LOCUS pREDIT_MCP-RecT 10549 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION mammalian-optimized expression of SSAP RecT fused to MCP (for MS2
aptamer), pLenti-EF1A-MCP-EXTEN-RecT-NLS.
ACCESSION .
VERSION .
KEYWORDS pREDIT_MCP-RecT
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10549)
TITLE Microbial Single-strand Annealing Proteins Enable CRISPR
Gene-editing Tools with Improved Knock-in Efficiencies and Reduced
Off-target Effects
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 10549)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10549
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(47..66)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 238..617
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 619..817
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 772..792
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 835..1015
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 1062..1187
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1680..1913
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 2098..2142
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2440..2557
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2682..3860
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 2913..3851
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 3808..3828
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
regulatory 3867..3876
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 3876..4262
/codon_start=1
/product="bacteriophage MS2 coat protein"
/label=MS2-N55K
/note="binds with high affinity to a specific stem-loop
structure in the viral RNA (Lim et al., 1994)"
/translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
VKAMQGLLKDGNPIPSAIAANSGIY"
CDS 5193..5213
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
misc_feature 5241..5829
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(5294..5314)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5712..5723)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(5832..5848)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5833..5849)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 6354..6534
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
primer_bind complement(6560..6577)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 6566..6790
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 6836..7264
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6923..6942)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 7133..7154
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(7273..7293)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 7278..7607
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 7458..7593
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 7520..7539
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 7655..7702
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 7721..8095
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 8225..8346
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(8262..8281)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 8316..8335
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(8395..8411)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8395..8411)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(8408..8430)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 8419..8435
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(8443..8473)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8488..8509
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(8626..8643)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8797..9385)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8877..8896)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(9556..10416)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 10179..10198
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(10417..10521)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
2581 agatccagtt tggttaatta atgcaaagat ggataaagtt ttaaacagag aggaatcttt
2641 gcagctaatg gaccttctag gtcttgaaag gagtgggaat tggctccggt gcccgtcagt
2701 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
2761 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
2821 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
2881 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
2941 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
3001 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
3061 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
3121 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
3181 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
3241 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
3301 gtgcgcccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
3361 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
3421 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
3481 ggccgcttcc cggccctgct gcagggagct caaaatgaag gacgcggcgc tcgggagagc
3541 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
3601 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
3661 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
3721 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
3781 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
3841 ttccatttca ggtgtcgtga cgtacggcca ccatggcttc aaactttact cagttcgtgc
3901 tcgtggacaa tggtgggaca ggggatgtga cagtggctcc ttctaatttc gctaatgggg
3961 tggcagagtg gatcagctcc aactcacgga gccaggccta caaggtgaca tgcagcgtca
4021 ggcagtctag tgcccagaag agaaagtata ccatcaaggt ggaggtcccc aaagtggcta
4081 cccagacagt gggcggagtc gaactgcctg tcgccgcttg gaggtcctac ctgaacatgg
4141 agctcactat cccaattttc gctaccaatt ctgactgtga actcatcgtg aaggcaatgc
4201 aggggctcct caaagacggt aatcctatcc cttccgccat cgccgctaac tcaggtatct
4261 acagcgcttc cggaggatct agcggaggct cctctggctc tgagacacct ggcacaagcg
4321 agagcgcaac acctgaaagc agcgggggca gcagcggggg gtcaggagga tccatgacaa
4381 aacaacctcc aatagcaaaa gctgatctgc agaaaaccca gggcaataga gcccctgctg
4441 ctgtgaagaa tagtgacgtg attagcttca tcaaccagcc tagcatgaag gagcagctgg
4501 ccgccgccct gcctcgccac atgaccgcag agcggatgat tcggatcgct acaaccgaga
4561 tcagaaaagt gcccgctctg gggaactgtg ataccatgtc tttcgtgagc gccattgtgc
4621 agtgtagcca gctgggcctg gagcccggaa gtgccctggg ccacgcctac ctgctgccct
4681 tcggcaacaa gaacgagaaa tccggtaaaa agaacgtgca gctgatcatc ggttatagag
4741 gtatgatcga tctggctcgg cggtccggcc aaatcgccag cctgtctgcc agggtggtga
4801 gagagggcga cgagttcagc tttgagtttg gcctggacga aaagttgatc catcggcccg
4861 gagagaacga ggacgcccct gtcacccacg tgtatgccgt ggctagactg aaggacggcg
4921 gtacacagtt cgaagttatg acccggaagc agatcgaact ggtgaggtcc ctgtccaagg
4981 ccggcaataa cgggccctgg gtgacccact gggaagaaat ggccaagaaa accgccatta
5041 gacgcctgtt caagtacctg cccgtgtcca ttgagattca gagggccgtg tctatggatg
5101 agaaagagcc cctgaccatc gaccctgctg acagcagcgt gctgaccggc gagtactctg
5161 tgattgacaa ttctgaggag tctggtggtt ctcctaagaa aaagaggaag gtgtgagaat
5221 tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
5281 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
5341 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
5401 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
5461 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
5521 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
5581 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
5641 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
5701 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
5761 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
5821 cctccccgca tcgataccgt cgacctcgag acctagaaaa acatggagca atcacaagta
5881 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg
5941 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag
6001 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
6061 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga
6121 actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag
6181 taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac
6241 accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg
6301 acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc
6361 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
6421 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
6481 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc
6541 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
6601 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
6661 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
6721 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
6781 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
6841 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
6901 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
6961 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
7021 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
7081 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
7141 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
7201 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
7261 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
7321 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
7381 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
7441 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
7501 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
7561 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
7621 cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta
7681 tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc
7741 cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct
7801 cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac
7861 cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg
7921 ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg
7981 ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc
8041 cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt
8101 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
8161 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
8221 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
8281 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
8341 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
8401 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
8461 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
8521 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
8581 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
8641 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
8701 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
8761 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
8821 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
8881 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
8941 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
9001 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
9061 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
9121 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
9181 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
9241 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
9301 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
9361 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
9421 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
9481 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
9541 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
9601 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
9661 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
9721 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
9781 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
9841 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
9901 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
9961 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
10021 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
10081 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
10141 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
10201 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
10261 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
10321 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
10381 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
10441 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
10501 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgac
//
