| 质粒名称: | FUtdTW |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 22478 |
| 存储实验室: | Connie Cepko |
|
载体骨架基本信息 |
|
| 载体名称: | FUGW |
| 载体出品公司: | I. Verma, Salk |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 9165 bp |
| 完整质粒大小: | 10565 bp |
| 载体类型: | 哺乳细胞表达，Lentiviral |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | tandem dimer tomato |
| 物种来源: | Discosoma sp |
| 插入大小: | 1400 bp |
| 基因又名: |
tdtomato tomato |
| 启动子: | UbC |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | XbaI, BamHI(未破坏) |
| 3' 克隆位点: | EcoRI (未破坏) |
| 5' 测序引物 | hUBCpro-F (5'-TGAAGCTCCGGTTTTGAACT-3') |
| 3' 测序引物 | WPRE-R |
|
文献引用方法 |
|
| 材料和方法部分: | FUtdTW was a gift from Connie Cepko (爱迪基因 plasmid # 22478) |
| 参考文献部分: | Retinal progenitor cells can produce restricted subsets of horizontal cells. Rompani SB, Cepko CL. Proc Natl Acad Sci U S A. 2008 Jan 8. 105(1):192-7. 10.1073/pnas.0709979104 PubMed 18162542 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS FUtdTW 10638 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS FUtdTW
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10638)
AUTHORS Rompani SB, Cepko CL
TITLE Retinal progenitor cells can produce restricted subsets of
horizontal cells.
JOURNAL Proc Natl Acad Sci U S A. 2008 Jan 8. 105(1):192-7.
PUBMED 18162542
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|FUtdTW|
FEATURES Location/Qualifiers
source 1..10638
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(44..63)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 616..814
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 769..789
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 832..1012
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 1059..1184
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1677..1910
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 2437..2554
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2619..3830
/label=UbC promoter
/note="human ubiquitin C promoter"
primer_bind 3667..3686
/label=hUBCpro-F
/note="Human Ubiquitin C (UbC) promoter, forward primer"
regulatory 3866..3875
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 3872..5302
/codon_start=1
/product="tandem dimeric (pseudo-monomeric) derivative of
DsRed (Shaner et al., 2004)"
/label=tdTomato
/note="mammalian codon-optimized"
/translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
RSEGRHHLFLYGMDELYK"
misc_feature 5327..5915
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(5380..5400)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5798..5809)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(5918..5934)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5919..5935)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 6440..6620
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
primer_bind complement(6646..6663)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 6652..6876
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 6922..7350
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(7009..7028)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 7219..7240
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(7359..7379)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 7364..7693
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 7544..7679
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 7606..7625
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 7741..7788
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 7807..8181
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 8311..8432
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(8348..8367)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 8402..8421
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(8481..8497)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8481..8497)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(8494..8516)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 8505..8521
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(8529..8559)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8574..8595
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(8712..8729)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8883..9471)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8963..8982)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(9642..10502)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 10265..10284
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(10503..10607)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcggc actgcgtgcg
2401 ccaattctgc agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
2461 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
2521 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
2581 tccagtttgg ttaattaacc cgtgtcggct ccagatctgg cctccgcgcc gggttttggc
2641 gcctcccgcg ggcgcccccc tcctcacggc gagcgctgcc acgtcagacg aagggcgcag
2701 cgagcgtcct gatccttccg cccggacgct caggacagcg gcccgctgct cataagactc
2761 ggccttagaa ccccagtatc agcagaagga cattttagga cgggacttgg gtgactctag
2821 ggcactggtt ttctttccag agagcggaac aggcgaggaa aagtagtccc ttctcggcga
2881 ttctgcggag ggatctccgt ggggcggtga acgccgatga ttatataagg acgcgccggg
2941 tgtggcacag ctagttccgt cgcagccggg atttgggtcg cggttcttgt ttgtggatcg
3001 ctgtgatcgt cacttggtga gtagcgggct gctgggctgg ccggggcttt cgtggccgcc
3061 gggccgctcg gtgggacgga agcgtgtgga gagaccgcca agggctgtag tctgggtccg
3121 cgagcaaggt tgccctgaac tgggggttgg ggggagcgca gcaaaatggc ggctgttccc
3181 gagtcttgaa tggaagacgc ttgtgaggcg ggctgtgagg tcgttgaaac aaggtggggg
3241 gcatggtggg cggcaagaac ccaaggtctt gaggccttcg ctaatgcggg aaagctctta
3301 ttcgggtgag atgggctggg gcaccatctg gggaccctga cgtgaagttt gtcactgact
3361 ggagaactcg gtttgtcgtc tgttgcgggg gcggcagtta tggcggtgcc gttgggcagt
3421 gcacccgtac ctttgggagc gcgcgccctc gtcgtgtcgt gacgtcaccc gttctgttgg
3481 cttataatgc agggtggggc cacctgccgg taggtgtgcg gtaggctttt ctccgtcgca
3541 ggacgcaggg ttcgggccta gggtaggctc tcctgaatcg acaggcgccg gacctctggt
3601 gaggggaggg ataagtgagg cgtcagtttc tttggtcggt tttatgtacc tatcttctta
3661 agtagctgaa gctccggttt tgaactatgc gctcggggtt ggcgagtgtg ttttgtgaag
3721 ttttttaggc accttttgaa atgtaatcat ttgggtcaat atgtaatttt cagtgttaga
3781 ctagtaaatt gtccgctaaa ttctggccgt ttttggcttt tttgttagac gaagcttggg
3841 ctgcaggtcg actctagagg atcccgccac catggtgagc aagggcgagg aggtcatcaa
3901 agagttcatg cgcttcaagg tgcgcatgga gggctccatg aacggccacg agttcgagat
3961 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
4021 caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttca tgtacggctc
4081 caaggcgtac gtgaagcacc ccgccgacat ccccgattac aagaagctgt ccttccccga
4141 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggtctggtga ccgtgaccca
4201 ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt
4261 cccccccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg
4321 cctgtacccc cgcgacggcg tgctgaaggg cgagatccac caggccctga agctgaagga
4381 cggcggccac tacctggtgg agttcaagac catctacatg gccaagaagc ccgtgcaact
4441 gcccggctac tactacgtgg acaccaagct ggacatcacc tcccacaacg aggactacac
4501 catcgtggaa cagtacgagc gctccgaggg ccgccaccac ctgttcctgg ggcatggcac
4561 cggcagcacc ggcagcggca gctccggcac cgcctcctcc gaggacaaca acatggccgt
4621 catcaaagag ttcatgcgct tcaaggtgcg catggagggc tccatgaacg gccacgagtt
4681 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
4741 ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc agttcatgta
4801 cggctccaag gcgtacgtga agcaccccgc cgacatcccc gattacaaga agctgtcctt
4861 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggtc tggtgaccgt
4921 gacccaggac tcctccctgc aggacggcac gctgatctac aaggtgaaga tgcgcggcac
4981 caacttcccc cccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctccac
5041 cgagcgcctg tacccccgcg acggcgtgct gaagggcgag atccaccagg ccctgaagct
5101 gaaggacggc ggccactacc tggtggagtt caagaccatc tacatggcca agaagcccgt
5161 gcaactgccc ggctactact acgtggacac caagctggac atcacctccc acaacgagga
5221 ctacaccatc gtggaacagt acgagcgctc cgagggccgc caccacctgt tcctgtacgg
5281 catggacgag ctgtacaagt aagaattcga tatcaagctt atcgataatc aacctctgga
5341 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
5401 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
5461 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
5521 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
5581 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
5641 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
5701 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
5761 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
5821 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
5881 gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgagacct
5941 agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg
6001 gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag
6061 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
6121 ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac
6181 acaaggctac ttccctgatt ggcagaacta cacaccaggg ccagggatca gatatccact
6241 gacctttgga tggtgctaca agctagtacc agttgagcaa gagaaggtag aagaagccaa
6301 tgaaggagag aacacccgct tgttacaccc tgtgagcctg catgggatgg atgacccgga
6361 gagagaagta ttagagtgga ggtttgacag ccgcctagca tttcatcaca tggcccgaga
6421 gctgcatccg gactgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
6481 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
6541 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
6601 agtgtggaaa atctctagca gggcccgttt aaacccgctg atcagcctcg actgtgcctt
6661 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
6721 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
6781 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
6841 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
6901 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
6961 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
7021 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
7081 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
7141 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
7201 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
7261 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
7321 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
7381 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
7441 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
7501 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
7561 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
7621 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
7681 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt
7741 gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac
7801 taaaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga
7861 gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc
7921 gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg
7981 ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg
8041 tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc
8101 gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc
8161 gtggccgagg agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat
8221 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
8281 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
8341 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
8401 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
8461 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
8521 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
8581 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
8641 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
8701 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
8761 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
8821 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
8881 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
8941 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
9001 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
9061 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
9121 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
9181 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
9241 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
9301 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
9361 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
9421 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
9481 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
9541 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
9601 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
9661 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
9721 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
9781 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
9841 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
9901 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
9961 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
10021 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
10081 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
10141 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
10201 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
10261 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
10321 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
10381 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
10441 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
10501 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
10561 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
10621 aaagtgccac ctgacgtc
//
