pkk232-8

价格:1500元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Promega
载体名称: pkk232-8
质粒类型: 大肠杆菌表达载体
高拷贝/低拷贝: --
启动子: --
克隆方法: 多克隆位点,限制性内切酶
载体大小: 5094 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: Ampicillin
筛选标记: --
备注: --
产品目录号: --
稳定性: --
组成型: --
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pkk232-8载体图谱



载体简介

载体序列

LOCUS       pKK232-8	5094 bp 	DNA	SYN
DEFINITION  pKK232-8
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..5094
                     /organism="pKK232-8"
                     /mol_type="other DNA"
     terminator      15..58
                     /label="rrnB_T1_terminator"
     gene            269..928
                     /label="CAT/CamR"
                     /gene="CAT/CamR"
     CDS             269..928
                     /label="ORF frame 2"
                     /translation="MEKKITGYTTVDISQSHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
     misc_feature    complement(1193..1210)
                     /label="pBAD_rev_primer"
                     /translation="MEKKITGYTTVDISQSHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
     misc_feature    complement(1193..1210)
                     /label="pTrcHis_rev_primer"
                     /translation="MEKKITGYTTVDISQSHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
     terminator      1243..1400
                     /label="rrnB_terminator"
                     /translation="MEKKITGYTTVDISQSHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
     terminator      1366..1409
                     /label="rrnB_T1_terminator"
                     /translation="MEKKITGYTTVDISQSHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
     terminator      1541..1568
                     /label="rrnB_T2_terminator"
                     /translation="MEKKITGYTTVDISQSHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
     misc_feature    complement(1691..1708)
                     /label="pTrcHis_rev_primer"
                     /translation="MEKKITGYTTVDISQSHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
     misc_feature    complement(1691..1708)
                     /label="pBAD_rev_primer"
                     /translation="MEKKITGYTTVDISQSHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
     terminator      1741..1898
                     /label="rrnB_terminator"
                     /translation="MEKKITGYTTVDISQSHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
     terminator      1864..1907
                     /label="rrnB_T1_terminator"
                     /translation="MEKKITGYTTVDISQSHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
     terminator      2039..2066
                     /label="rrnB_T2_terminator"
                     /translation="MEKKITGYTTVDISQSHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
     misc_feature    2648..2839
                     /label="ROP"
                     /translation="MEKKITGYTTVDISQSHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
     misc_feature    complement(2833..2855)
                     /label="pGEX_3_primer"
                     /translation="MEKKITGYTTVDISQSHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
     rep_origin      complement(3252..3871)
                     /label="pBR322_origin"
                     /translation="MEKKITGYTTVDISQSHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
     gene            complement(4026..4886)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MEKKITGYTTVDISQSHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
     CDS             complement(4026..4886)
                     /label="ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(4928..4956)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 ttcccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
   61 gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgaacg
  121 ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact gccagggaat
  181 tcccggggat ccgtcgacct gcagccaagc ttgagtagga caaatccgcc gagcttcgac
  241 gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt
  301 tgatatatcc caatcgcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg
  361 tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa
  421 taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc
  481 ggaattccgt atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg
  541 ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga
  601 cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct
  661 ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt
  721 gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt
  781 caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt
  841 tcatcatgcc gtctgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta
  901 ctgcgatgag tggcagggcg gggcgtaatt tttttaaggc agttattggt gcccttaaac
  961 gcctggtgct acgcctgaat aagtgataat aagcggatga atggcagaaa ttcgtcgagg
 1021 cggcacctcg ctaacggatt caccactcca agaattggag ccaatcaatt cttgcggaga
 1081 actgtgaatg cgcaaaccaa cccttggcag aacatatcca tcgcgtccgc catctccagc
 1141 agccgcacgc ggcgcatctc ggctgttttg gcggatgaga gaagattttc agcctgatac
 1201 agattaaatc agaacgcaga agcggtctga taaaacagaa tttgcctggc ggcagtagcg
 1261 cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc gccgatggta
 1321 gtgtggggtc tccccatgcg agagtaggga actgccaggc atcaaataaa acgaaaggct
 1381 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
 1441 aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg agggtggcgg
 1501 gcaggacgcc cgccataaac tgccaggcat caaattaagc agaaggccat cctgacggat
 1561 ggcctttttg cgtttctaca aactcttcct gtcgtcatat ctacaagcca tccccccaca
 1621 gatacggtaa actagcctcg tttttgcatc aggaaagcag ctgttttggc ggatgagaga
 1681 agattttcag cctgatacag attaaatcag aacgcagaag cggtctgata aaacagaatt
 1741 tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat gccgaactca gaagtgaaac
 1801 gccgtagcgc cgatggtagt gtggggtctc cccatgcgag agtagggaac tgccaggcat
 1861 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
 1921 gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa
 1981 cggcccggag ggtggcgggc aggacgcccg ccataaactg ccaggcatca aattaagcag
 2041 aaggccatcc tgacggatgg cctttttgcg tttctacaaa ctcttcctgt cgtcatatct
 2101 acaagccatc cccccacaga tacggtaaac tagcctcgtt tttgcatcag gaaagcagtc
 2161 gggcagcgtt gggtcctggc cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg
 2221 gctaggctgg cggggttgcc ttactggtta gcagaatgaa tcaccgatac gcgagcgaac
 2281 gtgaagcgac tgctgctgca aaacgtctgc gacctgagca acaacatgaa tggtcttcgg
 2341 tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg
 2401 gatctgcatc gcaggatgct gctggctacc ctgtggaaca cctacatctg tattaacgaa
 2461 gcgctggcat tgaccctgag tgatttttct ctggtcccgc cgcatccata ccgccagttg
 2521 tttaccctca caacgttcca gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag
 2581 catcctctct cgtttcatcg gtatcattac ccccatgaac agaaatcccc cttacacgga
 2641 ggcatcagtg accaaacagg aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca
 2701 gacattaacg cttctggaga aactcaacga gctggacgcg gatgaacagg cagacatctg
 2761 tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc tgcctcgcgc gtttcggtga
 2821 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
 2881 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
 2941 cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca
 3001 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
 3061 aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
 3121 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
 3181 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
 3241 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
 3301 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
 3361 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
 3421 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat
 3481 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
 3541 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
 3601 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
 3661 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
 3721 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
 3781 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
 3841 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
 3901 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
 3961 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
 4021 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
 4081 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
 4141 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
 4201 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
 4261 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
 4321 cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct
 4381 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
 4441 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
 4501 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
 4561 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
 4621 agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa
 4681 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
 4741 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
 4801 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
 4861 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
 4921 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
 4981 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
 5041 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaa

//

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