| 质粒名称: | CHRM2-Tango |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 66249 |
| 存储实验室: | Bryan Roth |
|
载体骨架基本信息 |
|
| 载体名称: | empty Tango |
| 载体出品公司: | Clontech |
| 空载体大小: | 6632bp |
| 完整质粒大小: | 8033bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | CHRM2 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 1398bp |
| 启动子: | CMV |
| 标签/融合蛋白: | FLAG (N terminal on backbone) |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | NotI (not destroyed) |
| 3’克隆位点: | XhoI (unknown if destroyed) |
| 5’测序引物 | CMV-F |
|
文献引用方法 |
|
| 材料和方法部分: |
CHRM2-Tango was a gift from Bryan Roth (Addgene plasmid # 66249 ; http://n2t.net/addgene:66249 ; RRID:Addgene_66249)
|
| 参考文献部分: | PRESTO-Tango as an open-source resource for interrogation of the druggable human GPCRome. Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, Sciaky N, Roth BL. Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. Epub 2015 Apr 20. 10.1038/nsmb.3014 PubMed 25895059 |
买家导航
CHRM2-Tango载体质粒基本信息
CHRM2-Tango质粒图谱载体图谱和CHRM2-Tango载体序列质粒序列多克隆位点信息
CHRM2-Tango质粒载体简介
CHRM2-Tango质粒序列载体序列
LOCUS CHRM2-Tango 8033 bp DNA circular SYN 14-APR-2025
DEFINITION Expression of G protein-coupled receptors for PRESTO-Tango: parallel
receptorome expression and screening via transcriptional output,
with transcriptional activation following arrestin translocation.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8033)
AUTHORS Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM,
Sciaky N, Roth BL
TITLE PRESTO-Tango as an open-source resource for interrogation of the
druggable human GPCRome.
JOURNAL Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014.
Epub 2015 Apr 20.
PUBMED 25895059
REFERENCE 2 (bases 1 to 8033)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3014";
journalName: "Nat Struct Mol Biol"; date: "2015-05"; volume: "22";
issue: "5"; pages: "362-9"
COMMENT Sequence Label: CHRM2-Tango
FEATURES Location/Qualifiers
source 1..8033
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 1..380
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 381..584
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 535..555
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 629..648
/label=T7
/note="T7 promoter, forward primer"
promoter 629..647
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS join(2357..2971,2972..3358)
/codon_start=1
/product="tetracycline-controlled transactivator,
comprising a fusion of the tetracycline repressor TetR with
the C-terminal activation domain of herpes simplex virus
VP16"
/label=tTA
/note="In the Tet-Off(R) system, tTA binds to the
Tet-responsive element and stimulates transcription only in
the absence of tetracycline or doxycycline."
/translation="RLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVKN
KRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRPT
EKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTDS
MPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGSTIEG
LLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHADA
LDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
primer_bind complement(2424..2443)
/label=Tet-R
/note="Tetracycline resistance gene, reverse primer"
primer_bind complement(3390..3407)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 3396..3620
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 3666..4094
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3753..3772)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 3963..3984
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(4103..4123)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 4108..4437
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 4288..4423
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 4350..4369
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 4504..5298
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(4558..4577)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 5168..5187
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 5472..5593
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5509..5528)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5563..5582
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(5642..5658)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5642..5658)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5655..5677)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5666..5682
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(join(5690..5696,5697..5714,5715..5720))
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5735..5756
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(5873..5890)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6044..6632)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6124..6143)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(6803..7594,7595..7663))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7426..7445
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7664..7768)
/gene="bla"
/label=AmpR promoter
primer_bind complement(7843..7862)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
661 gctagcgttt aaacttaagc ttggtaccga gctcggatcc actagtccag tgtggtggaa
721 ttctgcagat atccagcaca gtggcggccg cgccaccatg aagacgatca tcgccctgag
781 ctacatcttc tgcctggtat tcgccgacta caaggacgat gatgacgcca gcatcgatat
841 gaacaatagt actaatagct caaacaactc attggccctc acaagccctt acaagacctt
901 tgaggttgtc ttcattgtgc tggtggctgg atcactgtcc ctggtaacca tcatcggcaa
961 tatccttgtg atggtgtcta taaaggtcaa taggcacttg cagacagtca acaactattt
1021 cctgttctca ttggcatgcg cagatttgat aataggcgta ttctccatga acctttatac
1081 actgtatact gtcattggat attggccatt gggccccgtg gtctgcgacc tttggcttgc
1141 actcgactac gttgtttcca atgcatctgt aatgaacctg ctgattatct cattcgatcg
1201 gtacttctgc gttaccaagc ccctgactta cccagtgaag aggactacaa agatggccgg
1261 aatgatgatt gcagcagctt gggtgctttc ttttatactg tgggctcccg ctattctgtt
1321 ctggcagttt attgtgggtg taaggactgt ggaagacggc gagtgctata tccagttctt
1381 ctctaacgca gctgtaacct tcgggactgc catcgctgcc ttctacctgc ccgtcatcat
1441 tatgacagtg ctgtattggc atatctcaag agctagcaag tcccggataa aaaaggacaa
1501 gaaagagcca gttgcaaatc aagaccccgt ctcaccttcc cttgtgcagg ggagaatcgt
1561 caaaccaaat aataacaata tgccgagctc cgacgacggc ctggaacata acaaaattca
1621 gaatgggaaa gcaccaaggg accccgtgac cgagaattgc gtgcaggggg aggagaaaga
1681 gagttctaac gactctacct ctgtcagcgc cgtggcttcc aatatgcggg atgatgaaat
1741 tactcaggat gaaaatactg tttccacatc cttgggccac agcaaggacg aaaactccaa
1801 gcaaacatgc attcgcattg ggaccaagac acctaaatcc gacagttgca ctccgacaaa
1861 cacaactgtc gaagtggtgg gttcctcagg ccagaacgga gacgagaagc agaacattgt
1921 tgcccggaag atcgtgaaga tgaccaaaca gcccgctaag aaaaagcctc cccccagtcg
1981 cgaaaaaaag gtgactagaa ctatcctcgc tatcctcctg gcattcatta tcacatgggc
2041 cccatacaat gttatggtac ttatcaacac tttctgcgct ccctgtatcc ccaacactgt
2101 ttggactatc ggctactggc tgtgttatat taacagtaca atcaaccctg catgctatgc
2161 cctgtgtaat gccacattca aaaaaacatt taagcacctc ctgatgtgtc actacaaaaa
2221 cataggcgcg acacgcatcg ataccggtgg acgcacccca cccagcctgg gtccccaaga
2281 tgagtcctgc accaccgcca gctcctccct ggccaaggac acttcatcga ccggtgagaa
2341 cctgtacttc cagctaagat tagataaaag taaagtgatt aacagcgcat tagagctgct
2401 taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc taggtgtaga
2461 gcagcctaca ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg ccttagccat
2521 tgagatgtta gataggcacc atactcactt ttgcccttta gaaggggaaa gctggcaaga
2581 ttttttacgt aataacgcta aaagttttag atgtgcttta ctaagtcatc gcgatggagc
2641 aaaagtacat ttaggtacac ggcctacaga aaaacagtat gaaactctcg aaaatcaatt
2701 agccttttta tgccaacaag gtttttcact agagaatgca ttatatgcac tcagcgctgt
2761 ggggcatttt actttaggtt gcgtattgga agatcaagag catcaagtcg ctaaagaaga
2821 aagggaaaca cctactactg atagtatgcc gccattatta cgacaagcta tcgaattatt
2881 tgatcaccaa ggtgcagagc cagccttctt attcggcctt gaattgatca tatgcggatt
2941 agaaaaacaa cttaaatgtg aaagtgggtc cgcgtacagc cgcgcgcgta cgaaaaacaa
3001 ttacgggtct accatcgagg gcctgctcga tctcccggac gacgacgccc ccgaagaggc
3061 ggggctggcg gctccgcgcc tgtcctttct ccccgcggga cacacgcgca gactgtcgac
3121 ggcccccccg accgatgtca gcctggggga cgagctccac ttagacggcg aggacgtggc
3181 gatggcgcat gccgacgcgc tagacgattt cgatctggac atgttggggg acggggattc
3241 cccgggtccg ggatttaccc cccacgactc cgccccctac ggcgctctgg atatggccga
3301 cttcgagttt gagcagatgt ttaccgatgc ccttggaatt gacgagtacg gtgggtagtc
3361 tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
3421 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
3481 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
3541 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
3601 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
3661 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
3721 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
3781 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
3841 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
3901 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
3961 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
4021 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
4081 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
4141 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
4201 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
4261 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
4321 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
4381 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
4441 ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt
4501 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
4561 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
4621 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
4681 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
4741 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
4801 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
4861 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
4921 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
4981 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc
5041 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
5101 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
5161 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
5221 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
5281 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca
5341 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
5401 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
5461 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
5521 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
5581 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
5641 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
5701 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
5761 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
5821 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
5881 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
5941 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
6001 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
6061 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
6121 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
6181 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
6241 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
6301 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
6361 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
6421 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
6481 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
6541 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
6601 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
6661 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
6721 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
6781 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
6841 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
6901 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
6961 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
7021 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
7081 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
7141 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
7201 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
7261 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
7321 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
7381 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
7441 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
7501 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
7561 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
7621 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
7681 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
7741 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
7801 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
7861 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
7921 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
7981 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gtt
//
CHRM2-Tango载体图谱质粒图谱pdf版和CHRM2-Tango载体序列质粒序列等相关资料下载
CHRM2-Tango质粒载体应用举例
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