 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     3421 aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt ttaaagatct
     3481 ggagaccggt gtggcacgtg cggaccgagc ggccgcagga acccctagtg atggagttgg
     3541 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac
     3601 gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc
     3661 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa
     3721 gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     3781 cagcgtgacc gctacacttg ccagcgcctt agcgcccgct cctttcgctt tcttcccttc
     3841 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     3901 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc
     3961 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     4021 ctttaatagt ggactcttgt tccaaactgg aacaacactc aactctatct cgggctattc
     4081 ttttgattta taagggattt tgccgatttc ggtctattgg ttaaaaaatg agctgattta
     4141 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct
     4201 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
     4261 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     4321 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
     4381 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     4441 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     4501 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     4561 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     4621 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     4681 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     4741 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     4801 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     4861 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     4921 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     4981 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     5041 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     5101 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     5161 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     5221 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     5281 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     5341 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     5401 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     5461 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     5521 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     5581 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     5641 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     5701 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     5761 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     5821 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     5881 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     5941 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     6001 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     6061 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     6121 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     6181 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     6241 ttttgctcac atgt
//