pMXs-hOCT3/4

价格:1200元

联系方式:I47-825O-882O

相关技术服务:质粒构建    基因合成    质粒大提

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载体基本信息

质粒名称: pMXs-hOCT3/4 
出品公司: 爱迪基因
目录编号: 17217
存储实验室: Shinya Yamanaka

载体骨架基本信息
载体名称: pMXs
载体抗性: 氨苄霉素
空载体大小: 4600 bp
载体类型: 哺乳细胞表达
高拷贝/低拷贝: 高拷贝
生长条件: 37 ℃

插入基因信息
插入基因名称: OCT3/4
物种来源: H. sapiens (human)
插入大小: 1100 bp
基因突变: None
克隆方法: 限制性内切酶
5' 克隆位点: EcoRI(未破坏)
3' 克隆位点: EcoRI(未破坏)
5' 测序引物 pMX-S1811
3' 测序引物  

文献引用方法
材料和方法部分: pMXs-hOCT3/4 was a gift from Shinya Yamanaka (Addgene plasmid # 17217)
参考文献部分: Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S. Cell. 2007 Nov 30. 131(5):861-72. 10.1016/j.cell.2007.11.019 PubMed 18035408

载体图谱质粒图谱和多克隆位点信息

pMXs-hOCT3/4载体图谱

载体简介

载体序列

LOCUS       pMXs-hOCT3/4 Sequencing Result	5856 bp 	DNA	SYN
DEFINITION  pMXs-hOCT3/4 Sequencing Result
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..5856
                     /organism="pMXs-hOCT3/4 Sequencing Result"
                     /mol_type="other DNA"
     gene            complement(674..1534)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(674..1534)
                     /label="ORF frame 3"
     promoter        complement(1576..1604)
                     /label="AmpR_promoter"
     misc_feature    1669..2262
                     /label="3MoMuLV_LTR"
     misc_feature    1711..2262
                     /label="5_LTR2"
     misc_feature    2262..3543
                     /label="psi_plus_pack2"
     misc_feature    2744..3226
                     /label="gag"
     misc_feature    3087..3109
                     /label="MSCV_primer"
     misc_feature    3087..3109
                     /label="pLXSN_5_primer"
     misc_feature    3125..3141
                     /label="pBABE_5_primer"
     misc_feature    3479..3495
                     /label="pBMN_5_primer"
     CDS             3602..4684
                     /label="ORF frame 2"
     misc_feature    4887..1033
                     /label="3MoMuLV_LTR"
     misc_feature    4929..5468
                     /label="5_LTR2"
     rep_origin      complement(5763..526)
                     /label="pBR322_origin"
ORIGIN
    1 TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG
   61 CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC
  121 TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC
  181 GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC
  241 CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG
  301 AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA
  361 AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT
  421 AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG
  481 CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT
  541 GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG
  601 ATCTTCACCT AGATCCTTTT GCGGCCGGCC GCAAATCAAT CTAAAGTATA TATGAGTAAA
  661 CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT
  721 TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT
  781 TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT
  841 TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT
  901 CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA
  961 ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG
 1021 GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT
 1081 TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG
 1141 CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG
 1201 TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC
 1261 GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA
 1321 CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC
 1381 CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT
 1441 TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG
 1501 GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA
 1561 GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA
 1621 AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGCATAA TGAAAGACCC
 1681 CACCTGTAGG TTTGGCAAGC TAGCTTAAGT AACGCCATTT TGCAAGGCAT GGAAAAATAC
 1741 ATAACTGAGA ATAGAAAAGT TCAGATCAAG GTCAGGAACA GATGGAACAG CTGAATATGG
 1801 GCCAAACAGG ATATCTGTGG TAAGCAGTTC CTGCCCCGGC TCAGGGCCAA GAACAGATGG
 1861 AACAGCTGAA TATGGGCCAA ACAGGATATC TGTGGTAAGC AGTTCCTGCC CCGGCTCAGG
 1921 GCCAAGAACA GATGGTCCCC AGATGCGGTC CAGCCCTCAG CAGTTTCTAG AGAACCATCA
 1981 GATGTTTCCA GGGTGCCCCA AGGACCTGAA ATGACCCTGT GCCTTATTTG AACTAACCAA
 2041 TCAGTTCGCT TCTCGCTTCT GTTCGCGCGC TTCTGCTCCC CGAGCTCAAT AAAAGAGCCC
 2101 ACAACCCCTC ACTCGGCGCG CCAGTCCTCC GATTGACTGA GTCGCCCGGG TACCCGTGTA
 2161 TCCAATAAAC CCTCTTGCAG TTGCATCCGA CTTGTGGTCT CGCTGTTCCT TGGGAGGGTC
 2221 TCCTCTGAGT GATTGACTAC CCGTCAGCGG GGGTCTTTCA TTTGGGGGCT CGTCCGGGAT
 2281 CGGGAGACCC CTGCCCAGGG ACCACCGACC CACCACCGGG AGGTAAGCTG GCCAGCAACT
 2341 TATCTGTGTC TGTCCGATTG TCTAGTGTCT ATGACTGATT TTATGCGCCT GCGTCGGTAC
 2401 TAGTTAGCTA ACTAGCTCTG TATCTGGCGG ACCCGTGGTG GAACTGACGA GTTCGGAACA
 2461 CCCGGCCGCA ACCCTGGGAG ACGTCCCAGG GACTTCGGGG GCCGTTTTTG TGGCCCGACC
 2521 TGAGTCCAAA AATCCCGATC GTTTTGGACT CTTTGGTGCA CCCCCCTAAT AGGAGGGATA
 2581 TGTGGTTCTG GTAGGAGACG AGAACCTAAA ACAGTTCCCG CCTCCGTCTG AATTTTTGCT
 2641 TTCGGTTTGG GACCGAAGCC GCGCCGCGCG TCTTGTCTGC TGCAGCATCG TTCTGTGTTG
 2701 TCTCTGTCTG ACTGTGTTTC TGTATTTGTC TGAAAATTAG GGCCAGACTG TTACCACTCC
 2761 CTTAAGTTTG ACCTTAGGTC ACTGGAAAGA TGTCGAGCGG ATCGCTCACA ACCAGTCGGT
 2821 AGATGTCAAG AAGAGACGTT GGGTTACCTT CTGCTCTGCA GAATGGCCAA CCTTTAACGT
 2881 CGGATGGCCG CGAGACGGCA CCTTTAACCG AGACCTCATC ACCCAGGTTA AGATCAAGGT
 2941 CTTTTCACCT GGCCCGCATG GACACCCAGA CCAGGTCCCC TACATCGTGA CCTGGGAAGC
 3001 CTTGGCTTTT GACCCCCCTC CCTGGGTCAA GCCCTTTGTA CACCCTAAGC CTCCGCCTCC
 3061 TCTTCCTCCA TCCGCCCCGT CTCTCCCCCT TGAACCTCCT CGTTCGACCC CGCCTCGATC
 3121 CTCCCTTTAT CCAGCCCTCA CTCCTTCTCT AGGCGCCCCC ATATGGCCAT ATGAGATCTT
 3181 ATATGGGGCA CCCCCGCCCC TTGTAAACTT CCCTGACCCT GACATGACAA GAGTTACTAA
 3241 CAGCCCCTCT CTCCAAGCTC ACTTACAGGC TCTCTACTTA GTCCAGCACG AAGTCTGGAG
 3301 ACCTCTGGCG GCAGCCTACC AAGAACAACT GGACCGACCG GTGGTACCTC ACCCTTACCG
 3361 AGTCGGCGAC ACAGTGTGGG TCCGCCGACA CCAGACTAAG AACCTAGAAC CTCGCTGGAA
 3421 AGGACCTTAC ACAGTCCTGC TGACCACCCC CACCGCCCTC AAAGTAGACG GCATCGCAGC
 3481 TTGGATACAC GCCGCCCACG TGAAGGCTGC CGACCCCGGG GGTGGACCAT CCTCTAGACT
 3541 GCCGGATCTA GCTAGTTAAT TAAGGATCCC AGTGTGGTGG TACGGGAATT CGCCCTTCAC
 3601 CATGGCGGGA CACCTGGCTT CGGATTTCGC CTTCTCGCCC CCTCCAGGTG GTGGAGGTGA
 3661 TGGGCCAGGG GGGCCGGAGC CGGGCTGGGT TGATCCTCGG ACCTGGCTAA GCTTCCAAGG
 3721 CCCTCCTGGA GGGCCAGGAA TCGGGCCGGG GGTTGGGCCA GGCTCTGAGG TGTGGGGGAT
 3781 TCCCCCATGC CCCCCGCCGT ATGAGTTCTG TGGGGGGATG GCGTACTGTG GGCCCCAGGT
 3841 TGGAGTGGGG CTAGTGCCCC AAGGCGGCTT GGAGACCTCT CAGCCTGAGG GCGAAGCAGG
 3901 AGTCGGGGTG GAGAGCAACT CCGATGGGGC CTCCCCGGAG CCCTGCACCG TCACCCCTGG
 3961 TGCCGTGAAG CTGGAGAAGG AGAAGCTGGA GCAAAACCCG GAGGAGTCCC AGGACATCAA
 4021 AGCTCTGCAG AAAGAACTCG AGCAATTTGC CAAGCTCCTG AAGCAGAAGA GGATCACCCT
 4081 GGGATATACA CAGGCCGATG TGGGGCTCAC CCTGGGGGTT CTATTTGGGA AGGTATTCAG
 4141 CCAAACGACC ATCTGCCGCT TTGAGGCTCT GCAGCTTAGC TTCAAGAACA TGTGTAAGCT
 4201 GCGGCCCTTG CTGCAGAAGT GGGTGGAGGA AGCTGACAAC AATGAAAATC TTCAGGAGAT
 4261 ATGCAAAGCA GAAACCCTCG TGCAGGCCCG AAAGAGAAAG CGAACCAGTA TCGAGAACCG
 4321 AGTGAGAGGC AACCTGGAGA ATTTGTTCCT GCAGTGCCCG AAACCCACAC TGCAGCAGAT
 4381 CAGCCACATC GCCCAGCAGC TTGGGCTCGA GAAGGATGTG GTCCGAGTGT GGTTCTGTAA
 4441 CCGGCGCCAG AAGGGCAAGC GATCAAGCAG CGACTATGCA CAACGAGAGG ATTTTGAGGC
 4501 TGCTGGGTCT CCTTTCTCAG GGGGACCAGT GTCCTTTCCT CTGGCCCCAG GGCCCCATTT
 4561 TGGTACCCCA GGCTATGGGA GCCCTCACTT CACTGCACTG TACTCCTCGG TCCCTTTCCC
 4621 TGAGGGGGAA GCCTTTCCCC CTGTCTCTGT CACCACTCTG GGCTCTCCCA TGCATTCAAA
 4681 CTGAGGTAAG GGCGAATTCC AGCTGAGCGC CGGTCGCTAC CATTACCAGT TGGTCTGGTG
 4741 TCAAAAATAA TAATAACCGG GCAGGCCATG TCTGCCCGTA TTTCGCGTAA GGAAATCCAT
 4801 TATGTACTAT TTAAACTCGA GCGGCCGCCA GCACAGTGGT CGACGATAAA ATAAAAGATT
 4861 TTATTTAGTC TCCAGAAAAA GGGGGGAATG AAAGACCCCA CCTGTAGGTT TGGCAAGCTA
 4921 GCTTAAGTAA CGCCATTTTG CAAGGCATGG AAAAATACAT AACTGAGAAT AGAGAAGTTC
 4981 AGATCAAGGT CAGGAACAGA TGGAACAGCT GAATATGGGC CAAACAGGAT ATCTGTGGTA
 5041 AGCAGTTCCT GCCCCGGCTC AGGGCCAAGA ACAGATGGAA CAGCTGAATA TGGGCCAAAC
 5101 AGGATATCTG TGGTAAGCAG TTCCTGCCCC GGCTCAGGGC CAAGAACAGA TGGTCCCCAG
 5161 ATGCGGTCCA GCCCTCAGCA GTTTCTAGAG AACCATCAGA TGTTTCCAGG GTGCCCCAAG
 5221 GACCTGAAAT GACCCTGTGC CTTATTTGAA CTAACCAATC AGTTCGCTTC TCGCTTCTGT
 5281 TCGCGCGCTT CTGCTCCCCG AGCTCAATAA AAGAGCCCAC AACCCCTCAC TCGGGGCGCC
 5341 AGTCCTCCGA TTGACTGAGT CGCCCGGGTA CCCGTGTATC CAATAAACCC TCTTGCAGTT
 5401 GCATCCGACT TGTGGTCTCG CTGTTCCTTG GGAGGGTCTC CTCTGAGTGA TTGACTACCC
 5461 GTCAGCGGGG GTCTTTCACA TGCAGCATGT ATCAAAATTA ATTTGGTTTT TTTCTTAAGT
 5521 ATTTACATTA AATGGCCATA GTTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT
 5581 TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG
 5641 CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG
 5701 GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG
 5761 GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA
 5821 CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTA
//

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