LOCUS pAAVLINKv1-pCALM1-Cre-3'-CUX2-FLAG 6705 bp DNA circular SYN 14-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6705)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 12, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6705
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3418
/codon_start=1
/label=CUX2
/translation="GRRTNPPGRASSRLRARRPRRARGCPTTRPTCRAP*SPPCRR*PP
SSTSCTCTVR*TRWSSPARSRRSWPRTASARGSSGRRCWACHRAA*ATCCPGRSHGAS*
RRRGGSPSSACSCGSLTSSARQWASSLVPPRPVPQNQGPHHPHPPAPQSLRRAPRSR*A
CPWRAARRTSSQRAAPAPR*AGRCTQAARPQGASRRSWPCPPSWTRTPSPRG*RRSSQT
TI*GSGCLGKASWV*HRAPCLTCCPGPNPGTS*A*RGGSLLSACSCGSMTPITWRS*GI
*RSWRRKPT*NVAMASSAPAQTVSPRPPAQSAPAPACSPRT*ASCRSRSPGWCWHPRRR
RHCGRPISWNPTPRSRPSSSSPSSSTSRPTPSSTGSTTTGPGCAGRCWWRGPRMSQTLI
QAGVLESYRQATPTQTPPRRALTLRLRTRSQP*RNWSFRRALRRTAHP*PPRTRPK*GS
SRNRWRRMLRKRQAASPRTQGSWTKAKVPPKRSIPTLRVMMDSQKWLPGPSFQVDPPQT
VPHFIPNRRVRPGSDFTRTL*VLSQPQSPHAAAWRCH*TRPRPPPHQAS*CLCHLSPPP
QLPSPHPHLAPPLPKCRVPAPLLTWLEPCTPVPR*TPTCSGGMRRWPI*TTSFTE*SGL
PIGRRPWSGS"
misc_feature 2130..2135
/label=SDS
misc_feature 2452..2457
/label=SDS
misc_feature 2499..2504
/label=SDS
misc_feature 2793..2798
/label=SDS
misc_feature 2871..2876
/label=SDS
misc_feature 3004..3009
/label=SDS
misc_feature 3162..3167
/label=SDS
misc_feature 3201..3206
/label=SDS
misc_feature 3204..3209
/label=SDS
misc_feature 3396..3401
/label=SDS
CDS 3425..3490
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3494..3925
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3638..3643
/label=SDS
repeat_region 3968..4108
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3968..4097
/label=AAV2 ITR
rep_origin 4183..4638
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4920..5024
/gene="bla"
/label=AmpR promoter
CDS 5025..5885
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6056..6644
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca ggggcggagg acgaaccccc
1501 caggacgggc gagctcaagg ctgagggcgc gacggccgag gcgggcgcgc ggctgcccta
1561 ctacccggcc tacgtgccgc gcaccctgaa gcccaccgtg ccgccgctga cccccgagca
1621 gtacgagctg tacatgtacc gtgaggtaga cacgctggag ctcacccgcc aggtcaagga
1681 gaagctggcc aagaacggca tctgccagag gatcttcggg gagaaggtgc tgggcctgtc
1741 acagggcagc gtgagcgaca tgctgtcccg gccgaagcca tggagcaagc tgacgcagaa
1801 ggggcgggag cccttcatcc gcatgcagct gtggctctct gaccagctcg gccaggcagt
1861 gggccagcag cctggtgcct cccaggccag tcccacagaa ccaaggtcct caccatcccc
1921 accccccagc cccacagagc ctgagaagag ctcccaggag ccgttgagcc tgtccctgga
1981 gagcagcaag gagaaccagc agccagaggg ccgctccagc tcctcgttga gcgggaagat
2041 gtactcaggc agccaggccc cagggggcat ccaggagatc gtggccatgt cccccgagct
2101 ggacacgtac tccatcacca agagggtgaa ggaggtcctc acagacaaca atctagggca
2161 gcggctgttt ggggaaagca tcctgggtct gacacagggc tccgtgtctg acctgctgtc
2221 ccggcccaaa ccctggcaca agctgagcct gaaggggcgg gagccttttg tccgcatgca
2281 gctgtggctc aatgaccccc ataacgtgga gaagctgagg gatatgaaga agctggagaa
2341 gaaagcctac ctgaaacgtc gctatggcct catcagcacc ggctcagaca gtgagtcccc
2401 ggccacccgc tcagagtgcc ccagcccctg cctgcagccc caggacctga gcctcctgca
2461 gatcaagaag ccccgggtgg tgctggcacc cgaggagaag gaggcactgc ggaaggccta
2521 tcagctggaa ccctacccct cgcagcagac catcgagctc ctctccttcc agctcaacct
2581 caagaccaac accgtcatca actggttcca caactacagg tcccggatgc gccgggagat
2641 gttggtggag gggacccagg atgagccaga ccttgatcca agcgggggtc ctggaatcct
2701 accgccaggc cactcccacc cagaccccac cccgcagagc cctgactctg agactgagga
2761 ccagaagcca accgtgaagg aactggagct tcaggagggc cctgaggaga acagcacacc
2821 cctgaccacc caggacaagg cccaagtgag gatcaagcag gaacagatgg aggaggatgc
2881 tgaggaagag gcaggcagcc agccccagga ctcaggggag ctggacaaag gccaaggtcc
2941 ccccaaagag gagcatcccg accctccggg taatgatgga ctcccaaaag tggctcccgg
3001 gcccctcctt ccaggtggat ccaccccaga ctgtccctca cttcatcccc aacaggagag
3061 tgaggccggg gagcgacttc acccggaccc tttaagtttt aagtcagcct cagagtcctc
3121 acgctgcagc ctggaggtgt cactgaactc gccctcggcc gcctcctcac caggcctcat
3181 gatgtctgtg tcacctgtcc cctcctcctc agctcccatc tccccatccc cacctggcgc
3241 cccccctgcc aaagtgccga gtgccagccc cactgctgac atggctggag ccttgcaccc
3301 cagtgccaag gtgaacccca acttgcagcg gcggcatgag aagatggcca atctgaacaa
3361 catcatttac cgagtagagc gggctgccaa tcgggaggag gccctggagt gggagttctg
3421 tacagactac aaagaccatg acggtgatta taaagatcat gacatcgact acaaggatga
3481 cgatgacaag taactcgaga taatcaacct ctggattaca aaatttgtga aagattgact
3541 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
3601 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggtta
3661 gttcttgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
3721 ctgttgggca ctgacaattc cgtggtgttt atttgtgaaa tttgtgatgc tattgcttta
3781 tttgtaacca tctagcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
3841 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
3901 gggggagatg tgggaggttt tttaaagatc tggagaccgg tgtggcacgt gcggaccgag
3961 cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc
4021 actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg
4081 agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat
4141 ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg
4201 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct
4261 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
4321 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
4381 accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
4441 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
4501 gaacaacact caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt
4561 cggtctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
4621 tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
4681 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
4741 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
4801 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
4861 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
4921 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
4981 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
5041 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
5101 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
5161 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
5221 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
5281 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
5341 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
5401 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
5461 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
5521 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
5581 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
5641 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
5701 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
5761 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
5821 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
5881 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
5941 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
6001 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
6061 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
6121 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
6181 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
6241 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
6301 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
6361 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
6421 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
6481 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
6541 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
6601 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
6661 cctttttacg gttcctggcc ttttgctggc cttttgctca catgt
//
