| 质粒名称: | pCRI005-pYFAC-riboB-PgpdA-dLbCpf1(D156R)-VPR-TtrpC |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 140198 |
| 存储实验室: | Yit Heng Chooi |
|
载体骨架基本信息 |
|
| 载体名称: | pYFAC-riboB |
| 载体出品公司: | DOI: 10.1039/C8SC02870B |
| 空载体大小: | 14777 bp |
| 完整质粒大小: | 25045 bp |
| 载体修饰: | 我们添加了Leu-Kan标记，以便在克隆过程中进行更严格的选择。 |
| 载体类型: | CRISPR，合成生物学；在巢状曲霉和相关丝状真菌中的表达。 |
| 筛选标记: | LEU2, URA3 ; Af riboB |
| 载体抗性: | 氨苄霉素和卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 低拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | dLbCas12a(D156R)-VPR,dCpf1(D156R)-VPR |
| 物种来源: | 合成的紫胶螺科细菌 |
| 插入大小: | 5349 bp |
| 基因突变: | D832A脱氧核糖核酸酶失活，D156R用于在25℃下提高活性 |
| 启动子: | PgpdA |
| 标签/融合蛋白: | VPR (VP64-p65-Rta), NLS |
| 克隆方法: | Gibson 克隆 |
| 5’测序引物 | CACTGAGAACCATGGCACCGAAG |
|
文献引用方法 |
|
| 材料和方法部分: | pCRI005-pYFAC-riboB-PgpdA-dLbCpf1(D156R)-VPR-TtrpC was a gift from Yit Heng Chooi (Addgene plasmid # 140198 ; http://n2t.net/addgene:140198 ; RRID:Addgene_140198) |
| 参考文献部分: | CRISPR-mediated activation of biosynthetic gene clusters for bioactive molecule discovery in filamentous fungi. Roux I, Woodcraft C, Hu J, Wolters R, Gilchrist CLM, Chooi YH. ACS Synth Biol. 2020 Jun 11. doi: 10.1021/acssynbio.0c00197. 10.1021/acssynbio.0c00197 PubMed 32526136 |
pCRI005-pYFAC-riboB-PgpdA-dLbCpf1(D156R)-VPR-TtrpC
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
-VPR-TtrpC载体图谱.png)
载体简介
载体序列
LOCUS pCRI005-pYFAC-riboB-PgpdA-dLbCpf1(D156R)-VPR-TtrpC 25018 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Episomal expression of dLbCpf1(D156R)-VPR, variant for culture at
room temperature. Filamentous fungi vector with AMA1 and riboB
selection marker..
ACCESSION .
VERSION .
KEYWORDS pCRI005-pYFAC-riboB-PgpdA-dLbCpf1(D156R)-VPR-TtrpC
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 25018)
AUTHORS Roux I, Woodcraft C, Hu J, Wolters R, Gilchrist CLM, Chooi YH
TITLE CRISPR-mediated activation of biosynthetic gene clusters for
bioactive molecule discovery in filamentous fungi.
JOURNAL ACS Synth Biol. 2020 Jun 11. doi: 10.1021/acssynbio.0c00197.
PUBMED 32526136
REFERENCE 2 (bases 1 to 25018)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..25018
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 2802..2823
/label=F1ori-F
/note="F1 origin, forward primer"
CDS 4101..7784
/codon_start=1
/product="CRISPR-associated protein from Lachnospiraceae
bacterium ND2006 (Zetsche et al., 2015)"
/label=LbCpf1
/translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFRNRENMFS
EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
EFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
SDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
GPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
KDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
NTEVRVLLKHDDNPYVIGIARGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD
YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ
NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
QGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRN
YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS
VKH"
CDS 7785..7832
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=nucleoplasmin NLS
/translation="KRPAATKKAGQAKKKK"
CDS 7839..7919
/codon_start=1
/product="three tandem HA epitope tags"
/label=3xHA
/translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
CDS 7926..7946
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 7971..8120
/codon_start=1
/product="tetrameric repeat of the minimal activation
domain of herpes simplex virus VP16 (Beerli et al., 1998)"
/label=VP64
/translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
DLDML"
CDS 8145..8165
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 8595..8951
/codon_start=1
/product="transcriptional activation domain of human RelA,
also known as p65 (O'Shea and Perkins, 2008)"
/label=RelA (p65) AD
/translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
EDFSSIADMDFSALL"
CDS 8970..9539
/codon_start=1
/product="transcriptional activation domain from the human
herpesvirus 4 (Epstein-Barr virus) replication and
transcription activator Rta/BRLF1 (Hardwick et al., 1992;
Chavez et al., 2015)"
/label=Rta AD
/translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
DTFLNDECLLHAMHISTGLSIFDTSLF"
CDS complement(10360..11175)
/codon_start=1
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
primer_bind 11083..11102
/label=Kan-R
/note="Kanamycin resistance gene, reverse primer"
primer_bind 11167..11186
/label=pENTR-R
/note="pENTR vectors, reverse primer"
CDS 17102..17962
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(17320..17339)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 18133..18721
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 18622..18641
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 18875..18892
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 19009..19030
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 19045..19075
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 19083..19099
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 19088..19110
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 19107..19123
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 19107..19123
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
rep_origin 19140..20482
/label=2u ori
/note="yeast 2u plasmid origin of replication"
protein_bind complement(20103..20150)
/label=FRT
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="FLP-mediated recombination occurs in the 8-bp core
sequence TCTAGAAA (Turan and Bode, 2011)."
primer_bind 20523..20541
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(20579..20601)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 20701..20720
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
promoter 20744..20959
/gene="S. cerevisiae URA3"
/label=URA3 promoter
CDS 20960..21763
/codon_start=1
/gene="S. cerevisiae URA3"
/product="orotidine-5'-phosphate decarboxylase, required
for uracil biosynthesis"
/label=URA3
/note="yeast auxotrophic marker, counterselectable with
5-fluoroorotic acid (5-FOA)"
/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
primer_bind complement(22045..22062)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(22045..22061)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(22054..22076)
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind complement(22361..22380)
/label=F1ori-R
/note="F1 origin, reverse primer"
rep_origin 22402..2632
/label=AMA1
/note="replication origin from Aspergillus nidulans
(Aleksenko and Clutterbuck, 1996)"
ORIGIN
1 tgcagatttt gacctagtta acaaggcaca tcatgctatc acttccaaca ctcttgctga
61 gcgcgttctg ccttgccttt ctggttctac gaagtatcca gtcccgcctg caacgccgca
121 gaaacgccaa aaggtggggt tgtcaaccgc cagctagagc tccatcggga ttctttggca
181 ttccgccgtt cctcggtggt cgcagataac tccctaaatt ccctgagcta actctaagtc
241 gaccatgccg tttatggtta gcgcctccca aaaaggaatg gccgacttag agttacctct
301 tgaccgactt tttctttcct cccccttaca tttcgttacc acaacacatt cctatatcca
361 aactccaggt acataactag tcgaaatctc ttttaaatct agtcaagaac tagatactta
421 accttcatac tgcttagaga tagcctttga agctcttatt tcgctctttg tctcactctc
481 accttcctcc tcctcctcca atcctttttg ctcgggacta gtccaagact agtcaacgat
541 tagtatgccg agcattcgcg ataacgatct ccgaaagtcc ccagagtact gtcactatct
601 cgaggcagtt aaggacgggg agcttacgct gccggatttc aagatagtaa gccgacccga
661 ctagctttaa gcctagttac tgactagtcc acaggacgac aatggcgtgc ctgatatcca
721 tccatatgaa gtctactgcc gagtgaaggg atgcctcaag cgtacagtga gtctactgct
781 atactagttt ctgactagtt attaactagt tgacaggttc cctctgccaa cagaaacata
841 ttggtcaagc acttgaagga caagaactcc cacggcatgg agtttacatt gcacaatgga
901 cctcccacta tgaaggaact gatggaggcc aaaggcaagt cctatctaga ttacttggtg
961 actagtccct gactagtcaa gtagcatggt atgaaggctt gtttgaaggc actgttctcc
1021 caaccccgac tcctaccaag aagcgcaagc gagctgcgta agtttctgtg agtctaacta
1081 gtgtattagc taatatatag cagcaccaag tccaaggacc acaatactaa gggtgtcgag
1141 aattcgtgag tttcttctcc catttcaact agtccttgac tagtcactaa ctactttgca
1201 gcaacgaggg tgaagctgga aatgatcagg acaatggcga gggcccgtaa gtacagccat
1261 tcaatgcaga ctagttgcta actaatctgt gactagttca agtggtccgt acgccgtgca
1321 tacccctgtg actggtagga atttgagcaa gcctgtcttg ccgcgcgatg agaaaggaaa
1381 ggcaagttac attcagcccc gtacctagga tcagtgctaa tttataacct ctagccactc
1441 tttatgcaga tccgccgtga gggtagcaag gcagctaaat cagctggtga gaaaggaacc
1501 ataccctgca agacctgtcg caacgcaaag ggcaaaggta agctatccaa gctagtttgg
1561 gactagattc taactagtct cagcaccgtg tggttcaaag ccatattgcg agttttggcg
1621 ctttttctca tcgattgacg aggcaaaggg agcgagtatg caacctcgta agtcagactc
1681 agacaaagac caactagtta ttgaccagtc ttctagaagg ctctgttgtg gatcttgagg
1741 ccctggagag ttcctccaac aatccggaga caagcaagtc ctcgtcggac tagtcactaa
1801 ctagactcta actagttgca gacatggata atgcaaaaga gacaagcaat gaagaaagtg
1861 gtaagacatt tctcctttgt ggttctggac tagtctttga ctagtcacag tcttaaacaa
1921 ggaaaatgag catgaaaatg aggaggaaaa ggctgctgag cccgaggaag tgcagggtga
1981 tggcagacat ggtaggttaa taccttgtta gttattgcta gtcactgact agtcaataac
2041 tagtctctga acaccttgca atcactccgt ttgcgcagct gaacagtggt gaggataata
2101 gtagtaagtt attctagctt cagagttata ggagactaga tactaactag tattagttgc
2161 aactaacctg gatctcagag actttggcct caatctagaa tctatctagt tgtcaactag
2221 actgtggtat cattgtcttt tattttccta gtcctggaac tagcttctaa ctagtctccc
2281 taatatgtgg ctgtcttgtt tttttttttt gtttccctac ccggatatct agtccccttc
2341 taggttctgt taacctctcg ggctctgatt tagtttaacg caaacctgag attagtttct
2401 aactagtctc taggttttct atccaccttt aattgtaata ataaatacaa gcaacgttta
2461 tacgtcaaaa gcatttataa acttttaccc taaagtagct tgcttgtgtg tttagtttat
2521 aattagtctc ttattaattt gatgtaggta agcccgccac aaatatatat ttttaacaag
2581 ataccgtgga aaaacttcgt gctatcacaa aacagtatac aaaaaataag ctggctttcc
2641 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
2701 cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac
2761 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
2821 tggaacaaca ctcaactcta tctcgggcta ttcttttgat ttataaggga ttttgccgat
2881 ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
2941 aatggggtga cgatgagccg ctcttgcatc tttgtttgta ttatactgtc tttcttgtta
3001 cacataatta ttctagaatg ccccaccgtt acatacggga cacagccatt tacatatgca
3061 tgtggattac gagctaacga gttcattcaa atctcagaac tatcacataa tcatcattcc
3121 cctatcgtca aagaccgtaa gacaaatccg gttcatgcac tgaacccatt cgggtagtga
3181 gtcatttact cagcacactc gcgctgacgc tcgtcgaaca ccttcaatgc ctcctcggca
3241 gccttgacac cactgagaac catggcaccg aaggtagggc ccatgcggtt aaagccatca
3301 atttcagaca gctccatacc gccgattatc aagcccttag taacctcgcg ggtgttcttg
3361 acgatggcat cctgccatgc ggagagacgg acggacgcag agagaagggc tgagtaataa
3421 gcgccactgc gccagacagc tctggcggct ctgaggtgca gtggatgatt attaatccgg
3481 gaccggccgc ccctccgccc cgaagtggaa aggctggtgt gcccctcgtt gaccaagaat
3541 ctattgcatc atcggagaat atggagcttc atcgaatcac cggcagtaag cgaaggagaa
3601 tgtgaagcca ggggtgtata gccgtcggcg aaatagcatg ccattaacct aggtacagaa
3661 gtccaattgc ttccgatctg gtaaaagatt cacgagatag taccttctcc gaagtaggta
3721 gagcgagtac ccggcgcgta agctccctaa ttggcccatc cggcatctgt agggcgtcca
3781 aatatcgtgc ctctcctgct ttgcccggtg tatgaaaccg gaaaggccgc tcaggagctg
3841 gccagcggcg cagaccggga acacaagctg gcagtcgacc catccggtgc tctgcactcg
3901 acctgctgag gtccctcagt ccctggtagg cagctttgcc ccgtctgtcc gcccggtgtg
3961 tcggcggggt tgacaaggtc gttgcgtcag tccaacattt gttgccatat tttcctgctc
4021 tccccaccag ctgctctttt cttttctctt tcttttccca tcttcagtat attcatcttc
4081 ccatccaaga acctttaatc atgagcaagc tggagaagtt tacaaactgc tactccctgt
4141 ctaagaccct gaggttcaag gccatccctg tgggcaagac ccaggagaac atcgacaata
4201 agcggctgct ggtggaggac gagaagagag ccgaggatta taagggcgtg aagaagctgc
4261 tggatcgcta ctatctgtct tttatcaacg acgtgctgca cagcatcaag ctgaagaatc
4321 tgaacaatta catcagcctg ttccggaaga aaaccagaac cgagaaggag aataaggagc
4381 tggagaacct ggagatcaat ctgcggaagg agatcgccaa ggccttcaag ggcaacgagg
4441 gctacaagtc cctgtttaag aaggatatca tcgagacaat cctgccagag ttcctggacg
4501 ataaggacga gatcgccctg gtgaacagct tcaatggctt taccacagcc ttcaccggct
4561 tctttcgtaa cagagagaat atgttttccg aggaggccaa gagcacatcc atcgccttca
4621 ggtgtatcaa cgagaatctg acccgctaca tctctaatat ggacatcttc gagaaggtgg
4681 acgccatctt tgataagcac gaggtgcagg agatcaagga gaagatcctg aacagcgact
4741 atgatgtgga ggatttcttt gagggcgagt tctttaactt tgtgctgaca caggagggca
4801 tcgacgtgta taacgccatc atcggcggct tcgtgaccga gagcggcgag aagatcaagg
4861 gcctgaacga gtacatcaac ctgtataatc agaaaaccaa gcagaagctg cctaagttta
4921 agccactgta taagcaggtg ctgagcgatc gggagtctct gagcttctac ggcgagggct
4981 atacatccga tgaggaggtg ctggaggtgt ttagaaacac cctgaacaag aacagcgaga
5041 tcttcagctc catcaagaag ctggagaagc tgttcaagaa ttttgacgag tactctagcg
5101 ccggcatctt tgtgaagaac ggccccgcca tcagcacaat ctccaaggat atcttcggcg
5161 agtggaacgt gatccgggac aagtggaatg ccgagtatga cgatatccac ctgaagaaga
5221 aggccgtggt gaccgagaag tacgaggacg atcggagaaa gtccttcaag aagatcggct
5281 ccttttctct ggagcagctg caggagtacg ccgacgccga tctgtctgtg gtggagaagc
5341 tgaaggagat catcatccag aaggtggatg agatctacaa ggtgtatggc tcctctgaga
5401 agctgttcga cgccgatttt gtgctggaga agagcctgaa gaagaacgac gccgtggtgg
5461 ccatcatgaa ggacctgctg gattctgtga agagcttcga gaattacatc aaggccttct
5521 ttggcgaggg caaggagaca aacagggacg agtccttcta tggcgatttt gtgctggcct
5581 acgacatcct gctgaaggtg gaccacatct acgatgccat ccgcaattat gtgacccaga
5641 agccctactc taaggataag ttcaagctgt attttcagaa ccctcagttc atgggcggct
5701 gggacaagga taaggagaca gactatcggg ccaccatcct gagatacggc tccaagtact
5761 atctggccat catggataag aagtacgcca agtgcctgca gaagatcgac aaggacgatg
5821 tgaacggcaa ttacgagaag atcaactata agctgctgcc cggccctaat aagatgctgc
5881 caaaggtgtt cttttctaag aagtggatgg cctactataa ccccagcgag gacatccaga
5941 agatctacaa gaatggcaca ttcaagaagg gcgatatgtt taacctgaat gactgtcaca
6001 agctgatcga cttctttaag gatagcatct cccggtatcc aaagtggtcc aatgcctacg
6061 atttcaactt ttctgagaca gagaagtata aggacatcgc cggcttttac agagaggtgg
6121 aggagcaggg ctataaggtg agcttcgagt ctgccagcaa gaaggaggtg gataagctgg
6181 tggaggaggg caagctgtat atgttccaga tctataacaa ggacttttcc gataagtctc
6241 acggcacacc caatctgcac accatgtact tcaagctgct gtttgacgag aacaatcacg
6301 gacagatcag gctgagcgga ggagcagagc tgttcatgag gcgcgcctcc ctgaagaagg
6361 aggagctggt ggtgcaccca gccaactccc ctatcgccaa caagaatcca gataatccca
6421 agaaaaccac aaccctgtcc tacgacgtgt ataaggataa gaggttttct gaggaccagt
6481 acgagctgca catcccaatc gccatcaata agtgccccaa gaacatcttc aagatcaata
6541 cagaggtgcg cgtgctgctg aagcacgacg ataaccccta tgtgatcggc atcgctcgag
6601 gcgagcgcaa tctgctgtat atcgtggtgg tggacggcaa gggcaacatc gtggagcagt
6661 attccctgaa cgagatcatc aacaacttca acggcatcag gatcaagaca gattaccact
6721 ctctgctgga caagaaggag aaggagaggt tcgaggcccg ccagaactgg acctccatcg
6781 agaatatcaa ggagctgaag gccggctata tctctcaggt ggtgcacaag atctgcgagc
6841 tggtggagaa gtacgatgcc gtgatcgccc tggaggacct gaactctggc tttaagaata
6901 gccgcgtgaa ggtggagaag caggtgtatc agaagttcga gaagatgctg atcgataagc
6961 tgaactacat ggtggacaag aagtctaatc cttgtgcaac aggcggcgcc ctgaagggct
7021 atcagatcac caataagttc gagagcttta agtccatgtc tacccagaac ggcttcatct
7081 tttacatccc tgcctggctg acatccaaga tcgatccatc taccggcttt gtgaacctgc
7141 tgaaaaccaa gtataccagc atcgccgatt ccaagaagtt catcagctcc tttgacagga
7201 tcatgtacgt gcccgaggag gatctgttcg agtttgccct ggactataag aacttctctc
7261 gcacagacgc cgattacatc aagaagtgga agctgtactc ctacggcaac cggatcagaa
7321 tcttccggaa tcctaagaag aacaacgtgt tcgactggga ggaggtgtgc ctgaccagcg
7381 cctataagga gctgttcaac aagtacggca tcaattatca gcagggcgat atcagagccc
7441 tgctgtgcga gcagtccgac aaggccttct actctagctt tatggccctg atgagcctga
7501 tgctgcagat gcggaacagc atcacaggcc gcaccgacgt ggattttctg atcagccctg
7561 tgaagaactc cgacggcatc ttctacgata gccggaacta tgaggcccag gagaatgcca
7621 tcctgccaaa gaacgccgac gccaatggcg cctataacat cgccagaaag gtgctgtggg
7681 ccatcggcca gttcaagaag gccgaggacg agaagctgga taaggtgaag atcgccatct
7741 ctaacaagga gtggctggag tacgcccaga ccagcgtgaa gcacaaaagg ccggcggcca
7801 cgaaaaaggc cggccaggca aaaaagaaaa agggatccta cccatacgat gttccagatt
7861 acgcttatcc ctacgacgtg cctgattatg catacccata tgatgtcccc gactatgccg
7921 ctgaccccaa gaagaagagg aaggtggagg ccagcggttc cggacgggct gacgcattgg
7981 acgattttga tctggatatg ctgggaagtg acgccctcga tgattttgac cttgacatgc
8041 ttggttcgga tgcccttgat gactttgacc tcgacatgct cggcagtgac gcccttgatg
8101 atttcgacct ggacatgctg attaactcta gaagttccgg atctccgaaa aagaaacgca
8161 aagttggtag ccagtacctg cccgacaccg acgaccggca ccggatcgag gaaaagcgga
8221 agcggaccta cgagacattc aagagcatca tgaagaagtc ccccttcagc ggccccaccg
8281 accctagacc tccacctaga agaatcgccg tgcccagcag atccagcgcc agcgtgccaa
8341 aacctgcccc ccagccttac cccttcacca gcagcctgag caccatcaac tacgacgagt
8401 tccctaccat ggtgttcccc agcggccaga tctctcaggc ctctgctctg gctccagccc
8461 ctcctcaggt gctgcctcag gctcctgctc ctgcaccagc tccagccatg gtgtctgcac
8521 tggctcaggc accagcaccc gtgcctgtgc tggctcctgg acctccacag gctgtggctc
8581 caccagcccc taaacctaca caggccggcg agggcacact gtctgaagct ctgctgcagc
8641 tgcagttcga cgacgaggat ctgggagccc tgctgggaaa cagcaccgat cctgccgtgt
8701 tcaccgacct ggccagcgtg gacaacagcg agttccagca gctgctgaac cagggcatcc
8761 ctgtggcccc tcacaccacc gagcccatgc tgatggaata ccccgaggcc atcacccggc
8821 tcgtgacagg cgctcagagg cctcctgatc cagctcctgc ccctctggga gcaccaggcc
8881 tgcctaatgg actgctgtct ggcgacgagg acttcagctc tatcgccgac atggacttct
8941 ccgcactgct gggtagcgga tcgggatctc gggattccag ggaagggatg tttttgccga
9001 agcctgaggc cggctccgct attagtgacg tgtttgaggg ccgcgaggtg tgccagccaa
9061 aacgaatccg gccatttcat cctccaggaa gtccatgggc caaccgccca ctccccgcca
9121 gcctcgcacc aacaccaacc ggtccagtac atgagccagt cgggtcactg accccggcac
9181 cagtccctca gccactggat ccagcgcccg cagtgactcc cgaggccagt cacctgttgg
9241 aggatcccga tgaagagacg agccaggctg tcaaagccct tcgggagatg gccgatactg
9301 tgattcccca gaaggaagag gctgcaatct gtggccaaat ggacctttcc catccgcccc
9361 caaggggcca tctggatgag ctgacaacca cacttgagtc catgaccgag gatctgaacc
9421 tggactcacc cctgaccccg gaattgaacg agattctgga taccttcctg aacgacgagt
9481 gcctcttgca tgccatgcat atcagcacag gactgtccat cttcgacaca tctctgtttt
9541 agtgggcgac gacgacgttc gagggggggc ccggtaccgc cccgtccggt cctgcccgtc
9601 accgagatcc acttaacgtt actgaaatca tcaaacagct tgacgaatct ggatataaga
9661 tcgttggtgt cgatgtcagc tccggagttg agacaaatgg tgttcaggat ctcgataaga
9721 tacgttcatt tgtccaagca gcaaagagtg ccttctagtg atttaatagc tccatgtcaa
9781 caagaataaa acgcgtttcg ggtttacctc ttccagatac agctcatctg caatgcatgg
9841 ccgccctaat acggcctgaa ttgattgtag acctttagac taaatcttct ttggctgcgc
9901 taagtggcag agacttcaca ggtgtgagtc gagggtatac gcaccataca gaggcacggc
9961 tggatgacaa ttaatggaga tagaagtcat gatagacagc gggctgactt ttcaattgac
10021 agaatctgag tgtgcatata gtaggaccaa agaaaccgcc taaatccact tattcagagt
10081 attcctcttc gatctggaga gcatacaatg taggtgatac ttcatggcat catcccgcac
10141 gactctctcg cgggctcgcg ctccacgggg actcgcttca atttgttccg cggcctattg
10201 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
10261 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
10321 taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat
10381 caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg
10441 tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta
10501 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa
10561 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa
10621 aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa
10681 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac
10741 cctgtctgag ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac
10801 tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc
10861 tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg
10921 cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt
10981 aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt
11041 cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata
11101 cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg
11161 ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt
11221 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
11281 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
11341 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
11401 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagtcgt
11461 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
11521 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
11581 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggtttgtgc acacagccca
11641 gcttggagcg aacgacctac accgaactga gatactaacc attatttttt tcctcaacat
11701 aacgagaaca cacaggggcg ctatcgcaca gaatcaaatt cgatgactgg aaattttttg
11761 ttaatttcag aggtcgcctg acgcatatac ctttttcaac tgaaaaattg ggagaaaaag
11821 gaaaggtgag agcgccggaa ccggcttttc atatagaata gagaagcgtt catgactaaa
11881 tgcttgcatc acaatacttg aagttgacaa tattatttaa ggacctattg tttttccaat
11941 aggtggttag caatcgtctt actttctaac ttttcttacc ttttacattt cagcaatata
12001 tatatatatt tcaaggatat accattctaa tgtctgcccc taagaagatc gtcgttttgc
12061 caggtgacca cgttggtcaa gaaatcacag ccgaagccat taaggttctt aaagctattt
12121 ctgatgttcg ttccaatgtc aagttcgatt tcgaaaatca tttaattggt ggtgctgcta
12181 tcgaagctac aggtgtccca cttccagatg aggcgctgga agcctccaag aaggctgatg
12241 ccgttttgtt aggtgctgtg ggtggtccta aatggggtac aggtagtgtt agacctgaac
12301 aaggtttact aaaaatccgt aaagaacttc aattgtacgc caacttaaga ccatgtaact
12361 ttgcatccga ctctctttta gacttatctc caatcaagcc acaatttgct aaaggtactg
12421 acttcgttgt tgtcagagaa ttagtgggag gtatttactt tggtaagaga aaggaagatg
12481 atggtgatgg tgtcgcttgg gatagtgaac aatacaccgt tccagaagtg caaagaatca
12541 caagaatggc cgctttcatg gccctacaac atgagccacc attgcctatt tggtccttgg
12601 ataaagctaa tgttttggcc tcttcaagat tatggagaaa aactgtggag gaaaccatca
12661 agaacgaatt tcctacattg aaggttcaac atcaattgat tgattctgcc gccatgatcc
12721 tagttaagaa cccaacccac ctaaatggta ttataatcac cagcaacatg tttggtgata
12781 tcatctccga tgaagcctcc gttatcccag gttccttggg tttgttgcca tctgcgtcct
12841 tggcctcttt gccagacaag aacaccgcat ttggtttgta cgaaccatgc cacggttctg
12901 ctccagattt gccaaagaat aaggtcaacc ctatcgccac tatcttgtct gctgcaatga
12961 tgttgaaatt gtcattgaac ttgcctgaag aaggtaaggc cattgaagat gcagttaaaa
13021 aggttttgga tgcaggtatc agaactggtg atttaggtgg ttccaacagt accaccgaag
13081 tcggtgatgc tgtcgccgaa gaagttaaga aaatcctcac ccctgcatct tggaatagct
13141 tgcagtaggt ctaacagtgc tagagctgta ctattgtaag ttgaaacctt ggatacaacc
13201 ttggctcgaa gagatgccaa aacgatcctt tctgctactg atgaatcacc ggcatagagt
13261 attatatgtg cataaatatt gagcagagct gtggcaagtg gccttggctg gaatccgact
13321 aaaaaaataa cacgcaaaaa aaaggaagac gggtgagggg ctgtttgagc cttgattcaa
13381 agtcaaatca tcgcatttgg ctaccataat acctatactt cccagtctat ccagtctgtg
13441 gtttatgatt gcttacaagt gctatccgta tcaggctagg aaaagtggaa gggaaatcgg
13501 ttgaaccaag gccaaaatca ttgtaccatc ttgattgaaa actatataca aagatatcaa
13561 gcatggacat cataaacatg aaacatctga cgattgtgtt tgtagttcca gcccgtggcc
13621 gttgaaacgc ttattctggc atcttgcggc cgctggatga ctccctttcg atgataccct
13681 ttagcagaag tgttcttaaa ttgcgggaag aggccgttca ggagtctggc tcgtttgatc
13741 acatgggatt aaaatctggt gttgaggctg ggatatttta tgttttgtct cttgcgcttc
13801 gatatcaaca gattgacaat aatgaataac tatctacgga ctatgaatgc agctcgatct
13861 actccgtatt tagtcgggca atctcccgta gaagatgcta gactgcgctg aaagactagg
13921 cacagttaat tgccgcggat cacgtcaggc ctgacaaatg acaggtggac cttcatgtaa
13981 tcgcacgatg agtcagcgcc tcttctcccc cgccgacttg cagctgcgga tgtcccgagg
14041 agccgaacat tgaatctatc ccaagagtga ctgaaatatg attgcaatcg tcgtcttttt
14101 cttgcagaga atgtggtgag gtgatcaatc gtgtttttct tcatatatta tattctgtaa
14161 tgatcatact ttgttgagat acccgtctct aaaattgtac tttcgcctcc ctcctccctc
14221 tcaagtccgt catcgcaatg tcgaatttac cgtcccatgc ttctccgtct tttggggcgc
14281 aaacgcctac cacgccgcag cccgtcgacg agctttctct caagtcgaac atcacatcag
14341 cccctgcgtt acactccaga ttagacgaac tccgagatga gacttccagc cagatcagca
14401 gcatagacac tgaccgcggg gacatgaccc ctgcggttcc cgcatcatta ctctccccat
14461 ccttcactcc tccagcaacc cccgggggct cgatcaatac cgcggagctg cttcaacaga
14521 cccaattagc tggcgcacgg caagggtcaa cacatactaa gcccccgagg ttgctttctc
14581 gtcttcccaa tgtcgaatgt atcgtccgcg ctcggatacc cacgacaact ggcgcggaga
14641 tgtttcttca cctgtaccac aatgacttag acaacaaaga gcatttggcg attgtgtttg
14701 gaaatacaat tcgaagcaga agcttggaca agatccggcc tggagagacg gagatggatc
14761 gcatgatccg tggcgcgtat gtcgggaaac tccgaccggg ccgtgtgagc agctggtatg
14821 acgaggagaa agctgaagag gccgccagtc cggaagctgg cggcgctgta tccagtccgc
14881 acacactgcc gcgaagtagc ctcaaggagg cacctcttgt gagaatccat tcggagtgct
14941 acactggtga gacggcttgg tctgcacgct gcgactgcgg cgagcagctc gatgaagcag
15001 ctcgcctcat gtcgctgcct atggaaacgt tggcagaggc tgcgtcaccg ccggatgggg
15061 cggtaccgtc aaatgctgct ggaggtgtaa ttgtgtacct gcgccaagaa ggacgaggca
15121 ttgggctggg cgagaaactg aaagcgtaca atcttcagga tctgggatca gacacggtgg
15181 aagccaatct cctgcttcgc caccctgcgg atgcaagaag ttatggatta gctacggcga
15241 tcctggtgga tcttggtcta gggattgact cgaatccgca cggcatccga ctactcacga
15301 ataacccgga caagattcga gcggttgagg gacccaaccg agaagtggtg gtgaaagagc
15361 gggtgccgat ggtgccattg gcatggagat cgggtggaaa gatgggaatc aagagttccg
15421 aggtcgaagg gtatctgaga acgaaggtat gtcgacaatc tatctcttgc aagctagctt
15481 attttctcat cattcttgaa caatgtctca ctctttcctc gcagatatcg aaaatgggcc
15541 atttgctcca atgaacctat cccctttcgg ttagtaacct tgtgctggaa gtacgccttc
15601 ctgcttttgg acttagccaa gtcgacacgg tgtatgctcg tcacactcat gtaacggttc
15661 tgcagcgcaa acgttctggt cacgttattc gcttttgtcc attttgtctt tttgtgcggt
15721 acataaacta gaggattagc gcactttgta catgatgttc ccgacatgtc acattaggcc
15781 tcgattggtc aaaataatcc ctctctaacc tctggttcgc atactcttaa atgcagctct
15841 agtctctaga cggactagtt cctcctgcag tggatgtctg acgagtgtct gcaaggaccg
15901 ttttccgtgt ccagttagtt ccaatcaccc ggatatcggc gagacgtcgg ccgattgaga
15961 tcactgagtc aatggcgttg agtggcaatt aatatatctg gatgcctaga atcctataaa
16021 ttgggaaaac tagagacccc ggtctatcgt ggtggcagtg cgtcattttt tcctcttgga
16081 gcaaaagtgt agtgccagta cgagtgttgt ggaggaaggc tgcatacatt gtgcctgtca
16141 ttaaacgatg agctcgtccg tattggcccc tgtaatgcca tgttttccgc ccccaatcgt
16201 caaggttttc cctttgttag attcctacca gtcatctagc aagtgaggta agctttgcca
16261 gaaacgccaa ggctttatct atgtagtcga taagcaaagt ggactgatag cttaatatgg
16321 aaggtccctc aggacaagtc gacctgtgca gaagagataa cagcttggca tcacgcatca
16381 gtgcctcctc tcagacagaa taagtcgcca taagttatcg accgaaccta ggtagggtat
16441 atttggtgac gacaatacct cccgacgata cctggattag acaccgtatg ccgcgaatta
16501 acgccttccg accaagtccc ttttggtgat cgacacttta ggatcatctc ctgccagtcg
16561 cccagactga cggtgttatg gtggcgcgct atggctttcc tagccggtca agctccgtgc
16621 actagagagc ggagaaggag ggggagttga tagggaatga tatctaggta gagaagtact
16681 ccaaccagag atatgcggat caatgctccg gcagttgtta gcatacccca acctcgttta
16741 agtatcctct tcttccatcc atctttatca ttatttgtct atgtgcatcc ctagatcacg
16801 tcaattattt cgataaatgg atacgtgcac tcgggaatgc gtgattggct cgtctcgtgt
16861 tctcagttcc tcattccccc ccacgttaca ttgacaacgg agtattagcc tatggtcctg
16921 ttcgataagc ttgctccatc gcctccacta cgtcaagcgg agtatcagac atgagctcta
16981 ccccagatat cgcctgctta tcgctggttg ctttgtcatg caagtacaga tcacatcggc
17041 aattctgata acagatggtg ccattgtccc gtaatcaatt gcccattgaa aaaggaagag
17101 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
17161 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
17221 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
17281 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
17341 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
17401 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
17461 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
17521 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
17581 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
17641 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
17701 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
17761 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
17821 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
17881 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
17941 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
18001 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
18061 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
18121 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
18181 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
18241 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
18301 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
18361 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
18421 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
18481 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
18541 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
18601 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
18661 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
18721 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
18781 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
18841 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
18901 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
18961 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
19021 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
19081 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccactga
19141 acgaagcatc tgtgcttcat tttgtagaac aaaaatgcaa cgcgagagcg ctaatttttc
19201 aaacaaagaa tctgagctgc atttttacag aacagaaatg caacgcgaaa gcgctatttt
19261 accaacgaag aatctgtgct tcatttttgt aaaacaaaaa tgcaacgcga gagcgctaat
19321 ttttcaaaca aagaatctga gctgcatttt tacagaacag aaatgcaacg cgagagcgct
19381 attttaccaa caaagaatct atacttcttt tttgttctac aaaaatgcat cccgagagcg
19441 ctatttttct aacaaagcat cttagattac tttttttctc ctttgtgcgc tctataatgc
19501 agtctcttga taactttttg cactgtaggt ccgttaaggt tagaagaagg ctactttggt
19561 gtctattttc tcttccataa aaaaagcctg actccacttc ccgcgtttac tgattactag
19621 cgaagctgcg ggtgcatttt ttcaagataa aggcatcccc gattatattc tataccgatg
19681 tggattgcgc atactttgtg aacagaaagt gatagcgttg atgattcttc attggtcaga
19741 aaattatgaa cggtttcttc tattttgtct ctatatacta cgtataggaa atgtttacat
19801 tttcgtattg ttttcgattc actctatgaa tagttcttac tacaattttt ttgtctaaag
19861 agtaatacta gagataaaca taaaaaatgt agaggtcgag tttagatgca agttcaagga
19921 gcgaaaggtg gatgggtagg ttatataggg atatagcaca gagatatata gcaaagagat
19981 acttttgagc aatgtttgtg gaagcggtat tcgcaatatt ttagtagctc gttacagtcc
20041 ggtgcgtttt tggttttttg aaagtgcgtc ttcagagcgc ttttggtttt caaaagcgct
20101 ctgaagttcc tatactttct agagaatagg aacttcggaa taggaacttc aaagcgtttc
20161 cgaaaacgag cgcttccgaa aatgcaacgc gagctgcgca catacagctc actgttcacg
20221 tcgcacctat atctgcgtgt tgcctgtata tatatataca tgagaagaac ggcatagtgc
20281 gtgtttatgc ttaaatgcgt acttatatgc gtctatttat gtaggatgaa aggtagtcta
20341 gtacctcctg tgatattatc ccattccatg cggggtatcg tatgcttcct tcagcactac
20401 cctttagctg ttctatatgc tgccactcct caattggatt agtctcatcc ttcaatgcta
20461 tcatttcctt tgatattgga tcatactaag aaaccattat tatcatgaca ttaacctata
20521 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
20581 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
20641 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
20701 cggcatcaga gcagattgta ctgagagtgc accataccac cttttcaatt catcattttt
20761 tttttattct tttttttgat ttcggtttcc ttgaaatttt tttgattcgg taatctccga
20821 acagaaggaa gaacgaagga aggagcacag acttagattg gtatatatac gcatatgtag
20881 tgttgaagaa acatgaaatt gcccagtatt cttaacccaa ctgcacagaa caaaaacctg
20941 caggaaacga agataaatca tgtcgaaagc tacatataag gaacgtgctg ctactcatcc
21001 tagtcctgtt gctgccaagc tatttaatat catgcacgaa aagcaaacaa acttgtgtgc
21061 ttcattggat gttcgtacca ccaaggaatt actggagtta gttgaagcat taggtcccaa
21121 aatttgttta ctaaaaacac atgtggatat cttgactgat ttttccatgg agggcacagt
21181 taagccgcta aaggcattat ccgccaagta caatttttta ctcttcgaag acagaaaatt
21241 tgctgacatt ggtaatacag tcaaattgca gtactctgcg ggtgtataca gaatagcaga
21301 atgggcagac attacgaatg cacacggtgt ggtgggccca ggtattgtta gcggtttgaa
21361 gcaggcggca gaagaagtaa caaaggaacc tagaggcctt ttgatgttag cagaattgtc
21421 atgcaagggc tccctatcta ctggagaata tactaagggt actgttgaca ttgcgaagag
21481 cgacaaagat tttgttatcg gctttattgc tcaaagagac atgggtggaa gagatgaagg
21541 ttacgattgg ttgattatga cacccggtgt gggtttagat gacaagggag acgcattggg
21601 tcaacagtat agaaccgtgg atgatgtggt ctctacagga tctgacatta ttattgttgg
21661 aagaggacta tttgcaaagg gaagggatgc taaggtagag ggtgaacgtt acagaaaagc
21721 aggctgggaa gcatatttga gaagatgcgg ccagcaaaac taaaaaactg tattataagt
21781 aaatgcatgt atactaaact cacaaattag agcttcaatt taattatatc agttattacc
21841 ctatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggaaattgt
21901 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
21961 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
22021 gagtggggta ccgagctcga attcactggc cgtcgtttta caacgtcgtg actgggaaaa
22081 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
22141 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
22201 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca
22261 aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
22321 cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct
22381 tcctttctcg ccacgttcgc cagcttattt tttgtatact gttttgtgat agcacgaagt
22441 ttttccacgg tatcttgtta aaaatatata tttgtggcgg gcttacctac atcaaattaa
22501 taagagacta attataaact aaacacacaa gcaagctact ttagggtaaa agtttataaa
22561 tgcttttgac gtataaacgt tgcttgtatt tattattaca attaaaggtg gatagaaaac
22621 ctagagacta gttagaaact aatctcaggt ttgcgttaaa ctaaatcaga gcccgagagg
22681 ttaacagaac ctagaagggg actagatatc cgggtaggga aacaaaaaaa aaaaacaaga
22741 cagccacata ttagggagac tagttagaag ctagttccag gactaggaaa ataaaagaca
22801 atgataccac agtctagttg acaactagat agattctaga ttgaggccaa agtctctgag
22861 atccaggtta gttgcaacta atactagtta gtatctagtc tcctataact ctgaagctag
22921 aataacttac tactattatc ctcaccactg ttcagctgcg caaacggagt gattgcaagg
22981 tgttcagaga ctagttattg actagtcagt gactagcaat aactaacaag gtattaacct
23041 accatgtctg ccatcaccct gcacttcctc gggctcagca gccttttcct cctcattttc
23101 atgctcattt tccttgttta agactgtgac tagtcaaaga ctagtccaga accacaaagg
23161 agaaatgtct taccactttc ttcattgctt gtctcttttg cattatccat gtctgcaact
23221 agttagagtc tagttagtga ctagtccgac gaggacttgc ttgtctccgg attgttggag
23281 gaactctcca gggcctcaag atccacaaca gagccttcta gaagactggt caataactag
23341 ttggtctttg tctgagtctg acttacgagg ttgcatactc gctccctttg cctcgtcaat
23401 cgatgagaaa aagcgccaaa actcgcaata tggctttgaa ccacacggtg ctgagactag
23461 ttagaatcta gtcccaaact agcttggata gcttaccttt gccctttgcg ttgcgacagg
23521 tcttgcaggg tatggttcct ttctcaccag ctgatttagc tgccttgcta ccctcacggc
23581 ggatctgcat aaagagtggc tagaggttat aaattagcac tgatcctagg tacggggctg
23641 aatgtaactt gcctttcctt tctcatcgcg cggcaagaca ggcttgctca aattcctacc
23701 agtcacaggg gtatgcacgg cgtacggacc acttgaacta gtcacagatt agttagcaac
23761 tagtctgcat tgaatggctg tacttacggg ccctcgccat tgtcctgatc atttccagct
23821 tcaccctcgt tgctgcaaag tagttagtga ctagtcaagg actagttgaa atgggagaag
23881 aaactcacga attctcgaca cccttagtat tgtggtcctt ggacttggtg ctgctatata
23941 ttagctaata cactagttag actcacagaa acttacgcag ctcgcttgcg cttcttggta
24001 ggagtcgggg ttgggagaac agtgccttca aacaagcctt cataccatgc tacttgacta
24061 gtcagggact agtcaccaag taatctagat aggacttgcc tttggcctcc atcagttcct
24121 tcatagtggg aggtccattg tgcaatgtaa actccatgcc gtgggagttc ttgtccttca
24181 agtgcttgac caatatgttt ctgttggcag agggaacctg tcaactagtt aataactagt
24241 cagaaactag tatagcagta gactcactgt acgcttgagg catcccttca ctcggcagta
24301 gacttcatat ggatggatat caggcacgcc attgtcgtcc tgtggactag tcagtaacta
24361 ggcttaaagc tagtcgggtc ggcttactat cttgaaatcc ggcagcgtaa gctccccgtc
24421 cttaactgcc tcgagatagt gacagtactc tggggacttt cggagatcgt tatcgcgaat
24481 gctcggcata ctaatcgttg actagtcttg gactagtccc gagcaaaaag gattggagga
24541 ggaggaggaa ggtgagagtg agacaaagag cgaaataaga gcttcaaagg ctatctctaa
24601 gcagtatgaa ggttaagtat ctagttcttg actagattta aaagagattt cgactagtta
24661 tgtacctgga gtttggatat aggaatgtgt tgtggtaacg aaatgtaagg gggaggaaag
24721 aaaaagtcgg tcaagaggta actctaagtc ggccattcct ttttgggagg cgctaaccat
24781 aaacggcatg gtcgacttag agttagctca gggaatttag ggagttatct gcgaccactg
24841 gactctcagg ctttccattg gggtactaac atagccatca aatgccggcc cgtgtttaat
24901 ctcctcgaga tggctgggct cacatgcgtt ctactcaaaa actcttatac ctaggccctc
24961 tcccaatcgt gtatgtttgg accttcctct ctcacacttt ggataacatc aagcatac
//
