pWZ403

LOCUS        pWZ403        7489 bp DNA     circular SYN 25-AUG-2024
DEFINITION  mSwAP-In marker cassette 1 with mScarlet-PuroTK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7489)
  AUTHORS   Zhang W, Golynker I, Brosh R, Fajardo A, Zhu Y, Wudzinska AM, 
            Ordonez R, Ribeiro-Dos-Santos AM, Carrau L, Damani-Yokota P, Yeung 
            ST, Khairallah C, Vela Gartner A, Chalhoub N, Huang E, Ashe HJ, 
            Khanna KM, Maurano MT, Kim SY, tenOever BR, Boeke JD
  TITLE     Mouse genome rewriting and tailoring of three important disease 
            loci.
  JOURNAL   Nature. 2023 Nov 1. doi: 10.1038/s41586-023-06675-4.
  PUBMED    37914927
REFERENCE   2  (bases 1 to 7489)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 25, 2024 from SnapGene Server 7.0.3
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2023 Nov 1. doi: 10.1038/s41586-023-06675-4."
FEATURES             Location/Qualifiers
     source          1..7489
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        239..257
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     239..256
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        460..1638
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          691..1629
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1586..1606
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1639..2334
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(1772..1790)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2040..2059
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             2344..2400
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2404..3000
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2404..2423)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2900..2920
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3001..3996
                     /codon_start=1
                     /product="truncated herpes simplex virus thymidine kinase 
                     (Salomon et al., 1995)"
                     /label=Delta-TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRV
                     LGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGS
                     SHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGAL
                     PEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQCGGSWREDWGQLSGTAV
                     PPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRSMHVFI
                     LDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     polyA_signal    4108..4229
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4145..4164)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4199..4218
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4377..4396)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4378..4396)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4403..4420)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4403..4419)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4412..4434)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             4557..4859
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4738..4757
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             5208..6002
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5208..6002
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5262..5281)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5872..5891
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     regulatory      6202..6211
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6208..6582
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     rep_origin      6720..7308
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7209..7228
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7462..7479
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat
      241 ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca
      301 ctagtaacgg ccgccagtgt gctggaattc gcccttgcta ctgccgtgta tcgtattgtg
      361 ggcgtactgc cgtgtatcgt attgtggact ctgaggtaaa tccacaatgc agtgcttcag
      421 ccgctacccc gaccacatga agcagcacga cttcttcaag gctccggtgc ccgtcagtgg
      481 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
      541 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
      601 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
      661 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
      721 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
      781 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
      841 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
      901 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
      961 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     1021 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     1081 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     1141 cgggggtagt ctcaagctgg ccggcctgct ctggtgccta gtctcgcgcc gccgtgtatc
     1201 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     1261 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     1321 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     1381 gactccacgg agtaccgggc gctgtccagg cacctcgatt agttctcgag cttttggagt
     1441 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     1501 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     1561 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     1621 ccatttcagg tgtcgtgaat ggtgagcaag ggcgaggcag tgatcaagga gttcatgcgg
     1681 ttcaaggtgc acatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc
     1741 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc
     1801 ctgcccttct cctgggacat cctgtcccct cagttcatgt acggctccag ggccttcatc
     1861 aagcaccccg ccgacatccc cgactactat aagcagtcct tccccgaggg cttcaagtgg
     1921 gagcgcgtga tgaacttcga ggacggcggc gccgtgaccg tgacccagga cacctccctg
     1981 gaggacggca ccctgatcta caaggtgaag ctccgcggca ccaacttccc tcctgacggc
     2041 cccgtaatgc agaagaagac aatgggctgg gaagcgtcca ccgagcggtt gtaccccgag
     2101 gacggcgtgc tgaagggcga cattaagatg gccctgcgcc tgaaggacgg cggccgctac
     2161 ctggcggact tcaagaccac ctacaaggcc aagaagcccg tgcagatgcc cggcgcctac
     2221 aacgtcgacc gcaagttgga catcacctcc cacaacgagg actacaccgt ggtggaacag
     2281 tacgaacgct ccgagggccg ccactccacc ggcggcatgg acgagctgta caaggggtca
     2341 ggcgcgacta acttctccct gctgaagcag gcaggcgacg tggaggaaaa cccaggcccg
     2401 agcatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
     2461 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg
     2521 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
     2581 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
     2641 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
     2701 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
     2761 gagcccgcgt ggttcctggc caccgtcggc gtgtcgcccg accaccaggg caagggtctg
     2821 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
     2881 ctggaaacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
     2941 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
     3001 atgcccacgc tactgcgggt ttatatagac ggtccccacg ggatggggaa aaccaccacc
     3061 acgcaactgc tggtggccct gggttcgcgc gacgatatcg tctacgtacc cgagccgatg
     3121 acttactggc gggtgctggg ggcttccgag acaatcgcga acatctacac cacacaacac
     3181 cgcctcgacc agggtgagat atcggccggg gacgcggcgg tggtaatgac aagcgcccag
     3241 ataacaatgg gcatgcctta tgccgtgacc gacgccgttc tggctcctca tatcgggggg
     3301 gaggctggga gctcacatgc cccgcccccg gccctcaccc tcatcttcga ccgccatccc
     3361 atcgccgccc tcctgtgcta cccggccgcg cggtacctta tgggcagcat gaccccccag
     3421 gccgtgctgg cgttcgtggc cctcatcccg ccgaccttgc ccggcaccaa catcgtgctt
     3481 ggggcccttc cggaggacag acacatcgac cgcctggcca aacgccagcg ccccggcgag
     3541 cggctggacc tggctatgct ggctgcgatt cgccgcgttt acgggctact tgccaatacg
     3601 gtgcggtatc tgcagtgcgg cgggtcgtgg cgggaggact ggggacagct ttcggggacg
     3661 gccgtgccgc cccagggtgc cgagccccag agcaacgcgg gcccacgacc ccatatcggg
     3721 gacacgttat ttaccctgtt tcgggccccc gagttgctgg cccccaacgg cgacctgtat
     3781 aacgtgtttg cctgggcctt ggacgtcttg gccaaacgcc tccgttccat gcacgtcttt
     3841 atcctggatt acgaccaatc gcccgccggc tgccgggacg ccctgctgca acttacctcc
     3901 gggatggtcc agacccacgt caccaccccc ggctccatac cgacgatatg cgacctggcg
     3961 cgcacgtttg cccgggagat gggggaggct aactgaagct tgatcataat cagccatacc
     4021 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     4081 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     4141 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4201 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tcgaggggtg agagagaccc
     4261 ggtacgatac acggcagtac gcccacaata cgatacacgg cagtagcaag ggcgaattct
     4321 gcagatatcc atcacactgg cggccgctcg agcatgcatc tagagggccc aattcgccct
     4381 atagtgagtc gtattacaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     4441 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     4501 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctatac gtacggcagt
     4561 ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg
     4621 atattattga cacgccgggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt
     4681 cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca
     4741 tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc
     4801 tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa
     4861 tgtcaggcat gagattatca aaaaggatct tcacctagat ccttttcacg tagaaagcca
     4921 gtccgcagaa acggtgctga ccccggatga atgtcagcta ctgggctatc tggacaaggg
     4981 aaaacgcaag cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag
     5041 actgggcggt tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta
     5101 aggttgggaa gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc
     5161 gcaggggatc aagctctgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     5221 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     5281 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     5341 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag
     5401 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     5461 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     5521 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     5581 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     5641 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     5701 tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg
     5761 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     5821 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     5881 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     5941 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     6001 gaattattaa cgcttacaat ttcctgatgc ggtattttct ccttacgcat ctgtgcggta
     6061 tttcacaccg catcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     6121 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     6181 caataatagc acgtgaggag ggccaccatg gccaagttga ccagtgccgt tccggtgctc
     6241 accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg
     6301 gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc
     6361 gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg
     6421 gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg
     6481 ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg
     6541 gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct aaaacttcat
     6601 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     6661 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     6721 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     6781 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     6841 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     6901 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     6961 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     7021 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     7081 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     7141 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     7201 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     7261 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     7321 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     7381 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     7441 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaag
//