pCRI005-pYFAC-riboB-PgpdA-dLbCpf1(D156R)-VPR-TtrpC

LOCUS       pCRI005-pYFAC-riboB-PgpdA-dLbCpf1(D156R)-VPR-TtrpC              25018 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Episomal expression of dLbCpf1(D156R)-VPR, variant for culture at 
            room temperature. Filamentous fungi vector with AMA1 and riboB 
            selection marker..
ACCESSION   .
VERSION     .
KEYWORDS    pCRI005-pYFAC-riboB-PgpdA-dLbCpf1(D156R)-VPR-TtrpC
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 25018)
  AUTHORS   Roux I, Woodcraft C, Hu J, Wolters R, Gilchrist CLM, Chooi YH
  TITLE     CRISPR-mediated activation of biosynthetic gene clusters for 
            bioactive molecule discovery in filamentous fungi.
  JOURNAL   ACS Synth Biol. 2020 Jun 11. doi: 10.1021/acssynbio.0c00197.
  PUBMED    32526136
REFERENCE   2  (bases 1 to 25018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..25018
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     2802..2823
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             4101..7784
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
                     DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
                     IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFRNRENMFS
                     EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
                     EFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
                     SDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
                     GPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
                     LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
                     LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
                     KDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
                     NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
                     IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
                     EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
                     EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
                     NTEVRVLLKHDDNPYVIGIARGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD
                     YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
                     FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ
                     NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
                     YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
                     QGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRN
                     YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS
                     VKH"
     CDS             7785..7832
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7839..7919
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             7926..7946
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7971..8120
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             8145..8165
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8595..8951
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             8970..9539
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     CDS             complement(10360..11175)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     11083..11102
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     11167..11186
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             17102..17962
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(17320..17339)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      18133..18721
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     18622..18641
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     18875..18892
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    19009..19030
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        19045..19075
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    19083..19099
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     19088..19110
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     19107..19123
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     19107..19123
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      19140..20482
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(20103..20150)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     20523..20541
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(20579..20601)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     20701..20720
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        20744..20959
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             20960..21763
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     primer_bind     complement(22045..22062)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(22045..22061)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(22054..22076)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(22361..22380)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      22402..2632
                     /label=AMA1
                     /note="replication origin from Aspergillus nidulans 
                     (Aleksenko and Clutterbuck, 1996)"
ORIGIN
        1 tgcagatttt gacctagtta acaaggcaca tcatgctatc acttccaaca ctcttgctga
       61 gcgcgttctg ccttgccttt ctggttctac gaagtatcca gtcccgcctg caacgccgca
      121 gaaacgccaa aaggtggggt tgtcaaccgc cagctagagc tccatcggga ttctttggca
      181 ttccgccgtt cctcggtggt cgcagataac tccctaaatt ccctgagcta actctaagtc
      241 gaccatgccg tttatggtta gcgcctccca aaaaggaatg gccgacttag agttacctct
      301 tgaccgactt tttctttcct cccccttaca tttcgttacc acaacacatt cctatatcca
      361 aactccaggt acataactag tcgaaatctc ttttaaatct agtcaagaac tagatactta
      421 accttcatac tgcttagaga tagcctttga agctcttatt tcgctctttg tctcactctc
      481 accttcctcc tcctcctcca atcctttttg ctcgggacta gtccaagact agtcaacgat
      541 tagtatgccg agcattcgcg ataacgatct ccgaaagtcc ccagagtact gtcactatct
      601 cgaggcagtt aaggacgggg agcttacgct gccggatttc aagatagtaa gccgacccga
      661 ctagctttaa gcctagttac tgactagtcc acaggacgac aatggcgtgc ctgatatcca
      721 tccatatgaa gtctactgcc gagtgaaggg atgcctcaag cgtacagtga gtctactgct
      781 atactagttt ctgactagtt attaactagt tgacaggttc cctctgccaa cagaaacata
      841 ttggtcaagc acttgaagga caagaactcc cacggcatgg agtttacatt gcacaatgga
      901 cctcccacta tgaaggaact gatggaggcc aaaggcaagt cctatctaga ttacttggtg
      961 actagtccct gactagtcaa gtagcatggt atgaaggctt gtttgaaggc actgttctcc
     1021 caaccccgac tcctaccaag aagcgcaagc gagctgcgta agtttctgtg agtctaacta
     1081 gtgtattagc taatatatag cagcaccaag tccaaggacc acaatactaa gggtgtcgag
     1141 aattcgtgag tttcttctcc catttcaact agtccttgac tagtcactaa ctactttgca
     1201 gcaacgaggg tgaagctgga aatgatcagg acaatggcga gggcccgtaa gtacagccat
     1261 tcaatgcaga ctagttgcta actaatctgt gactagttca agtggtccgt acgccgtgca
     1321 tacccctgtg actggtagga atttgagcaa gcctgtcttg ccgcgcgatg agaaaggaaa
     1381 ggcaagttac attcagcccc gtacctagga tcagtgctaa tttataacct ctagccactc
     1441 tttatgcaga tccgccgtga gggtagcaag gcagctaaat cagctggtga gaaaggaacc
     1501 ataccctgca agacctgtcg caacgcaaag ggcaaaggta agctatccaa gctagtttgg
     1561 gactagattc taactagtct cagcaccgtg tggttcaaag ccatattgcg agttttggcg
     1621 ctttttctca tcgattgacg aggcaaaggg agcgagtatg caacctcgta agtcagactc
     1681 agacaaagac caactagtta ttgaccagtc ttctagaagg ctctgttgtg gatcttgagg
     1741 ccctggagag ttcctccaac aatccggaga caagcaagtc ctcgtcggac tagtcactaa
     1801 ctagactcta actagttgca gacatggata atgcaaaaga gacaagcaat gaagaaagtg
     1861 gtaagacatt tctcctttgt ggttctggac tagtctttga ctagtcacag tcttaaacaa
     1921 ggaaaatgag catgaaaatg aggaggaaaa ggctgctgag cccgaggaag tgcagggtga
     1981 tggcagacat ggtaggttaa taccttgtta gttattgcta gtcactgact agtcaataac
     2041 tagtctctga acaccttgca atcactccgt ttgcgcagct gaacagtggt gaggataata
     2101 gtagtaagtt attctagctt cagagttata ggagactaga tactaactag tattagttgc
     2161 aactaacctg gatctcagag actttggcct caatctagaa tctatctagt tgtcaactag
     2221 actgtggtat cattgtcttt tattttccta gtcctggaac tagcttctaa ctagtctccc
     2281 taatatgtgg ctgtcttgtt tttttttttt gtttccctac ccggatatct agtccccttc
     2341 taggttctgt taacctctcg ggctctgatt tagtttaacg caaacctgag attagtttct
     2401 aactagtctc taggttttct atccaccttt aattgtaata ataaatacaa gcaacgttta
     2461 tacgtcaaaa gcatttataa acttttaccc taaagtagct tgcttgtgtg tttagtttat
     2521 aattagtctc ttattaattt gatgtaggta agcccgccac aaatatatat ttttaacaag
     2581 ataccgtgga aaaacttcgt gctatcacaa aacagtatac aaaaaataag ctggctttcc
     2641 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     2701 cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac
     2761 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     2821 tggaacaaca ctcaactcta tctcgggcta ttcttttgat ttataaggga ttttgccgat
     2881 ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     2941 aatggggtga cgatgagccg ctcttgcatc tttgtttgta ttatactgtc tttcttgtta
     3001 cacataatta ttctagaatg ccccaccgtt acatacggga cacagccatt tacatatgca
     3061 tgtggattac gagctaacga gttcattcaa atctcagaac tatcacataa tcatcattcc
     3121 cctatcgtca aagaccgtaa gacaaatccg gttcatgcac tgaacccatt cgggtagtga
     3181 gtcatttact cagcacactc gcgctgacgc tcgtcgaaca ccttcaatgc ctcctcggca
     3241 gccttgacac cactgagaac catggcaccg aaggtagggc ccatgcggtt aaagccatca
     3301 atttcagaca gctccatacc gccgattatc aagcccttag taacctcgcg ggtgttcttg
     3361 acgatggcat cctgccatgc ggagagacgg acggacgcag agagaagggc tgagtaataa
     3421 gcgccactgc gccagacagc tctggcggct ctgaggtgca gtggatgatt attaatccgg
     3481 gaccggccgc ccctccgccc cgaagtggaa aggctggtgt gcccctcgtt gaccaagaat
     3541 ctattgcatc atcggagaat atggagcttc atcgaatcac cggcagtaag cgaaggagaa
     3601 tgtgaagcca ggggtgtata gccgtcggcg aaatagcatg ccattaacct aggtacagaa
     3661 gtccaattgc ttccgatctg gtaaaagatt cacgagatag taccttctcc gaagtaggta
     3721 gagcgagtac ccggcgcgta agctccctaa ttggcccatc cggcatctgt agggcgtcca
     3781 aatatcgtgc ctctcctgct ttgcccggtg tatgaaaccg gaaaggccgc tcaggagctg
     3841 gccagcggcg cagaccggga acacaagctg gcagtcgacc catccggtgc tctgcactcg
     3901 acctgctgag gtccctcagt ccctggtagg cagctttgcc ccgtctgtcc gcccggtgtg
     3961 tcggcggggt tgacaaggtc gttgcgtcag tccaacattt gttgccatat tttcctgctc
     4021 tccccaccag ctgctctttt cttttctctt tcttttccca tcttcagtat attcatcttc
     4081 ccatccaaga acctttaatc atgagcaagc tggagaagtt tacaaactgc tactccctgt
     4141 ctaagaccct gaggttcaag gccatccctg tgggcaagac ccaggagaac atcgacaata
     4201 agcggctgct ggtggaggac gagaagagag ccgaggatta taagggcgtg aagaagctgc
     4261 tggatcgcta ctatctgtct tttatcaacg acgtgctgca cagcatcaag ctgaagaatc
     4321 tgaacaatta catcagcctg ttccggaaga aaaccagaac cgagaaggag aataaggagc
     4381 tggagaacct ggagatcaat ctgcggaagg agatcgccaa ggccttcaag ggcaacgagg
     4441 gctacaagtc cctgtttaag aaggatatca tcgagacaat cctgccagag ttcctggacg
     4501 ataaggacga gatcgccctg gtgaacagct tcaatggctt taccacagcc ttcaccggct
     4561 tctttcgtaa cagagagaat atgttttccg aggaggccaa gagcacatcc atcgccttca
     4621 ggtgtatcaa cgagaatctg acccgctaca tctctaatat ggacatcttc gagaaggtgg
     4681 acgccatctt tgataagcac gaggtgcagg agatcaagga gaagatcctg aacagcgact
     4741 atgatgtgga ggatttcttt gagggcgagt tctttaactt tgtgctgaca caggagggca
     4801 tcgacgtgta taacgccatc atcggcggct tcgtgaccga gagcggcgag aagatcaagg
     4861 gcctgaacga gtacatcaac ctgtataatc agaaaaccaa gcagaagctg cctaagttta
     4921 agccactgta taagcaggtg ctgagcgatc gggagtctct gagcttctac ggcgagggct
     4981 atacatccga tgaggaggtg ctggaggtgt ttagaaacac cctgaacaag aacagcgaga
     5041 tcttcagctc catcaagaag ctggagaagc tgttcaagaa ttttgacgag tactctagcg
     5101 ccggcatctt tgtgaagaac ggccccgcca tcagcacaat ctccaaggat atcttcggcg
     5161 agtggaacgt gatccgggac aagtggaatg ccgagtatga cgatatccac ctgaagaaga
     5221 aggccgtggt gaccgagaag tacgaggacg atcggagaaa gtccttcaag aagatcggct
     5281 ccttttctct ggagcagctg caggagtacg ccgacgccga tctgtctgtg gtggagaagc
     5341 tgaaggagat catcatccag aaggtggatg agatctacaa ggtgtatggc tcctctgaga
     5401 agctgttcga cgccgatttt gtgctggaga agagcctgaa gaagaacgac gccgtggtgg
     5461 ccatcatgaa ggacctgctg gattctgtga agagcttcga gaattacatc aaggccttct
     5521 ttggcgaggg caaggagaca aacagggacg agtccttcta tggcgatttt gtgctggcct
     5581 acgacatcct gctgaaggtg gaccacatct acgatgccat ccgcaattat gtgacccaga
     5641 agccctactc taaggataag ttcaagctgt attttcagaa ccctcagttc atgggcggct
     5701 gggacaagga taaggagaca gactatcggg ccaccatcct gagatacggc tccaagtact
     5761 atctggccat catggataag aagtacgcca agtgcctgca gaagatcgac aaggacgatg
     5821 tgaacggcaa ttacgagaag atcaactata agctgctgcc cggccctaat aagatgctgc
     5881 caaaggtgtt cttttctaag aagtggatgg cctactataa ccccagcgag gacatccaga
     5941 agatctacaa gaatggcaca ttcaagaagg gcgatatgtt taacctgaat gactgtcaca
     6001 agctgatcga cttctttaag gatagcatct cccggtatcc aaagtggtcc aatgcctacg
     6061 atttcaactt ttctgagaca gagaagtata aggacatcgc cggcttttac agagaggtgg
     6121 aggagcaggg ctataaggtg agcttcgagt ctgccagcaa gaaggaggtg gataagctgg
     6181 tggaggaggg caagctgtat atgttccaga tctataacaa ggacttttcc gataagtctc
     6241 acggcacacc caatctgcac accatgtact tcaagctgct gtttgacgag aacaatcacg
     6301 gacagatcag gctgagcgga ggagcagagc tgttcatgag gcgcgcctcc ctgaagaagg
     6361 aggagctggt ggtgcaccca gccaactccc ctatcgccaa caagaatcca gataatccca
     6421 agaaaaccac aaccctgtcc tacgacgtgt ataaggataa gaggttttct gaggaccagt
     6481 acgagctgca catcccaatc gccatcaata agtgccccaa gaacatcttc aagatcaata
     6541 cagaggtgcg cgtgctgctg aagcacgacg ataaccccta tgtgatcggc atcgctcgag
     6601 gcgagcgcaa tctgctgtat atcgtggtgg tggacggcaa gggcaacatc gtggagcagt
     6661 attccctgaa cgagatcatc aacaacttca acggcatcag gatcaagaca gattaccact
     6721 ctctgctgga caagaaggag aaggagaggt tcgaggcccg ccagaactgg acctccatcg
     6781 agaatatcaa ggagctgaag gccggctata tctctcaggt ggtgcacaag atctgcgagc
     6841 tggtggagaa gtacgatgcc gtgatcgccc tggaggacct gaactctggc tttaagaata
     6901 gccgcgtgaa ggtggagaag caggtgtatc agaagttcga gaagatgctg atcgataagc
     6961 tgaactacat ggtggacaag aagtctaatc cttgtgcaac aggcggcgcc ctgaagggct
     7021 atcagatcac caataagttc gagagcttta agtccatgtc tacccagaac ggcttcatct
     7081 tttacatccc tgcctggctg acatccaaga tcgatccatc taccggcttt gtgaacctgc
     7141 tgaaaaccaa gtataccagc atcgccgatt ccaagaagtt catcagctcc tttgacagga
     7201 tcatgtacgt gcccgaggag gatctgttcg agtttgccct ggactataag aacttctctc
     7261 gcacagacgc cgattacatc aagaagtgga agctgtactc ctacggcaac cggatcagaa
     7321 tcttccggaa tcctaagaag aacaacgtgt tcgactggga ggaggtgtgc ctgaccagcg
     7381 cctataagga gctgttcaac aagtacggca tcaattatca gcagggcgat atcagagccc
     7441 tgctgtgcga gcagtccgac aaggccttct actctagctt tatggccctg atgagcctga
     7501 tgctgcagat gcggaacagc atcacaggcc gcaccgacgt ggattttctg atcagccctg
     7561 tgaagaactc cgacggcatc ttctacgata gccggaacta tgaggcccag gagaatgcca
     7621 tcctgccaaa gaacgccgac gccaatggcg cctataacat cgccagaaag gtgctgtggg
     7681 ccatcggcca gttcaagaag gccgaggacg agaagctgga taaggtgaag atcgccatct
     7741 ctaacaagga gtggctggag tacgcccaga ccagcgtgaa gcacaaaagg ccggcggcca
     7801 cgaaaaaggc cggccaggca aaaaagaaaa agggatccta cccatacgat gttccagatt
     7861 acgcttatcc ctacgacgtg cctgattatg catacccata tgatgtcccc gactatgccg
     7921 ctgaccccaa gaagaagagg aaggtggagg ccagcggttc cggacgggct gacgcattgg
     7981 acgattttga tctggatatg ctgggaagtg acgccctcga tgattttgac cttgacatgc
     8041 ttggttcgga tgcccttgat gactttgacc tcgacatgct cggcagtgac gcccttgatg
     8101 atttcgacct ggacatgctg attaactcta gaagttccgg atctccgaaa aagaaacgca
     8161 aagttggtag ccagtacctg cccgacaccg acgaccggca ccggatcgag gaaaagcgga
     8221 agcggaccta cgagacattc aagagcatca tgaagaagtc ccccttcagc ggccccaccg
     8281 accctagacc tccacctaga agaatcgccg tgcccagcag atccagcgcc agcgtgccaa
     8341 aacctgcccc ccagccttac cccttcacca gcagcctgag caccatcaac tacgacgagt
     8401 tccctaccat ggtgttcccc agcggccaga tctctcaggc ctctgctctg gctccagccc
     8461 ctcctcaggt gctgcctcag gctcctgctc ctgcaccagc tccagccatg gtgtctgcac
     8521 tggctcaggc accagcaccc gtgcctgtgc tggctcctgg acctccacag gctgtggctc
     8581 caccagcccc taaacctaca caggccggcg agggcacact gtctgaagct ctgctgcagc
     8641 tgcagttcga cgacgaggat ctgggagccc tgctgggaaa cagcaccgat cctgccgtgt
     8701 tcaccgacct ggccagcgtg gacaacagcg agttccagca gctgctgaac cagggcatcc
     8761 ctgtggcccc tcacaccacc gagcccatgc tgatggaata ccccgaggcc atcacccggc
     8821 tcgtgacagg cgctcagagg cctcctgatc cagctcctgc ccctctggga gcaccaggcc
     8881 tgcctaatgg actgctgtct ggcgacgagg acttcagctc tatcgccgac atggacttct
     8941 ccgcactgct gggtagcgga tcgggatctc gggattccag ggaagggatg tttttgccga
     9001 agcctgaggc cggctccgct attagtgacg tgtttgaggg ccgcgaggtg tgccagccaa
     9061 aacgaatccg gccatttcat cctccaggaa gtccatgggc caaccgccca ctccccgcca
     9121 gcctcgcacc aacaccaacc ggtccagtac atgagccagt cgggtcactg accccggcac
     9181 cagtccctca gccactggat ccagcgcccg cagtgactcc cgaggccagt cacctgttgg
     9241 aggatcccga tgaagagacg agccaggctg tcaaagccct tcgggagatg gccgatactg
     9301 tgattcccca gaaggaagag gctgcaatct gtggccaaat ggacctttcc catccgcccc
     9361 caaggggcca tctggatgag ctgacaacca cacttgagtc catgaccgag gatctgaacc
     9421 tggactcacc cctgaccccg gaattgaacg agattctgga taccttcctg aacgacgagt
     9481 gcctcttgca tgccatgcat atcagcacag gactgtccat cttcgacaca tctctgtttt
     9541 agtgggcgac gacgacgttc gagggggggc ccggtaccgc cccgtccggt cctgcccgtc
     9601 accgagatcc acttaacgtt actgaaatca tcaaacagct tgacgaatct ggatataaga
     9661 tcgttggtgt cgatgtcagc tccggagttg agacaaatgg tgttcaggat ctcgataaga
     9721 tacgttcatt tgtccaagca gcaaagagtg ccttctagtg atttaatagc tccatgtcaa
     9781 caagaataaa acgcgtttcg ggtttacctc ttccagatac agctcatctg caatgcatgg
     9841 ccgccctaat acggcctgaa ttgattgtag acctttagac taaatcttct ttggctgcgc
     9901 taagtggcag agacttcaca ggtgtgagtc gagggtatac gcaccataca gaggcacggc
     9961 tggatgacaa ttaatggaga tagaagtcat gatagacagc gggctgactt ttcaattgac
    10021 agaatctgag tgtgcatata gtaggaccaa agaaaccgcc taaatccact tattcagagt
    10081 attcctcttc gatctggaga gcatacaatg taggtgatac ttcatggcat catcccgcac
    10141 gactctctcg cgggctcgcg ctccacgggg actcgcttca atttgttccg cggcctattg
    10201 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
    10261 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
    10321 taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat
    10381 caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg
    10441 tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta
    10501 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa
    10561 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa
    10621 aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa
    10681 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac
    10741 cctgtctgag ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac
    10801 tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc
    10861 tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg
    10921 cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt
    10981 aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt
    11041 cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata
    11101 cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg
    11161 ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt
    11221 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
    11281 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
    11341 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
    11401 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagtcgt
    11461 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
    11521 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
    11581 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggtttgtgc acacagccca
    11641 gcttggagcg aacgacctac accgaactga gatactaacc attatttttt tcctcaacat
    11701 aacgagaaca cacaggggcg ctatcgcaca gaatcaaatt cgatgactgg aaattttttg
    11761 ttaatttcag aggtcgcctg acgcatatac ctttttcaac tgaaaaattg ggagaaaaag
    11821 gaaaggtgag agcgccggaa ccggcttttc atatagaata gagaagcgtt catgactaaa
    11881 tgcttgcatc acaatacttg aagttgacaa tattatttaa ggacctattg tttttccaat
    11941 aggtggttag caatcgtctt actttctaac ttttcttacc ttttacattt cagcaatata
    12001 tatatatatt tcaaggatat accattctaa tgtctgcccc taagaagatc gtcgttttgc
    12061 caggtgacca cgttggtcaa gaaatcacag ccgaagccat taaggttctt aaagctattt
    12121 ctgatgttcg ttccaatgtc aagttcgatt tcgaaaatca tttaattggt ggtgctgcta
    12181 tcgaagctac aggtgtccca cttccagatg aggcgctgga agcctccaag aaggctgatg
    12241 ccgttttgtt aggtgctgtg ggtggtccta aatggggtac aggtagtgtt agacctgaac
    12301 aaggtttact aaaaatccgt aaagaacttc aattgtacgc caacttaaga ccatgtaact
    12361 ttgcatccga ctctctttta gacttatctc caatcaagcc acaatttgct aaaggtactg
    12421 acttcgttgt tgtcagagaa ttagtgggag gtatttactt tggtaagaga aaggaagatg
    12481 atggtgatgg tgtcgcttgg gatagtgaac aatacaccgt tccagaagtg caaagaatca
    12541 caagaatggc cgctttcatg gccctacaac atgagccacc attgcctatt tggtccttgg
    12601 ataaagctaa tgttttggcc tcttcaagat tatggagaaa aactgtggag gaaaccatca
    12661 agaacgaatt tcctacattg aaggttcaac atcaattgat tgattctgcc gccatgatcc
    12721 tagttaagaa cccaacccac ctaaatggta ttataatcac cagcaacatg tttggtgata
    12781 tcatctccga tgaagcctcc gttatcccag gttccttggg tttgttgcca tctgcgtcct
    12841 tggcctcttt gccagacaag aacaccgcat ttggtttgta cgaaccatgc cacggttctg
    12901 ctccagattt gccaaagaat aaggtcaacc ctatcgccac tatcttgtct gctgcaatga
    12961 tgttgaaatt gtcattgaac ttgcctgaag aaggtaaggc cattgaagat gcagttaaaa
    13021 aggttttgga tgcaggtatc agaactggtg atttaggtgg ttccaacagt accaccgaag
    13081 tcggtgatgc tgtcgccgaa gaagttaaga aaatcctcac ccctgcatct tggaatagct
    13141 tgcagtaggt ctaacagtgc tagagctgta ctattgtaag ttgaaacctt ggatacaacc
    13201 ttggctcgaa gagatgccaa aacgatcctt tctgctactg atgaatcacc ggcatagagt
    13261 attatatgtg cataaatatt gagcagagct gtggcaagtg gccttggctg gaatccgact
    13321 aaaaaaataa cacgcaaaaa aaaggaagac gggtgagggg ctgtttgagc cttgattcaa
    13381 agtcaaatca tcgcatttgg ctaccataat acctatactt cccagtctat ccagtctgtg
    13441 gtttatgatt gcttacaagt gctatccgta tcaggctagg aaaagtggaa gggaaatcgg
    13501 ttgaaccaag gccaaaatca ttgtaccatc ttgattgaaa actatataca aagatatcaa
    13561 gcatggacat cataaacatg aaacatctga cgattgtgtt tgtagttcca gcccgtggcc
    13621 gttgaaacgc ttattctggc atcttgcggc cgctggatga ctccctttcg atgataccct
    13681 ttagcagaag tgttcttaaa ttgcgggaag aggccgttca ggagtctggc tcgtttgatc
    13741 acatgggatt aaaatctggt gttgaggctg ggatatttta tgttttgtct cttgcgcttc
    13801 gatatcaaca gattgacaat aatgaataac tatctacgga ctatgaatgc agctcgatct
    13861 actccgtatt tagtcgggca atctcccgta gaagatgcta gactgcgctg aaagactagg
    13921 cacagttaat tgccgcggat cacgtcaggc ctgacaaatg acaggtggac cttcatgtaa
    13981 tcgcacgatg agtcagcgcc tcttctcccc cgccgacttg cagctgcgga tgtcccgagg
    14041 agccgaacat tgaatctatc ccaagagtga ctgaaatatg attgcaatcg tcgtcttttt
    14101 cttgcagaga atgtggtgag gtgatcaatc gtgtttttct tcatatatta tattctgtaa
    14161 tgatcatact ttgttgagat acccgtctct aaaattgtac tttcgcctcc ctcctccctc
    14221 tcaagtccgt catcgcaatg tcgaatttac cgtcccatgc ttctccgtct tttggggcgc
    14281 aaacgcctac cacgccgcag cccgtcgacg agctttctct caagtcgaac atcacatcag
    14341 cccctgcgtt acactccaga ttagacgaac tccgagatga gacttccagc cagatcagca
    14401 gcatagacac tgaccgcggg gacatgaccc ctgcggttcc cgcatcatta ctctccccat
    14461 ccttcactcc tccagcaacc cccgggggct cgatcaatac cgcggagctg cttcaacaga
    14521 cccaattagc tggcgcacgg caagggtcaa cacatactaa gcccccgagg ttgctttctc
    14581 gtcttcccaa tgtcgaatgt atcgtccgcg ctcggatacc cacgacaact ggcgcggaga
    14641 tgtttcttca cctgtaccac aatgacttag acaacaaaga gcatttggcg attgtgtttg
    14701 gaaatacaat tcgaagcaga agcttggaca agatccggcc tggagagacg gagatggatc
    14761 gcatgatccg tggcgcgtat gtcgggaaac tccgaccggg ccgtgtgagc agctggtatg
    14821 acgaggagaa agctgaagag gccgccagtc cggaagctgg cggcgctgta tccagtccgc
    14881 acacactgcc gcgaagtagc ctcaaggagg cacctcttgt gagaatccat tcggagtgct
    14941 acactggtga gacggcttgg tctgcacgct gcgactgcgg cgagcagctc gatgaagcag
    15001 ctcgcctcat gtcgctgcct atggaaacgt tggcagaggc tgcgtcaccg ccggatgggg
    15061 cggtaccgtc aaatgctgct ggaggtgtaa ttgtgtacct gcgccaagaa ggacgaggca
    15121 ttgggctggg cgagaaactg aaagcgtaca atcttcagga tctgggatca gacacggtgg
    15181 aagccaatct cctgcttcgc caccctgcgg atgcaagaag ttatggatta gctacggcga
    15241 tcctggtgga tcttggtcta gggattgact cgaatccgca cggcatccga ctactcacga
    15301 ataacccgga caagattcga gcggttgagg gacccaaccg agaagtggtg gtgaaagagc
    15361 gggtgccgat ggtgccattg gcatggagat cgggtggaaa gatgggaatc aagagttccg
    15421 aggtcgaagg gtatctgaga acgaaggtat gtcgacaatc tatctcttgc aagctagctt
    15481 attttctcat cattcttgaa caatgtctca ctctttcctc gcagatatcg aaaatgggcc
    15541 atttgctcca atgaacctat cccctttcgg ttagtaacct tgtgctggaa gtacgccttc
    15601 ctgcttttgg acttagccaa gtcgacacgg tgtatgctcg tcacactcat gtaacggttc
    15661 tgcagcgcaa acgttctggt cacgttattc gcttttgtcc attttgtctt tttgtgcggt
    15721 acataaacta gaggattagc gcactttgta catgatgttc ccgacatgtc acattaggcc
    15781 tcgattggtc aaaataatcc ctctctaacc tctggttcgc atactcttaa atgcagctct
    15841 agtctctaga cggactagtt cctcctgcag tggatgtctg acgagtgtct gcaaggaccg
    15901 ttttccgtgt ccagttagtt ccaatcaccc ggatatcggc gagacgtcgg ccgattgaga
    15961 tcactgagtc aatggcgttg agtggcaatt aatatatctg gatgcctaga atcctataaa
    16021 ttgggaaaac tagagacccc ggtctatcgt ggtggcagtg cgtcattttt tcctcttgga
    16081 gcaaaagtgt agtgccagta cgagtgttgt ggaggaaggc tgcatacatt gtgcctgtca
    16141 ttaaacgatg agctcgtccg tattggcccc tgtaatgcca tgttttccgc ccccaatcgt
    16201 caaggttttc cctttgttag attcctacca gtcatctagc aagtgaggta agctttgcca
    16261 gaaacgccaa ggctttatct atgtagtcga taagcaaagt ggactgatag cttaatatgg
    16321 aaggtccctc aggacaagtc gacctgtgca gaagagataa cagcttggca tcacgcatca
    16381 gtgcctcctc tcagacagaa taagtcgcca taagttatcg accgaaccta ggtagggtat
    16441 atttggtgac gacaatacct cccgacgata cctggattag acaccgtatg ccgcgaatta
    16501 acgccttccg accaagtccc ttttggtgat cgacacttta ggatcatctc ctgccagtcg
    16561 cccagactga cggtgttatg gtggcgcgct atggctttcc tagccggtca agctccgtgc
    16621 actagagagc ggagaaggag ggggagttga tagggaatga tatctaggta gagaagtact
    16681 ccaaccagag atatgcggat caatgctccg gcagttgtta gcatacccca acctcgttta
    16741 agtatcctct tcttccatcc atctttatca ttatttgtct atgtgcatcc ctagatcacg
    16801 tcaattattt cgataaatgg atacgtgcac tcgggaatgc gtgattggct cgtctcgtgt
    16861 tctcagttcc tcattccccc ccacgttaca ttgacaacgg agtattagcc tatggtcctg
    16921 ttcgataagc ttgctccatc gcctccacta cgtcaagcgg agtatcagac atgagctcta
    16981 ccccagatat cgcctgctta tcgctggttg ctttgtcatg caagtacaga tcacatcggc
    17041 aattctgata acagatggtg ccattgtccc gtaatcaatt gcccattgaa aaaggaagag
    17101 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
    17161 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
    17221 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
    17281 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
    17341 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
    17401 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
    17461 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
    17521 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
    17581 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
    17641 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
    17701 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
    17761 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
    17821 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
    17881 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
    17941 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
    18001 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
    18061 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
    18121 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
    18181 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
    18241 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
    18301 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
    18361 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
    18421 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
    18481 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
    18541 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
    18601 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
    18661 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
    18721 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
    18781 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
    18841 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
    18901 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
    18961 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
    19021 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
    19081 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccactga
    19141 acgaagcatc tgtgcttcat tttgtagaac aaaaatgcaa cgcgagagcg ctaatttttc
    19201 aaacaaagaa tctgagctgc atttttacag aacagaaatg caacgcgaaa gcgctatttt
    19261 accaacgaag aatctgtgct tcatttttgt aaaacaaaaa tgcaacgcga gagcgctaat
    19321 ttttcaaaca aagaatctga gctgcatttt tacagaacag aaatgcaacg cgagagcgct
    19381 attttaccaa caaagaatct atacttcttt tttgttctac aaaaatgcat cccgagagcg
    19441 ctatttttct aacaaagcat cttagattac tttttttctc ctttgtgcgc tctataatgc
    19501 agtctcttga taactttttg cactgtaggt ccgttaaggt tagaagaagg ctactttggt
    19561 gtctattttc tcttccataa aaaaagcctg actccacttc ccgcgtttac tgattactag
    19621 cgaagctgcg ggtgcatttt ttcaagataa aggcatcccc gattatattc tataccgatg
    19681 tggattgcgc atactttgtg aacagaaagt gatagcgttg atgattcttc attggtcaga
    19741 aaattatgaa cggtttcttc tattttgtct ctatatacta cgtataggaa atgtttacat
    19801 tttcgtattg ttttcgattc actctatgaa tagttcttac tacaattttt ttgtctaaag
    19861 agtaatacta gagataaaca taaaaaatgt agaggtcgag tttagatgca agttcaagga
    19921 gcgaaaggtg gatgggtagg ttatataggg atatagcaca gagatatata gcaaagagat
    19981 acttttgagc aatgtttgtg gaagcggtat tcgcaatatt ttagtagctc gttacagtcc
    20041 ggtgcgtttt tggttttttg aaagtgcgtc ttcagagcgc ttttggtttt caaaagcgct
    20101 ctgaagttcc tatactttct agagaatagg aacttcggaa taggaacttc aaagcgtttc
    20161 cgaaaacgag cgcttccgaa aatgcaacgc gagctgcgca catacagctc actgttcacg
    20221 tcgcacctat atctgcgtgt tgcctgtata tatatataca tgagaagaac ggcatagtgc
    20281 gtgtttatgc ttaaatgcgt acttatatgc gtctatttat gtaggatgaa aggtagtcta
    20341 gtacctcctg tgatattatc ccattccatg cggggtatcg tatgcttcct tcagcactac
    20401 cctttagctg ttctatatgc tgccactcct caattggatt agtctcatcc ttcaatgcta
    20461 tcatttcctt tgatattgga tcatactaag aaaccattat tatcatgaca ttaacctata
    20521 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
    20581 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
    20641 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
    20701 cggcatcaga gcagattgta ctgagagtgc accataccac cttttcaatt catcattttt
    20761 tttttattct tttttttgat ttcggtttcc ttgaaatttt tttgattcgg taatctccga
    20821 acagaaggaa gaacgaagga aggagcacag acttagattg gtatatatac gcatatgtag
    20881 tgttgaagaa acatgaaatt gcccagtatt cttaacccaa ctgcacagaa caaaaacctg
    20941 caggaaacga agataaatca tgtcgaaagc tacatataag gaacgtgctg ctactcatcc
    21001 tagtcctgtt gctgccaagc tatttaatat catgcacgaa aagcaaacaa acttgtgtgc
    21061 ttcattggat gttcgtacca ccaaggaatt actggagtta gttgaagcat taggtcccaa
    21121 aatttgttta ctaaaaacac atgtggatat cttgactgat ttttccatgg agggcacagt
    21181 taagccgcta aaggcattat ccgccaagta caatttttta ctcttcgaag acagaaaatt
    21241 tgctgacatt ggtaatacag tcaaattgca gtactctgcg ggtgtataca gaatagcaga
    21301 atgggcagac attacgaatg cacacggtgt ggtgggccca ggtattgtta gcggtttgaa
    21361 gcaggcggca gaagaagtaa caaaggaacc tagaggcctt ttgatgttag cagaattgtc
    21421 atgcaagggc tccctatcta ctggagaata tactaagggt actgttgaca ttgcgaagag
    21481 cgacaaagat tttgttatcg gctttattgc tcaaagagac atgggtggaa gagatgaagg
    21541 ttacgattgg ttgattatga cacccggtgt gggtttagat gacaagggag acgcattggg
    21601 tcaacagtat agaaccgtgg atgatgtggt ctctacagga tctgacatta ttattgttgg
    21661 aagaggacta tttgcaaagg gaagggatgc taaggtagag ggtgaacgtt acagaaaagc
    21721 aggctgggaa gcatatttga gaagatgcgg ccagcaaaac taaaaaactg tattataagt
    21781 aaatgcatgt atactaaact cacaaattag agcttcaatt taattatatc agttattacc
    21841 ctatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggaaattgt
    21901 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
    21961 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
    22021 gagtggggta ccgagctcga attcactggc cgtcgtttta caacgtcgtg actgggaaaa
    22081 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
    22141 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
    22201 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca
    22261 aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
    22321 cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct
    22381 tcctttctcg ccacgttcgc cagcttattt tttgtatact gttttgtgat agcacgaagt
    22441 ttttccacgg tatcttgtta aaaatatata tttgtggcgg gcttacctac atcaaattaa
    22501 taagagacta attataaact aaacacacaa gcaagctact ttagggtaaa agtttataaa
    22561 tgcttttgac gtataaacgt tgcttgtatt tattattaca attaaaggtg gatagaaaac
    22621 ctagagacta gttagaaact aatctcaggt ttgcgttaaa ctaaatcaga gcccgagagg
    22681 ttaacagaac ctagaagggg actagatatc cgggtaggga aacaaaaaaa aaaaacaaga
    22741 cagccacata ttagggagac tagttagaag ctagttccag gactaggaaa ataaaagaca
    22801 atgataccac agtctagttg acaactagat agattctaga ttgaggccaa agtctctgag
    22861 atccaggtta gttgcaacta atactagtta gtatctagtc tcctataact ctgaagctag
    22921 aataacttac tactattatc ctcaccactg ttcagctgcg caaacggagt gattgcaagg
    22981 tgttcagaga ctagttattg actagtcagt gactagcaat aactaacaag gtattaacct
    23041 accatgtctg ccatcaccct gcacttcctc gggctcagca gccttttcct cctcattttc
    23101 atgctcattt tccttgttta agactgtgac tagtcaaaga ctagtccaga accacaaagg
    23161 agaaatgtct taccactttc ttcattgctt gtctcttttg cattatccat gtctgcaact
    23221 agttagagtc tagttagtga ctagtccgac gaggacttgc ttgtctccgg attgttggag
    23281 gaactctcca gggcctcaag atccacaaca gagccttcta gaagactggt caataactag
    23341 ttggtctttg tctgagtctg acttacgagg ttgcatactc gctccctttg cctcgtcaat
    23401 cgatgagaaa aagcgccaaa actcgcaata tggctttgaa ccacacggtg ctgagactag
    23461 ttagaatcta gtcccaaact agcttggata gcttaccttt gccctttgcg ttgcgacagg
    23521 tcttgcaggg tatggttcct ttctcaccag ctgatttagc tgccttgcta ccctcacggc
    23581 ggatctgcat aaagagtggc tagaggttat aaattagcac tgatcctagg tacggggctg
    23641 aatgtaactt gcctttcctt tctcatcgcg cggcaagaca ggcttgctca aattcctacc
    23701 agtcacaggg gtatgcacgg cgtacggacc acttgaacta gtcacagatt agttagcaac
    23761 tagtctgcat tgaatggctg tacttacggg ccctcgccat tgtcctgatc atttccagct
    23821 tcaccctcgt tgctgcaaag tagttagtga ctagtcaagg actagttgaa atgggagaag
    23881 aaactcacga attctcgaca cccttagtat tgtggtcctt ggacttggtg ctgctatata
    23941 ttagctaata cactagttag actcacagaa acttacgcag ctcgcttgcg cttcttggta
    24001 ggagtcgggg ttgggagaac agtgccttca aacaagcctt cataccatgc tacttgacta
    24061 gtcagggact agtcaccaag taatctagat aggacttgcc tttggcctcc atcagttcct
    24121 tcatagtggg aggtccattg tgcaatgtaa actccatgcc gtgggagttc ttgtccttca
    24181 agtgcttgac caatatgttt ctgttggcag agggaacctg tcaactagtt aataactagt
    24241 cagaaactag tatagcagta gactcactgt acgcttgagg catcccttca ctcggcagta
    24301 gacttcatat ggatggatat caggcacgcc attgtcgtcc tgtggactag tcagtaacta
    24361 ggcttaaagc tagtcgggtc ggcttactat cttgaaatcc ggcagcgtaa gctccccgtc
    24421 cttaactgcc tcgagatagt gacagtactc tggggacttt cggagatcgt tatcgcgaat
    24481 gctcggcata ctaatcgttg actagtcttg gactagtccc gagcaaaaag gattggagga
    24541 ggaggaggaa ggtgagagtg agacaaagag cgaaataaga gcttcaaagg ctatctctaa
    24601 gcagtatgaa ggttaagtat ctagttcttg actagattta aaagagattt cgactagtta
    24661 tgtacctgga gtttggatat aggaatgtgt tgtggtaacg aaatgtaagg gggaggaaag
    24721 aaaaagtcgg tcaagaggta actctaagtc ggccattcct ttttgggagg cgctaaccat
    24781 aaacggcatg gtcgacttag agttagctca gggaatttag ggagttatct gcgaccactg
    24841 gactctcagg ctttccattg gggtactaac atagccatca aatgccggcc cgtgtttaat
    24901 ctcctcgaga tggctgggct cacatgcgtt ctactcaaaa actcttatac ctaggccctc
    24961 tcccaatcgt gtatgtttgg accttcctct ctcacacttt ggataacatc aagcatac
//