pBABE-neo

价格:1250元

联系方式:I47-825O-882O

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载体基本信息

出品人: Bob Weinberg
载体名称: pBABE-neo
质粒类型: 逆病毒载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: MSCV
载体大小: 5330 bp
5' 测序引物及序列: pBABE 5' primer 5'CTTTATCCAGCCCTCAC3'
3' 测序引物及序列: pBABE 3' primer 5'ACCCTAACTGACACACATTCC3'
载体标签:
载体抗性: 氨苄青霉素
筛选标记: Neomycin
克隆菌株: DH5α、HB101等
宿主细胞(系): 常规哺乳动物细胞系
备注:
推荐使用逆病毒包装载体:pUMVCpCMV-VSV-G
 
产品目录号: #1767
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 逆转录病毒

载体质粒图谱和多克隆位点信息

pBABE-neo载体图谱



pBABE-neo多克隆位点

载体简介

载体序列

LOCUS       pBABE-neo	5330 bp 	DNA	SYN
DEFINITION  pBABE-neo
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..5330
                     /organism="pBABE-neo"
                     /mol_type="other DNA"
     misc_feature    12..479
                     /label="5_LTR2"
     misc_feature    18..479
                     /label="3MoMuLV_LTR"
     misc_feature    549..1350
                     /label="psi_plus_pack"
     misc_feature    1318..1334
                     /label="pBABE_5_primer"
     misc_feature    complement(1408..1428)
                     /label="pBABE_3_primer"
     misc_feature    complement(1414..1629)
                     /label="SV40_enhancer"
     promoter        1426..1694
                     /label="SV40_promoter"
     rep_origin      1593..1670
                     /label="SV40_origin"
     misc_feature    1655..1674
                     /label="SV40pro_F_primer"
     CDS             1752..2564
                     /label="ORF frame 3"
                     /translation="MTEYKPMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFR
                     LSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGE
                     VPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVD
                     QDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRL
                     GVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     gene            1773..2561
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
                     /translation="MTEYKPMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFR
                     LSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGE
                     VPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVD
                     QDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRL
                     GVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     misc_feature    2611..3204
                     /label="3MoMuLV_LTR"
                     /translation="MTEYKPMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFR
                     LSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGE
                     VPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVD
                     QDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRL
                     GVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     misc_feature    2653..3204
                     /label="5_LTR2"
                     /translation="MTEYKPMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFR
                     LSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGE
                     VPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVD
                     QDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRL
                     GVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     gene            complement(4342..5202)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MTEYKPMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFR
                     LSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGE
                     VPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVD
                     QDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRL
                     GVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     CDS             complement(4342..5202)
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(5244..5272)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
   61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
  121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
  181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
  241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
  301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
  361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
  421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
  481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
  541 taagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
  601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
  661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
  721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
  781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
  841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt
  901 ctgactgtgt ttctgtattt gtctgaaaat tagggccaga ctgttaccac tcccttaagt
  961 ttgaccttag atcactggaa agatgtcgag cggctcgctc acaaccagtc ggtagatgtc
 1021 aagaagagac gttgggttac cttctgctct gcagaatggc caacctttaa cgtcggatgg
 1081 ccgcgagacg gcacctttaa ccgagacctc atcacccagg ttaagatcaa ggtcttttca
 1141 cctggcccgc atggacaccc agaccaggtc ccctacatcg tgacctggga agccttggct
 1201 tttgaccccc ctccctgggt caagcccttt gtacacccta agcctccgcc tcctcttctt
 1261 ccatccgcgc cgtctctccc ccttgaacct cctctttcga ccccgcctca atcctccctt
 1321 tatccagccc tcactccttc tctaggcgcc ggccggatcc cagtgtggtg gtacgtagga
 1381 attcgccagc acagtggtcg accctgtgga atgtgtgtca gttagggtgt ggaaagtccc
 1441 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
 1501 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
 1561 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
 1621 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
 1681 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
 1741 aaaagcttac catgaccgag tacaagccca tgattgaaca agatggattg cacgcaggtt
 1801 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
 1861 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
 1921 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
 1981 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
 2041 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
 2101 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
 2161 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
 2221 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
 2281 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
 2341 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
 2401 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
 2461 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
 2521 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagatcga taaaataaaa
 2581 gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa
 2641 gctagcttaa gtaacgccat tttgcaaggc atggaaaaat acataactga gaatagagaa
 2701 gttcagatca aggtcaggaa cagatggaac agctgaatat gggccaaaca ggatatctgt
 2761 ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggaacagctg aatatgggcc
 2821 aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggtcc
 2881 ccagatgcgg tccagccctc agcagtttct agagaaccat cagatgtttc cagggtgccc
 2941 caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt
 3001 ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcgggg
 3061 cgccagtcct ccgattgact gagtcgcccg ggtacccgtg tatccaataa accctcttgc
 3121 agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact
 3181 acccgtcagc gggggtcttt catttccgac ttgtggtctc gctgccttgg gagggtctcc
 3241 tctgagtgat tgactacccg tcagcggggg tcttcacatg cagcatgtat caaaattaat
 3301 ttggtttttt ttcttaagta tttacattaa atggccatag ttgcattaat gaatcggcca
 3361 acgcgcgggg agaggcggtt tgcgtattgg cgctcttccg cttcctcgct cactgactcg
 3421 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
 3481 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
 3541 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
 3601 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
 3661 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
 3721 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
 3781 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
 3841 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
 3901 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
 3961 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
 4021 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
 4081 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
 4141 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
 4201 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
 4261 acctagatcc ttttaaatta aaaatgaagt ttgcggccgc aaatcaatct aaagtatata
 4321 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
 4381 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
 4441 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
 4501 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
 4561 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
 4621 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
 4681 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
 4741 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
 4801 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
 4861 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
 4921 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca
 4981 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
 5041 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
 5101 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
 5161 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
 5221 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
 5281 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
//

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