| 质粒名称: | pTU2-AmyE-Pveg-eGFP |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 112792 |
| 存储实验室: | Chris French |
|
载体骨架基本信息 |
|
| 载体名称: | pSB1C3 |
| 载体出品公司: | iGEM |
| 载体抗性: | 氯霉素 |
| 空载体大小: | 3185 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 细菌表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Pveg-eGFP |
| 物种来源: | Synthetic |
| 插入大小: | 1400 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | BsmB1（销毁后未知） |
| 3' 克隆位点: | BsmB1（销毁后未知） |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pTU2-AmyE-Pveg-eGFP was a gift from Chris French (Addgene plasmid # 112792 ; http://n2t.net/addgene:112792 ; RRID:Addgene_112792) |
| 参考文献部分: | BacilloFlex: A modular DNA assembly toolkit for Bacillus subtilis synthetic biology. Wicke N, Radford D, Verrone V, Wipat A, French CE. bioRxiv 185108 10.1101/185108 |
买家导航
pTU2-AmyE-Pveg-eGFP载体质粒基本信息
pTU2-AmyE-Pveg-eGFP质粒图谱载体图谱和pTU2-AmyE-Pveg-eGFP载体序列质粒序列多克隆位点信息
pTU2-AmyE-Pveg-eGFP质粒载体简介
pTU2-AmyE-Pveg-eGFP质粒序列载体序列
LOCUS pTU2-AmyE-Pveg-eGFP 4978 bp ds-DNA circular SYN 14-AUG-2018
DEFINITION Expresses GFP under the control of Pveg promoter and it is
integrated into AmyE locus. Used to test correct transformation.
ACCESSION .
VERSION .
KEYWORDS pTU2-AmyE-Pveg-eGFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4978)
AUTHORS Wicke N, Radford D, Verrone V, Wipat A, French CE
TITLE BacilloFlex: A modular DNA assembly toolkit for Bacillus subtilis
synthetic biology
JOURNAL bioRxiv
REFERENCE 2 (bases 1 to 4978)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 14, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4978
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 1..22
/label=BioBrick prefix
/note="BioBrick prefix for parts that do not start with
""ATG"""
CDS 678..1394
/codon_start=1
/product="Aequoria victoria green fluorescent protein"
/label=GFP
/translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITHGMDELYK"
primer_bind complement(708..736)
/label=GFP-R
/note="GFP, reverse primer. Does NOT anneal to EGFP"
primer_bind 1331..1351
/label=GFP-F
/note="GFP, forward primer. Does NOT anneal to EGFP"
terminator 1407..1478
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 1494..1521
/label=T7Te terminator
/note="phage T7 early transcription terminator"
terminator 2952..3009
/label=his operon terminator
/note="This putative transcriptin terminator from the E.
coli his operon has a 2-bp deletion introduced during
synthesis. Its efficiency has not been determined."
primer_bind complement(3033..3050)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(3204..3792)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3284..3303)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
terminator 3975..4069
/label=lambda t0 terminator
/note="transcription terminator from phage lambda"
CDS complement(4090..4749)
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
primer_bind 4664..4683
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
primer_bind 4911..4929
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
terminator 4933..4976
/label=bacterial terminator
/note="putative bacterial transcription terminator"
ORIGIN
1 gaattcgcgg ccgcttctag aggtctcact atatcttttg caaaacgatt caaaacctct
61 ttactgccgt tattcgctgg atttttattg ctgtttcatt tggttctggc aggaccggcg
121 gctgcgagtg ctgaaacggc gaacaaatcg aatgagctta cagcaccgtc gatcaaaagc
181 ggaaccattc ttcatgcatg gaattggtcg ttcaatacgt taaaacacaa tatgaaggat
241 attcatgatg caggatatac agccattcag acatctccga ttaaccaagt aaaggaaggg
301 aatcaaggag ataaaagcat gtcgaactgg tactggctgt atcagccgac atcgtatcaa
361 attggcaacc gttacttagg tactgaacaa gaatttaaag aaatgtgtgc agccgctgaa
421 gaatatggca taaaggtcat tgttgacgcg gtcatcaatc ataccaccag tgattatgcc
481 gcgatttcca atgaggttaa gagtattcca aactggacac atggaaacac acaaattaaa
541 aactggtctt gccctataat tttgtcaaaa taattttatt gacaacgtct tattaacgtt
601 gatataattt aaattttatt tgacaaaaat gggctcgtgt tgtacaataa atgtgtacat
661 attaagagga ggagcatatg cgtaaaggag aagaactttt cactggagtt gtcccaattc
721 ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag
781 gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg
841 ttccatggcc aacacttgtc actactttcg gttatggtgt tcaatgcttt gcgagatacc
901 cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg
961 aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg
1021 aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa
1081 acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag
1141 acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa
1201 gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt
1261 taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga
1321 gagatcacat ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg
1381 aactatacaa ataatcgacc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
1441 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
1501 cttcgggtgg gcctttctgc gtttatatgt tccggcaccc attagttcaa caaacgaaaa
1561 ttggataaag tgggatattt ttaaaatata tatttatgtt acaataatat tgacttttaa
1621 aaaaggattg attctaatga agaaagcaga caagtaagcc tcctaaattc actttagata
1681 aaaatttagg aggcatatca aatgaacttt aataaaattg atttagacaa ttggaagaga
1741 aaagagatat ttaatcatta tttgaaccaa caaacgactt ttagtataac cacagaaatt
1801 gatattagtg ttttataccg aaacataaaa caagaaggat ataaatttta ccctgcattt
1861 attttcttag tgacaagggt gataaactca aatacagctt ttagaactgg ttacaatagc
1921 gacggagagt taggttattg ggataagtta gagccacttt atacaatttt tgatggtgta
1981 tctaaaacat tctctggtat ttggactcct gtaaagaatg acttcaaaga gttttatgat
2041 ttataccttt ctgatgtaga gaaatataat ggttcgggga aattgtttcc caaaacacct
2101 atacctgaaa atgctttttc tctttctatt attccatgga cttcatttac tgggtttaac
2161 ttaaatatca ataataatag taattacctt ctacccatta ttacagcagg aaaattcatt
2221 aataaaggta attcaatata tttaccgcta tctttacagg tacatcattc tgtttgtgat
2281 ggttatcatg caggattgtt tatgaactct attcaggaat tgtcagatag gcctaatgac
2341 tggcttttat aatatgagat aatgccgact gtacttttta cagtcggaag ttaggctggg
2401 cggtgatagc ttctcgttca ggcagtacgc ctcttttctt ttccagacct gagggaggcg
2461 gaaatggtgt gaggttcccg gggaaaagcc aaataggcga tcgcgggagt gctttatttg
2521 aagatcaggc tatcactgcg gtcaatagat ttcacaatgt gatggctgga cagcctgagg
2581 aactctcgaa cccgaatgga aacaaccaga tatttatgaa tcagcgcggc tcacatggcg
2641 ttgtgctggc aaatgcaggt tcatcctctg tctctatcaa tacggcaaca aaattgcctg
2701 atggcaggta tgacaataaa gctggagcgg gttcatttca agtgaacgat ggtaaactga
2761 caggcacgat caatgccagg tctgtagctg tgctttatcc tgatgatatt gcaaaagcgc
2821 ctcatgtttt ccttgagaat tacaaaacag gtgtaacaca ttctttcaat gatcaactga
2881 cgattacctt gcgtgcagat gcgaatacaa caaaagccgt ttatcaaatc ttaggtacag
2941 agaccctgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa
3001 ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct gcgctcggtc
3061 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
3121 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
3181 aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga gcatcacaaa
3241 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
3301 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
3361 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
3421 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
3481 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
3541 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
3601 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
3661 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
3721 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
3781 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
3841 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
3901 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
3961 agctcgaggc ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac
4021 aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc
4081 gatatcaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat
4141 tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag
4201 caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc
4261 catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg ctgacacgaa
4321 aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac
4381 atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga
4441 tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat
4501 caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc
4561 aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa
4621 ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc
4681 ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt
4741 tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg
4801 tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaactcgag
4861 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
4921 tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctg
//
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