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pcDNA3.1+N-HA载体质粒基本信息
pcDNA3.1+N-HA质粒图谱载体图谱和pcDNA3.1+N-HA载体序列质粒序列多克隆位点信息
pcDNA3.1+N-HA质粒载体简介
pcDNA3.1+N-HA质粒序列载体序列
LOCUS pcDNA3.1+ N-HA 5450 bp ds-DNA circular SYN 21-MAY-2015
DEFINITION Mammalian vector with a CMV promoter for expressing N-terminally
HA-tagged proteins.
ACCESSION .
VERSION .
KEYWORDS pcDNA3.1+ N-HA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5450)
AUTHORS GenScript
TITLE Direct Submission
JOURNAL Exported Tuesday, February 4, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5450
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
enhancer 235..614
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 863..881
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 923..925
/codon_start=1
/product="start codon"
/note="ATG"
/translation="M"
CDS 926..952
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/note="HA"
/translation="YPYDVPDYA"
misc_feature 953..1021
/note="MCS"
polyA_signal 1047..1271
/note="bGH poly(A) signal"
/note="bovine growth hormone polyadenylation signal"
rep_origin 1317..1745
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 1759..2088
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 1939..2074
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 2155..2949
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 3123..3244
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(3695..4283)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4454..5314)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5315..5419)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagcttgcca ccatgtaccc atacgatgtt ccagattacg ctggtaccga
961 gctcggatcc gaattctgca gatatccagc acagtggcgg ccgctcgagt ctagagggcc
1021 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
1081 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
1141 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
1201 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
1261 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
1321 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
1381 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
1441 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
1501 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
1561 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
1621 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
1681 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
1741 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
1801 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
1861 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
1921 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
1981 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
2041 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
2101 gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt
2161 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
2221 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
2281 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
2341 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
2401 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
2461 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
2521 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
2581 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
2641 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
2701 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
2761 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
2821 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
2881 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
2941 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
3001 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
3061 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
3121 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
3181 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
3241 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
3301 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
3361 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
3421 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
3481 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
3541 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
3601 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
3661 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
3721 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
3781 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
3841 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
3901 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
3961 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
4021 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
4081 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
4141 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
4201 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
4261 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
4321 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
4381 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
4441 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
4501 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
4561 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
4621 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
4681 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
4741 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
4801 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
4861 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
4921 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
4981 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
5041 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
5101 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
5161 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
5221 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
5281 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
5341 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
5401 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//
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