| 质粒名称: | pHR-scFv-GCN4-sfGFP-GB1-dWPRE |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 60907 |
| 存储实验室: | Ron Vale |
|
载体骨架基本信息 |
|
| 载体名称: | pHR |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | scFv-GCN4 |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pHR-scFv-GCN4-sfGFP-GB1-dWPRE was a gift from Ron Vale (Addgene plasmid # 60907 ; http://n2t.net/addgene:60907 ; RRID:Addgene_60907) |
| 参考文献部分: | A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging. Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD. Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039. 10.1016/j.cell.2014.09.039 PubMed 25307933 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pHR-scFv-GCN4-sfGFP-GB1-dWPRE 10059 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION The plasmid encodes an antibody that binds to the GCN4 peptide from
the SunTag system, and is fused to sfGFP for imaging.
ACCESSION .
VERSION .
KEYWORDS pHR-scFv-GCN4-sfGFP-GB1-dWPRE
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10059)
AUTHORS Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD
TITLE A Protein-Tagging System for Signal Amplification in Gene Expression
and Fluorescence Imaging.
JOURNAL Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi:
10.1016/j.cell.2014.09.039.
PUBMED 25307933
REFERENCE 2 (bases 1 to 10059)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10059
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter complement(16..34)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(17..34)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(324..343)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind complement(557..576)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 676..698
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(736..754)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 822..926
/gene="bla"
/label=AmpR promoter
CDS 927..1787
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(1145..1164)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 1958..2546
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 2447..2466
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 2700..2717
/label=L4440
/note="L4440 vector, forward primer"
primer_bind complement(2787..2807)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 2792..3121
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 2972..3107
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3034..3053
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
intron 4255..4320
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 4450..4470
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 4895..5029
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4932..4951)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 4986..5005
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
LTR 5197..5830
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 5877..6002
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 6499..6732
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 6917..6961
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 7246..7363
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 7516..7923
/label=SFFV promoter
/note="spleen focus-forming virus long terminal repeat
(LTR) promoter"
regulatory 7957..7966
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 8704..8730
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 8821..9531
/codon_start=1
/product="GFP variant that folds robustly even when fused
to poorly folded proteins (Pedelacq et al., 2006)"
/label=superfolder GFP
/translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
VTAAGITHGMDELYK"
primer_bind complement(8848..8876)
/label=GFP-R
/note="GFP, reverse primer. Does NOT anneal to EGFP"
primer_bind 9471..9491
/label=GFP-F
/note="GFP, forward primer. Does NOT anneal to EGFP"
CDS 9553..9714
/codon_start=1
/product="B1 domain of Streptococcal protein G "
/label=GB1
/note="effective as a solubilizing fusion partner (Cheng
and Patel, 2004)"
/translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
ATKTFTVTE"
LTR 9817..10050
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
ORIGIN
1 attaattccg tgtattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat
61 gtattaattg tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg tagcatctgg
121 cttactgaag cagaccctat catctctctc gtaaactgcc gtcagagtcg gtttggttgg
181 acgaaccttc tgagtttctg gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat
241 cagcagggtc atcgctagcc agatcctcta cgccggacgc atcgtggccg gcatcaccgg
301 cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc
361 tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc
421 cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa
481 cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg
541 aatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
601 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
661 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
721 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
781 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
841 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
901 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
961 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
1021 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
1081 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
1141 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
1201 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
1261 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
1321 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
1381 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
1441 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
1501 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
1561 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
1621 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
1681 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
1741 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
1801 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
1861 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
1921 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
1981 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
2041 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
2101 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
2161 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
2221 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
2281 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
2341 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
2401 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
2461 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
2521 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
2581 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
2641 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
2701 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
2761 gttggccgat tcattaatgc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
2821 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
2881 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
2941 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
3001 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
3061 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
3121 aaagcttgga cacaagacag gcttgcgaga tatgtttgag aataccactt tatcccgcgt
3181 cagggagagg cagtgcgtaa aaagacgcgg actcatgtga aatactggtt tttagtgcgc
3241 cagatctcta taatctcgcg caacctattt tcccctcgaa cactttttaa gccgtagata
3301 aacaggctgg gacacttcac atgagcgaaa aatacatcgt cacctgggac atgttgcaga
3361 tccatgcacg taaactcgca agccgactga tgccttctga acaatggaaa ggcattattg
3421 ccgtaagccg tggcggtctg taccgggtgc gttactggcg cgtgaactgg gtattcgtca
3481 tgtcgatacc gtttgtattt ccagctacga tcacgacaac cagcgcgagc ttaaagtgct
3541 gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt gatgacctgg tggataccgg
3601 tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc
3661 aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc aagatacctg
3721 gattgaacag ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc
3781 ttttcaacgc ctggcactgc cgggcgttgt tctttttaac ttcaggcggg ttacaatagt
3841 ttccagtaag tattctggag gctgcatcca tgacacaggc aaacctgagc gaaaccctgt
3901 tcaaaccccg ctttaaacat cctgaaacct cgacgctagt ccgccgcttt aatcacggcg
3961 cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg tatcgcatga
4021 ttaaccgtct gatgtggatc tggcgcggca ttgacccacg cgaaatcctc gacgtccagg
4081 cacgtattgt gatgagcgat gccgaacgta ccgacgatga tttatacgat acggtgattg
4141 gctaccgtgg cggcaactgg atttatgagt gggccccgga tctttgtgaa ggaaccttac
4201 ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat
4261 ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag
4321 attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac
4381 ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat
4441 tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg
4501 ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac
4561 accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc
4621 tttataagta ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg
4681 catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt
4741 tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag
4801 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
4861 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
4921 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
4981 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac
5041 tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta
5101 cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa
5161 ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt cactcccaaa
5221 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc
5281 agaactacac accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc
5341 tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt
5401 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt
5461 ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga
5521 actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg
5581 gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt
5641 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
5701 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
5761 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
5821 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
5881 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
5941 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
6001 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
6061 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
6121 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
6181 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
6241 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
6301 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag
6361 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
6421 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
6481 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
6541 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
6601 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
6661 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
6721 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
6781 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
6841 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
6901 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
6961 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
7021 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
7081 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
7141 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
7201 gaacggatct cgacggtatc gccaaatggc agtattcatc cacaatttta aaagaaaagg
7261 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
7321 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
7381 cagcagagat ccagtttgga tcgataagct tgatatcgaa ttcctgcagc cccgataaaa
7441 taaaagattt tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt
7501 ggcaagctag ctgcagtaac gccattttgc aaggcatgga aaaataccaa accaagaata
7561 gagaagttca gatcaagggc gggtacatga aaatagctaa cgttgggcca aacaggatat
7621 ctgcggtgag cagtttcggc cccggcccgg ggccaagaac agatggtcac cgcagtttcg
7681 gccccggccc gaggccaaga acagatggtc cccagatatg gcccaaccct cagcagtttc
7741 ttaagaccca tcagatgttt ccaggctccc ccaaggacct gaaatgaccc tgcgccttat
7801 ttgaattaac caatcagcct gcttctcgct tctgttcgcg cgcttctgct tcccgagctc
7861 tataaaagag ctcacaaccc ctcactcggc gcgccagtcc tccgacagac tgagtcgccc
7921 gggggggatc tggagctctc gagaattctc acgcgtgcca ccatgggccc cgacatcgtg
7981 atgacccaga gccccagcag cctgagcgcc agcgtgggcg accgcgtgac catcacctgc
8041 cgcagcagca ccggcgccgt gaccaccagc aactacgcca gctgggtgca ggagaagccc
8101 ggcaagctgt tcaagggcct gatcggcggc accaacaacc gcgcccccgg cgtgcccagc
8161 cgcttcagcg gcagcctgat cggcgacaag gccaccctga ccatcagcag cctgcagccc
8221 gaggacttcg ccacctactt ctgcgccctg tggtacagca accactgggt gttcggccag
8281 ggcaccaagg tggagctgaa gcgcggcggc ggcggcagcg gcggcggcgg cagcggcggc
8341 ggcggcagca gcggcggcgg cagcgaggtg aagctgctgg agagcggcgg cggcctggtg
8401 cagcccggcg gcagcctgaa gctgagctgc gccgtgagcg gcttcagcct gaccgactac
8461 ggcgtgaact gggtgcgcca ggcccccggc cgcggcctgg agtggatcgg cgtgatctgg
8521 ggcgacggca tcaccgacta caacagcgcc ctgaaggacc gcttcatcat cagcaaggac
8581 aacggcaaga acaccgtgta cctgcagatg agcaaggtgc gcagcgacga caccgccctg
8641 tactactgcg tgaccggcct gttcgactac tggggccagg gcaccctggt gaccgtgagc
8701 agctacccat acgatgttcc agattacgct ggtggaggcg gaggttctgg gggaggaggt
8761 agtggcggtg gtggttcagg aggcggcgga agcttggatc caggtggagg tggaagcggt
8821 agcaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat
8881 gttaatgggc acaaattttc tgtccgtgga gagggtgaag gtgatgctac aaacggaaaa
8941 ctcaccctta aatttatttg cactactgga aaactacctg ttccgtggcc aacacttgtc
9001 actactctga cctatggtgt tcaatgcttt tcccgttatc cggatcacat gaaacggcat
9061 gactttttca agagtgccat gcccgaaggt tatgtacagg aacgcactat atctttcaaa
9121 gatgacggga cctacaagac gcgtgctgaa gtcaagtttg aaggtgatac ccttgttaat
9181 cgtatcgagt taaagggtat tgattttaaa gaagatggaa acattcttgg acacaaactc
9241 gagtacaact ttaactcaca caatgtatac atcacggcag acaaacaaaa gaatggaatc
9301 aaagctaact tcaaaattcg ccacaacgtt gaagatggtt ccgttcaact agcagaccat
9361 tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg
9421 tcgacacaat ctgtcctttc gaaagatccc aacgaaaagc gtgaccacat ggtccttctt
9481 gagtttgtaa ctgctgctgg gattacacat ggcatggatg agctctacaa aggtggaggt
9541 cggaccgaag agtacaagct tatcctgaac ggtaaaaccc tgaaaggtga aaccaccacc
9601 gaagctgttg acgctgctac cgcggaaaaa gttttcaaac agtacgctaa cgacaacggt
9661 gttgacggtg aatggaccta cgacgacgct accaaaacct tcacggtaac cgaataaact
9721 agttaagcgg ccgcgactct agagtcgacc ctttaagacc aatgacttac aaggcagctg
9781 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
9841 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
9901 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
9961 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
10021 ccttttagtc agtgtggaaa atctctagca gcatctaga
//
