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LOCUS pMXs-IRES-BFP 5942 bp DNA circular SYN 12-JUL-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5942)
AUTHORS Biofeng
TITLE Direct Submission
JOURNAL Exported Jul 12, 2023 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5942
/mol_type="other DNA"
/organism="synthetic DNA construct"
LTR 22..615
/note="long terminal repeat from Moloney murine leukemia
virus"
misc_feature 678..1035
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
CDS 1094..1510
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
misc_feature 1520..1894
/label=pol region
/note="Moloney murine leukemia virus (MMLV) pol region
containing the splice acceptor site"
misc_feature 2015..2566
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
CDS 2578..3276
/codon_start=1
/product="monomeric blue fluorescent protein"
/label=TagBFP
/note="mammalian codon-optimized"
/translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
DLPSKLGHKLN"
LTR 3447..3916
/note="long terminal repeat from Moloney murine leukemia
virus"
rep_origin complement(4217..4805)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4969..5829)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5830..5934)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc
481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg
781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc
841 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg
901 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct
961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc
1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa
1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
1501 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa
1561 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca
1621 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca
1681 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg
1741 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac
1801 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc
1861 tgccgacccc gggggtggac catcctctag actgccggat ctagctagtt aattaaggat
1921 cccagtgtgg tggtacggga attcctgcag gcctcgaggg ccggcgcgcc gcggccgcta
1981 cgtaaattcc gccccccccc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa
2041 taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttgcaatg
2101 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
2161 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
2221 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
2281 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
2341 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
2401 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
2461 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc
2521 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataatatggc cacaaccatg
2581 agcgagctga ttaaggagaa catgcacatg aagctgtaca tggagggcac cgtggacaac
2641 catcacttca agtgcacatc cgagggcgaa ggcaagccct acgagggcac ccagaccatg
2701 agaatcaagg tggtcgaggg cggccctctc cccttcgcct tcgacatcct ggctactagc
2761 ttcctctacg gcagcaagac cttcatcaac cacacccagg gcatccccga cttcttcaag
2821 cagtccttcc ctgagggctt cacatgggag agagtcacca catacgaaga cgggggcgtg
2881 ctgaccgcta cccaggacac cagcctccag gacggctgcc tcatctacaa cgtcaagatc
2941 agaggggtga acttcacatc caacggccct gtgatgcaga agaaaacact cggctgggag
3001 gccttcaccg agacgctgta ccccgctgac ggcggcctgg aaggcagaaa cgacatggcc
3061 ctgaagctcg tgggcgggag ccatctgatc gcaaacatca agaccacata tagatccaag
3121 aaacccgcta agaacctcaa gatgcctggc gtctactatg tggactacag actggaaaga
3181 atcaaggagg ccaacaacga gacctacgtc gagcagcacg aggtggcagt ggccagatac
3241 tgcgacctcc ctagcaaact ggggcacaag cttaatgtcg acgataaaat aaaagatttt
3301 atttagtctc cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc
3361 ttaagtaacg ccattttgca aggcatggaa aaatacataa ctgagaatag agaagttcag
3421 atcaaggtca ggaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag
3481 cagttcctgc cccggctcag ggccaagaac agatggaaca gctgaatatg ggccaaacag
3541 gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg gtccccagat
3601 gcggtccagc cctcagcagt ttctagagaa ccatcagatg tttccagggt gccccaagga
3661 cctgaaatga ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc
3721 gcgcgcttct gctccccgag ctcaataaaa gagcccacaa cccctcactc ggggcgccag
3781 tcctccgatt gactgagtcg cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc
3841 atccgacttg tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccgt
3901 cagcgggggt ctttcacatg cagcatgtat caaaattaat ttggtttttt ttcttaagta
3961 tttacattaa atggccatag ttgcattaat gaatcggcca acgcgcgggg agaggcggtt
4021 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
4081 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
4141 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
4201 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
4261 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
4321 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
4381 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
4441 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
4501 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
4561 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
4621 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
4681 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
4741 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
4801 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
4861 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttgcggc
4921 cggccgcaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
4981 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
5041 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
5101 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
5161 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
5221 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
5281 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
5341 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
5401 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
5461 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
5521 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
5581 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
5641 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
5701 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
5761 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
5821 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
5881 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
5941 tc
//
