pRS405

价格:1500元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Stratagene(Agilent)
载体名称: pRS405
质粒类型: 酿酒酵母载体;酵母着丝粒质粒;穿梭载体
克隆方法: 多克隆位点,限制性内切酶
高拷贝/低拷贝: 单拷贝
启动子: --
载体大小: 5507 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄青霉素Ampicillin
筛选标记: LEU2
克隆宿主: --
备注: 含a beta-gal报告基因;宿主菌株YPH499,YPH500,YPH501
产品目录号: --
稳定性: --
组成型/诱导型: 诱导型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pRS405载体图谱



载体简介

载体序列

pRS405, 5507 bp                               version 013001

NOTE: The following sequence has been verified for accuracy
at the junctions. The remainder of the sequence has been 
obtained from existing data. 

   1  TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG  
  51  GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG  
 101  TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG  
 151  CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATCGA CTACGTCGTA  
 201  AGGCCGTTTC TGACAGAGTA AAATTCTTGA GGGAACTTTC ACCATTATGG  
 251  GAAATGCTTC AAGAAGGTAT TGACTTAAAC TCCATCAAAT GGTCAGGTCA  
 301  TTGAGTGTTT TTTATTTGTT GTATTTTTTT TTTTTTAGAG AAAATCCTCC  
 351  AATATCAAAT TAGGAATCGT AGTTTCATGA TTTTCTGTTA CACCTAACTT  
 401  TTTGTGTGGT GCCCTCCTCC TTGTCAATAT TAATGTTAAA GTGCAATTCT  
 451  TTTTCCTTAT CACGTTGAGC CATTAGTATC AATTTGCTTA CCTGTATTCC  
 501  TTTACTATCC TCCTTTTTCT CCTTCTTGAT AAATGTATGT AGATTGCGTA  
 551  TATAGTTTCG TCTACCCTAT GAACATATTC CATTTTGTAA TTTCGTGTCG  
 601  TTTCTATTAT GAATTTCATT TATAAAGTTT ATGTACAAAT ATCATAAAAA  
 651  AAGAGAATCT TTTTAAGCAA GGATTTTCTT AACTTCTTCG GCGACAGCAT  
 701  CACCGACTTC GGTGGTACTG TTGGAACCAC CTAAATCACC AGTTCTGATA  
 751  CCTGCATCCA AAACCTTTTT AACTGCATCT TCAATGGCCT TACCTTCTTC  
 801  AGGCAAGTTC AATGACAATT TCAACATCAT TGCAGCAGAC AAGATAGTGG  
 851  CGATAGGGTC AACCTTATTC TTTGGCAAAT CTGGAGCAGA ACCGTGGCAT  
 901  GGTTCGTACA AACCAAATGC GGTGTTCTTG TCTGGCAAAG AGGCCAAGGA  
 951  CGCAGATGGC AACAAACCCA AGGAACCTGG GATAACGGAG GCTTCATCGG  
1001  AGATGATATC ACCAAACATG TTGCTGGTGA TTATAATACC ATTTAGGTGG  
1051  GTTGGGTTCT TAACTAGGAT CATGGCGGCA GAATCAATCA ATTGATGTTG  
1101  AACCTTCAAT GTAGGGAATT CGTTCTTGAT GGTTTCCTCC ACAGTTTTTC  
1151  TCCATAATCT TGAAGAGGCC AAAAGATTAG CTTTATCCAA GGACCAAATA  
1201  GGCAATGGTG GCTCATGTTG TAGGGCCATG AAAGCGGCCA TTCTTGTGAT  
1251  TCTTTGCACT TCTGGAACGG TGTATTGTTC ACTATCCCAA GCGACACCAT  
1301  CACCATCGTC TTCCTTTCTC TTACCAAAGT AAATACCTCC CACTAATTCT  
1351  CTGACAACAA CGAAGTCAGT ACCTTTAGCA AATTGTGGCT TGATTGGAGA  
1401  TAAGTCTAAA AGAGAGTCGG ATGCAAAGTT ACATGGTCTT AAGTTGGCGT  
1451  ACAATTGAAG TTCTTTACGG ATTTTTAGTA AACCTTGTTC AGGTCTAACA  
1501  CTACCGGTAC CCCATTTAGG ACCAGCCACA GCACCTAACA AAACGGCATC  
1551  AACCTTCTTG GAGGCTTCCA GCGCCTCATC TGGAAGTGGG ACACCTGTAG  
1601  CATCGATAGC AGCACCACCA ATTAAATGAT TTTCGAAATC GAACTTGACA  
1651  TTGGAACGAA CATCAGAAAT AGCTTTAAGA ACCTTAATGG CTTCGGCTGT  
1701  GATTTCTTGA CCAACGTGGT CACCTGGCAA AACGACGATC TTCTTAGGGG  
1751  CAGACATAGG GGCAGACATT AGAATGGTAT ATCCTTGAAA TATATATATA  
1801  TATTGCTGAA ATGTAAAAGG TAAGAAAAGT TAGAAAGTAA GACGATTGCT  
1851  AACCACCTAT TGGAAAAAAC AATAGGTCCT TAAATAATAT TGTCAACTTC  
1901  AAGTATTGTG ATGCAAGCAT TTAGTCATGA ACGCTTCTCT ATTCTATATG  
1951  AAAAGCCGGT TCCGGCCTCT CACCTTTCCT TTTTCTCCCA ATTTTTCAGT  
2001  TGAAAAAGGT ATATGCGTCA GGCGACCTCT GAAATTAACA AAAAATTTCC  
2051  AGTCATCGAA TTTGATTCTG TGCGATAGCG CCCCTGTGTG TTCTCGTTAT  
2101  GTTGAGGAAA AAAATAATGG TTGCTAAGAG ATTCGAACTC TTGCATCTTA  
2151  CGATACCTGA GTATTCCCAC AGTTAACTGC GGTCAAGATA TTTCTTGAAT  
2201  CAGGCGCCTT AGACCGCTCG GCCAAACAAC CAATTACTTG TTGAGAAATA  
2251  GAGTATAATT ATCCTATAAA TATAACGTTT TTGAACACAC ATGAACAAGG  
2301  AAGTACAGGA CAATTGATTT TGAAGAGAAT GTGGATTTTG ATGTAATTGT  
2351  TGGGATTCCA TTTTTAATAA GGCAATAATA TTAGGTATGT GGATATACTA  
2401  GAAGTTCTCC TCGACCGTCG ATATGCGGTG TGAAATACCG CACAGATGCG  
2451  TAAGGAGAAA ATACCGCATC AGGAAATTGT AAACGTTAAT ATTTTGTTAA  
2501  AATTCGCGTT AAATTTTTGT TAAATCAGCT CATTTTTTAA CCAATAGGCC  
2551  GAAATCGGCA AAATCCCTTA TAAATCAAAA GAATAGACCG AGATAGGGTT  
2601  GAGTGTTGTT CCAGTTTGGA ACAAGAGTCC ACTATTAAAG AACGTGGACT  
2651  CCAACGTCAA AGGGCGAAAA ACCGTCTATC AGGGCGATGG CCCACTACGT  
2701  GAACCATCAC CCTAATCAAG TTTTTTGGGG TCGAGGTGCC GTAAAGCACT  
2751  AAATCGGAAC CCTAAAGGGA GCCCCCGATT TAGAGCTTGA CGGGGAAAGC  
2801  CGGCGAACGT GGCGAGAAAG GAAGGGAAGA AAGCGAAAGG AGCGGGCGCT  
2851  AGGGCGCTGG CAAGTGTAGC GGTCACGCTG CGCGTAACCA CCACACCCGC  
2901  CGCGCTTAAT GCGCCGCTAC AGGGCGCGTC GCGCCATTCG CCATTCAGGC  
2951  TGCGCAACTG TTGGGAAGGG CGATCGGTGC GGGCCTCTTC GCTATTACGC  
3001  CAGCTGGCGA AAGGGGGATG TGCTGCAAGG CGATTAAGTT GGGTAACGCC  
3051  AGGGTTTTCC CAGTCACGAC GTTGTAAAAC GACGGCCAGT GAGCGCGCGT  
3101  AATACGACTC ACTATAGGGC GAATTGGGTA CCGGGCCCCC CCTCGAGGTC  
3151  GACGGTATCG ATAAGCTTGA TATCGAATTC CTGCAGCCCG GGGGATCCAC  
3201  TAGTTCTAGA GCGGCCGCCA CCGCGGTGGA GCTCCAGCTT TTGTTCCCTT  
3251  TAGTGAGGGT TAATTGCGCG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC  
3301  TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA GGAGCCGGAA  
3351  GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGGTA ACTCACATTA  
3401  ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA  
3451  GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG  
3501  GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG  
3551  CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC  
3601  AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA  
3651  AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC  
3701  CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG  
3751  AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC  
3801  TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC  
3851  TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA  
3901  TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC  
3951  CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG  
4001  TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA  
4051  CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT  
4101  GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT  
4151  CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG  
4201  CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA  
4251  TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG  
4301  GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT  
4351  GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA  
4401  GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC  
4451  CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC  
4501  ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG  
4551  GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA  
4601  CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG  
4651  CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT  
4701  GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT  
4751  GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC  
4801  TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA  
4851  TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA  
4901  AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA  
4951  TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT  
5001  ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT  
5051  TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA  
5101  AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT  
5151  TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA  
5201  TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG  
5251  AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA  
5301  TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT  
5351  TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT  
5401  AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCTAAGAAA  
5451  CCATTATTAT CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC  
5501  TTTCGTC

载体质粒图谱pdf版和相关资料下载

载体应用举例

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