pFB-Neo

价格:3000元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Agilent
载体名称: pFB-Neo
质粒类型: 逆病毒载体;双顺反子载体
高拷贝/低拷贝: 低拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: --
载体大小: 6572 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄青霉素
筛选标记: 新霉素(Neomycin)
克隆菌株: DH5α 等通用菌株
宿主细胞(系): 常规细胞系(293、CV-1、CHO等)
备注: --
产品目录号: #217561
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 逆转录病毒

载体质粒图谱和多克隆位点信息

pFB-Neo载体图谱



pFB-Neo 多克隆位点

pFB-Neo 载体特征

载体简介

pFB-Neo载体描述

The Agilent pFB and pFB-Neo plasmid vectors for retroviral gene delivery and expression are derived from the Moloney murine leukemia virus (MMLV) and can be used to produce high titer viral stocks with MMLVbased packaging cell lines.1,2 Both vectors contain the bacterial origin of replication and ampicillin-resistance gene from pBR322, an extended MMLV packaging signal (ψ+), and a multiple cloning site (MCS) located between the MMLV 5′ and 3′ long terminal repeat sequences (LTRs), see Figures 1 and 2.

The pFB vector is a minimal MMLV-based vector that can accommodate larger inserts than pFB-Neo. The pFB vector does not contain any exogenous sequence other than the sequence of the MCS. The pFB-Neo vector differs from the pFB vector only by the presence of a cassette consisting of the internal ribosome entry site (IRES) from the encephalomyocarditis virus (EMCV) and the neomycin-resistance gene(neor). The open reading frame (ORF) for the neor gene is positioned downstream of the MCS and follows the IRES. A dicistronic message encoding the gene of interest and the neor gene is expressed from the viral promoter within the 5′ LTR.



pFB-Neo载体限制性酶切位点

pFB-Neo, 6572 bp			       version 037004

Enzymes with 1-10 cleavage sites:
           
  
            #sites   --  Bp position of recognition site --

  AarI           1     2348
  AatII          2      978,   6498
  Acc65I         4      647,   1847,   2460,   4098
  AccI           3     1968,   2058,   4458
  AclI           2     5805,   6178
  AcuI           6     2443,   2613,   3017,   3449,   5214,   6262
  AflII          3      202,   1265,   3653
  AflIII         4      164,   2334,   2509,   4687
  AgeI           1     1841
  AhdI           6      687,    733,   1274,   4138,   4184,   5575
  AleI           1     2658
  Alw44I         5     1054,   2496,   4503,   5001,   6247
  AlwNI          5      323,    398,   3774,   3849,   5098
  ApaI           2     1238,   2132
  ApoI           3       87,   1128,   2064
  AseI           1     5751
  AvaI           9      577,    610,    643,   1241,   2019,   2075
                       4028,   4061,   4094
  BamHI          1     2070
  BanII          9      580,    593,    764,   1238,   2132,   3253
                       4031,   4044,   4215
  BbeI           4      615,   1656,   2891,   4066
  BbsI           4     2152,   2252,   2618,   6565
  BbvCI          2      453,   3904
  BceAI          7      999,   1399,   1972,   2112,   2866,   3302
                       5173
  BciVI          6      656,   1987,   3100,   4107,   4896,   6423
  BglI           2     2315,   5694
  BglII          1     1678
  BlnI           1     2170
  BmgBI          1     2559
  BmrI           4     2369,   2831,   4433,   5625
  BmtI           5        6,     16,     26,    197,   3648
  BpmI           3     1799,   3601,   5665
  Bpu10I         7      337,    412,    453,   1548,   3788,   3863
                       3904
  BpuEI          6     2421,   3283,   4793,   5055,   5332,   6200
  BsaAI          4     2001,   2333,   3194,   4439
  BsaHI          7      615,    978,   1656,   2891,   4066,   6116
                       6498
  BsaI           9      694,    715,    782,   1414,   1802,   4145
                       4166,   4233,   5647
  BsaWI          7      138,   1841,   2090,   2923,   4893,   5040
                       5871
  BseRI          7      718,   1068,   1561,   1600,   2418,   2670
                       4169
  BseYI          2     3396,   4991
  BsgI           1     2719
  BsiEI         10      961,   1034,   1839,   2081,   2583,   2798
                       4600,   5024,   5947,   6096
  BsiHKAI       10      580,   1054,   2496,   3001,   3191,   4031
                       4503,   5001,   6162,   6247
  BsmBI          6      976,   1093,   1337,   1396,   1582,   4336
  BsmI           2     2165,   2198
  BspHI          3     5407,   6415,   6520
  BspMI          3     2349,   2788,   3151
  BsrBI          6     2078,   2644,   3503,   3557,   4618,   6419
  BsrDI          4     2123,   3118,   5634,   5816
  BsrGI          1     1541
  BssHII         3      563,   3289,   4014
  BssSI          4     3484,   4860,   6244,   6551
  Bst1107I       1     4458
  BstAPI         5      322,    397,   3773,   3848,   4505
  BstEII         1     1346
  Bsu36I         1     1276
  BtgI           6      151,    931,   2552,   2702,   2747,   3324
  BtgZI          4     1976,   3178,   3329,   3522
  BtsI           2     5973,   6001
  Cfr10I         4     1841,   3211,   3392,   5660
  ClaI           1     2585
  Csp6I          9      648,    895,   1542,   1848,   2461,   3197
                       4099,   4494,   6059
  DraI           3     5444,   5463,   6155
  DraIII         2     1874,   2377
  DrdI           3     2913,   4376,   4789
  EagI           3      961,   2081,   2798
  EarI           6     1334,   1564,   3241,   3451,   4565,   6369
  EciI           8      924,   1556,   1575,   1885,   3405,   4759
                       4905,   5733
  Eco57MI        9     1799,   2443,   2613,   3017,   3449,   3601
                       5214,   5665,   6262
  EcoICRI        2      580,   4031
  EcoNI          1     1647
  EcoO109I       8      499,   1477,   1925,   2131,   2487,   2540
                       3950,   6555
  EcoRI          1     2064
  EcoRV          4      308,    383,   3759,   3834
  FspI           2     2992,   5800
  HaeII          6      615,   1656,   2891,   4066,   4561,   4931
  HgaI           6      889,   1166,   4393,   4789,   5367,   6117
  HincII         2     2058,   6119
  HindIII        1     2243
  KasI           4      615,   1656,   2891,   4066
  KpnI           4      647,   1847,   2460,   4098
  MlyI          10      637,   1020,   1045,   1864,   2405,   3563
                       4088,   4587,   5058,   5575
  MmeI           6      684,   1383,   2387,   4135,   4877,   5061
  MscI           4      827,   1368,   1668,   2972
  MslI           6      159,   2658,   3325,   5828,   5987,   6346
  MunI           2       11,     21
  NaeI           1     3392
  NarI           4      615,   1656,   2891,   4066
  NcoI           3     2702,   2747,   3324
  NdeI           3     1664,   1672,   4509
  NgoMIV         1     3392
  NheI           5        6,     16,     26,    197,   3648
  NotI           1     2080
  NspI           6      164,   2500,   2509,   3293,   4322,   4687
  PciI           3      164,   2509,   4687
  PfoI           3      771,   4222,   4331
  PleI          10      637,   1020,   1045,   1864,   2405,   3563
                       4088,   4587,   5058,   5575
  PmlI           2     2001,   2333
  PpuMI          4      499,   1477,   1925,   3950
  PshAI          1     1015
  PspOMI         2     1238,   2132
  PstI           4     1178,   1360,   2941,   5821
  PvuI           3     1034,   2583,   5947
  PvuII          5      286,    361,   2996,   3737,   3812
  RsaI           9      648,    895,   1542,   1848,   2461,   3197
                       4099,   4494,   6059
  RsrII          1     3407
  SacI           2      580,   4031
  SacII          1      151
  SalI           1     2058
  SapI           3     3241,   3451,   4564
  ScaI           1     6058
  SexAI          1     1474
  SfcI           9      182,   1178,   1360,   2267,   2941,   3633
                       4952,   5143,   5821
  SfoI           4      615,   1656,   2891,   4066
  SmaI           4      643,   1241,   2019,   4094
  SmlI          10      202,   1265,   2075,   2421,   3283,   3653
                       4793,   5055,   5332,   6200
  SpeI           1      897
  SphI           1     3293
  SrfI           1     1240
  SspI           1     6382
  StyI           9      496,    706,   1504,   2170,   2702,   2747
                       3324,   3947,   4157
  TatI           3     1541,   4493,   6058
  TfiI           3     3377,   3511,   4662
  Tsp45I         8     1282,   1491,   3013,   3319,   4342,   4437
                       5837,   6048
  TspDTI         9       34,    755,   2936,   3379,   3619,   4206
                       5456,   5558,   5861
  TspGWI         3     1121,   6030,   6347
  Tth111I        5      633,   1473,   3007,   4084,   4431
  Van91I         1     2466
  XbaI           3      464,   2037,   3915
  XhoI           1     2075
  XmaI           4      643,   1241,   2019,   4094
  XmnI           2     2227,   6175
  ZraI           2      978,   6498



Enzymes that do NOT cut molecule:

AccIII      AscI        AsiSI       BclI        BfrBI       
BlpI        BsaBI       BsiWI       BstBI       BstXI       
Eco47III    FseI        FspAI       HpaI        MluI        
NruI        NsiI        PacI        PmeI        PsiI        
SanDI       SbfI        SfiI        SgrAI       SnaBI       
StuI        SwaI        XcmI        

载体序列

pFB-Neo, 6572 bp                               version 037003

NOTE: The following sequence has been verified for accuracy
at the junctions. The remainder of the sequence has been 
obtained from existing data. 



    1  GAATTGCTAG CAATTGCTAG CAATTGCTAG CAATTCATAC CAGATCACCG
   51  AAAACTGTCC TCCAAATGTG TCCCCCTCAC ACTCCCAAAT TCGCGGGCTT
  101  CTGCCTCTTA GACCACTCTA CCCTATTCCC CACACTCACC GGAGCCAAAG
  151  CCGCGGGACA TATACATGTG AAAGACCCCA CCTGTAGGTT TGGCAAGCTA
  201  GCTTAAGTAA CGCCATTTTG CAAGGCATGG AAAAATACAT AACTGAGAAT
  251  AGAAAAGTTC AGATCAAGGT CAGGAACAGA TGGAACAGCT GAATATGGGC
  301  CAAAGCGGAT ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC AGGGCCAAGA
  351  ACAGATGGAA CAGCTGAATA TGGGCCAAAC AGGATATCTG TGGTAAGCAG
  401  TTCCTGCCCC GGCTCAGGGC CAAGAACAGA TGGTCCCCAG ATGCGGTCCA
  451  GCCCTCAGCA GTTTCTAGAG AACCATCAGA TGTTTCCAGG GTGCCCCAAG
  501  GACCTGAAAT GACCCTGTGC CTTATTTGAA CTAACCAATC AGTTCGCTTC
  551  TCGCTTCTGT TCGCGCGCTT CTGCTCCCCG AGCTCAATAA AAGAGCCCAC
  601  AACCCCTCAC TCGGGGCGCC AGTCCTCCGA TTGACTGAGT CGCCCGGGTA
  651  CCCGTGTATC CAATAAACCC TCTTGCAGTT GCATCCGACT TGTGGTCTCG
  701  CTGTTCCTTG GGAGGGTCTC CTCTGAGTGA TTGACTACCC GTCAGCGGGG
  751  GTCTTTCATT TGGGGGCTCG TCCGGGATCG GGAGACCCCT GCCCAGGGAC
  801  CACCGACCCA CCACCGGGAG GTAAGCTGGC CAGCAACTTA TCTGTGTCTG
  851  TCCGATTGTC TAGTGTCTAT GACTGATTTT ATGCGCCTGC GTCGGTACTA
  901  GTTAGCTAAC TAGCTCTGTA TCTGGCGGAC CCGTGGTGGA ACTGACGAGT
  951  TCGGAACACC CGGCCGCAAC CCTGGGAGAC GTCCCAGGGA CTTCGGGGGC
 1001  CGTTTTTGTG GCCCGACCTG AGTCCAAAAA TCCCGATCGT TTTGGACTCT
 1051  TTGGTGCACC CCCCTTAGAG GAGGGATATG TGGTTCTGGT AGGAGACGAG
 1101  AACCTAAAAC AGTTCCCGCC TCCGTCTGAA TTTTTGCTTT CGGTTTGGGA
 1151  CCGAAGCCGC GCCGCGCGTC TTGTCTGCTG CAGCATCGTT CTGTGTTGTC
 1201  TCTGTCTGAC TGTGTTTCTG TATTTGTCTG AAAATATGGG CCCGGGCCAG
 1251  ACTGTTACCA CTCCCTTAAG TTTGACCTTA GGTCACTGGA AAGATGTCGA
 1301  GCAGATCGCT CACAACCAGT CGGTAGATGT CAAGAAGAGA CGTTGGGTTA
 1351  CCTTCTGCTC TGCAGAATGG CCAACCTTTA ACGTCGGATG GCCGCGAGAC
 1401  GGCACCTTTA ACCGAGACCT CATCACCCAG GTTAAGATCA AGGTCTTTTC
 1451  ACCTGGCCCG CATGGACACC CAGACCAGGT CCCCTACATC GTGACCTGGG
 1501  AAGCCTTGGC TTTTGACCCC CCTCCCTGGG TCAAGCCCTT TGTACACCCT
 1551  AAGCCTCCGC CTCCTCTTCC TCCATCCGCC CCGTCTCTCC CCCTTGAACC
 1601  TCCTCGTTCG ACCCCGCCTC GATCCTCCCT TTATCCAGCC CTCACTCCTT
 1651  CTCTAGGCGC CCCCATATGG CCATATGAGA TCTTATATGG GGCACCCCCG
 1701  CCCCTTGTAA ACTTCCCTGA CCCTGACATG ACAAGAGTTA CTAACAGCCC
 1751  CTCTCTCCAA GCTCACTTAC AGGCTCTCTA CTTAGTCCAG CACGAAGTCT
 1801  GGAGACCTCT GGCGGCAGCC TACCAAGAAC AACTGGACCG ACCGGTGGTA
 1851  CCTCACCCTT ACCGAGTCGG CGACACAGTG TGGGTCCGCC GACACCAGAC
 1901  TAAGAACCTA GAACCTCGCT GGAAAGGACC TTACACAGTC CTGCTGACCA
 1951  CCCCCACCGC CCTCAAAGTA GACGGCATCG CAGCTTGGAT ACACGCCGCC
 2001  CACGTGAAGG CTGCCGACCC CGGGGGTGGA CCATCCTCTA GACTGCCGGA
 2051  TCGAATTGTC GACGAATTCG GATCCTCGAG CGGCCGCGAT CCGGTTATTT
 2101  TCCACCATAT TGCCGTCTTT TGGCAATGTG AGGGCCCGGA AACCTGGCCC
 2151  TGTCTTCTTG ACGAGCATTC CTAGGGGTCT TTCCCCTCTC GCCAAAGGAA
 2201  TGCAAGGTCT GTTGAATGTC GTGAAGGAAG CAGTTCCTCT GGAAGCTTCT
 2251  TGAAGACAAA CAACGTCTGT AGCGACCCTT TGCAGGCAGC GGAACCCCCC
 2301  ACCTGGCGAC AGGTGCCTCT GCGGCCAAAA GCCACGTGTA TAAGATACAC
 2351  CTGCAAAGGC GGCACAACCC CAGTGCCACG TTGTGAGTTG GATAGTTGTG
 2401  GAAAGAGTCA AATGGCTCTC CTCAAGCGTA TTCAACAAGG GGCTGAAGGA
 2451  TGCCCAGAAG GTACCCCATT GTATGGGATC TGATCTGGGG CCTCGGTGCA
 2501  CATGCTTTAC ATGTGTTTAG TCGAGGTTAA AAAACGTCTA GGCCCCCCGA
 2551  ACCACGGGGA CGTGGTTTTC CTTTGAAAAA CACGATCGAT AATATGGAAC
 2601  AAAAACTTAT TTCTGAAGAA GACTTGGACA CCAAACTTTC CTGCCGCTCG
 2651  ATTTCTCCAC CCAGGTGAAC TCCTCCCTCA CCTCCCCGAC GGGGCGAGGC
 2701  TCCATGGCTG CCCCCTCGCT GCACCCGTCC CTGGTGCCAC GCGGTTCCAT
 2751  GGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG
 2801  CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC
 2851  GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT
 2901  TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG
 2951  AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT
 3001  GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA
 3051  AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG
 3101  TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT
 3151  ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC
 3201  TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC
 3251  AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC
 3301  GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT
 3351  CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG
 3401  GTGTGGCGGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT
 3451  GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT
 3501  CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT
 3551  TCTTCTGAGC GGGACTCTGG GGTTCGATAA AATAAAAGAT TTTATTTAGT
 3601  CTCCAGAAAA AGGGGGGAAT GAAAGACCCC ACCTGTAGGT TTGGCAAGCT
 3651  AGCTTAAGTA ACGCCATTTT GCAAGGCATG GAAAAATACA TAACTGAGAA
 3701  TAGAGAAGTT CAGATCAAGG TCAGGAACAG ATGGAACAGC TGAATATGGG
 3751  CCAAACAGGA TATCTGTGGT AAGCAGTTCC TGCCCCGGCT CAGGGCCAAG
 3801  AACAGATGGA ACAGCTGAAT ATGGGCCAAA CAGGATATCT GTGGTAAGCA
 3851  GTTCCTGCCC CGGCTCAGGG CCAAGAACAG ATGGTCCCCA GATGCGGTCC
 3901  AGCCCTCAGC AGTTTCTAGA GAACCATCAG ATGTTTCCAG GGTGCCCCAA
 3951  GGACCTGAAA TGACCCTGTG CCTTATTTGA ACTAACCAAT CAGTTCGCTT
 4001  CTCGCTTCTG TTCGCGCGCT TCTGCTCCCC GAGCTCAATA AAAGAGCCCA
 4051  CAACCCCTCA CTCGGGGCGC CAGTCCTCCG ATTGACTGAG TCGCCCGGGT
 4101  ACCCGTGTAT CCAATAAACC CTCTTGCAGT TGCATCCGAC TTGTGGTCTC
 4151  GCTGTTCCTT GGGAGGGTCT CCTCTGAGTG ATTGACTACC CGTCAGCGGG
 4201  GGTCTTTCAT TTGGGGGCTC GTCCGGGATC GGGAGACCCC TGCCCAGGGA
 4251  CCACCGACCC ACCACCGGGA GGTAAGCTGG CTGCCTCGCG CGTTTCGGTG
 4301  ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT
 4351  TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC
 4401  GGGTGTTGGC GGGTGTCGGG GCGCAGCCAT GACCCAGTCA CGTAGCGATA
 4451  GCGGAGTGTA TACTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA
 4501  GAGTGCACCA TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA
 4551  TACCGCATCA GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC
 4601  GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC
 4651  GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA
 4701  GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT
 4751  CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC
 4801  AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT
 4851  GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA
 4901  CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC
 4951  GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT
 5001  GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA
 5051  TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG
 5101  CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG
 5151  TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG
 5201  TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT
 5251  CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC
 5301  AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT
 5351  CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA
 5401  TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT
 5451  TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC
 5501  TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC
 5551  TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG
 5601  ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA
 5651  CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA
 5701  GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT
 5751  ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT
 5801  GCGCAACGTT GTTGCCATTG CTGCAGGCAT CGTGGTGTCA CGCTCGTCGT
 5851  TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA
 5901  TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT
 5951  CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG
 6001  CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG
 6051  ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC
 6101  GAGTTGCTCT TGCCCGGCGT CAACACGGGA TAATACCGCG CCACATAGCA
 6151  GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC
 6201  TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC
 6251  ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG
 6301  CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG
 6351  AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA
 6401  TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA
 6451  ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC
 6501  GTCTAAGAAA CCATTATTAT CATGACATTA ACCTATAAAA ATAGGCGTAT
 6551  CACGAGGCCC TTTCGTCTTC AA

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载体应用举例

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