| 质粒名称: | pLVX-EF1alpha-SARS-CoV-2-nsp2-2xStrep-IRES-Puro |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 141368 |
| 存储实验室: | Nevan Krogan |
|
载体骨架基本信息 |
|
| 载体名称: | pLVX-EF1alpha-IRES-Puro |
| 载体出品公司: | Takara Bio |
| 空载体大小: | 8825 bp |
| 完整质粒大小: | 14489 bp |
| 载体类型: | 哺乳细胞表达, Lentiviral |
| 筛选标记: | Puromycin |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 30 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | SARS-CoV-2-nsp2 |
| 物种来源: | SARS-CoV-2 |
| 基因突变: | human codon optimized |
| 启动子: | EF1alpha |
| 标签/融合蛋白: | 2xStrep (C terminal on insert) |
| 克隆方法: | 未知 |
|
文献引用方法 |
|
| 材料和方法部分: | pLVX-EF1alpha-SARS-CoV-2-nsp2-2xStrep-IRES-Puro was a gift from Nevan Krogan (Addgene plasmid # 141368 ; http://n2t.net/addgene:141368 ; RRID:Addgene_141368) |
| 参考文献部分: | A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Gordon DE, Jang GM, Bouhaddou M, Xu J, Obernier K, White KM, O'Meara MJ, Rezelj VV, Guo JZ, Swaney DL, Tummino TA, Huttenhain R, Kaake RM, Richards AL, Tutuncuoglu B, Foussard H, Batra J, Haas K, Modak M, Kim M, Haas P, Polacco BJ, Braberg H, Fabius JM, Eckhardt M, Soucheray M, Bennett MJ, Cakir M, McGregor MJ, Li Q, Meyer B, Roesch F, Vallet T, Mac Kain A, Miorin L, Moreno E, Naing ZZC, Zhou Y, Peng S, Shi Y, Zhang Z, Shen W, Kirby IT, Melnyk JE, Chorba JS, Lou K, Dai SA, Barrio-Hernandez I, Memon D, Hernandez-Armenta C, Lyu J, Mathy CJP, Perica T, Pilla KB, Ganesan SJ, Saltzberg DJ, Rakesh R, Liu X, Rosenthal SB, Calviello L, Venkataramanan S, Liboy-Lugo J, Lin Y, Huang XP, Liu Y, Wankowicz SA, Bohn M, Safari M, Ugur FS, Koh C, Savar NS, Tran QD, Shengjuler D, Fletcher SJ, O'Neal MC, Cai Y, Chang JCJ, Broadhurst DJ, Klippsten S, Sharp PP, Wenzell NA, Kuzuoglu-Ozturk D, Wang HY, Trenker R, Young JM, Cavero DA, Hiatt J, Roth TL, Rathore U, Subramanian A, Noack J, Hubert M, Stroud RM, Frankel AD, Rosenberg OS, Verba KA, Agard DA, Ott M, Emerman M, Jura N, von Zastrow M, Verdin E, Ashworth A, Schwartz O, d'Enfert C, Mukherjee S, Jacobson M, Malik HS, Fujimori DG, Ideker T, Craik CS, Floor SN, Fraser JS, Gross JD, Sali A, Roth BL, Ruggero D, Taunton J, Kortemme T, Beltrao P, Vignuzzi M, Garcia-Sastre A, Shokat KM, Shoichet BK, Krogan NJ. Nature. 2020 Jul;583(7816):459-468. doi: 10.1038/s41586-020-2286-9. Epub 2020 Apr 30. 10.1038/s41586-020-2286-9 PubMed 32353859 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLVX-EF1alpha-SARS-CoV-2-nsp2-2xStrep-IRES-Puro 10830 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Lentiviral expression of SARS-CoV-2 protein; transient expression
and generate lentivirus.
ACCESSION .
VERSION .
KEYWORDS pLVX-EF1alpha-SARS-CoV-2-nsp2-2xStrep-IRES-Puro
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10830)
AUTHORS Gordon DE, et al.
TITLE A SARS-CoV-2-Human Protein-Protein Interaction Map Reveals Drug
Targets and Potential Drug Repurposing
JOURNAL BioRxiv March 2020
REFERENCE 2 (bases 1 to 10830)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..10830
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 1..634
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 681..806
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1303..1536
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1721..1765
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2028..2143
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1 (lacking the first T)"
promoter 2338..3519
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 2568..3510
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 3467..3487
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
regulatory 3544..3553
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 5485..5508
/codon_start=1
/product="peptide that binds Strep-Tactin(R), an engineered
form of streptavidin"
/label=Strep-Tag II
/translation="WSHPQFEK"
CDS 5545..5568
/codon_start=1
/product="peptide that binds Strep-Tactin(R), an engineered
form of streptavidin"
/label=Strep-Tag II
/translation="WSHPQFEK"
misc_feature 5580..6153
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(5747..5764)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 5974..5993
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 6186..6785
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(6186..6205)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 6682..6702
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
misc_feature 6799..7387
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(6852..6872)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(7270..7281)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 7594..8227
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
primer_bind complement(8355..8371)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8355..8371)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(8368..8390)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 8379..8395
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(8403..8433)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8448..8469
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(8586..8603)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8757..9345)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8837..8856)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(9516..10376)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 10139..10158
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(10377..10481)
/gene="bla"
/label=AmpR promoter
polyA_signal 10529..10663
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(10566..10585)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 10620..10639
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg
2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc
2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct
2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatctatttc cggtgaattc
3541 gccgccacca tggcctacac gcgatatgtt gacaacaact tttgtggacc tgacggctac
3601 cctcttgaat gtattaagga tcttctggct cgcgctggaa aagccagttg tacactgtct
3661 gagcagctcg actttataga tacaaagcgg ggggtctatt gttgccgaga gcatgaacat
3721 gagatcgcat ggtatacgga gcgctctgaa aaaagctatg aattgcagac accctttgaa
3781 attaagttgg cgaaaaagtt cgatacattc aatggtgagt gccccaactt tgtctttccc
3841 cttaacagta tcattaaaac aatccagccc cgggtggaaa agaagaaatt ggatggattc
3901 atgggcagga tcagaagtgt ttacccagtg gcatccccta atgaatgcaa tcagatgtgc
3961 ctgtccacgc tgatgaaatg cgaccactgc ggtgaaacgt cttggcaaac gggggacttt
4021 gtaaaagcaa cgtgcgagtt ttgcggaact gagaacttga caaaggaggg agcgactaca
4081 tgtggatacc tgcctcagaa cgcggtagtg aaaatatact gtcctgcctg tcacaattct
4141 gaagttgggc ctgaacatag cttggccgaa taccataacg aaagtggatt gaagacaatt
4201 cttcgcaaag gaggccggac tatcgcgttc gggggctgcg tgtttagcta tgtaggatgt
4261 cataataaat gcgcatattg ggttccacga gctagcgcaa acataggctg caaccacaca
4321 ggcgtagttg gtgagggatc agaaggtctt aatgataact tgctcgaaat attgcaaaag
4381 gaaaaagtca acataaatat tgtaggagat tttaaattga atgaagagat tgcgataatc
4441 cttgcgtcat tttccgcaag tacgagtgcg ttcgttgaga ctgtaaaggg gctcgactat
4501 aaagcgttta agcaaatagt agagagctgc ggaaacttta aagttacgaa gggaaaagcc
4561 aaaaaaggtg catggaacat tggcgagcag aagagtatac tgtcccccct gtacgccttc
4621 gcgtcagaag cagctcgagt agttagaagt atattctcac gaacgctcga gacggcgcag
4681 aatagtgtgc gcgtgctcca gaaggctgct attacgatcc tcgacggcat atcccagtat
4741 tctcttcgac tgattgatgc tatgatgttc acctccgact tggcgacgaa caatctcgta
4801 gttatggctt atatcaccgg cggcgttgta caattgacgt ctcagtggct gacgaacatc
4861 ttcggaaccg tgtacgagaa gctcaaacca gttcttgatt ggcttgaaga aaagtttaag
4921 gaaggcgtcg agtttctccg ggatgggtgg gaaattgtta agtttatctc cacctgcgcg
4981 tgtgaaattg tgggagggca aattgtcacc tgcgccaagg aaattaaaga gtcagttcag
5041 acgtttttta aacttgtgaa caaattcctt gctctctgtg cggatagtat aatcatcggt
5101 ggggcgaagt tgaaagcact caatcttggt gaaactttcg tgacccacag caaaggcctg
5161 tataggaagt gtgttaaaag cagggaggag accggtcttc ttatgccgct gaaggcgccc
5221 aaagagataa tttttcttga gggggaaact cttccgaccg aagtgctcac ggaagaagta
5281 gttctgaaaa caggggacct tcaaccactg gagcaaccga cctctgaggc agttgaagcg
5341 ccgctcgtcg gaactcctgt atgtattaac ggcctcatgc tgttggaaat taaggatacg
5401 gagaagtact gtgcactggc ccccaacatg atggtcacga acaacacctt tacccttaag
5461 ggcggcctcg aaggcggcgg gggatggagc catccacaat tcgagaaagg cggtggttca
5521 ggaggaggta gcgggggtgg atcatggtca catccgcagt ttgaaaagta aggatcccgc
5581 ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt
5641 gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg
5701 aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga
5761 atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa
5821 acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc
5881 tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac
5941 gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag
6001 gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc
6061 acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg
6121 gacgtggttt tcctttgaaa aacacgatga taagcttgcc acaacccaca aggagacgac
6181 cttccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg
6241 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc
6301 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
6361 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
6421 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
6481 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
6541 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
6601 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
6661 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
6721 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
6781 cctagacgcg tctggaacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
6841 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
6901 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
6961 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
7021 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
7081 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
7141 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
7201 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
7261 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
7321 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
7381 tccccgcctg gaattaattc tgcagtcgag acctagaaaa acatggagca atcacaagta
7441 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg
7501 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag
7561 atcttagcca ctttttaaaa gaaaagaggg gactggaagg gctaattcac tcccaacgaa
7621 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga
7681 actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag
7741 taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac
7801 accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg
7861 acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact
7921 gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc
7981 tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc
8041 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
8101 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
8161 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
8221 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
8281 atatcagaga gtgagaggcc ttgacattgc tagcgtttac cgtcgacctc tagctagagc
8341 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
8401 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
8461 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
8521 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
8581 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
8641 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
8701 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
8761 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
8821 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
8881 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
8941 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
9001 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
9061 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
9121 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
9181 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
9241 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
9301 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
9361 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
9421 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
9481 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
9541 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
9601 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
9661 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
9721 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
9781 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
9841 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
9901 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
9961 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
10021 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
10081 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
10141 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
10201 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
10261 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
10321 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
10381 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
10441 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
10501 ccacctgacg tcgacggatc gggagatcaa cttgtttatt gcagcttata atggttacaa
10561 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
10621 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc
10681 taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg
10741 gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat
10801 ttgttaaata tgtactacaa acttagtagt
//
