| 载体名称: | pGADT7-Rec,pGADT7Rec |
|---|---|
| 质粒类型: | 酵母系列，酵母单杂交载体 |
| 高拷贝/低拷贝: | -- |
| 复制子: | pUC |
| 启动子: | ADH1 |
| 载体大小: | 8058bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | LEU2 |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 酵母细胞 |
| 备注: | -- |
| 产品目录号: | -- |
买家导航
pGADT7-Rec载体 pGADT7Rec质粒基本信息
pGADT7-Rec质粒图谱 pGADT7Rec载体图谱和pGADT7-Rec载体序列 pGADT7Rec质粒序列多克隆位点信息
pGADT7-Rec质粒 pGADT7Rec载体简介
pGADT7-Rec质粒序列 pGADT7Rec载体序列
LOCUS pGADT7-Rec 8058 bp ds-DNA circular SYN 19-OCT-2017
DEFINITION High-copy yeast vector for fusing a cDNA to the GAL4 activation
domain by in vivo recombination.
ACCESSION .
VERSION .
KEYWORDS pGADT7-Rec
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8058)
AUTHORS Clontech (TaKaRa)
TITLE Direct Submission
JOURNAL Exported Saturday, December 7, 2019 from SnapGene Viewer 3.1.4
http://www.snapgene.com
COMMENT Linearize with SmaI.
FEATURES Location/Qualifiers
source 1..8058
/organism="synthetic DNA construct"
/lab_host="Saccharomyces cerevisiae"
/mol_type="other DNA"
promoter 771..1475
/gene="S. cerevisiae ADH1"
/note="ADH1 promoter"
/note="promoter for alcohol dehydrogenase 1"
CDS 1491..1493
/codon_start=1
/product="start codon"
/note="ATG"
/translation="M"
CDS 1521..1541
/codon_start=1
/product="nuclear localization signal of SV40 large T
antigen"
/note="SV40 NLS"
/translation="PKKKRKV"
CDS 1557..1898
/codon_start=1
/gene="Saccharomyces cerevisiae GAL4 (truncated)"
/product="activation domain of the GAL4 transcriptional
activator"
/note="GAL4 activation domain"
/translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
EDTPPNPKKE"
promoter 1904..1922
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 1935..1937
/codon_start=1
/product="in vitro start codon"
/note="ATG"
/translation="M"
CDS 1941..1967
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/note="HA"
/translation="YPYDVPDYA"
misc_feature 2000..2035
/label=SMART III Oligonucleotide Sequence
/note="SMART III"
/note="matches the SMART III Oligonucleotide for cDNA
synthesis"
misc_feature 2046..2070
/label=CDS III Sequence
/note="CDS III"
/note="matches the CDS III Primer for cDNA synthesis"
terminator 2486..2673
/gene="S. cerevisiae ADH1"
/note="ADH1 terminator"
/note="transcription terminator for alcohol dehydrogenase
1"
CDS complement(2790..3884)
/codon_start=1
/gene="S. cerevisiae LEU2"
/product="3-isopropylmalate dehydrogenase, required for
leucine biosynthesis"
/note="LEU2"
/note="yeast auxotrophic marker"
/translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
IGGAAIEATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
DLGGSNSTTEVGDAVAEEVKKILA"
promoter complement(3885..4290)
/gene="S. cerevisiae LEU2"
/note="LEU2 promoter"
rep_origin complement(4885..5473)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5644..6504)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(6505..6609)
/gene="bla"
/note="AmpR promoter"
rep_origin 6891..8055
/note="2u ori"
/note="yeast 2u plasmid origin of replication"
ORIGIN
1 tgcatgcctg caggtcgaga tccgggatcg aagaaatgat ggtaaatgaa ataggaaatc
61 aaggagcatg aaggcaaaag acaaatataa gggtcgaacg aaaaataaag tgaaaagtgt
121 tgatatgatg tatttggctt tgcggcgccg aaaaaacgag tttacgcaat tgcacaatca
181 tgctgactct gtggcggacc cgcgctcttg ccggcccggc gataacgctg ggcgtgaggc
241 tgtgcccggc ggagtttttt gcgcctgcat tttccaaggt ttaccctgcg ctaaggggcg
301 agattggaga agcaataaga atgccggttg gggttgcgat gatgacgacc acgacaactg
361 gtgtcattat ttaagttgcc gaaagaacct gagtgcattt gcaacatgag tatactagaa
421 gaatgagcca agacttgcga gacgcgagtt tgccggtggt gcgaacaata gagcgaccat
481 gaccttgaag gtgagacgcg cataaccgct agagtacttt gaagaggaaa cagcaatagg
541 gttgctacca gtataaatag acaggtacat acaacactgg aaatggttgt ctgtttgagt
601 acgctttcaa ttcatttggg tgtgcacttt attatgttac aatatggaag ggaactttac
661 acttctccta tgcacatata ttaattaaag tccaatgcta gtagagaagg ggggtaacac
721 ccctccgcgc tcttttccga tttttttcta aaccgtggaa tatttcggat atccttttgt
781 tgtttccggg tgtacaatat ggacttcctc ttttctggca accaaaccca tacatcggga
841 ttcctataat accttcgttg gtctccctaa catgtaggtg gcggagggga gatatacaat
901 agaacagata ccagacaaga cataatgggc taaacaagac tacaccaatt acactgcctc
961 attgatggtg gtacataacg aactaatact gtagccctag acttgatagc catcatcata
1021 tcgaagtttc actacccttt ttccatttgc catctattga agtaataata ggcgcatgca
1081 acttcttttc tttttttttc ttttctctct cccccgttgt tgtctcacca tatccgcaat
1141 gacaaaaaaa tgatggaaga cactaaagga aaaaattaac gacaaagaca gcaccaacag
1201 atgtcgttgt tccagagctg atgaggggta tctcgaagca cacgaaactt tttccttcct
1261 tcattcacgc acactactct ctaatgagca acggtatacg gccttccttc cagttacttg
1321 aatttgaaat aaaaaaaagt ttgctgtctt gctatcaagt ataaatagac ctgcaattat
1381 taatcttttg tttcctcgtc attgttctcg ttccctttct tccttgtttc tttttctgca
1441 caatatttca agctatacca agcatacaat caactccaag ctttgcaaag atggataaag
1501 cggaattaat tcccgagcct ccaaaaaaga agagaaaggt cgaattgggt accgccgcca
1561 attttaatca aagtgggaat attgctgata gctcattgtc cttcactttc actaacagta
1621 gcaacggtcc gaacctcata acaactcaaa caaattctca agcgctttca caaccaattg
1681 cctcctctaa cgttcatgat aacttcatga ataatgaaat cacggctagt aaaattgatg
1741 atggtaataa ttcaaaacca ctgtcacctg gttggacgga ccaaactgcg tataacgcgt
1801 ttggaatcac tacagggatg tttaatacca ctacaatgga tgatgtatat aactatctat
1861 tcgatgatga agatacccca ccaaacccaa aaaaagagat ctttaatacg actcactata
1921 gggcgagcgc cgccatggag tacccatacg acgtaccaga ttacgctcat atggccatgg
1981 aggccagtga attccaccca agcagtggta tcaacgcaga gtggccatta tggcccggga
2041 aaaaacatgt cggccgcctc ggcctctaga gggtgggcat cgatacggga tccatcgagc
2101 tcgagctgca gatgaatcgt agatactgaa aaaccccgca agttcacttc aactgtgcat
2161 cgtgcaccat ctcaatttct ttcatttata catcgttttg ccttctttta tgtaactata
2221 ctcctctaag tttcaatctt ggccatgtaa cctctgatct atagaatttt ttaaatgact
2281 agaattaatg cccatctttt ttttggacct aaattcttca tgaaaatata ttacgagggc
2341 ttattcagaa gctttggact tcttcgccag aggtttggtc aagtctccaa tcaaggttgt
2401 cggcttgtct accttgccag aaatttacga aaagatggaa aagggtcaaa tcgttggtag
2461 atacgttgtt gacacttcta aataagcgaa tttcttatga tttatgattt ttattattaa
2521 ataagttata aaaaaaataa gtgtatacaa attttaaagt gactcttagg ttttaaaacg
2581 aaaattctta ttcttgagta actctttcct gtaggtcagg ttgctttctc aggtatagca
2641 tgaggtcgct cttattgacc acacctctac cggccggtcg aaattcccct accctatgaa
2701 catattccat tttgtaattt cgtgtcgttt ctattatgaa tttcatttat aaagtttatg
2761 tacaaatatc ataaaaaaag agaatctttt taagcaagga ttttcttaac ttcttcggcg
2821 acagcatcac cgacttcggt ggtactgttg gaaccaccta aatcaccagt tctgatacct
2881 gcatccaaaa cctttttaac tgcatcttca atggccttac cttcttcagg caagttcaat
2941 gacaatttca acatcattgc agcagacaag atagtggcga tagggttgac cttattcttt
3001 ggcaaatctg gagcagaacc gtggcatggt tcgtacaaac caaatgcggt gttcttgtct
3061 ggcaaagagg ccaaggacgc agatggcaac aaacccaagg aacctgggat aacggaggct
3121 tcatcggaga tgatatcacc aaacatgttg ctggtgatta taataccatt taggtgggtt
3181 gggttcttaa ctaggatcat ggcggcagaa tcaatcaatt gatgttgaac cttcaatgta
3241 ggaaattcgt tcttgatggt ttcctccaca gtttttctcc ataatcttga agaggccaaa
3301 acattagctt tatccaagga ccaaataggc aatggtggct catgttgtag ggccatgaaa
3361 gcggccattc ttgtgattct ttgcacttct ggaacggtgt attgttcact atcccaagcg
3421 acaccatcac catcgtcttc ctttctctta ccaaagtaaa tacctcccac taattctctg
3481 acaacaacga agtcagtacc tttagcaaat tgtggcttga ttggagataa gtctaaaaga
3541 gagtcggatg caaagttaca tggtcttaag ttggcgtaca attgaagttc tttacggatt
3601 tttagtaaac cttgttcagg tctaacacta cctgtacccc atttaggacc acccacagca
3661 cctaacaaaa cggcatcagc cttcttggag gcttccagcg cctcatctgg aagtgggaca
3721 cctgtagctt cgatagcagc accaccaatt aaatgatttt cgaaatcgaa cttgacattg
3781 gaacgaacat cagaaatagc tttaagaacc ttaatggctt cggctgtgat ttcttgacca
3841 acgtggtcac ctggcaaaac gacgatcttc ttaggggcag acattagaat ggtatatcct
3901 tgaaatatat atatatattg ctgaaatgta aaaggtaaga aaagttagaa agtaagacga
3961 ttgctaacca cctattggaa aaaacaatag gtccttaaat aatattgtca acttcaagta
4021 ttgtgatgca agcatttagt catgaacgct tctctattct atatgaaaag ccggttccgg
4081 cgctctcacc tttccttttt ctcccaattt ttcagttgaa aaaggtatat gcgtcaggcg
4141 acctctgaaa ttaacaaaaa atttccagtc atcgaatttg attctgtgcg atagcgcccc
4201 tgtgtgttct cgttatgttg aggaaaaaaa taatggttgc taagagattc gaactcttgc
4261 atcttacgat acctgagtat tcccacagtt gggggatctc gactctagct agaggatcaa
4321 ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
4381 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
4441 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
4501 ataacttcgt ataatgtatg ctatacgaag ttattaggtc tgaagaggag tttacgtcca
4561 gccaagctag cttggctgca ggtcgagcgg ccgcgatccg gaacccttaa tataacttcg
4621 tataatgtat gctatacgaa gttatcagct gcattaatga atcggccaac gcgcggggag
4681 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
4741 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
4801 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
4861 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
4921 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
4981 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
5041 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
5101 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
5161 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
5221 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
5281 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
5341 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
5401 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
5461 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
5521 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
5581 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
5641 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
5701 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
5761 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
5821 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
5881 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
5941 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
6001 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
6061 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
6121 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
6181 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
6241 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
6301 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
6361 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
6421 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
6481 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
6541 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
6601 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
6661 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
6721 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
6781 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
6841 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata acgcatttaa
6901 gcataaacac gcactatgcc gttcttctca tgtatatata tatacaggca acacgcagat
6961 ataggtgcga cgtgaacagt gagctgtatg tgcgcagctc gcgttgcatt ttcggaagcg
7021 ctcgttttcg gaaacgcttt gaagttccta ttccgaagtt cctattctct agctagaaag
7081 tataggaact tcagagcgct tttgaaaacc aaaagcgctc tgaagacgca ctttcaaaaa
7141 accaaaaacg caccggactg taacgagcta ctaaaatatt gcgaataccg cttccacaaa
7201 cattgctcaa aagtatctct ttgctatata tctctgtgct atatccctat ataacctacc
7261 catccacctt tcgctccttg aacttgcatc taaactcgac ctctacattt tttatgttta
7321 tctctagtat tactctttag acaaaaaaat tgtagtaaga actattcata gagtgaatcg
7381 aaaacaatac gaaaatgtaa acatttccta tacgtagtat atagagacaa aatagaagaa
7441 accgttcata attttctgac caatgaagaa tcatcaacgc tatcactttc tgttcacaaa
7501 gtatgcgcaa tccacatcgg tatagaatat aatcggggat gcctttatct tgaaaaaatg
7561 cacccgcagc ttcgctagta atcagtaaac gcgggaagtg gagtcaggct ttttttatgg
7621 aagagaaaat agacaccaaa gtagccttct tctaacctta acggacctac agtgcaaaaa
7681 gttatcaaga gactgcatta tagagcgcac aaaggagaaa aaaagtaatc taagatgctt
7741 tgttagaaaa atagcgctct cgggatgcat ttttgtagaa caaaaaagaa gtatagattc
7801 tttgttggta aaatagcgct ctcgcgttgc atttctgttc tgtaaaaatg cagctcagat
7861 tctttgtttg aaaaattagc gctctcgcgt tgcatttttg ttttacaaaa atgaagcaca
7921 gattcttcgt tggtaaaata gcgctttcgc gttgcatttc tgttctgtaa aaatgcagct
7981 cagattcttt gtttgaaaaa ttagcgctct cgcgttgcat ttttgttcta caaaatgaag
8041 cacagatgct tcgttgct
//
