pRL-SV40

价格:1000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Promega
载体名称: pRL-SV40
质粒类型: 哺乳细胞表达;启动子增强子报告系统
高拷贝/低拷贝: --
启动子: SV40
克隆方法: 多克隆位点,限制性内切酶
载体大小: 3705bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄青霉素(Ampicillin)
筛选标记: --
备注: pRL-SV40是荧光素酶报告载体,含有SV40增强子。
产品目录号: E2231
稳定性: 瞬表达
组成型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pRL-SV40载体图谱



pRL-SV40载体特征
pRL-SV40载体图谱2



载体简介

载体序列

LOCUS       pRL-SV40	3705 bp 	DNA	SYN
DEFINITION  pRL-SV40
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="pRL-SV40"
                     /mol_type="other DNA"
     misc_feature    complement(85..105)
                     /label="pBABE_3_primer"
     misc_feature    complement(91..306)
                     /label="SV40_enhancer"
     promoter        103..371
                     /label="SV40_promoter"
     rep_origin      270..347
                     /label="SV40_origin"
     misc_feature    332..351
                     /label="SV40pro_F_primer"
     misc_feature    489..621
                     /label="chim_int"
     promoter        666..684
                     /label="T7_promoter"
     CDS             694..1629
                     /label="ORF frame 1"

     terminator      complement(1657..1776)
                     /label="SV40_PA_terminator"

     misc_feature    complement(1671..1690)
                     /label="EBV_rev_primer"

     promoter        1949..1977
                     /label="AmpR_promoter"

     gene            2019..2879
                     /label="Ampicillin"
                     /gene="Ampicillin"

     CDS             2019..2879
                     /label="ORF frame 3"

     rep_origin      3034..3653
                     /label="pBR322_origin"

ORIGIN
    1 AGATCTGCGC AGCACCATGG CCTGAAATAA CCTCTGAAAG AGGAACTTGG TTAGGTACCT
   61 TCTGAGGCGG AAAGAACCAG CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG
  121 GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG
  181 GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG
  241 CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC
  301 ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG
  361 CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA
  421 AGCTTGATTC TTCTGACACA ACAGTCTCGA ACTTAAGCTG CAGAAGTTGG TCGTGAGGCA
  481 CTGGGCAGGT AAGTATCAAG GTTACAAGAC AGGTTTAAGG AGACCAATAG AAACTGGGCT
  541 TGTCGAGACA GAGAAGACTC TTGCGTTTCT GATAGGCACC TATTGGTCTT ACTGACATCC
  601 ACTTTGCCTT TCTCTCCACA GGTGTCCACT CCCAGTTCAA TTACAGCTCT TAAGGCTAGA
  661 GTACTTAATA CGACTCACTA TAGGCTAGCC ACCATGACTT CGAAAGTTTA TGATCCAGAA
  721 CAAAGGAAAC GGATGATAAC TGGTCCGCAG TGGTGGGCCA GATGTAAACA AATGAATGTT
  781 CTTGATTCAT TTATTAATTA TTATGATTCA GAAAAACATG CAGAAAATGC TGTTATTTTT
  841 TTACATGGTA ACGCGGCCTC TTCTTATTTA TGGCGACATG TTGTGCCACA TATTGAGCCA
  901 GTAGCGCGGT GTATTATACC AGACCTTATT GGTATGGGCA AATCAGGCAA ATCTGGTAAT
  961 GGTTCTTATA GGTTACTTGA TCATTACAAA TATCTTACTG CATGGTTTGA ACTTCTTAAT
 1021 TTACCAAAGA AGATCATTTT TGTCGGCCAT GATTGGGGTG CTTGTTTGGC ATTTCATTAT
 1081 AGCTATGAGC ATCAAGATAA GATCAAAGCA ATAGTTCACG CTGAAAGTGT AGTAGATGTG
 1141 ATTGAATCAT GGGATGAATG GCCTGATATT GAAGAAGATA TTGCGTTGAT CAAATCTGAA
 1201 GAAGGAGAAA AAATGGTTTT GGAGAATAAC TTCTTCGTGG AAACCATGTT GCCATCAAAA
 1261 ATCATGAGAA AGTTAGAACC AGAAGAATTT GCAGCATATC TTGAACCATT CAAAGAGAAA
 1321 GGTGAAGTTC GTCGTCCAAC ATTATCATGG CCTCGTGAAA TCCCGTTAGT AAAAGGTGGT
 1381 AAACCTGACG TTGTACAAAT TGTTAGGAAT TATAATGCTT ATCTACGTGC AAGTGATGAT
 1441 TTACCAAAAA TGTTTATTGA ATCGGACCCA GGATTCTTTT CCAATGCTAT TGTTGAAGGT
 1501 GCCAAGAAGT TTCCTAATAC TGAATTTGTC AAAGTAAAAG GTCTTCATTT TTCGCAAGAA
 1561 GATGCACCTG ATGAAATGGG AAAATATATC AAATCGTTCG TTGAGCGAGT TCTCAAAAAT
 1621 GAACAATAAT TCTAGAGCGG CCGCTTCGAG CAGACATGAT AAGATACATT GATGAGTTTG
 1681 GACAAACCAC AACTAGAATG CAGTGAAAAA AATGCTTTAT TTGTGAAATT TGTGATGCTA
 1741 TTGCTTTATT TGTAACCATT ATAAGCTGCA ATAAACAAGT TAACAACAAC AATTGCATTC
 1801 ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG TAAAACCTCT
 1861 ACAAATGTGG TAAAATCGAT AAGGATCCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA
 1921 CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC
 1981 CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG
 2041 TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC
 2101 TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG
 2161 ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA
 2221 GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC
 2281 AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG
 2341 AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA
 2401 GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG
 2461 CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA
 2521 ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT
 2581 TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT
 2641 GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT
 2701 TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG
 2761 GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA
 2821 TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC
 2881 TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA
 2941 AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT
 3001 TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT
 3061 TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT
 3121 GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC
 3181 AGATACCAAA TACTGTTCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG
 3241 TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG
 3301 ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT
 3361 CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC
 3421 TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG
 3481 ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG
 3541 GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT
 3601 TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT
 3661 TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGGC TCGAC
//

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