pCas3cRh

LOCUS       pCas3cRh               11500 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expresses all necessary components of Type I-C CRISPR-Cas system.
ACCESSION   .
VERSION     .
KEYWORDS    pCas3cRh
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11500)
  AUTHORS   Csorgo B, Leon LM, Chau-Ly IJ, Vasquez-Rifo A, Berry JD, Mahendra C,
            Crawford ED, Lewis JD, Bondy-Denomy J
  TITLE     A compact Cascade-Cas3 system for targeted genome engineering.
  JOURNAL   Nat Methods. 2020 Oct 19. pii: 10.1038/s41592-020-00980-w. doi: 
            10.1038/s41592-020-00980-w.
  PUBMED    33077967
REFERENCE   2  (bases 1 to 11500)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11500
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(590..618)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      659..1010
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from 
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     CDS             1024..1857
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pRO1600 from Pseudomonas aeruginosa "
                     /label=pRO1600 Rep
                     /translation="MASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV
                     MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT
                     IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG
                     QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL
                     LFSFGPKKPAKAVGKAPARRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA"
     CDS             complement(1858..2706)
                     /codon_start=1
                     /gene="E. coli rhaR"
                     /product="transcriptional activator of rhaR and rhaS"
                     /label=rhaR
                     /note="needed for rhamnose-dependent activation of the rhaB
                     promoter"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVW
                     RGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAI
                     PGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHR
                     YTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTIN
                     QYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQK
                     D"
     CDS             complement(2780..3616)
                     /codon_start=1
                     /gene="E. coli rhaS"
                     /product="positive regulator of the rhaB promoter
                     "
                     /label=rhaS
                     /note="activated by L-rhamnose"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGT
                     GIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQ
                     ELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQ
                     ENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNR
                     LRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ"
     promoter        3762..3880
                     /label=rhaB promoter
                     /note="promoter of the E. coli rhaBAD operon, conferring 
                     tight induction with L-rhamnose and repression with 
                     D-glucose in the presence of RhaR and RhaS (Giacalone et 
                     al., 2006)"
     regulatory      5813..5822
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             complement(6989..7000)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     oriT            9801..9909
                     /note="incP origin of transfer"
     rep_origin      complement(9979..10567)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10059..10078)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11368..401)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 gcctcatgct tgaggagatt gatgagcgcg gtggcaatgc cctgcctccg gtgctcgccg
       61 gagactgcga gatcatagat atagatctca ctacgcggct gctcaaactt gggcagaacg
      121 taagccgcga gagcgccaac aaccgcttct tggtcgaagg cagcaagcgc gatgaatgtc
      181 ttactacgga gcaagttccc gaggtaatcg gagtccggct gatgttggga gtaggtggct
      241 acgtctccga actcacgacc gaaaagatca agagcagccc gcatggattt gacttggtca
      301 gggccgagcc tacatgtgcg aatgatgccc atacttgagc cacctaactt tgttttaggg
      361 cgactgccct gctgcgtaac atcgttgctg ctgcgtaaca tcgttgctgc tccataacat
      421 caaacatcga cccacggcgt aacgcgcttg ctgcttggat gcccgaggca tagactgtac
      481 aaaaaaacag tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
      541 ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac ttgcattaca gtttacgaac
      601 cgaacaggct tatgtcaatt cgcgccgctg gtgccgctgg ttggacgcca agggtgaatc
      661 cgcctcgata ccctgattac tcgcttcctg cgccctctca ggcggcgata ggggactggt
      721 aaaacgggga ttgcccagac gcctcccccg ccccttcagg ggcacaaatg cggccccaac
      781 ggggccacgt agtggtgcgt tttttgcgtt tccacccttt tcttcctttt cccttttaaa
      841 ccttttagga cgtctacagg ccacgtaatc cgtggcctgt agagtttaaa aagggacgga
      901 tttgttgcca ttaagggacg gatttgttgt taagaaggga cggatttgtt gttgtaaagg
      961 gacggatttg ttgtattgtg ggacgcagat acagtgtccc cttatacaca aggaatgtcg
     1021 aacgtggcct cacccccaat ggtttacaaa agcaatgccc tggtcgaggc cgcgtatcgc
     1081 ctcagtgttc aggaacagcg gatcgttctg gcctgtatta gccaggtgaa gaggagcgag
     1141 cctgtcaccg atgaagtgat gtattcagtg acggcggagg acatagcgac gatggcgggt
     1201 gtccctatcg aatcttccta caaccagctc aaagaagcgg ccctgcgcct gaaacggcgg
     1261 gaagtccggt taacccaaga gcccaatggc aaggggaaaa gaccgagtgt gatgattacc
     1321 ggctgggtgc aaacaatcat ctaccgggag ggtgagggcc gtgtagaact caggttcacc
     1381 aaagacatgc tgccgtacct gacggaactc accaaacagt tcaccaaata cgccttggct
     1441 gacgtggcca agatggacag cacccacgcg atcaggcttt acgagctgct catgcaatgg
     1501 gacagcatcg gccagcgcga aatagaaatt gaccagctgc gaaagtggtt tcaactggaa
     1561 ggccggtatc cctcgatcaa ggacttcaag ttgcgagtgc ttgatccagc cgtgacgcag
     1621 atcaacgagc acagcccgct acaggtggag tgggcgcagc gaaagaccgg gcgcaaggtc
     1681 acacatctgt tgttcagttt tggaccgaag aagcccgcca aggcggtggg taaggcccca
     1741 gcgaggcgca aggccgggaa gatttcagat gctgagatcg cgaaacaggc tcgccctggt
     1801 gagacatggg aagcggcccg cgctcgacta acccagatgc cgctggatct ggcctagtta
     1861 atctttctgc gaattgagat gacgccactg gctgggcgtc atcccggttt cccgggtaaa
     1921 caccaccgaa aaatagttac tatcttcaaa gccacattcg gtcgaaatat cactgattaa
     1981 caggcggcta tgctggagaa gatattgcgc atgacacact ctgacctgtc gcagatattg
     2041 attgatggtc attccagtct gctggcgaaa ttgctgacgc aaaacgcgct cactgcacga
     2101 tgcctcatca caaaatttat ccagcgcaaa gggacttttc aggctagccg ccagccgggt
     2161 aatcagctta tccagcaacg tttcgctgga tgttggcggc aacgaatcac tggtgtaacg
     2221 atggcgattc agcaacatca ccaactgccc gaacagcaac tcagccattt cgttagcaaa
     2281 cggcacatgc tgactacttt catgctcaag ctgaccgata acctgccgcg cctgcgccat
     2341 ccccatgcta cctaagcgcc agtgtggttg ccctgcgctg gcgttaaatc ccggaatcgc
     2401 cccctgccag tcaagattca gcttcagacg ctccgggcaa taaataatat tctgcaaaac
     2461 cagatcgtta acggaagcgt aggagtgttt atcgtcagca tgaatgtaaa agagatcgcc
     2521 acgggtaatg cgataagggc gatcgttgag tacatgcagg ccattaccgc gccagacaat
     2581 caccagctca caaaaatcat gtgtatgttc agcaaagaca tcttgcggat aacggtcagc
     2641 cacagcgact gcctgctggt cgctggcaaa aaaatcatct ttgagaagtt ttaactgatg
     2701 cgccaccgtg gctacctcgg ccagagaacg aagttgatta ttcgcaatat ggcgtacaaa
     2761 tacgttgaga agattcgcgt tattgcagaa agccatcccg tccctggcga atatcacgcg
     2821 gtgaccagtt aaactctcgg cgaaaaagcg tcgaaaagtg gttactgtcg ctgaatccac
     2881 agcgataggc gatgtcagta acgctggcct cgctgtggcg tagcagatgt cgggctttca
     2941 tcagtcgcag gcggttcagg tatcgctgag gcgtcagtcc cgtttgctgc ttaagctgcc
     3001 gatgtagcgt acgcagtgaa agagaaaatt gatccgccac ggcatcccaa ttcacctcat
     3061 cggcaaaatg gtcctccagc caggccagaa gcaagttgag acgtgatgcg ctgttttcca
     3121 ggttctcctg caaactgctt ttacgcagca agagcagtaa ttgcataaac aagatctcgc
     3181 gactggcggt cgagggtaaa tcattttccc cttcctgctg ttccatctgt gcaaccagct
     3241 gtcgcacctg ctgcaatacg ctgtggttaa cgcgccagtg agacggatac tgcccatcca
     3301 gctcttgtgg cagcaactga ttcagcccgg cgagaaactg aaatcgatcc ggcgagcgat
     3361 acagcacatt ggtcagacac agattatcgg tatgttcata cagatgccga tcatgatcgc
     3421 gtacgaaaca gaccgtgcca ccggtgatgg tatagggctg cccattaaac acatgaatac
     3481 ccgtgccatg ttcgacaatc acaatttcat gaaaatcatg atgatgttca ggaaaatccg
     3541 cctgcgggag ccggggttct atcgccacgg acgcgttacc agacggaaaa aaatccacac
     3601 tatgtaatac ggtcatactg gcctcctgat gtcgtcaaca cggcgaaata gtaatcacga
     3661 ggtcaggttc ttaccttaaa ttttcgacgg aaaaccacgt aaaaaacgtc gatttttcaa
     3721 gatacagcgt gaattttcag gaaatgcggt gagcatcaca tcaccacaat tcagcaaatt
     3781 gtgaacatca tcacgttcat ctttccctgg ttgccaatgg cccattttcc tgtcagtaac
     3841 gagaaggtcg cgaattcagg cgctttttag actggtcgta atgaaattca actaggtcgc
     3901 gccccgcacg ggcgcgtgga ttgaaacgag acctctctgg acaaaggtct cgtcgcccgg
     3961 caaaaccggg cgtggattga aacctagtgc tctgcaggaa ttcctcgaga agggagcgca
     4021 cctatggacg cggaggctag cgatactcac ttttttgctc actccacctt aaaggcagat
     4081 cgcagcgatt ggcagcctct ggtcgagcat ctacaggctg ttgcccgttt ggcaggagag
     4141 aaggctgcct tcttcggcgg cggtgaatta gctgctcttg ctggtctgtt gcatgacttg
     4201 ggtaaataca ctgacgagtt tcagcggcgt attgcgggtg atgccatccg tgtcgatcac
     4261 tctactcgcg gggccatact ggcggtagaa cgctatggcg cgctaggtca attgctagcc
     4321 tacggcatcg ctggccacca tgccgggttg gccaatggcc gcgaggctgg tgagcgaact
     4381 gccttggtcg accgcctgaa aggggttggg ctgccacggt tattggaggg gtggtgcgtg
     4441 gaaatcgtgc tacccgagcg ccttcaacca ccgccactaa aagcgcgcct ggaaagaggt
     4501 ttctttcagt tggcctttct tggccggatg ctcttttcct gcttggttga tgcggattat
     4561 ctagataccg aagccttcta ccaccgcgtc gaaggacggc gctcccttcg cgagcaagcg
     4621 cggccgacct tggccgagtt acgcgcagcc cttgatcggc atctgactga gttcaaggga
     4681 gatacgccgg tcaaccgcgt tcgcggggag atattggccg gcgtgcgcgg caaggcgagc
     4741 gaacttcccg ggctgttttc tctcacagtg cccacaggag gcggcaagac cctggcctct
     4801 ctggctttcg ccctggatca cgctctagct catgggctgc gccgggtgat ctacgtgatt
     4861 cccttcacta gcatcgtcga gcagaacgct gcggtattcc gtcgtgcact cggggcctta
     4921 ggcgaagagg cggtgctgga gcatcacagc gccttcgttg atgaccgccg gcagagcctg
     4981 gaggccaaga agaaactgaa cctagcgatg gagaactggg acgcgcctat cgtggtgacc
     5041 actgcagtgc agttcttcga aagcctgttt gccgaccgtc cagcccagtg ccgcaagcta
     5101 cacaacatcg ccggcagcgt ggtgattctt gacgaggcac agaccctacc gctcaagctg
     5161 ttgcggccct gcgttgccgc ccttgatgaa ctggcgctca actaccgttg tagcccagtt
     5221 ctctgtactg ccacgcagcc agcgcttcaa tcgccggatt tcatcggtgg gctgcaggac
     5281 gtacgtgagc tggcgcccga gccgcagcgg ctgttccggg agttggtgcg ggtacgaata
     5341 cggacattgg gcccgctcga agatgcggcc ttgactgagc agatcgccag gcgtgaacaa
     5401 gtgctgtgca tcgtcaacaa tcgacgccag gcccgtgcgc tctatgagtc gcttgccgag
     5461 ttgcccggtg cccgccatct caccaccctg atgtgcgcca agcaccgtag cagcgtgctg
     5521 gccgaggtgc gccagatgct caaaaagggg gagccctgtc gcctggtggc cacctcgctg
     5581 atcgaggccg gtgtggatgt ggattttccc gtggtactgc gtgccgaggc tggattggat
     5641 tccatcgccc aggccgcggg acgctgcaat cgcgaaggca agcggccgct ggccgaaagc
     5701 gaggtgctgg tgttcgccgc ggccaattct gactgggcgc cacccgagga actcaagcag
     5761 ttcgcccagg ccgcccgcga agtgatgcgc ctgcacccgg atgattgcct gtccatggcg
     5821 gccatcgagc ggtattttcg catactgtac tggcagaagg gcgcggagga gttggatgcg
     5881 ggtaacctgc tcggcctgat tgagagaggc cggctcgatg gcctgcccta cgagactttg
     5941 gccaccaagt tccgcatgat cgacagcctt caactgccgg tgatcatccc atttgatgac
     6001 gaggccagag cagccctgcg cgagctggag ttcgccgacg gctgcgccgc catcgcccgt
     6061 cgcctgcagc catatctggt gcagatgcca cgcaagggtt atcaggcatt gcgggaagcc
     6121 ggtgcgatcc aggcggcggc aggtacgcgt tatggtgagc agtttatggc gttggtcaac
     6181 cctgatctgt atcaccacca attcgggttg cactgggata atccggcctt tgtcagcagc
     6241 gagcggctat gttggtagtc gggacgcgca acagcggcct ggcctggatg atgtgaaagg
     6301 gagggccgat ggcctacgga attcgcttaa tggtctgggg cgagcgtgcc tgcttcaccc
     6361 gcccggaaat gaaggtggaa cgcgtctctt acgatgcgat cacgccgtcc gccgcgcgcg
     6421 gcattctcga ggctatccac tggaagccgg cgattcgctg ggtggtggat cgcattcaag
     6481 tgcttaagcc gatccgcttc gaatccatcc ggcgcaacga ggtcggcggc aagctgtccg
     6541 ctgtcagcgt cggtaaggca atgaaggccg ggcgtactaa tggtctggtg aatctggtcg
     6601 aggaggatcg ccagcagcgc gcgactactc tgctgcgcga tgtctcctat gtcatcgagg
     6661 cgcatttcga gatgactgac agggctggcg ccgacgatac ggtgggcaag catctggata
     6721 tcttcaaccg tcgcgcacgg aaggggcagt gcttccatac accctgccta ggcgtgcgcg
     6781 agtttccggc cagttttcgg ttgctggaag agggcagtgc cgagcctgaa gtcgatgcct
     6841 ttctgcgcgg cgagcgtgat ctgggctgga tgctgcatga cattgacttc gccgatggca
     6901 tgaccccgca cttcttccgt gccctgatgc gcgatgggct gatcgaggtg ccggccttca
     6961 gggcggcaga ggacaaggca tgatcctttc ggccctcaat gactattatc agcgactgct
     7021 ggagcggggt gaagcgaata tctcaccctt cggctacagc caagaaaaga tcagttacgc
     7081 cctgctgctg tccgcacaag gagagttgct ggacgtgcag gacattcgct tgctctctgg
     7141 caagaagcct caacccaggc ttatgagtgt gccgcagccg gagaagcgca cctcgggcat
     7201 caagtccaac gtactgtggg acaagaccag ctatgtgctg ggtgttagtg ccaagggcgg
     7261 agagcgtact cagcaggagc acgagtcctt caagacgctg caccggcaga tcttggttgg
     7321 ggaaggcgac cccggtctgc aggccttgct ccagttcctc gactgttggc agccggagca
     7381 gttcaagccc ccgctgttca gcgaagcaat gctcgacagc aacttagtgt tccgcctaga
     7441 cggccaacaa cgctatctgc acgagactcc ggcggccctg gcgttgcgta cccggctgtt
     7501 ggccgacggc gacagccgcg aggggctgtg cctagtctgc ggccaacgtc agccgttggc
     7561 gcgcctgcat ccagcggtca agggcgtcaa tggtgcccag agttcggggg cttccatcgt
     7621 ctccttcaac ctcgacgctt tttcctccta cggcaagagc cagggggaaa atgctccggt
     7681 gtccgaacag gccgcctttg cctacaccac ggtgctcaac catttgttgc gtcgcgacga
     7741 gcacaaccgc cagcgcctgc agattggcga cgcgagtgtg gtgttctggg cgcaggcgga
     7801 tactcctgct caggtggccg ccgccgagtc gaccttctgg aacctgctgg agccacccgc
     7861 agatgatggt caggaagcgg aaaagctgcg cggcgtgctg gatgctgtgg ccacggggcg
     7921 gcccttgcat gagctcgact cgctaatgga ggaaggcacc cgcatttttg tgttagggct
     7981 ggcgcccaat acctcgcgac tgtccattcg gttctgggca gtcgatagcc ttgcggtatt
     8041 cacccagcat ctggccgagc atttccggga tatgcacctt gagcctctgc cctggaagac
     8101 ggagccggcc atctggcgct tgctctatgc taccgcgccc agtcgtgacg gcagagccaa
     8161 gaccgaagac ttactcccac aactggccgg tgaaatgacc cgcgccatcc tgaccggcag
     8221 ccgctatccg cgcagtttgc tagccaacct gatcatgcgc atgcgtgccg acggcgacgt
     8281 ctctggcata cgcgtcgcgc tgtgcaaggc cgtgctcgct cgcgaggcac gcctgagcgg
     8341 caaaattcac caagaggagc tacctatgag tctcgacaag gacgccagca accccggcta
     8401 tcgcttgggg aggctgttcg ccgtgttgga aggcgcccag cgcgcagccc tgggcgacag
     8461 ggtcaatgcc actatccgtg accgctacta cggtgccgcg tccagcacgc cagccacggt
     8521 tttcccgata ctgctgcgca acacacaaaa ccacttggcc aagctgcgca aggagaagcc
     8581 cggactagca gtgaacctag agcgcgatat aggcgaaatc attgacggta tgcagagcca
     8641 attcccgcgt tgcctgcgcc tggaggacca gggacgcttt gctattggtt actaccaaca
     8701 ggcccaggcc cgtttcaacc gtggccccga ttccgtcgag taaggagcag aagaatgacc
     8761 gccatctcca accgctacga gttcgtttac ctctttgatg tcagcaatgg caatcccaat
     8821 ggcgacccgg atgctggcaa catgccgcgt ctcgatccgg aaaccaacca ggggttggtc
     8881 actgacgttt gcctcaagcg caagatccgc aactacgtca gcctggagca ggaaagtgcc
     8941 cccggctatg ccatctatat gcaggaaaaa tccgtgctga ataaccagca caaacaggcc
     9001 tacgaggcgc tcggtatcga gtcagaggca aagaaactgc ccaaggacga agccaaggcg
     9061 cgcgaactga cctcttggat gtgcaagaac ttcttcgatg tgcgtgcttt cggggcggtg
     9121 atgaccaccg agattaatgc cggccaggtg cgtggaccga tccaactggc attcgccacg
     9181 tctatcgacc cggtattgcc tatggaggta tccatcaccc gcatggcggt gactaacgaa
     9241 aaggatttgg agaaggaacg caccatggga cgcaagcaca tcgtgcctta cggcttgtac
     9301 cgcgcccatg gtttcatctc tgccaagttg gccgagcgaa ccggcttttc cgacgacgac
     9361 ttggaactgc tatggcgcgc tttggccaat atgttcgaac acgaccgctc ggcggcacgt
     9421 ggcgagatgg cagcgcgcaa gttgatcgtc ttcaagcatg agcatgccat gggcaatgca
     9481 cccgcccatg tgctgttcgg cagcgttaag gtcgagcgag tcgaggggga cgcagttaca
     9541 ccagcacgcg gtttccagga ttaccgtgtc agcatcgatg cggaagctct gcctcagggc
     9601 gtgagcgtgc gcgagtacct ctagtttcag gtatcgatac cgtcgacggg gattccttaa
     9661 ggtatacttt ccgctgcata accctgcttc ggggtcatta tagcgatttt ttcggtatat
     9721 ccatcctttt tcgcacgata tacaggattt tgccaaaggg ttcgtgtaga ctttccttgg
     9781 tgtatccaac ggcgtcagcc gggcaggata ggtgaagtag gcccacccgc gagcgggtgt
     9841 tccttcttca ctgtccctta ttcgcacctg gcggtgctca acgggaatcc tgctctgcga
     9901 ggctggccga taagctagct tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     9961 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
    10021 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
    10081 tggaggctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
    10141 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
    10201 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
    10261 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
    10321 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
    10381 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
    10441 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
    10501 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
    10561 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
    10621 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
    10681 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
    10741 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
    10801 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
    10861 tgctgcaatg ataccgcgtg acctacgctc accggctcca gatttatcag caataaacca
    10921 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
    10981 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
    11041 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
    11101 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    11161 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    11221 ggttatggca gcactgcata atcctcttac tgtcatgcca tccgtaagat gcttttctgt
    11281 gactggtgag tcgaattggc cgcggcgttg tgacaattta ccgaacaact ccgcggccgg
    11341 gaagccgatc tcggcttgaa cgaattgtta ggtggcggta cttgggtcga tatcaaagtg
    11401 catcacttct tcccgtatgc ccaactttgt atagagagcc actgcgggat cgtcaccgta
    11461 atctgcttgc acgtagatca cataagcacc aagcgcgttg
//