| 质粒名称: | AbVec2.0-mIgkc |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 127157 |
| 存储实验室: | Hedda Wardemann |
|
载体骨架基本信息 |
|
| 载体名称: | pUC19 |
| 载体出品公司: | -- |
| 空载体大小: | 5729 bp |
| 完整质粒大小: | 5080 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 载体抗性: | 氨苄青霉素，100μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Igkc |
| 物种来源: | M.musculus（小鼠） |
| 插入大小: | 321 bp |
| 基因突变: | -- |
| GenBank编号 | LR588433 |
| 启动子: | 人巨细胞病毒IE1基因启动子 |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | AgeI(未破坏) |
| 3’克隆位点: | PmlI (破坏) |
| 5’测序引物 | GCTTCGTTAGAACGCGGCTAC |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | AbVec2.0-mIgkc was a gift from Hedda Wardemann (Addgene plasmid # 127157 ; http://n2t.net/addgene:127157 ; RRID:Addgene_127157) |
| 参考文献部分: | ALDH4A1 is an atherosclerosis auto-antigen targeted by protective antibodies. Lorenzo C, Delgado P, Busse CE, Sanz-Bravo A, Martos-Folgado I, Bonzon-Kulichenko E, Ferrarini A, Gonzalez-Valdes IB, Mur SM, Roldan-Montero R, Martinez-Lopez D, Martin-Ventura JL, Vazquez J, Wardemann H, Ramiro AR. Nature. 2021 Jan;589(7841):287-292. doi: 10.1038/s41586-020-2993-2. Epub 2020 Dec 2. 10.1038/s41586-020-2993-2 PubMed 33268892 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS AbVec2.0-mIgkc 5080 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Expression of immunoglobulin light chains in mammalian cells, mouse
(C57BL/6), kappa constant region.
ACCESSION .
VERSION .
KEYWORDS AbVec2.0-mIgkc
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5080)
TITLE Mouse immunoglobulin expression vectors
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 5080)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5080
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 106..127
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 142..172
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 180..196
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 185..207
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 204..220
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 204..220
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
enhancer 249..628
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 629..832
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 783..803
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 829..853
/label=LNCX
/note="Human CMV promoter, forward primer"
promoter 1063..1081
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind 1063..1080
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind 1094..1113
/label=pMT2-F
/note="Synthetic intron, forward primer"
regulatory 1615..1624
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
polyA_signal 1628..1762
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1665..1684)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 1719..1738
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
promoter 1831..2188
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(1854..1874)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 2039..2174
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 2101..2120
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(2208..2225)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(2208..2224)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2217..2239)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 2437..2892
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2524..2543)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 2734..2755
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(2909..2928)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 3028..3050
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(3088..3106)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 3174..3278
/gene="bla"
/label=AmpR promoter
CDS 3279..4139
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(3497..3516)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 4310..4898
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 4799..4818
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 5052..5069
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat ccaactggca
61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
181 tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacga attaattcga
241 gctcgcccga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
301 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
361 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
421 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
481 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
541 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
601 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
661 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
721 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
781 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt
841 gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg
901 ggaccgatcc agcctccgcg gccgggaacg gtgcattgga acgcggattc cccgtgccaa
961 gagtgacgta agtaccgcct atagagtcta taggcccacc cccttggctt cgttagaacg
1021 cggctacaat taatacataa ccttatgtat catacacata cgatttaggt gacactatag
1081 aataacatcc actttgcctt tctctccaca ggtgtccact cccaggtcca actgcacctc
1141 ggttctatcg attgaattcc accatgggat ggtcatgtat catccttttt ctagtagcaa
1201 ctgcaaccgg ttgactaact agccacgtgc tgatgctgca ccaactgtaa gcatcttccc
1261 accatccagt gagcagttaa catctggagg tgcctcagtc gtgtgcttct tgaacaactt
1321 ctaccccaaa gacatcaatg tcaagtggaa gattgatggc agtgaacgac aaaatggcgt
1381 cctgaacagt tggactgatc aggacagcaa agacagcacc tacagcatga gcagcaccct
1441 cacgttgacc aaggacgagt atgaacgaca taacagctat acctgtgagg ccactcacaa
1501 gacatcaact tcacccattg tcaagagctt caacaggaat gagtgttaga gacaaaggtc
1561 ctgagacgcc accaccagct ccccagctcc atcctatctt cccttctaag cttggccgcc
1621 atggcccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
1681 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
1741 tgtatcttat catgtctgga tcgggaatta attcggcgca gcaccatggc ctgaaataac
1801 ctctgaaaga ggaacttggt taggtacctt ctgaggcgga aagaaccatc tgtggaatgt
1861 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
1921 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
1981 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
2041 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
2101 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
2161 cttttttgga ggcctaggct tttgcaaaaa gctgttaaca gcttggcact ggccgtcgtt
2221 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
2281 ccccctttcg ccagttggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
2341 ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc
2401 ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa
2461 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
2521 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
2581 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
2641 aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
2701 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
2761 cactcaaccc tatctcgggc tattcttttg atttataagg gattttgccg atttcggcct
2821 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
2881 cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
2941 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
3001 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
3061 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
3121 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
3181 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
3241 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
3301 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
3361 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
3421 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
3481 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
3541 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
3601 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
3661 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
3721 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
3781 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
3841 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
3901 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
3961 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
4021 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
4081 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
4141 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
4201 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
4261 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
4321 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
4381 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
4441 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
4501 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
4561 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
4621 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
4681 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
4741 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
4801 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
4861 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
4921 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
4981 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
5041 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
//
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