| 质粒名称: | DP6 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 140446 |
| 存储实验室: | David Liu |
|
载体骨架基本信息 |
|
| 载体名称: | CloDF13 |
| 载体出品公司: | -- |
| 载体抗性: | 氯霉素 |
| 空载体大小: | 2992 bp |
| 完整质粒大小: | 9639 bp |
| 载体修饰: | -- |
| 载体类型: | 细菌表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | dnaQ926 dam seqA emrR ugi CDA1 gIII |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | DP6 was a gift from David Liu (Addgene plasmid # 140446 ; http://n2t.net/addgene:140446 ; RRID:Addgene_140446) |
| 参考文献部分: | Development of potent in vivo mutagenesis plasmids with broad mutational spectra. Badran AH, Liu DR. Nat Commun. 2015 Oct 7;6:8425. doi: 10.1038/ncomms9425. 10.1038/ncomms9425 PubMed 26443021 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS DP6 8639 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Untargetted bacterial drift.
ACCESSION .
VERSION .
KEYWORDS DP6
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8639)
AUTHORS Badran AH, Liu DR
TITLE Development of potent in vivo mutagenesis plasmids with broad
mutational spectra.
JOURNAL Nat Commun. 2015 Oct 7;6:8425. doi: 10.1038/ncomms9425.
PUBMED 26443021
REFERENCE 2 (bases 1 to 8639)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8639
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 1..879
/codon_start=1
/gene="araC"
/product="L-arabinose regulatory protein"
/label=araC
/translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
EKVNDVAVKLS"
primer_bind complement(929..946)
/label=pBAD Reverse
/note="For vectors with E. coli araBAD promoter, reverse
primer"
terminator 1099..1185
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 1277..1304
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
protein_bind 1468..1486
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
rep_origin 2987..3725
/direction=RIGHT
/label=CloDF13 ori
/note="Plasmids containing the CloDF13 (CDF) origin of
replication can be propagated in E. coli cells that contain
additional plasmids with compatible origins."
CDS 3878..4537
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAKCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
primer_bind complement(3944..3963)
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
terminator 4553..4584
/label=tonB terminator
/note="bidirectional E. coli tonB-P14 transcription
terminator"
CDS complement(4611..5237)
/codon_start=1
/product="cytidine deaminase from the sea lamprey
Petromyzon marinus (Rogozin et al., 2007)"
/label=PmCDA1
/translation="MTDAEYVRIHEKLDIYTFKKQFFNNKKSVSHRCYVLFELKRRGER
RACFWGYAVNKPQSGTERGIHAEIFSIRKVEEYLRDNPGQFTINWYSSWSPCADCAEKI
LEWYNQELRGNGHTLKIWACKLYYEKNARNQIGLWNLRDNGVGLNVMVSEHYQCCRKIF
IQSSHNQLNENRWLEKTLKRAEKRRSELSIMIQVKILHTTKSPAV"
CDS complement(5267..5521)
/codon_start=1
/product="uracil-DNA glycosylase inhibitor from a Bacillus
subtilis bacteriophage (Mol et al., 1995)"
/label=UGI
/translation="MTNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHT
AYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
promoter complement(8329..8613)
/gene="araBAD"
/label=araBAD promoter
/note="promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction (Guzman et al., 1995)"
primer_bind complement(8387..8406)
/label=pBAD Forward
/note="For vectors with E. coli araBAD promoter, forward
primer"
ORIGIN
1 atggctgaag cgcaaaatga tcccctgctg ccgggatact cgtttaatgc ccatctggtg
61 gcgggtttaa cgccgattga ggccaacggt tatctcgatt tttttatcga ccgaccgctg
121 ggaatgaaag gttatattct caatctcacc attcgcggtc agggggtggt gaaaaatcag
181 ggacgagaat ttgtttgccg accgggtgat attttgctgt tcccgccagg agagattcat
241 cactacggtc gtcatccgga ggctcgcgaa tggtatcacc agtgggttta ctttcgtccg
301 cgcgcctact ggcatgaatg gcttaactgg ccgtcaatat ttgccaatac ggggttcttt
361 cgcccggatg aagcgcacca gccgcatttc agcgacctgt ttgggcaaat cattaacgcc
421 gggcaagggg aagggcgcta ttcggagctg ctggcgataa atctgcttga gcaattgtta
481 ctgcggcgca tggaagcgat taacgagtcg ctccatccac cgatggataa tcgggtacgc
541 gaggcttgtc agtacatcag cgatcacctg gcagacagca attttgatat cgccagcgtc
601 gcacagcatg tttgcttgtc gccgtcgcgt ctgtcacatc ttttccgcca gcagttaggg
661 attagcgtct taagctggcg cgaggaccaa cgtatcagcc aggcgaagct gcttttgagc
721 accacccgga tgcctatcgc caccgtcggt cgcaatgttg gttttgacga tcaactctat
781 ttctcgcggg tatttaaaaa atgcaccggg gccagcccga gcgagttccg tgccggttgt
841 gaagaaaaag tgaatgatgt agccgtcaag ttgtcataaa cagtcagtag ggccctggct
901 gttttggcgg atgagagaag attttcagcc tgatacagat taaatcagaa cgcagaagcg
961 gtctgataaa acagaatttg cctggcggca gtagcgcggt ggtcccacct gaccccatgc
1021 cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc catgcgagag
1081 tagggaactg ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
1141 tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat
1201 ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc
1261 aggcatcaaa ttaagcagaa ggccatcctg acggatggcc tttttgcgtt tctacaaact
1321 cgctgaggac attggctccg tgatgaaatt cgccacttgt tagtgtaatt cgctaactca
1381 tcctggcatg ttgctgttga ttcttcaatc agatctttat aaatcaaaaa gataaaaaat
1441 tggcacgcaa attgtattaa cagttcatcc ctatcagtga tagagagcag gacaatcctg
1501 aacgcagaaa tcaagaggac aacattacct gcaggtgcag taaaggaaaa aaaaaatgaa
1561 aaaattatta ttcgcaattc ctttagttgt tcctttctat tctcactccg ctgaaactgt
1621 tgaaagttgt ttagcaaaac cccatacaga aaattcattt actaacgtct ggaaagacga
1681 caaaacttta gatcgttacg ctaactatga gggctgtctg tggaatgcta caggcgttgt
1741 agtttgtact ggtgacgaaa ctcagtgtta cggtacatgg gttcctattg ggcttgctat
1801 ccctgaaaat gagggtggtg gctctgaggg tggcggttct gagggtggcg gttctgaggg
1861 tggcggtact aaacctcctg agtacggtga tacacctatt ccgggctata cttatatcaa
1921 ccctctcgac ggcacttatc cgcctggtac tgagcaaaac cccgctaatc ctaatccttc
1981 tcttgaggag tctcagcctc ttaatacttt catgtttcag aataataggt tccgaaatag
2041 gcagggggca ttaactgttt atacgggcac tgttactcaa ggcactgacc ccgttaaaac
2101 ttattaccag tacactcctg tatcatcaaa agccatgtat gacgcttact ggaacggtaa
2161 attcagagac tgcgctttcc attctggctt taatgaggat ccattcgttt gtgaatatca
2221 aggccaatcg tctgacctgc ctcaacctcc tgtcaatgct ggcggcggct ctggtggtgg
2281 ttctggtggc ggctctgagg gtggtggctc tgagggtggc ggttctgagg gtggcggctc
2341 tgagggaggc ggttccggtg gtggctctgg ttccggtgat tttgattatg aaaagatggc
2401 aaacgctaat aagggggcta tgaccgaaaa tgccgatgaa aacgcgctac agtctgacgc
2461 taaaggcaaa cttgattctg tcgctactga ttacggtgct gctatcgatg gtttcattgg
2521 tgacgtttcc ggccttgcta atggtaatgg tgctactggt gattttgctg gctctaattc
2581 ccaaatggct caagtcggtg acggtgataa ttcaccttta atgaataatt tccgtcaata
2641 tttaccttcc ctccctcaat cggttgaatg tcgccctttt gtctttggcg ctggtaaacc
2701 ttacgagttc agtatcgact gcgataagat caacctgttc cgcggtgtct ttgcgtttct
2761 tttatatgtt gccaccttta tgtatgtatt ttctacgttt gctaacatac tgcgtaataa
2821 ggagtcttaa agggtcccta agtctcctca gcaaaacgaa aggcccagtc tttcgactga
2881 gcctttcgtt ttatttgacc ggatgtcctc ttcctcctgg gctgacttca ggtgctacat
2941 ttgaagagat aaattgcact gaaatctaga gcggttcagt agaaaagatc aaaggatctt
3001 cttgagatcc tttttttctg cgcgtaatct tttgccctgt aaacgaaaaa accacctggg
3061 gaggtggttt gatcgaaggt taagtcagtt ggggaactgc ttaaccgtgg taactggctt
3121 tcgcagagca cagcaaccaa atctgtcctt ccagtgtagc cggactttgg cgcacacttc
3181 aagagcaacc gcgtgtttag ctaaacaaat cctctgcgaa ctcccagtta ccaatggctg
3241 ctgccagtgg cgttttaccg tgcttttccg ggttggactc aagtgaacag ttaccggata
3301 aggcgcagca gtcgggctga acggggagtt cttgcttaca gcccagcttg gagcgaacga
3361 cctacaccga gccgagatac cagtgtgtga gctatgagaa agcgccacac ttcccgtaag
3421 ggagaaaggc ggaacaggta tccggtaaac ggcagggtcg gaacaggaga gcgcaagagg
3481 gagcgacccg ccggaaacgg tggggatctt taagtcctgt cgggtttcgc ccgtactgtc
3541 agattcatgg ttgagcctca cggctcccac agatgcaccg gaaaagcgtc tgtttatgtg
3601 aactctggca ggagggcgga gcctatggaa aaacgccacc ggcgcggccc tgctgttttg
3661 cctcacatgt tagtcccctg cttatccacg gaatctgtgg gtaactttgt atgtgtccgc
3721 agcgcccgcc gcagtctcac gcccggagcg tagcgaccga gtgacggcct atttggcttg
3781 gcacgtaaga ggttccaact ttcaccataa tgaaataaga tcactaccgg gcgtattttt
3841 tgagttatcg agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata
3901 taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt
3961 tgctaaatgt acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt
4021 aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa
4081 tgctcatccg gagttccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt
4141 tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga
4201 ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg
4261 tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa
4321 tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc
4381 ccccgttttc actatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc
4441 gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt
4501 acaacagtac tgcgatgagt ggcagggcgg ggcgtaaacg ccatgggcat gtagtcaaaa
4561 gcctccggtc ggaggctttt gacttggctg aggagtgccg ttaattaagt ttaaaccgcc
4621 ggagatttgg tggtgtgcag gattttaacc tggatcatga tagacagttc agaacgacgt
4681 ttttccgcac gtttcagggt tttttccagc caacggtttt cgttcagctg gttgtgagaa
4741 gactggatga agattttacg gcagcactgg tagtgttcag aaaccataac gttcagacca
4801 acaccgttgt cacgcaggtt ccacagaccg atctggttac gcgcgttttt ttcgtagtac
4861 agtttgcacg cccagatttt cagggtgtga ccgttaccac gcagttcctg gttgtaccat
4921 tccaggattt tttccgcgca gtccgcgcac ggagaccaag aagagtacca gttgatggtg
4981 aactgacccg ggttgtcacg caggtattct tcaactttac ggatagagaa gatttccgcg
5041 tggataccac gttcggtacc agactgcggt ttgttaaccg cgtaacccca gaagcacgca
5101 cgacgttcac cacgacgttt cagttcgaac agaacgtagc aacggtgaga aacagatttt
5161 ttgttgttga agaactgttt tttgaaggtg tagatgtcca gtttttcgtg gatacgaacg
5221 tattccgcgt cggtcatctt cagattccgg tttacttaat ctcgacttat aacattttaa
5281 ttttattttc tccattactg tcttgaatta ctaaagccca aggtttatat tctggagcat
5341 ctgaagttaa tagcattaca ttttcatctg tactttcatc ataagcagta tgaactaaaa
5401 tatcactttc tggtttattc ccaattactt cctctacttc ttctggtaac attagaattg
5461 attcttgaat cactagttgt tttcctgttt ctttttcaat gatgtcagat aaatttgtca
5521 tgttgaaatt cctcctaatt tttgtattat tagctcatcg cttcgagaac cacaccgtct
5581 tgttccatct ggtcgagacg ggagagcaat ttgcgggtga tttgctcgag ctgatctttt
5641 tctgttgtgc tgagcgcgga ccagagttga tgcaggcagt tatgctgcgg tggtaaaacc
5701 tcgcgcaaaa actcgtgacc tttttccgtt aattgcagat gcaggcagcg gcgatcgtta
5761 tcgctttcac gacgttcgat ccaaccgcgt ttttccagtt catcggcaat acgcgtcgcg
5821 ttggtacggg atgatccaag agcacaactt aattcagaag gctgaatact gtggttttcc
5881 tgagactcca gcgtaatcaa cgccataaac aacgtctcgt taattccctg agccttcagc
5941 attttattgc ggttctccag cagcttgctt tgcatgtgca tgcaaagacg agtcagaagg
6001 atctcctgat aaggaaaatc ttcgtggcgg ctggcgcgaa attttagcat ttgttcaatg
6061 ggcgtaaacg aactatccat ttgggtatga cctcattaat ttcagccgtt agatagttcc
6121 gcaaaccttc tcaatcaatt ccgccgggaa ttgcatcgac tgcatgatgt gttcgatcat
6181 gctgcattta cggccggtgt tggtgttggt gatcacccaa tacggcgtgc ctggcacatg
6241 tttcggcttg gtctgattac catttttcag cagcgtttgt tcatctgccg caaagtaaac
6301 gcgtgtacga ccgtgcaacg attccgttgc ttcggcaaac gcctgggcgt caagagaata
6361 tagtgtagac aacagcagca taaagcgatt gaccgctcgc ttttgctctg cgtattcatc
6421 cgaaagcaga agttcacgca ttgcgcgaac cttgtcttta atcgttttga ccggcttcgc
6481 ttcgacgata gcaggtgacg caacgcgaac ctctttcgtc accggagcag caggctgtga
6541 tgcggcggaa aatttcaaca tacgccgtaa aatgtcggat gcgctctcgc cgatatgctt
6601 agtgtggctg gcaatatagc tgtagagttc atcatcaact tcaatcgttt tcatcttaat
6661 ccagtgcagt gtcttagctg aattattttt tcgcgggtga aacgactcct ggtttgtaca
6721 aagccagcag ttcgtccacc tttttacgtg tgccgccgtt gctgcttata ctgcgtcgaa
6781 ctttgacgac atgcaatttt gcgcgctgat accactcacg cgttaacatc gtatcgtgat
6841 tggagatcag cactggaata tggcgctcaa ccagaccttc ggcgatctcc gccagatgcg
6901 cttgttgttc aagcgtaaaa ctgtttgtgt gatacgccgt aaagttggcg gtcgcagaca
6961 gcggtgcata aggcggatcg caatagacga cggatgcatc atctgcgcgc gccatgctat
7021 cggcgtaaga ctcacaatag aaaaaggcat tctgcgcttt ttcagcgaag tgatacaact
7081 ctgcttccgg gaaatagggt tttttgtagc ggccgaacgg cacgttaaac tcaccgcgca
7141 gattgtaacg acacaggccg ttgtaaccgt agcggttcaa atataaaaac agtaccgccc
7201 gacggaacgg atcctggctt ttgttgaact cttcgcggaa ctgatagtaa acctcggcgc
7261 aatttgtttc gggaacaaac agctcgcgtg cggcctgtac gtactcatca gtacgcatct
7321 tcacaatgtt atagagactg atcaggtcgc tattgatatc ggcaaggata taacgagaaa
7381 agtcggtgtt gagaaacacc gacccggcac ctacaaaagg ctcaaccaga cattcgccct
7441 tgggcaaatg ccgtttaata tcatcaagca ggggatactt gccccctgcc cacttcaaaa
7501 aagcgcgatt tttcttcatg ggttatgtcc ggtttactga tattaaatta tgctcgccag
7561 aggcaacttc cgcctttctt ctgcaccaga tcgagacggg cttcatgagc tgcaatctct
7621 tcatctgtcg caaaaacaac gcgtaactta cttgcctgac gtacaatgcg ctgaattgtt
7681 gcttcacctt gttgctgttg tgtctctcct tccatcgcaa aagccatcga cgtttgacca
7741 ccggtcatcg ccagataaac ttccgcaagg atctgggcat cgagtaatgc cccgtgcagc
7801 gttcgtttac tgttatctat ttcgtagcga gcacataacg catcgaggct gttgcgctta
7861 ccgggaaaca ttttcctcgc caccgcaagg ctatcggtga ccttacagaa agtattggtc
7921 ttcggaatat cgcgcttaag caacgaaaac tcgtagtcca taaagccgat atcgaacgct
7981 gcgttatgga tcaccaactc cgcgccgcga atatagtcca tgaactcatc ggctacttcg
8041 gcaaacgtgg gcttatcgag caaaaattca tcggcaatac catgtacgcc aaaggcttcc
8101 ggatccacca gccgatcggg tttgagataa acatggaagt tattgcccgt caggcgacgg
8161 ttcaccactt caacggcacc aatctcaatg atcttgtggc cttcatagtg cgcaccaatc
8221 tggttcatac cggtggttgc ggtagcgaga acgatctggc gtgtaattgc agtgctcata
8281 gcggtcattt atgtcagact tgagttgtac gcgtccaaaa aaacgggtat ggagaaacag
8341 tagagagttg cgataaaaag cgtcaggtag gatccgctaa tcttatggat aaaaatgcta
8401 tggcatagca aagtgtgacg ccgtgcaaat aatcaatgtg gacttttctg ccgtgattat
8461 agacactttt gttacgcgtt tttgtcatgg ctttggtccc gctttgttac agaatgcttt
8521 taataagcgg ggttaccggt tgggttagcg agaagagcca gtaaaagacg cagtgacggc
8581 aatgtctgat gcaatatgga caattggttt cttctctgaa tggtgggagt atgaaaagt
//
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