| 质粒名称: | p45sheLL |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 37323 |
| 存储实验室: | John Schiller |
|
载体骨架基本信息 |
|
| 载体名称: | custom |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | HPV45L1+L2,p45LLw |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | p45sheLL was a gift from John Schiller (Addgene plasmid # 37323 ; http://n2t.net/addgene:37323 ; RRID:Addgene_37323) |
| 参考文献部分: | Genital transmission of HPV in a mouse model is potentiated by nonoxynol-9 and inhibited by carrageenan. Roberts JN, Buck CB, Thompson CD, Kines R, Bernardo M, Choyke PL, Lowy DR, Schiller JT. Nat Med. 2007 Jul;13(7):857-61. Epub 2007 Jul 1. 10.1038/nm1598 PubMed 17603495 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS p45sheLL 10814 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS p45sheLL
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10814)
AUTHORS Roberts JN, Buck CB, Thompson CD, Kines R, Bernardo M, Choyke PL,
Lowy DR, Schiller JT
TITLE Genital transmission of HPV in a mouse model is potentiated by
nonoxynol-9 and inhibited by carrageenan.
JOURNAL Nat Med. 2007 Jul;13(7):857-61. Epub 2007 Jul 1.
PUBMED 17603495
REFERENCE 2 (bases 1 to 10814)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10814
/organism="synthetic DNA construct"
/mol_type="other DNA"
regulatory 19..28
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
regulatory 2260..2269
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
protein_bind complement(3665..3689)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
misc_feature 3751..4339
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(3804..3824)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(4222..4233)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(4376..4393)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 4382..4606
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 4652..5080
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4739..4758)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4949..4970
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(5089..5109)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
CDS 7038..7406
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="KLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDDVT
LFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGREF
ALRDPAGNCVHFVAEEQD"
polyA_signal 7536..7657
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7573..7592)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 7627..7646
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(7706..7722)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7706..7722)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7719..7741)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7730..7746
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7754..7784)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7799..7820
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(7937..7954)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8108..8693)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8188..8207)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8864..9724)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9487..9506
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9725..9829)
/gene="bla"
/label=AmpR promoter
primer_bind complement(9904..9923)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 10095..10474
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 10475..10678
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 10629..10649
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
protein_bind 10680..10698
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 10701..10719
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
primer_bind 10729..10753
/label=LNCX
/note="Human CMV promoter, forward primer"
ORIGIN
1 tccgggggat ccactagagc caccatggcc ctctggagac cctccgattc caccgtgtac
61 ttgccccccc ccagcgtcgc acgcgtcgtg tctaccgacg actacgtcag caggacctca
121 atcttctacc acgccgggtc cagtaggctg ctgaccgtgg ggaaccccta cttccgcgtc
181 gtgcccaacg gcgccggcaa caagcaagcc gtccccaaag tcagtgccta ccagtaccgc
241 gtcttccgcg tggccctgcc agaccccaac aagttcggcc tgcccgacag caccatctac
301 aaccccgaga cccagaggct cgtctgggcc tgcgtgggca tggagatcgg caggggccaa
361 cccctgggca tcgggttgtc cgggcacccc ttctacaaca agctcgacga caccgagtcc
421 gcccacgccg ccaccgccgt catcacccag gacgtccgcg acaacgtcag cgtcgactac
481 aaacagaccc aactctgcat cctgggctgc gtgcccgcca tcggcgaaca ttgggcaaag
541 gggaccttgt gcaagcccgc ccagctccag cccggcgatt gcccccccct cgagttgaag
601 aatacaatca tcgaggacgg cgacatggtc gacaccggct acggcgccat ggacttctcc
661 accctccaag acaccaaatg tgaagtcccc ctggatatct gccagagtat ttgcaagtac
721 cccgactacc tccagatgag cgccgaccca tacggcgaca gcatgttctt ctgtttgagg
781 agggagcagc tcttcgcccg ccacttctgg aaccgcgccg gcgtcatggg cgataccgtg
841 cccaccgatt tgtacatcaa ggggacctca gccaacatga gggagacacc ggggtcctgc
901 gtctacagtc ccagcccatc cgggagcatc atcaccagcg acagccagct gttcaacaag
961 ccctactggc tgcacaaagc acaggggcac aataacggca tctgctggca caaccaactc
1021 ttcgtcaccg tggtcgatac cacaaggtcc accaacctga ccctgtgcgc aagcacccag
1081 aaccccgtcc cctccaccta cgatcccacc aagttcaaac agtactcccg ccacgtcgaa
1141 gagtacgacc tgcagttcat cttccaactc tgtaccatca ccctgaccgc cgaggtcatg
1201 agctacattc actccatgaa ctcctccatc ctggagaact ggaacttcgg cgtgcccccc
1261 ccccccacca cctccctcgt cgacacctac aggttcgtcc agagcgtcgc cgtcacatgc
1321 cagaaggaca ccaccccccc cgagaaacag gacccctacg acaagctgaa gttctggacc
1381 gtcgatttga aggagaagtt cagtagtgac ctcgaccagt acccattggg caggaaattc
1441 ctggtccaag ccggcctgag gaggcgcccc acaatcggcc ccaggaagag gcccgccgcc
1501 agtaccagca ccgccagcac cgccagccgc cccgcaaagc gcgtcaggat caggtccaag
1561 aaatgagccc ggtggatccc aatcaagctt tttgcaaaag cctagggctc gaggaagctt
1621 aaaacagctc tggggttgta cccaccccag aggcccacgt ggcggctagt actccggtat
1681 tgcggtaccc ttgtacgcct gttttatact cccttcccgt aacttagacg cacaaaacca
1741 agttcaatag aagggggtac aaaccagtac caccacgaac aagcacttct gtttccccgg
1801 tgatgtcgta tagactgctt gcgtggttga aagcgacgga tccgttatcc gcttatgtac
1861 ttcgagaagc ccagtaccac ctcggaatct tcgatgcgtt gcgctcagca ctcaacccca
1921 gagtgtagct taggctgatg agtctggaca tccctcaccg gtgacggtgg tccaggctgc
1981 gttggcggcc tacctatggc taacgccatg ggacgctagt tgtgaacaag gtgtgaagag
2041 cctattgagc tacataagaa tcctccggcc cctgaatgcg gctaatccca acctcggagc
2101 aggtggtcac aaaccagtga ttggcctgtc gtaacgcgca agtccgtggc ggaaccgact
2161 actttgggtg tccgtgtttc cttttatttt attgtggctg cttatggtga caatcacaga
2221 ttgttatcat aaagcgaatt ggattgcggc cgctctagag ccaccatggt cagtcatagg
2281 gccgccagga ggaagagagc aagcgccacc gatctgtacc gcacctgcaa acagagtggc
2341 acctgtccac ccgacgtcat caataaggtc gaggggacca cactggccga caagatcctg
2401 caatggagct cattgggcat cttcctcggc gggttgggga tcggcacagg gtccggcagc
2461 ggcgggagga ccggatacgt gccactgggc gggcgcagca acaccgtcgt cgacgtcggg
2521 ccaacccgcc cccccgtcgt catcgagccc gtgggcccca ccgaccccag catcgtcacc
2581 ctcgtggaag acagttccgt cgtcgcaagc ggcgcccccg tcccaacctt caccggcaca
2641 agcggcttcg agatcaccag cagcggcacc acaacccccg ccgtcctcga tattaccccc
2701 accgtcgata gcgtcagcat cagcagcacc tccttcacca acccagcctt cagcgaccca
2761 agcatcatcg aggtcccaca gaccggcgaa gtcagcggca acatcttcgt cggcaccccc
2821 accagcgggt ctcacggcta cgaagagatc ccactgcaga ccttcgccag cagcggcagc
2881 ggcaccgagc caatctcctc cacaccattg cccaccgtca gaagagtggc cggcccaagg
2941 ctctactccc gcgccaacca gcaagtcagg gtcagtacaa gccagttcct gacccaccca
3001 agcagcctcg tcaccttcga caaccccgcc tacgagccac tcgatacaac cttgagtttc
3061 gaacccacat ccaacgtccc cgacagtgac ttcatggaca tcatcaggct ccaccgcccc
3121 gccctgagta gccgcagggg gaccgtccgc ttctcccgcc tcggccagcg cgccacaatg
3181 ttcaccaggt ccggcaagca gatcggcggc cgcgtgcact tctatcacga catctctcca
3241 atcgccgcca ccgaagagat cgagctccaa cccctgatct ccgccaccaa cgactccgat
3301 ctcttcgacg tgtacgccga ttttccgcca cccgccagta ccaccccctc aaccatccat
3361 aagagcttca cctaccccaa atacagtctc acaatgccca gcaccgccgc cagtagctat
3421 tccaacgtca ccgtgcccct gaccagcgcc tgggacgtgc ccatctacac cgggcccgat
3481 atcatcctcc cgagtcacac ccccatgtgg ccctccacca gccccacaaa cgccagtaca
3541 acaacataca tcggcatcca cgggacccag tactacctgt ggccctggta ctactacttc
3601 cccaagaaga ggaagaggat cccatacttc ttcgccgacg ggttcgtcgc cgcatgagcc
3661 cgggacccag ctttcttgta caaagtggtt cgatctagaa tggctagtgg atcccccggg
3721 ctgcaggaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa
3781 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
3841 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
3901 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
3961 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
4021 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
4081 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
4141 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
4201 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
4261 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
4321 ctttgggccg cctccccgca tcgataccgt cggcccgttt aaacccgctg atcagcctcg
4381 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
4441 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
4501 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
4561 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
4621 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
4681 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
4741 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
4801 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
4861 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
4921 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
4981 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
5041 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
5101 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcaga
5161 attctatcaa atatttaaag aaaaaaaaat tgtatcaact ttctacaatc tctttcagaa
5221 gacagaagca gagggaatac ttcctaaatc attcaactag gccagcatta ccttaatacc
5281 ggaactagaa aatgacatta caagaaaaga aaacaacaga ccaatatctc tcatgaacaa
5341 agatacaaac attttcaaca aaatattagc aaaaagaatc caagaatgta tcaaaaaata
5401 tacaccacaa ccaagtagaa tttattccag atatgtaagg gtggttcaac gtttgaaaat
5461 caattaacgt aatttgtccc atcaacaggt taaagaagaa aatcacatgg tcatattgat
5521 agacacagaa aaagcatttg acaaaattta acacccattc atgatgcaat ctctcagtaa
5581 actaggaata gaggaaaact tcctcagctt gaatgtacct tcctctcaat tttgctatga
5641 acctgaaact cctcttaaaa aataaagttt ttcatttaaa aagaaaacaa aaaacatgga
5701 ggagcgttga tgtatctcat tttagaccaa tcagctatgg atagttaggc gacagcacag
5761 atagctgctg tacttctgtt tctggcaatg ttccagacta catttaaaaa atttttaatt
5821 atagacttgt acttaatgtt caagaaaaat atgaaaatgg ctttgccgtg ttaatgctac
5881 tcttttttaa aaaaaactaa agttcaaact ttatttatat ttcattagtt ttttagctac
5941 tgttcttttt ctgttctggg atctcattca gaatgccaca ttacatataa ttctcatgtc
6001 tccttgggtt cctcttagtt ttgacagttc ctcagacttt tcttattttt gatgaccttg
6061 acagttttga ggagtactgg ttagatatag ggtaatggtt tttaaagtat atttgtcatg
6121 atttatactg gggtaagggt ttggggagga agcccatggg gtaaagtact gttctcatca
6181 catcatatca aggttatata ccatcaatat tgccacagat gttacttagc cttttaatat
6241 ttctctaatt tagtgtatat gcaatgatag ttctctgatt tctgagattg agtttctcat
6301 gtgtaatgat tatttagagt ttctctttca tctgttcaaa tttttgtcta gttttatttt
6361 ttactgattt gtaagacttc tttttataat ctgcatatta caattctctt tactggggtg
6421 ttgcaaatat tttctgtcat tctatggcct gacttttctt aatggttttt taattttaaa
6481 aataagtctt aatattcatg caatctaatt aacaatcttt tctttgtggt taggactttg
6541 agtcataaga aatttttctc tacactgaag tcatgatggc atgcttctat attattttct
6601 aaaagattta aagttttgcc ttctccattt agacttataa ttcactggaa tttttttgtg
6661 tgtatggtat gacatatggg ttccctttta ttttttacat ataaatatat ttccctgttt
6721 ttctaaaaaa gaaaaagatc atcattttcc cattgtaaaa tgccatattt ttttcatagg
6781 tcacttacat atatcaatgg gtctgtttct gagctctact ctattttatc agcctcactg
6841 tctatcccca cacatctcat gctttgctct aaatcttgat atttagtgga acattctttc
6901 ccattttgtt ctacaagaat atttttgtta ttgtcttttg ggcttctata tacattttag
6961 aatgaggttg gcaagttaac aaacagcttt tttggggtga acatattgac tacaaattta
7021 tgtggaaaga aagtaccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg
7081 ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg
7141 acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg
7201 tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg
7261 agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga
7321 tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc
7381 acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct
7441 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
7501 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
7561 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
7621 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
7681 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
7741 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
7801 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
7861 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
7921 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
7981 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
8041 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
8101 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
8161 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
8221 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
8281 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
8341 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
8401 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
8461 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
8521 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
8581 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
8641 gcggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
8701 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
8761 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
8821 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
8881 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
8941 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
9001 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
9061 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
9121 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
9181 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
9241 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
9301 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
9361 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
9421 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
9481 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
9541 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
9601 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
9661 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
9721 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
9781 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
9841 gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct
9901 cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt
9961 ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga
10021 caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc
10081 cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
10141 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
10201 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
10261 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
10321 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
10381 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
10441 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
10501 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
10561 tgggagtttg ttttggaacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
10621 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct
10681 ccctatcagt gatagagatc tccctatcag tgatagagat cgtcgacgag ctcgtttagt
10741 gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg
10801 ggaccgatcc agcc
//
