pLenti6-tdTomato-V5-DEST

价格:3000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: --
载体名称: pLenti6-tdTomato-V5-DEST
质粒类型: 慢病毒载体;gateway载体;荧光报告载体
高拷贝/低拷贝: 低拷贝
克隆方法: gateway
启动子: CMV
载体大小: 10113 bp
5' 测序引物及序列: CMV fwd :CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄青霉素、氯霉素
筛选标记: blasticidin
克隆菌株: 阳性筛选用DB3.1 ,阴性筛选用OmniMAX2-T1, 重组慢病毒载体的复制用Stbl3感受态细胞,以维持质粒的稳定性。
宿主细胞(系): 哺乳动物细胞
备注:
                      
 
产品目录号: --
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

pLenti6-tdTomato-V5-DEST载体图谱



载体简介

载体序列

LOCUS       Exported               10113 bp ds-DNA     circular SYN 11-AUG-2016
KEYWORDS    pLenti6-tdTomato-V5-DEST
FEATURES             Location/Qualifiers
     source          1..10113
                     /organism="Lentiviral vector pLenti6-tdTomato-V5-Blast"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent University, 
                     Belgium) accession number LMBP 8401."
                     /db_xref="taxon:1783462"
     misc_feature    1..16
                     /note="cPPT"
                     /note="HIV-1 cPPT"
     regulatory      17..251
                     /regulatory_class="terminator"
                     /note="(dU3)3' LTR"
                     /note="RSV/HIV-1 dU3/3' LTR"
     regulatory      323..397
                     /regulatory_class="terminator"
                     /note="SV40 polyA"
                     /note="SV40 polyA early"
     regulatory      complement(323..397)
                     /regulatory_class="terminator"
                     /note="SV40 polyA late"
     rep_origin      533..608
                     /note="SV40 ori"
     regulatory      complement(642..658)
                     /regulatory_class="promoter"
                     /note="T7"
                     /note="T7 promoter"
     rep_origin      826..1281
                     /note="f1 ori"
                     /note="f1 origin"
     gene            1412..2272
                     /gene="bla"
                     /note="Amp"
     CDS             1412..2272
                     /codon_start=1
                     /transl_table=11
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="bla"
                     /note="ampicillin resistance"
                     /db_xref="GI:982281016"
                     /protein_id="CVH74126.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2813..3215
                     /note="pMB1"
                     /note="pMB1 ori"
     regulatory      3307..3390
                     /regulatory_class="promoter"
                     /note="lac promoter"
     regulatory      3454..3470
                     /regulatory_class="promoter"
                     /note="T3 promoter"
     regulatory      3579..3907
                     /regulatory_class="promoter"
                     /note="5' LTR"
                     /note="RSV/HIV-1 5' LTR"
     misc_feature    4018..4062
                     /note="psi"
                     /note="HIV-1 psi packaging signal-psi"
     misc_feature    4572..4805
                     /note="RRE"
                     /note="HIV-1 Rev response element"
     regulatory      5304..5820
                     /regulatory_class="promoter"
                     /note="CMV promoter"
                     /note="hCMV-IE promoter and enhancer"
     regulatory      5937..6061
                     /regulatory_class="attenuator"
                     /note="attR1"
                     /note="gateway recombination site attR1"
     gene            6170..6829
                     /gene="cat"
                     /note="cat"
     CDS             6170..6829
                     /codon_start=1
                     /transl_table=11
                     /gene="cat"
                     /product="chloramphenicol acetyl transferase"
                     /note="CAT"
                     /note="chloramphenicol resistance"
                     /db_xref="GI:982281017"
                     /protein_id="CVH74127.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     gene            7171..7476
                     /gene="ccdB"
                     /note="ccdB"
     CDS             7171..7476
                     /codon_start=1
                     /transl_table=11
                     /gene="ccdB"
                     /product="ccdB"
                     /note="ccdB"
                     /note="B gene of the control of cell death locus of the 
                     Escherichia coli F plasmid"
                     /db_xref="GI:982281018"
                     /protein_id="CVH74128.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     regulatory      7517..7641
                     /regulatory_class="attenuator"
                     /note="attR2"
                     /note="gateway recombination site attR2"
     gene            7673..9172
                     /gene="tdTomato"
                     /note="tdTomato"
     CDS             7673..9172
                     /codon_start=1
                     /transl_table=11
                     /gene="tdTomato"
                     /product="tdTomato"
                     /note="tdTomato"
                     /note="Orange-red fluorescent protein tandem dimer with V5 
                     epitope tag"
                     /db_xref="GI:982281019"
                     /protein_id="CVH74130.1"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYKEGPRFEGKPIPNPLLGLDSTRTG"
     regulatory      9193..9475
                     /regulatory_class="promoter"
                     /note="SV40 promoter"
                     /note="SV40 early promoter"
     rep_origin      9432..9507
                     /note="SV40 ori"
     regulatory      9580..9645
                     /regulatory_class="promoter"
                     /note="EM7"
                     /note="synthetic EM7 promoter"
     gene            9646..10044
                     /gene="bsr"
                     /note="bsr"
     CDS             9646..10044
                     /codon_start=1
                     /transl_table=11
                     /gene="bsr"
                     /product="blasticidin S-resistance"
                     /note="blasticidin"
                     /db_xref="GI:982281020"
                     /protein_id="CVH74131.1"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
ORIGIN
        1 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
       61 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
      121 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
      181 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
      241 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
      301 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
      361 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
      421 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
      481 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
      541 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
      601 ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg
      661 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
      721 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
      781 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg
      841 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
      901 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
      961 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     1021 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     1081 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     1141 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     1201 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     1261 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat
     1321 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     1381 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     1441 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     1501 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     1561 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     1621 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     1681 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     1741 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     1801 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     1861 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     1921 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     1981 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     2041 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     2101 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     2161 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     2221 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     2281 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     2341 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     2401 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     2461 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     2521 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     2581 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     2641 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     2701 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     2761 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     2821 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     2881 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     2941 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     3001 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     3061 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     3121 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     3181 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     3241 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     3301 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     3361 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     3421 aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa
     3481 gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta
     3541 acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg
     3601 gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt
     3661 ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac
     3721 ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     3781 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     3841 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     3901 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
     3961 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
     4021 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
     4081 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
     4141 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
     4201 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
     4261 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
     4321 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
     4381 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga
     4441 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
     4501 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
     4561 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
     4621 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
     4681 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
     4741 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
     4801 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
     4861 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
     4921 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
     4981 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
     5041 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
     5101 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
     5161 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa
     5221 ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga
     5281 tctcgacggt atcgataagc ttgggagttc cgcgttacat aacttacggt aaatggcccg
     5341 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     5401 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     5461 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     5521 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     5581 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
     5641 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
     5701 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
     5761 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
     5821 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga
     5881 agacaccgac tctagaggat ccactagtcc agtgtggtgg aattctgcag atatcaacaa
     5941 gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt
     6001 agattttgca taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat
     6061 ggcggccgca ttaggcaccc caggctttac actttatgct tccggctcgt ataatgtgtg
     6121 gattttgagt taggatccgg cgagattttc aggagctaag gaagctaaaa tggagaaaaa
     6181 aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc
     6241 atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt
     6301 tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc
     6361 ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat
     6421 atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc
     6481 gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt
     6541 ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt
     6601 cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga
     6661 caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct
     6721 gatgccgctg gcgattcagg ttcatcatgc cgtctgtgat ggcttccatg tcggcagaat
     6781 gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaaa gatctggatc
     6841 cggcttacta aaagccagat aacagtatgc gtatttgcgc gctgattttt gcggtataag
     6901 aatatatact gatatgtata cccgaagtat gtcaaaaaga ggtgtgctat gaagcagcgt
     6961 attacagtga cagttgacag cgacagctat cagttgctca aggcatatat gatgtcaata
     7021 tctccggtct ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt gccgaacgct
     7081 ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg gtttattgaa atgaacggct
     7141 cttttgctga cgagaacagg gactggtgaa atgcagttta aggtttacac ctataaaaga
     7201 gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac gcccgggcga
     7261 cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt
     7321 tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt
     7381 gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc
     7441 aaaaacgcca ttaacctgat gttctgggga atataaatgt caggctccgt tatacacagc
     7501 cagtctgcag gtcgaccata gtgactggat atgttgtgtt ttacagtatt atgtagtctg
     7561 ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc
     7621 agctttcttg tacaaagtgg ttgatatcca gcacagtggc ggccgctcga gtatggtgag
     7681 caagggcgag gaggtcatca aagagttcat gcgcttcaag gtgcgcatgg agggctccat
     7741 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
     7801 gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc
     7861 cccccagttc atgtacggct ccaaggcgta cgtgaagcac cccgccgaca tccccgatta
     7921 caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     7981 cggtctggtg accgtgaccc aggactcctc cctgcaggac ggcacgctga tctacaaggt
     8041 gaagatgcgc ggcaccaact tcccccccga cggccccgta atgcagaaga agaccatggg
     8101 ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca
     8161 ccaggccctg aagctgaagg acggcggcca ctacctggtg gagttcaaga ccatctacat
     8221 ggccaagaag cccgtgcaac tgcccggcta ctactacgtg gacaccaagc tggacatcac
     8281 ctcccacaac gaggactaca ccatcgtgga acagtacgag cgctccgagg gccgccacca
     8341 cctgttcctg gggcatggca ccggcagcac cggcagcggc agctccggca ccgcctcctc
     8401 cgaggacaac aacatggccg tcatcaaaga gttcatgcgc ttcaaggtgc gcatggaggg
     8461 ctccatgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     8521 cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgggacat
     8581 cctgtccccc cagttcatgt acggctccaa ggcgtacgtg aagcaccccg ccgacatccc
     8641 cgattacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     8701 ggacggcggt ctggtgaccg tgacccagga ctcctccctg caggacggca cgctgatcta
     8761 caaggtgaag atgcgcggca ccaacttccc ccccgacggc cccgtaatgc agaagaagac
     8821 catgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga
     8881 gatccaccag gccctgaagc tgaaggacgg cggccactac ctggtggagt tcaagaccat
     8941 ctacatggcc aagaagcccg tgcaactgcc cggctactac tacgtggaca ccaagctgga
     9001 catcacctcc cacaacgagg actacaccat cgtggaacag tacgagcgct ccgagggccg
     9061 ccaccacctg ttcctgtacg gcatggacga gctgtacaag gagggcccgc ggttcgaagg
     9121 taagcctatc cctaaccctc tcctcggtct cgattctacg cgtaccggtt agtaatgagt
     9181 ttggaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     9241 ggcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     9301 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     9361 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     9421 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     9481 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     9541 gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca
     9601 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc caagcctttg
     9661 tctcaagaag aatccaccct cattgaaaga gcaacggcta caatcaacag catccccatc
     9721 tctgaagact acagcgtcgc cagcgcagct ctctctagcg acggccgcat cttcactggt
     9781 gtcaatgtat atcattttac tgggggacct tgtgcagaac tcgtggtgct gggcactgct
     9841 gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga tcggaaatga gaacaggggc
     9901 atcttgagcc cctgcggacg gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa
     9961 gccatagtga aggacagtga tggacagccg acggcagttg ggattcgtga attgctgccc
    10021 tctggttatg tgtgggaggg ctaagcacaa ttcgagctcg gtacctttaa gaccaatgac
    10081 ttacaaggca gctgtagatc ttagccactt ttt
//

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