| 质粒名称: | pAAV2/7 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 112863 |
| 存储实验室: | James M. Wilson |
|
载体骨架基本信息 |
|
| 载体名称: | pN1 |
| 载体出品公司: | |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | AAV |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Rep2/Cap7 |
| 物种来源: | AAV |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | Truncated P5 |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | KS primer |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pAAV2/7 was a gift from James M. Wilson (Addgene plasmid # 112863 ; http://n2t.net/addgene:112863 ; RRID:Addgene_112863) |
买家导航
pAAV27载体基本信息
pAAV27载体图谱质粒图谱和多克隆位点信息
pAAV27载体简介
pAAV27载体序列
LOCUS pAAV2/7 7333 bp ds-DNA circular SYN 07-AUG-2018
DEFINITION AAV packaging plasmid expressing Rep/Cap genes.
ACCESSION .
VERSION .
KEYWORDS pAAV2/7
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7333)
TITLE AAV packaging plasmids
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 7333)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 7, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..7333
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 11..28
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 12..28
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 38..57
/label=T7
/note="T7 promoter, forward primer"
promoter 38..56
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 85..101
/label=pBluescriptKS
/note="For pBluescript vector"
primer_bind 86..102
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4557..4577)
/label=T3
/note="T3 promoter, forward primer"
promoter complement(4557..4575)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(4596..4612)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4596..4612)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(4609..4631)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4620..4636
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4644..4674)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4689..4710
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(4827..4844)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4998..5586)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5078..5097)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5757..6617)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6380..6399
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6618..6722)
/gene="bla"
/label=AmpR promoter
rep_origin complement(6748..7203)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6885..6906)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 7097..7116
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 7330..19
/label=M13/pUC Forward
/note="In lacZ gene"
ORIGIN
1 gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac tatagggcga
61 attgggtacc gggccccccc tcgatcgagg tcgacggtat cgggggagct cgcagggtct
121 ccattttgaa gcgggaggtt tgaacgcgca gccgccatgc cggggtttta cgagattgtg
181 attaaggtcc ccagcgacct tgacgagcat ctgcccggca tttctgacag ctttgtgaac
241 tgggtggccg agaaggaatg ggagttgccg ccagattctg acatggatct gaatctgatt
301 gagcaggcac ccctgaccgt ggccgagaag ctgcagcgcg actttctgac ggaatggcgc
361 cgtgtgagta aggccccgga ggctcttttc tttgtgcaat ttgagaaggg agagagctac
421 ttccacatgc acgtgctcgt ggaaaccacc ggggtgaaat ccatggtttt gggacgtttc
481 ctgagtcaga ttcgcgaaaa actgattcag agaatttacc gcgggatcga gccgactttg
541 ccaaactggt tcgcggtcac aaagaccaga aatggcgccg gaggcgggaa caaggtggtg
601 gatgagtgct acatccccaa ttacttgctc cccaaaaccc agcctgagct ccagtgggcg
661 tggactaata tggaacagta tttaagcgcc tgtttgaatc tcacggagcg taaacggttg
721 gtggcgcagc atctgacgca cgtgtcgcag acgcaggagc agaacaaaga gaatcagaat
781 cccaattctg atgcgccggt gatcagatca aaaacttcag ccaggtacat ggagctggtc
841 gggtggctcg tggacaaggg gattacctcg gagaagcagt ggatccagga ggaccaggcc
901 tcatacatct ccttcaatgc ggcctccaac tcgcggtccc aaatcaaggc tgccttggac
961 aatgcgggaa agattatgag cctgactaaa accgcccccg actacctggt gggccagcag
1021 cccgtggagg acatttccag caatcggatt tataaaattt tggaactaaa cgggtacgat
1081 ccccaatatg cggcttccgt ctttctggga tgggccacga aaaagttcgg caagaggaac
1141 accatctggc tgtttgggcc tgcaactacc gggaagacca acatcgcgga ggccatagcc
1201 cacactgtgc ccttctacgg gtgcgtaaac tggaccaatg agaactttcc cttcaacgac
1261 tgtgtcgaca agatggtgat ctggtgggag gaggggaaga tgaccgccaa ggtcgtggag
1321 tcggccaaag ccattctcgg aggaagcaag gtgcgcgtgg accagaaatg caagtcctcg
1381 gcccagatag acccgactcc cgtgatcgtc acctccaaca ccaacatgtg cgccgtgatt
1441 gacgggaact caacgacctt cgaacaccag cagccgttgc aagaccggat gttcaaattt
1501 gaactcaccc gccgtctgga tcatgacttt gggaaggtca ccaagcagga agtcaaagac
1561 tttttccggt gggcaaagga tcacgtggtt gaggtggagc atgaattcta cgtcaaaaag
1621 ggtggagcca agaaaagacc cgcccccagt gacgcagata taagtgagcc caaacgggtg
1681 cgcgagtcag ttgcgcagcc atcgacgtca gacgcggaag cttcgatcaa ctacgcagac
1741 aggtaccaaa acaaatgttc tcgtcacgtg ggcatgaatc tgatgctgtt tccctgcaga
1801 caatgcgaga gaatgaatca gaattcaaat atctgcttca ctcacggaca gaaagactgt
1861 ttagagtgct ttcccgtgtc agaatctcaa cccgtttctg tcgtcaaaaa ggcgtatcag
1921 aaactgtgct acattcatca tatcatggga aaggtgccag acgcttgcac tgcctgcgat
1981 ctggtcaatg tggatttgga tgactgcatc tttgaacaat aaatgattta aatcaggtat
2041 ggctgccgat ggttatcttc cagattggct cgaggacaac ctctctgagg gcattcgcga
2101 gtggtgggac ctgaaacctg gagccccgaa acccaaagcc aaccagcaaa agcaggacaa
2161 cggccggggt ctggtgcttc ctggctacaa gtacctcgga cccttcaacg gactcgacaa
2221 gggggagccc gtcaacgcgg cggacgcagc ggccctcgag cacgacaagg cctacgacca
2281 gcagctcaaa gcgggtgaca atccgtacct gcggtataac cacgccgacg ccgagtttca
2341 ggagcgtctg caagaagata cgtcatttgg gggcaacctc gggcgagcag tcttccaggc
2401 caagaagcgg gttctcgaac ctctcggtct ggttgaggaa ggcgctaaga cggctcctgc
2461 aaagaagaga ccggtagagc cgtcacctca gcgttccccc gactcctcca cgggcatcgg
2521 caagaaaggc cagcagcccg ccagaaagag actcaatttc ggtcagactg gcgactcaga
2581 gtcagtcccc gaccctcaac ctctcggaga acctccagca gcgccctcta gtgtgggatc
2641 tggtacagtg gctgcaggcg gtggcgcacc aatggcagac aataacgaag gtgccgacgg
2701 agtgggtaat gcctcaggaa attggcattg cgattccaca tggctgggcg acagagtcat
2761 taccaccagc acccgaacct gggccctgcc cacctacaac aaccacctct acaagcaaat
2821 ctccagtgaa actgcaggta gtaccaacga caacacctac ttcggctaca gcaccccctg
2881 ggggtatttt gactttaaca gattccactg ccacttctca ccacgtgact ggcagcgact
2941 catcaacaac aactggggat tccggcccaa gaagctgcgg ttcaagctct tcaacatcca
3001 ggtcaaggag gtcacgacga atgacggcgt tacgaccatc gctaataacc ttaccagcac
3061 gattcaggta ttctcggact cggaatacca gctgccgtac gtcctcggct ctgcgcacca
3121 gggctgcctg cctccgttcc cggcggacgt cttcatgatt cctcagtacg gctacctgac
3181 tctcaacaat ggcagtcagt ctgtgggacg ttcctccttc tactgcctgg agtacttccc
3241 ctctcagatg ctgagaacgg gcaacaactt tgagttcagc tacagcttcg aggacgtgcc
3301 tttccacagc agctacgcac acagccagag cctggaccgg ctgatgaatc ccctcatcga
3361 ccagtacttg tactacctgg ccagaacaca gagtaaccca ggaggcacag ctggcaatcg
3421 ggaactgcag ttttaccagg gcgggccttc aactatggcc gaacaagcca agaattggtt
3481 acctggacct tgcttccggc aacaaagagt ctccaaaacg ctggatcaaa acaacaacag
3541 caactttgct tggactggtg ccaccaaata tcacctgaac ggcagaaact cgttggttaa
3601 tcccggcgtc gccatggcaa ctcacaagga cgacgaggac cgctttttcc catccagcgg
3661 agtcctgatt tttggaaaaa ctggagcaac taacaaaact acattggaaa atgtgttaat
3721 gacaaatgaa gaagaaattc gtcctactaa tcctgtagcc acggaagaat acgggatagt
3781 cagcagcaac ttacaagcgg ctaatactgc agcccagaca caagttgtca acaaccaggg
3841 agccttacct ggcatggtct ggcagaaccg ggacgtgtac ctgcagggtc ccatctgggc
3901 caagattcct cacacggatg gcaactttca cccgtctcct ttgatgggcg gctttggact
3961 taaacatccg cctcctcaga tcctgatcaa gaacactccc gttcccgcta atcctccgga
4021 ggtgtttact cctgccaagt ttgcttcgtt catcacacag tacagcaccg gacaagtcag
4081 cgtggaaatc gagtgggagc tgcagaagga aaacagcaag cgctggaacc cggagattca
4141 gtacacctcc aactttgaaa agcagactgg tgtggacttt gccgttgaca gccagggtgt
4201 ttactctgag cctcgcccta ttggcactcg ttacctcacc cgtaatctgt aattgcatgt
4261 taatcaataa accggttgat tcgtttcagt tgaactttgg tctctgcgaa gggcgaattc
4321 gtttaaacct gcaggactag aggtcctgta ttagaggtca cgtgagtgtt ttgcgacatt
4381 ttgcgacacc atgtggtcac gctgggtatt taagcccgag tgagcacgca gggtctccat
4441 tttgaagcgg gaggtttgaa cgcgcagccg ccaagccgaa ttctgcagat atccatcaca
4501 ctggcggccg ctcgactaga gcggccgcca ccgcggtgga gctccagctt ttgttccctt
4561 tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
4621 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
4681 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
4741 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
4801 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
4861 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
4921 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
4981 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
5041 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
5101 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
5161 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
5221 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
5281 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
5341 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
5401 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
5461 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
5521 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
5581 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
5641 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
5701 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
5761 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
5821 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
5881 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
5941 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
6001 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
6061 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
6121 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
6181 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
6241 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
6301 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
6361 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
6421 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
6481 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
6541 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
6601 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
6661 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
6721 cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg
6781 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
6841 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
6901 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
6961 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
7021 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
7081 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
7141 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
7201 cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
7261 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
7321 cagggttttc cca
//
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