pAd/PL-DEST

价格:2500元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Invitrogen
载体名称: pAd/PL-DEST
质粒类型: 腺病毒表达载体;Gateway系统
高拷贝/低拷贝: 高拷贝
启动子: 无启动子
克隆方法: Gateway
载体大小: 34864 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: 无标签
载体抗性: 青霉素
筛选标记: --
备注: 启动子功能研究
产品目录号: V494-20
稳定性: 稳表达
组成型: 组成型
病毒/非病毒: 腺病毒

载体质粒图谱和多克隆位点信息

pAd-PL-DEST载体图谱



pAd-PL-DEST多克隆位点

载体简介

The pAd/PL-DEST Gateway Vector Kit contains the Gateway-adapted ViraPower adenoviral expression vector, pAd/PL-DEST vector for easy recombination-based cloning and adenoviral-based, transient expression of a target gene in dividing and non-dividing mammalian cells. The vector allows generation of an adenovirus containing the target gene where expression is driven by a promoter of choice. Alternatively, the vector may also be used to express small RNA molecules from their appropriate promoters.

Advantages
 High efficiency and rapid recombination cloning
 Produces high titer adenoviral stocks
 Efficient delivery of the gene to dividing and non-dividing mammalian cells in vitro or in vivo
 Allows gene of interest to be controlled by a promoter of choice
 Produces replication-incompetent virus for enhanced biosafety of the system
 Amenable for use in high-throughput applications

Key Features
 Gateway Technology for efficient and rapid cloning
 Promoterless vector that allows gene of interest to be controlled by a promoter of choice 
 Human Ad5 sequences (ΔE3) and Viral Inverted Terminal Repeats (ITRs) for packaging of the expression construct into virions
 Ampicillin selection marker

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