pHage2-EF1a-TERT-IRES-Puro

LOCUS       pHage2-EF1a-TERT-IRES-Puro       11633 bp    DNA     circular SYN 14-APR-2025
DEFINITION  expresses TERT in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11633)
  AUTHORS   Laprade H, Querido E, Smith MJ, Guerit D, Crimmins H, Conomos D, 
            Pourret E, Chartrand P, Sfeir A
  TITLE     Single-Molecule Imaging of Telomerase RNA Reveals a 
            Recruitment-Retention Model for Telomere Elongation.
  JOURNAL   Mol Cell. 2020 Jul 2;79(1):115-126.e6. doi: 
            10.1016/j.molcel.2020.05.005. Epub 2020 Jun 3.
  PUBMED    32497497
REFERENCE   2  (bases 1 to 11633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.05.005"; journalName: "Mol Cell"; date: 
            "2020-07-2- 2"; volume: "79"; issue: "1"; pages: "115-126.e6"
COMMENT     Sequence Label: pHage2-EF1a-TERT-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..11633
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3526..6924
                     /codon_start=1
                     /gene="TERT"
                     /product="human telomerase reverse transcriptase, the 
                     catalytic subunit of telomerase"
                     /label=hTERT
                     /note="prevents telomere shortening and allows 
                     postsenescent cells to proliferate beyond crisis (Counter 
                     et al., 1998); also known as EST2"
                     /translation="MPRAPRCRAVRSLLRSHYREVLPLATFVRRLGPQGWRLVQRGDPA
                     AFRALVAQCLVCVPWDARPPPAAPSFRQVSCLKELVARVLQRLCERGAKNVLAFGFALL
                     DGARGGPPEAFTTSVRSYLPNTVTDALRGSGAWGLLLRRVGDDVLVHLLARCALFVLVA
                     PSCAYQVCGPPLYQLGAATQARPPPHASGPRRRLGCERAWNHSVREAGVPLGLPAPGAR
                     RRGGSASRSLPLPKRPRRGAAPEPERTPVGQGSWAHPGRTRGPSDRGFCVVSPARPAEE
                     ATSLEGALSGTRHSHPSVGRQHHAGPPSTSRPPRPWDTPCPPVYAETKHFLYSSGDKEQ
                     LRPSFLLSSLRPSLTGARRLVETIFLGSRPWMPGTPRRLPRLPQRYWQMRPLFLELLGN
                     HAQCPYGVLLKTHCPLRAAVTPAAGVCAREKPQGSVAAPEEEDTDPRRLVQLLRQHSSP
                     WQVYGFVRACLRRLVPPGLWGSRHNERRFLRNTKKFISLGKHAKLSLQELTWKMSVRGC
                     AWLRRSPGVGCVPAAEHRLREEILAKFLHWLMSVYVVELLRSFFYVTETTFQKNRLFFY
                     RKSVWSKLQSIGIRQHLKRVQLRELSEAEVRQHREARPALLTSRLRFIPKPDGLRPIVN
                     MDYVVGARTFRREKRAERLTSRVKALFSVLNYERARRPGLLGASVLGLDDIHRAWRTFV
                     LRVRAQDPPPELYFVKVDVTGAYDTIPQDRLTEVIASIIKPQNTYCVRRYAVVQKAAHG
                     HVRKAFKSHVSTLTDLQPYMRQFVAHLQETSPLRDAVVIEQSSSLNEASSGLFDVFLRF
                     MCHHAVRIRGKSYVQCQGIPQGSILSTLLCSLCYGDMENKLFAGIRRDGLLLRLVDDFL
                     LVTPHLTHAKTFLRTLVRGVPEYGCVVNLRKTVVNFPVEDEALGGTAFVQMPAHGLFPW
                     CGLLLDTRTLEVQSDYSSYARTSIRASLTFNRGFKAGRNMRRKLFGVLRLKCHSLFLDL
                     QVNSLQTVCTNIYKILLLQAYRFHACVLQLPFHQQVWKNPTFFLRVISDTASLCYSILK
                     AKNAGMSLGAKGAAGPLPSEAVQWLCHQAFLLKLTRHRVTYVPLLGSLRTAQTQLSRKL
                     PGTTLTALEAAANPALPSDFKTILD"
     misc_feature    6975..7436
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7031..7048)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7258..7277
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7441..8040
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7441..7460)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7937..7957
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8075..8663
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8128..8148)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8546..8557)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8811..8991
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9038..9056)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9124..9228
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9229..9297,9298..10089)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9447..9466)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10260..10848
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11002..11019
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11259..11280
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11295..11300,11301..11318,11319..11325)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11333..11349
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11338..11360
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11357..11373
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11357..11373
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11423..11442
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagcttcagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccgc ttaattaagt
     3421 ttaaacggat cccgccggcg gctagcgtta acacgcgttg tacattttac gtaggaattc
     3481 gtcctgctgc gcacgtggga agccctggcc ccggccaccc ccgcgatgcc gcgcgctccc
     3541 cgctgccgag ccgtgcgctc cctgctgcgc agccactacc gcgaggtgct gccgctggcc
     3601 acgttcgtgc ggcgcctggg gccccagggc tggcggctgg tgcagcgcgg ggacccggcg
     3661 gctttccgcg cgctggtggc ccagtgcctg gtgtgcgtgc cctgggacgc acggccgccc
     3721 cccgccgccc cctccttccg ccaggtgtcc tgcctgaagg agctggtggc ccgagtgctg
     3781 cagaggctgt gcgagcgcgg cgcgaagaac gtgctggcct tcggcttcgc gctgctggac
     3841 ggggcccgcg ggggcccccc cgaggccttc accaccagcg tgcgcagcta cctgcccaac
     3901 acggtgaccg acgcactgcg ggggagcggg gcgtgggggc tgctgctgcg ccgcgtgggc
     3961 gacgacgtgc tggttcacct gctggcacgc tgcgcgctct ttgtgctggt ggctcccagc
     4021 tgcgcctacc aggtgtgcgg gccgccgctg taccagctcg gcgctgccac tcaggcccgg
     4081 cccccgccac acgctagtgg accccgaagg cgtctgggat gcgaacgggc ctggaaccat
     4141 agcgtcaggg aggccggggt ccccctgggc ctgccagccc cgggtgcgag gaggcgcggg
     4201 ggcagtgcca gccgaagtct gccgttgccc aagaggccca ggcgtggcgc tgcccctgag
     4261 ccggagcgga cgcccgttgg gcaggggtcc tgggcccacc cgggcaggac gcgtggaccg
     4321 agtgaccgtg gtttctgtgt ggtgtcacct gccagacccg ccgaagaagc cacctctttg
     4381 gagggtgcgc tctctggcac gcgccactcc cacccatccg tgggccgcca gcaccacgcg
     4441 ggccccccat ccacatcgcg gccaccacgt ccctgggaca cgccttgtcc cccggtgtac
     4501 gccgagacca agcacttcct ctactcctca ggcgacaagg agcagctgcg gccctccttc
     4561 ctactcagct ctctgaggcc cagcctgact ggcgctcgga ggctcgtgga gaccatcttt
     4621 ctgggttcca ggccctggat gccagggact ccccgcaggt tgccccgcct gccccagcgc
     4681 tactggcaaa tgcggcccct gtttctggag ctgcttggga accacgcgca gtgcccctac
     4741 ggggtgctcc tcaagacgca ctgcccgctg cgagctgcgg tcaccccagc agccggtgtc
     4801 tgtgcccggg agaagcccca gggctctgtg gcggcccccg aggaggagga cacagacccc
     4861 cgtcgcctgg tgcagctgct ccgccagcac agcagcccct ggcaggtgta cggcttcgtg
     4921 cgggcctgcc tgcgccggct ggtgccccca ggcctctggg gctccaggca caacgaacgc
     4981 cgcttcctca ggaacaccaa gaagttcatc tccctgggga agcatgccaa gctctcgctg
     5041 caggagctga cgtggaagat gagcgtgcgg ggctgcgctt ggctgcgcag gagcccaggg
     5101 gttggctgtg ttccggccgc agagcaccgt ctgcgtgagg agatcctggc caagttcctg
     5161 cactggctga tgagtgtgta cgtcgtcgag ctgctcaggt ctttctttta tgtcacggag
     5221 accacgtttc aaaagaacag gctctttttc taccggaaga gtgtctggag caagttgcaa
     5281 agcattggaa tcagacagca cttgaagagg gtgcagctgc gggagctgtc ggaagcagag
     5341 gtcaggcagc atcgggaagc caggcccgcc ctgctgacgt ccagactccg cttcatcccc
     5401 aagcctgacg ggctgcggcc gattgtgaac atggactacg tcgtgggagc cagaacgttc
     5461 cgcagagaaa agagggccga gcgtctcacc tcgagggtga aggcactgtt cagcgtgctc
     5521 aactacgagc gggcgcggcg ccccggcctc ctgggcgcct ctgtgctggg cctggacgat
     5581 atccacaggg cctggcgcac cttcgtgctg cgtgtgcggg cccaggaccc gccgcctgag
     5641 ctgtactttg tcaaggtgga tgtgacgggc gcgtacgaca ccatccccca ggacaggctc
     5701 acggaggtca tcgccagcat catcaaaccc cagaacacgt actgcgtgcg tcggtatgcc
     5761 gtggtccaga aggccgccca tgggcacgtc cgcaaggcct tcaagagcca cgtctctacc
     5821 ttgacagacc tccagccgta catgcgacag ttcgtggctc acctgcagga gaccagcccg
     5881 ctgagggatg ccgtcgtcat cgagcagagc tcctccctga atgaggccag cagtggcctc
     5941 ttcgacgtct tcctacgctt catgtgccac cacgccgtgc gcatcagggg caagtcctac
     6001 gtccagtgcc aggggatccc gcagggctcc atcctctcca cgctgctctg cagcctgtgc
     6061 tacggcgaca tggagaacaa gctgtttgcg gggattcggc gggacgggct gctcctgcgt
     6121 ttggtggatg atttcttgtt ggtgacacct cacctcaccc acgcgaaaac cttcctcagg
     6181 accctggtcc gaggtgtccc tgagtatggc tgcgtggtga acttgcggaa gacagtggtg
     6241 aacttccctg tagaagacga ggccctgggt ggcacggctt ttgttcagat gccggcccac
     6301 ggcctattcc cctggtgcgg cctgctgctg gatacccgga ccctggaggt gcagagcgac
     6361 tactccagct atgcccggac ctccatcaga gccagtctca ccttcaaccg cggcttcaag
     6421 gctgggagga acatgcgtcg caaactcttt ggggtcttgc ggctgaagtg tcacagcctg
     6481 tttctggatt tgcaggtgaa cagcctccag acggtgtgca ccaacatcta caagatcctc
     6541 ctgctgcagg cgtacaggtt tcacgcatgt gtgctgcagc tcccatttca tcagcaagtt
     6601 tggaagaacc ccacattttt cctgcgcgtc atctctgaca cggcctccct ctgctactcc
     6661 atcctgaaag ccaagaacgc agggatgtcg ctgggggcca agggcgccgc cggccctctg
     6721 ccctccgagg ccgtgcagtg gctgtgccac caagcattcc tgctcaagct gactcgacac
     6781 cgtgtcacct acgtgccact cctggggtca ctcaggacag cccagacgca gctgagtcgg
     6841 aagctcccgg ggacgacgct gactgccctg gaggccgcag ccaacccggc actgccctca
     6901 gacttcaaga ccatcctgga ctgagtcgac caattccggt tattttccac catattgccg
     6961 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
     7021 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt
     7081 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac
     7141 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca
     7201 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg
     7261 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg
     7321 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaac
     7381 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataatacc
     7441 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     7501 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     7561 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     7621 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     7681 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     7741 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     7801 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     7861 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     7921 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     7981 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
     8041 agtagatgcc gaccgggatc tatcgataga tcctaatcaa cctctggatt acaaaatttg
     8101 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     8161 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     8221 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     8281 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     8341 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     8401 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     8461 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     8521 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     8581 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     8641 ctccctttgg gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg
     8701 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     8761 gaagacaaga tcacctgcag gacaggcgcg ccctgctttt tgcttgtact gggtctctct
     8821 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     8881 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     8941 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc acccgggcga
     9001 ttaaggaaag ggctagatca ttcttgaaga cgaaagggcc tcgtgatacg cctattttta
     9061 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     9121 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     9181 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     9241 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     9301 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     9361 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     9421 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc
     9481 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     9541 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     9601 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     9661 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     9721 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     9781 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     9841 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     9901 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     9961 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
    10021 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
    10081 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
    10141 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
    10201 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
    10261 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
    10321 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
    10381 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
    10441 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
    10501 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
    10561 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
    10621 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
    10681 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
    10741 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
    10801 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
    10861 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
    10921 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
    10981 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
    11041 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagcaagct catggctgac
    11101 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
    11161 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctccccgtg gcacgacagg
    11221 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
    11281 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
    11341 ggataacaat ttcacacagg aaacagctat gacatgatta cgaatttcac aaataaagca
    11401 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
    11461 tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac cccataccct
    11521 attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta
    11581 ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt agt
//