pIEX/Bac-1

价格:1350元

联系方式:I47-825O-882O

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pIEXBac1载体基本信息

出品公司: Novagen
载体名称: pIEXBac1, pIEXBac-1, pIEX/Bac1, pIEX/Bac-1
质粒类型: 昆虫细胞表达载体;蛋白纯化
启动子: Polyhedrin
表达水平:
克隆方法: 多克隆位点,限制性内切酶
载体大小: 6796 bp
5' 测序引物: --
3' 测序引物: --
载体标签: C-His, N-EK, N-Strep
载体抗性: Ampicillin 氨苄
备注:
拥有hr5增强子和IE1启动子;双标签系统可以采用两种纯化方案。
产品目录号: 71724-3
稳定性: 瞬转 Transient
组成型: 组成型 Constitutive
病毒/非病毒: 杆状病毒

pIEXBac1载体质粒图谱和多克隆位点信息

pIEX/Bac1载体图谱


pIEX-Bac1多克隆位点

pIEXBac1载体简介

The dual-purpose pIEx/Bac™ vectors are designed for cloning and high-level expression of proteins by transiently transfecting Spodoptera-derived insect cells or by generating baculovirus recombinants. Transient transfection and early baculovirus expression is driven by a promoter/enhancer combination that recruits endogenous insect cell transcription machinery, the AcNPV derived hr5 enhancer and ie1 promoter. Late/very late expression in the baculovirus mode is driven by the strong p10 promoter. The pIEx/Bac-1 vector carries an N-terminal Strep•Tag II coding sequence (1) followed by a recognition site for enterokinase.

The multiple cloning region is followed by an optional C-terminal His•Tag® coding sequence. The presence of two “gentle elution" tags at both the N- and C-terminus is ideal for dual purification strategies designed to isolate full-length fusion proteins (2). Unique restriction sites are shown on the circle map.

pIEXBac1载体序列

LOCUS       pIEx/Bac-1   6796 bp    DNA   circular            17-OCT-2006
DEFINITION  pIEx/BAC hr5/ie1/p10 Strep-Tag II/EkLIC/10xHis.
SOURCE      
  ORGANISM 
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com| 
FEATURES             Location/Qualifiers
     misc_recomb     complement(2916..4334)
                     /dnas_title="1629"
                     /vntifkey=86
                     /label=1629
     rep_origin      6271..6726
                     /dnas_title="f1 origin"
                     /vntifkey=33
                     /label=f1 origin
     CDS             complement(5282..6139)
                     /dnas_title="Ap"
                     /vntifkey=4
                     /label=Ap
     rep_origin      complement(4521..4521)
                     /dnas_title="origin"
                     /vntifkey=33
                     /label=origin
     misc_recomb     4..453
                     /dnas_title="lef-2"
                     /vntifkey=86
                     /label=lef-2
     misc_recomb     complement(491..1096)
                     /dnas_title="ORF 603"
                     /vntifkey=86
                     /label=ORF 603
     rep_origin      4354..5113
                     /dnas_title="pUC Ori"
                     /vntifkey=33
                     /label=pUC Ori
     enhancer        1196..1677
                     /dnas_title="hr5"
                     /vntifkey=9
                     /label=hr5
     polyA_signal    2742..2747
                     /dnas_title="PolyA signal"
                     /vntifkey=25
                     /label=PolyA signal
     5'UTR           2229..2278
                     /dnas_title="ie1 5'-UTR"
                     /vntifkey=52
                     /label=ie1 5'-UTR
     promoter        2279..2391
                     /dnas_title="p10 promoter + 5'UTR"
                     /vntifkey=29
                     /label=p10 promoter + 5'UTR
     promoter        1682..2228
                     /dnas_title="ie1 promoter"
                     /vntifkey=29
                     /label=ie1 promoter
     terminator      2565..2871
                     /dnas_title="ie1 Terminator"
                     /vntifkey=43
                     /label=ie1 Terminator
     protein_bind    2433..2447
                     /dnas_title="Ek site"
                     /vntifkey=31
                     /label=Ek site
     protein_bind    2403..2426
                     /dnas_title="Strep-Tag II"
                     /vntifkey=31
                     /label=Strep-Tag II
     misc_binding    2517..2546
                     /dnas_title="10x His•Tag"
                     /vntifkey=20
                     /label=10x His•Tag
BASE COUNT     1824 a   1462 c   1506 g   2004 t
ORIGIN      
          1 GGAACGGCTC CGCCCACTAT TAATGAAATT AAAAATTCCA ATTTTAAAAA ACGCAGCAAG
         61 AGAAACATTT GTATGAAAGA ATGCGTAGAA GGAAAGAAAA ATGTCGTCGA CATGCTGAAC
        121 AACAAGATTA ATATGCCTCC GTGTATAAAA AAAATATTGA ACGATTTGAA AGAAAACAAT
        181 GTACCGCGCG GCGGTATGTA CAGGAAGAGG TTTATACTAA ACTGTTACAT TGCAAACGTG
        241 GTTTCGTGTG CCAAGTGTGA AAACCGATGT TTAATCAAGG CTCTGACGCA TTTCTACAAC
        301 CACGACTCCA AGTGTGTGGG TGAAGTCATG CATCTTTTAA TCAAATCCCA AGATGTGTAT
        361 AAACCACCAA ACTGCCAAAA AATGAAAACT GTCGACAAGC TCTGTCCGTT TGCTGGCAAC
        421 TGCAAGGGTC TCAATCCTAT TTGTAATTAT TGAATAATAA AACAATTATA AATGCTAAAT
        481 TTGTTTTTTA TTAACGATAC AAACCAAACG CAACAAGAAC ATTTGTAGTA TTATCTATAA
        541 TTGAAAACGC GTAGTTATAA TCGCTGAGGT AATATTTAAA ATCATTTTCA AATGATTCAC
        601 AGTTAATTTG CGACAATATA ATTTTATTTT CACATAAACT AGACGCCTTG TCGTCTTCTT
        661 CTTCGTATTC CTTCTCTTTT TCATTTTTCT CTTCATAAAA ATTAACATAG TTATTATCGT
        721 ATCCATATAT GTATCTATCG TATAGAGTAA ATTTTTTGTT GTCATAAATA TATATGTCTT
        781 TTTTAATGGG GTGTATAGTA CCGCTGCGCA TAGTTTTTCT GTAATTTACA ACAGTGCTAT
        841 TTTCTGGTAG TTCTTCGGAG TGTGTTGCTT TAATTATTAA ATTTATATAA TCAATGAATT
        901 TGGGATCGTC GGTTTTGTAC AATATGTTGC CGGCATAGTA CGCAGCTTCT TCTAGTTCAA
        961 TTACACCATT TTTTAGCAGC ACCGGATTAA CATAACTTTC CAAAATGTTG TACGAACCGT
       1021 TAAACAAAAA CAGTTCACCT CCCTTTTCTA TACTATTGTC TGCGAGCAGT TGTTTGTTGT
       1081 TAAAAATAAC AGCCATTGTA ATGAGACGCA CAAACTAATA TCACAAACTG GAAATGTCTA
       1141 TCAATATATA GTTGCTGATC AGATCTGATC ATGGAGATAA TTAAAATGAT AAGGGCGCGT
       1201 AAAACACAAT CAAGTATGAG TCATAAGCTG ATGTCATGTT TTGCACACGG CTCATAACCG
       1261 AACTGGCTTT ACGAGTAGAA TTCTACTTGT AACGCACGAT CAGTGGATGA TGTCATTTGT
       1321 TTTTCAAATC GAGATGATGT CATGTTTTGC ACACGGCTCA TAAACTCGCT TTACGAGTAG
       1381 AATTCTACGT GTAACGCACG ATCGATTGAT GAGTCATTTG TTTTGCAATA TGATATCATA
       1441 CAATATGACT CATTTGTTTT TCAAAACCGA ACTTGATTTA CGGGTAGAAT TCTACTTGTA
       1501 AAGCACAATC AAAAAGATGA TGTCATTTGT TTTTCAAAAC TGAACTCGCT TTACGAGTAG
       1561 AATTCTACGT GTAAAACACA ATCAAGAAAT GATGTCATTT GTTATAAAAA TAAAAGCTGA
       1621 TGTCATGTTT TGCACATGGC TCATAACTAA ACTCGCTTTA CGGGTAGAAT TCTACGCGCG
       1681 TCGATGTCTT TGTGATGCGC GCGACATTTT TGTAGGTTAT TGATAAAATG AACGGATACG
       1741 TTGCCCGACA TTATCATTAA ATCCTTGGCG TAGAATTTGT CGGGTCCATT GTCCGTGTGC
       1801 GCTAGCATGC CCGTAACGGA CCTCGTACTT TTGGCTTCAA AGGTTTTGCG CACAGACAAA
       1861 ATGTGCCACA CTTGCAGCTC TGCATGTGTG CGCGTTACCA CAAATCCCAA CGGCGCAGTG
       1921 TACTTGTTGT ATGCAAATAA ATCTCGATAA AGGCGCGGCG CGCGAATGCA GCTGATCACG
       1981 TACGTCCCTC GTGTTCCGTT CAAGGACGGT GTTATCGACC TCAGATTAAT GTTTATCGGC
       2041 CGACTGTTTT CGTATCCGCT CACCAAACGC GTTTTTGCAT TAACATTGTA TGTCGGCGGA
       2101 TGTTCTATAT CTAATTTGAA TAAATAAACG ATAACCGCGT TGGTTTTAGA GGGCATAATA
       2161 AAAGAAATAT TGTTATCGTG TTCGCCATTA GGGCAGTATA AATTGACGTT CATGTTGGAT
       2221 ATTGTTTCAG TTGCAAGTTG ACACTGGCGG CGACAAGATC GTGAACAACC AAGTGACTCC
       2281 GGGACCTTTA ATTCAACCCA ACACAATATA TTATAGTTAA ATAAGAATTA TTATCAAATC
       2341 ATTTGTATAT TAATTAAAAT ACTATACTGT AAATTACATT TTATTTACAA TCCATGGCAA
       2401 GCTGGAGCCA CCCGCAGTTC GAAAAGGGTG CAGATGACGA CGACAAGGTA CCGGATCCGA
       2461 CGTCGGTCGA CAAGCTTGCG GCCGCAGGAG CTCCGGGCTT CTCCTCAATT TCCGCTCATC
       2521 ACCACCATCA TCACCATCAC CACCACTAAG TGATTAACCT CAGGTTATAC ATATATTTTG
       2581 AATTTAATTA ATTATACATA TATTTTATAT TATTTTTGTC TTTTATTATC GAGGGGCCGT
       2641 TGTTGGTGTG GGGTTTTGCA TAGAAATAAC AATGGGAGTT GGCGACGTTG CTGCGCCAAC
       2701 ACCACCTCCC TTCCCTCCTT TCATCATGTA TCTGTAGATA AAATAAAATA TTAAACCTAA
       2761 AAACAAGACC GCGCCTATCA ACAAAATGAT AGGCATTAAC TTGCCGCTGA CGCTGTCACT
       2821 AACGTTGGAC GATTTGCCGA CTAAACCTTC ATCGCCCAGT AACCAATCTA GGTAGCTGAG
       2881 CGCATGCAAG CTGATCCGGG TTATTAGTAC ATTTATTAAG CGCTAGATTC TGTGCGTTGT
       2941 TGATTTACAG ACAATTGTTG TACGTATTTT AATAATTCAT TAAATTTATA ATCTTTAGGG
       3001 TGGTATGTTA GAGCGAAAAT CAAATGATTT TCAGCGTCTT TATATCTGAA TTTAAATATT
       3061 AAATCCTCAA TAGATTTGTA AAATAGGTTT CGATTAGTTT CAAACAAGGG TTGTTTTTCC
       3121 GAACCGATGG CTGGACTATC TAATGGATTT TCGCTCAACG CCACAAAACT TGCCAAATCT
       3181 TGTAGCAGCA ATCTAGCTTT GTCGATATTC GTTTGTGTTT TGTTTTGTAA TAAAGGTTCG
       3241 ACGTCGTTCA AAATATTATG CGCTTTTGTA TTTCTTTCAT CACTGTCGTT AGTGTACAAT
       3301 TGACTCGACG TAAACACGTT AAATAGAGCT TGGACATATT TAACATCGGG CGTGTTAGCT
       3361 TTATTAGGCC GATTATCGTC GTCGTCCCAA CCCTCGTCGT TAGAAGTTGC TTCCGAAGAC
       3421 GATTTTGCCA TAGCCACACG ACGCCTATTA ATTGTGTCGG CTAACACGTC CGCGATCAAA
       3481 TTTGTAGTTG AGCTTTTTGG AATTATTTCT GATTGCGGGC GTTTTTGGGC GGGTTTCAAT
       3541 CTAACTGTGC CCGATTTTAA TTCAGACAAC ACGTTAGAAA GCGATGGTGC AGGCGGTGGT
       3601 AACATTTCAG ACGGCAAATC TACTAATGGC GGCGGTGGTG GAGCTGATGA TAAATCTACC
       3661 ATCGGTGGAG GCGCAGGCGG GGCTGGCGGC GGAGGCGGAG GCGGAGGTGG TGGCGGTGAT
       3721 GCAGACGGCG GTTTAGGCTC AAATGTCTCT TTAGGCAACA CAGTCGGCAC CTCAACTATT
       3781 GTACTGGTTT CGGGCGCCGT TTTTGGTTTG ACCGGTCTGA GACGAGTGCG ATTTTTTTCG
       3841 TTTCTAATAG CTTCCAACAA TTGTTGTCTG TCGTCTAAAG GTGCAGCGGG TTGAGGTTCC
       3901 GTCGGCATTG GTGGAGCGGG CGGCAATTCA GACATCGATG GTGGTGGTGG TGGTGGAGGC
       3961 GCTGGAATGT TAGGCACGGG AGAAGGTGGT GGCGGCGGTG CCGCCGGTAT AATTTGTTCT
       4021 GGTTTAGTTT GTTCGCGCAC GATTGTGGGC ACCGGCGCAG GCGCCGCTGG CTGCACAACG
       4081 GAAGGTCGTC TGCTTCGAGG CAGCGCTTGG GGTGGTGGCA ATTCAATATT ATAATTGGAA
       4141 TACAAATCGT AAAAATCTGC TATAAGCATT GTAATTTCGC TATCGTTTAC CGTGCCGATA
       4201 TTTAACAACC GCTCAATGTA AGCAATTGTA TTGTAAAGAG ATTGTCTCAA GCTCGGATCG
       4261 ATCCCGCACG CCGATAACAA GCCTTTTCAT TTTTACTACA GCATTGTAGT GGCGAGACAC
       4321 TTCGCTGTCG TCGAGGTTTA AACGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC
       4381 GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG
       4441 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA
       4501 AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC
       4561 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC
       4621 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG
       4681 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT
       4741 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC
       4801 GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC
       4861 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG
       4921 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG
       4981 CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA
       5041 CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG
       5101 GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT
       5161 CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA
       5221 ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT
       5281 ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG
       5341 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA
       5401 GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC
       5461 AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT
       5521 CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG
       5581 TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA
       5641 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG
       5701 TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA
       5761 TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG
       5821 TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT
       5881 CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA
       5941 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA
       6001 GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG
       6061 TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC
       6121 GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT
       6181 ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC
       6241 CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG
       6301 CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC
       6361 CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA
       6421 ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC
       6481 TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT
       6541 TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA
       6601 ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT
       6661 TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT TAACAAAATA TTAACGTTTA
       6721 CAATTTCCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT
       6781 CTTCGCTATT ACGCCA
//

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