| 质粒名称: | pXDC61 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 21841 |
| 存储实验室: | Howard Shuman |
|
载体骨架基本信息 |
|
| 载体名称: | pMMB207C |
| 载体抗性: | 氯霉素 |
| 空载体大小: | 9024bp |
| 载体类型: | 细菌表达 |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | blaM |
| 物种来源: | 大肠杆菌 |
| 插入大小: | 845 bp |
| 克隆方法: | 限制性酶 |
| 5' 克隆位点: | Ecori（未销毁） |
| 3' 克隆位点: |
印地语（未销毁）
|
| 5' 测序引物 | GAGCTGTTGACAATTAATCATCGG |
|
文献引用方法 |
|
| 材料和方法部分: | pXDC61 was a gift from Howard Shuman (Addgene plasmid # 21841 ; http://n2t.net/addgene:21841 ; RRID:Addgene_21841) |
| 参考文献部分: |
Chemical genetics reveals bacterial and host cell functions critical for type IV effector translocation by Legionella pneumophila. Charpentier X, Gabay JE, Reyes M, Zhu JW, Weiss A, Shuman HA. PLoS Pathog. 2009 Jul . 5(7):e1000501. 10.1371/journal.ppat.1000501 PubMed 19578436 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pXDC61 9869 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pXDC61
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9869)
AUTHORS Charpentier X, Gabay JE, Reyes M, Zhu JW, Weiss A, Shuman HA
TITLE Chemical genetics reveals bacterial and host cell functions critical
for type IV effector translocation by Legionella pneumophila.
JOURNAL PLoS Pathog. 2009 Jul . 5(7):e1000501.
PUBMED 19578436
REFERENCE 2 (bases 1 to 9869)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9869
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1..29
/label=tac promoter
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
protein_bind 37..53
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 42..64
/label=M13/pUC Reverse
/note="In lacZ gene"
CDS 101..886
/codon_start=1
/gene="bla(M)"
/product="beta-lactamase lacking the signal sequence"
/label=bla(M)
/note="allows cytosolic expression of beta-lactamase"
/translation="PETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMST
FKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMS
DNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATT
LRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAA
LGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
primer_bind complement(247..266)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
primer_bind complement(953..970)
/label=pBAD Reverse
/note="For vectors with E. coli araBAD promoter, reverse
primer"
terminator 1123..1209
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 1301..1328
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
promoter 1347..1438
/gene="bla"
/label=AmpR promoter
promoter 1889..1991
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
CDS 1992..2651
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
primer_bind complement(2058..2077)
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
rep_origin 2983..3377
/label=RSF1010 oriV
/note="replication origin of the broad-host-range plasmid
RSF1010 from Salmonella enterica serovar Typhimurium;
requires the RSF1010 RepA/B/C proteins for replication"
CDS 5045..6016
/codon_start=1
/gene="repB"
/product="replication protein B of the broad-host-range
plasmid RSF1010 from Salmonella enterica serovar
Typhimurium"
/label=RSF1010 RepB
/translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDAPTGQMMNREWSAAEVL
QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
EYRSEMAGLVKRFGHDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
CDS 6527..7366
/codon_start=1
/gene="repA"
/product="replication protein A of the broad-host-range
plasmid RSF1010 from Salmonella enterica serovar
Typhimurium"
/label=RSF1010 RepA
/translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
CDS 7353..8204
/codon_start=1
/gene="repC"
/product="replication protein C of the broad-host-range
plasmid RSF1010 from Salmonella enterica serovar
Typhimurium"
/label=RSF1010 RepC
/translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
TAECHVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
TGKASIDTLCGYVWPSEASGSTMRKRRKRVREALPELVALGWTVTEFAAGKYDITRPKA
AG"
primer_bind 9524..9543
/label=LacI-R
/note="LacI, reverse primer"
promoter complement(9563..9640)
/gene="lacI (mutant)"
/label=lacIq promoter
/note="In the lacIq allele, a single base change in the
promoter boosts expression of the lacI gene about 10-fold."
ORIGIN
1 ttgacaatta atcatcggct cgtataatgt gtggaattgt gagcggataa caatttcaca
61 caggaaacag aattcagaag gagatataca tatggctcac ccagaaacgc tggtgaaagt
121 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
181 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
241 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
301 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
361 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
421 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
481 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
541 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
601 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
661 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
721 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
781 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
841 aaatagacag atcgctgaga taggtgcctc actgattaag cattggggaa gcggtacccg
901 gggatcctct agataagctt ggctgttttg gcggatgaga gaagattttc agcctgatac
961 agattaaatc agaacgcaga agcggtctga taaaacagaa tttgcctggc ggcagtagcg
1021 cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc gccgatggta
1081 gtgtggggtc tccccatgcg agagtaggga actgccaggc atcaaataaa acgaaaggct
1141 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
1201 aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg agggtggcgg
1261 gcaggacgcc cgccataaac tgccaggcat caaattaagc agaaggccat cctgacggat
1321 ggcctttttg cgtttctaca aactcttttg tttatttttc taaatacatt caaatatgta
1381 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
1441 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
1501 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
1561 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
1621 agaacgtgat gtccggcggt gcttttgccg ttacgcacca ccccgtcagt agctgaacag
1681 gagggacagc tgatagaaac agaagccact ggagcaagca cctcaaaaac accatcatac
1741 actaaatcag taagttggca gcatcacccg acgcactttg cgccgaataa atacctgtga
1801 cggaagatca cttcgcagaa taaataaatc ctggtgtccc tgttgatacc gggaagccct
1861 gggccaactt ttggcgaaaa tgagacgttg atcggcacgt aagaggttcc aactttcacc
1921 ataatgaaat aagatcacta ccgggcgtat tttttgagtt atcgagattt tcaggagcta
1981 aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc
2041 atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg
2101 ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc
2161 cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaatta cgtatggcaa
2221 tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg
2281 agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc
2341 tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag
2401 ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg
2461 atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt
2521 atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg
2581 atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg
2641 gcggggcgta atttttttaa ggcagttatt gtcttcaaat tcccgttgca catagcccgg
2701 caattccttt ccctgctctg ccataagcgc agcgaatgcc gggtaatact cgtcaacgat
2761 ctgatagaga agggtttgct cgggtcggtg gctctggtaa cgaccagtat cccgatcccg
2821 gctggccgtc ctggccgcca catgaggcat gttccgcgtc cttgcaatac tgtgtttaca
2881 tacagtctat cgcttagcgg aaagttcttt taccctcagc cgaaatgcct gccgttgcta
2941 gacattgcca gccagtgccc gtcactcccg tactaactgt cacgaacccc tgcaataact
3001 gtcacgcccc cctgcaataa ctgtcacgaa cccctgcaat aactgtcacg cccccaaacc
3061 tgcaaaccca gcaggggcgg gggctggcgg ggtgttggaa aaatccatcc atgattatct
3121 aagaataatc cactaggcgc ggttatcagc gcccttgtgg ggcgctgctg cccttgccca
3181 atatgcccgg ccagaggccg gatagctggt ctattcgctg cgctaggcta cacaccgccc
3241 caccgctgcg cggcaggggg aaaggcgggc aaagcccgct aaaccccaca ccaaaccccg
3301 cagaaatacg ctggagcgct tttagccgct ttagcggcct ttccccctac ccgaagggtg
3361 ggggcgcgtg tgcagccccg cagggcctgt ctcggtcgat cattcagccc ggctcatcct
3421 tctggcgtgg cggcagaccg aacaaggcgc ggtcgtggtc gcgttcaagg tacgcatcca
3481 ttgccgccat gagccgatcc tccggccact cgctgctgtt caccttggcc aaaatcatgg
3541 cccccaccag caccttgcgc cttgtttcgt tcttgcgctc ttgctgctgt tcccttgccc
3601 gctcccgctg aatttcggca ttgattcgcg ctcgttgttc ttcgagcttg gccagccgat
3661 ccgccgcctt gttgctcccc ttaaccatct tgacacccca ttgttaatgt gctgtctcgt
3721 aggctatcat ggaggcacag cggcggcaat cccgacccta ctttgtaggg gagggcgcac
3781 ttaccggttt ctcttcgaga aactggccta acggccaccc ttcgggcggt gcgctctccg
3841 agggccattg catggagccg aaaagcaaaa gcaacagcga ggcagcatgg cgatttatca
3901 ccttacggcg aaaaccggca gcaggtcggg cggccaatcg gccagggcca aggccgacta
3961 catccagcgc gaaggcaagt atgcccgcga catggatgaa gtcttgcacg ccgaatccgg
4021 gcacatgccg gagttcgtcg agcggcccgc cgactactgg gatgctgccg acctgtatga
4081 acgcgccaat gggcggctgt tcaaggaggt cgaatttgcc ctgccggtcg agctgaccct
4141 cgaccagcag aaggcgctgg cgtccgagtt cgcccagcac ctgaccggtg ccgagcgcct
4201 gccgtatacg ctggccatcc atgccggtgg cggcgagaac ccgcactgcc acctgatgat
4261 ctccgagcgg atcaatgacg gcatcgagcg gcccgccgct cagtggttca agcggtacaa
4321 cggcaagacc ccggagaagg gcggggcaca gaagaccgaa gcgctcaagc ccaaggcatg
4381 gcttgagcag acccgcgagg catgggccga ccatgccaac cgggcattag agcgggctgg
4441 ccacgacgcc cgcattgacc acagaacact tgaggcgcag ggcatcgagc gcctgcccgg
4501 tgttcacctg gggccgaacg tggtggagat ggaaggccgg ggcatccgca ccgaccgggc
4561 agacgtggcc ctgaacatcg acaccgccaa cgcccagatc atcgacttac aggaataccg
4621 ggaggcaata gaccatgaac gcaatcgaca gagtgaagaa atccagaggc atcaacgagt
4681 tagcggagca gatcgaaccg ctggcccaga gcatggcgac actggccgac gaagcccggc
4741 aggtcatgag ccagaccaag caggccagcg aggcgcaggc ggcggagtgg ctgaaagccc
4801 agcgccagac aggggcggca tgggtggagc tggccaaaga gttgcgggag gtagccgccg
4861 aggtgagcag cgccgcgcag agcgcccgga gcgcgtcgcg ggggtggcac tggaagctat
4921 ggctaaccgt gatgctggct tccatgatgc ctacggtggt gctgctgatc gcatcgttgc
4981 tcttgctcga cctgacgcca ctgacaaccg aggacggctc gatctggctg cgcttggtgg
5041 cccgatgaag aacgacagga ctttgcaggc cataggccga cagctcaagg ccatgggctg
5101 tgagcgcttc gatatcggcg tcagggacgc acccaccggc cagatgatga accgggaatg
5161 gtcagccgcc gaagtgctcc agaacacgcc atggctcaag cggatgaatg cccagggcaa
5221 tgacgtgtat atcaggcccg ccgagcagga gcggcatggt ctggtgctgg tggacgacct
5281 cagcgagttt gacctggatg acatgaaagc cgagggccgg gagcctgccc tggtagtgga
5341 aaccagcccg aagaactatc aggcatgggt caaggtggcc gacgccgcag gcggtgaact
5401 tcgggggcag attgcccgga cgctggccag cgagtacgac gccgacccgg ccagcgccga
5461 cagccgccac tatggccgct tggcgggctt caccaaccgc aaggacaagc acaccacccg
5521 cgccggttat cagccgtggg tgctgctgcg tgaatccaag ggcaagaccg ccaccgctgg
5581 cccggcgctg gtgcagcagg ctggccagca gatcgagcag gcccagcggc agcaggagaa
5641 ggcccgcagg ctggccagcc tcgaactgcc cgagcggcag cttagccgcc accggcgcac
5701 ggcgctggac gagtaccgca gcgagatggc cgggctggtc aagcgcttcg gtcatgacct
5761 cagcaagtgc gactttatcg ccgcgcagaa gctggccagc cggggccgca gtgccgagga
5821 aatcggcaag gccatggccg aggccagccc agcgctggca gagcgcaagc ccggccacga
5881 agcggattac atcgagcgca ccgtcagcaa ggtcatgggt ctgcccagcg tccagcttgc
5941 gcgggccgag ctggcacggg caccggcacc ccgccagcga ggcatggaca ggggcgggcc
6001 agatttcagc atgtagtgct tgcgttggta ctcacgcctg ttatactatg agtactcacg
6061 cacagaaggg ggttttatgg aatacgaaaa aagcgcttca gggtcggtct acctgatcaa
6121 aagtgacaag ggctattggt tgcccggtgg ctttggttat acgtcaaaca aggccgaggc
6181 tggccgcttt tcagtcgctg atatggccag ccttaacctt gacggctgca ccttgtcctt
6241 gttccgcgaa gacaagcctt tcggccccgg caagtttctc ggtgactgat atgaaagacc
6301 aaaaggacaa gcagaccggc gacctgctgg ccagccctga cgctgtacgc caagcgcgat
6361 atgccgagcg catgaaggcc aaagggatgc gtcagcgcaa gttctggctg accgacgacg
6421 aatacgaggc gctgcgcgag tgcctggaag aactcagagc ggcgcagggc gggggtagtg
6481 accccgccag cgcctaacca ccaactgcct gcaaaggagg caatcaatgg ctacccataa
6541 gcctatcaat attctggagg cgttcgcagc agcgccgcca ccgctggact acgttttgcc
6601 caacatggtg gccggtacgg tcggggcgct ggtgtcgccc ggtggtgccg gtaaatccat
6661 gctggccctg caactggccg cacagattgc aggcgggccg gatctgctgg aggtgggcga
6721 actgcccacc ggcccggtga tctacctgcc cgccgaagac ccgcccaccg ccattcatca
6781 ccgcctgcac gcccttgggg cgcacctcag cgccgaggaa cggcaagccg tggctgacgg
6841 cctgctgatc cagccgctga tcggcagcct gcccaacatc atggccccgg agtggttcga
6901 cggcctcaag cgcgccgccg agggccgccg cctgatggtg ctggacacgc tgcgccggtt
6961 ccacatcgag gaagaaaacg ccagcggccc catggcccag gtcatcggtc gcatggaggc
7021 catcgccgcc gataccgggt gctctatcgt gttcctgcac catgccagca agggcgcggc
7081 catgatgggc gcaggcgacc agcagcaggc cagccggggc agctcggtac tggtcgataa
7141 catccgctgg cagtcctacc tgtcgagcat gaccagcgcc gaggccgagg aatggggtgt
7201 ggacgacgac cagcgccggt tcttcgtccg cttcggtgtg agcaaggcca actatggcgc
7261 accgttcgct gatcggtggt tcaggcggca tgacggcggg gtgctcaagc ccgccgtgct
7321 ggagaggcag cgcaagagca agggggtgcc ccgtggtgaa gcctaagaac aagcacagcc
7381 tcagccacgt ccggcacgac ccggcgcact gtctggcccc cggcctgttc cgtgccctca
7441 agcggggcga gcgcaagcgc agcaagctgg acgtgacgta tgactacggc gacggcaagc
7501 ggatcgagtt cagcggcccg gagccgctgg gcgctgatga tctgcgcatc ctgcaagggc
7561 tggtggccat ggctgggcct aatggcctag tgcttggccc ggaacccaag accgaaggcg
7621 gacggcagct ccggctgttc ctggaaccca agtgggaggc cgtcaccgct gaatgccatg
7681 tggtcaaagg tagctatcgg gcgctggcaa aggaaatcgg ggcagaggtc gatagtggtg
7741 gggcgctcaa gcacatacag gactgcatcg agcgcctttg gaaggtatcc atcatcgccc
7801 agaatggccg caagcggcag gggtttcggc tgctgtcgga gtacgccagc gacgaggcgg
7861 acgggcgcct gtacgtggcc ctgaacccct tgatcgcgca ggccgtcatg ggtggcggcc
7921 agcatgtgcg catcagcatg gacgaggtgc gggcgctgga cagcgaaacc gcccgcctgc
7981 tgcaccagcg gctgtgtggc tggatcgacc ccggcaaaac cggcaaggct tccatagata
8041 ccttgtgcgg ctatgtctgg ccgtcagagg ccagtggttc gaccatgcgc aagcgccgca
8101 agcgggtgcg cgaggcgttg ccggagctgg tcgcgctggg ctggacggta accgagttcg
8161 cggcgggcaa gtacgacatc acccggccca aggcggcagg ctgacccccc ccactctatt
8221 gtaaacaaga catttttatc ttttatattc aatggcttat tttcctgcta attggtaata
8281 ccatgaaaaa taccatgctc agaaaaggct taacaatatt ttgaaaaatt gcctactgag
8341 cgctgccgca cagctccata ggccgctttc ctggctttgc ttccagatgt atgctcttct
8401 gctcccgaac gccagcaaga cgtagcccag cgcgtcggcc agcttgcaat tcgcgctaac
8461 ttacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
8521 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgccagggtg
8581 gtttttcttt tcaccagtga gacgggcaac agctgattgc ccttcaccgc ctggccctga
8641 gagagttgca gcaagcggtc cacgtggttt gccccagcag gcgaaaatcc tgtttgatgg
8701 tggttaacgg cgggatataa catgagctgt cttcggtatc gtcgtatccc actaccgaga
8761 tatccgcacc aacgcgcagc ccggactcgg taatggcgcg cattgcgccc agcgccatct
8821 gatcgttggc aaccagcatc gcagtgggaa cgatgccctc attcagcatt tgcatggttt
8881 gttgaaaacc ggacatggca ctccagtcgc cttcccgttc cgctatcggc tgaatttgat
8941 tgcgagtgag atatttatgc cagccagcca gacgcagacg cgccgagaca gaacttaatg
9001 ggcccgctaa cagcgcgatt tgctggtgac ccaatgcgac cagatgctcc acgcccagtc
9061 gcgtaccgtc ttcatgggag aaaataatac tgttgatggg tgtctggtca gagacatcaa
9121 gaaataacgc cggaacatta gtgcaggcag cttccacagc aatggcatcc tggtcatcca
9181 gcggatagtt aatgatcagc ccactgacgc gttgcgcgag aagattgtgc accgccgctt
9241 tacaggcttc gacgccgctt cgttctacca tcgacaccac cacgctggca cccagttgat
9301 cggcgcgaga tttaatcgcc gcgacaattt gcgacggcgc gtgcagggcc agactggagg
9361 tggcaacgcc aatcagcaac gactgtttgc ccgccagttg ttgtgccacg cggttgggaa
9421 tgtaattcag ctccgccatc gccgcttcca ctttttcccg cgttttcgca gaaacgtggc
9481 tggcctggtt caccacgcgg gaaacggtct gataagagac accggcatac tctgcgacat
9541 cgtataacgt tactggtttc acattcacca ccctgaattg actctcttcc gggcgctatc
9601 atgccatacc gcgaaaggtt ttgcaccatt cgatggtgtc aacgtaaatg ccgcttcgcc
9661 ttcgcgcgcg aattgcaagc tgatccgggc ttatcgactg cacggtgcac caatgcttct
9721 ggcgtcaggc agccatcgga agctgtggta tggctgtgca ggtcgtaaat cactgcataa
9781 ttcgtgtcgc tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg acatcataac
9841 ggttctggca aatattctga aatgagctg
//
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