| 质粒名称: | rAAV2/MG1.2 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 184541 |
| 存储实验室: | Minmin Luo |
|
载体骨架基本信息 |
|
| 载体名称: | AAV helper vector |
| 载体出品公司: | Clontech |
| 完整质粒大小: | 7283bp |
| 载体类型: | AAV ; helper vector for packaging |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | AAV-MG1.2 capsid |
| 物种来源: | Synthetic |
| 插入大小: | 2232bp |
| 基因突变: | changes on AAV9 capsid: K449R, Q588T; insertion of EPPKTTS between T588 and A589 |
|
文献引用方法 |
|
| 材料和方法部分: |
rAAV2/MG1.2 was a gift from Minmin Luo (Addgene plasmid # 184541 ; http://n2t.net/addgene:184541 ; RRID:Addgene_184541)
|
| 参考文献部分: | Directed evolution of adeno-associated virus for efficient gene delivery to microglia. Lin R, Zhou Y, Yan T, Wang R, Li H, Wu Z, Zhang X, Zhou X, Zhao F, Zhang L, Li Y, Luo M. Nat Methods. 2022 Jul 25. pii: 10.1038/s41592-022-01547-7. doi: 10.1038/s41592-022-01547-7. 10.1038/s41592-022-01547-7 PubMed 35879607 |
买家导航
rAAV2/MG1.2载体质粒基本信息
rAAV2/MG1.2质粒图谱载体图谱和rAAV2/MG1.2载体序列质粒序列多克隆位点信息
rAAV2/MG1.2质粒载体简介
rAAV2/MG1.2质粒序列载体序列
LOCUS rAAV2/MG1.2 7283 bp DNA circular SYN 14-APR-2025
DEFINITION AAV packaging plasmid, expressing Rep2 and AAV-MG1.2 capsid.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7283)
AUTHORS Lin R, Zhou Y, Yan T, Wang R, Li H, Wu Z, Zhang X, Zhou X, Zhao F,
Zhang L, Li Y, Luo M
TITLE Directed evolution of adeno-associated virus for efficient gene
delivery to microglia.
JOURNAL Nat Methods. 2022 Jul 25. pii: 10.1038/s41592-022-01547-7. doi:
10.1038/s41592-022-01547-7.
PUBMED 35879607
REFERENCE 2 (bases 1 to 7283)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nat
Methods. 2022 Jul 25. pii: 10.1038/s41592-022-01547-7. doi:
10.1038/s41592-022-01547-7."
COMMENT Sequence Label: rAAV2/MG1.2
FEATURES Location/Qualifiers
source 1..7283
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 11..33
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 25..42
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 26..42
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 52..71
/label=T7
/note="T7 promoter, forward primer"
promoter 52..70
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 99..115
/label=pBluescriptKS
/note="For pBluescript vector"
primer_bind 100..116
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
CDS 4126..4137
/codon_start=1
/product="WELQut protease recognition and cleavage site"
/label=WELQut site
/translation="WELQ"
primer_bind complement(4521..4541)
/label=T3
/note="T3 promoter, forward primer"
promoter complement(4521..4539)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(4560..4576)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4560..4576)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(4573..4595)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4584..4600
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(join(4608..4614,4615..4632,4633..4638))
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4653..4674
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(4791..4808)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4962..5550)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5042..5061)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(5721..6512,6513..6581))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6344..6363
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6582..6686)
/gene="bla"
/label=AmpR promoter
rep_origin complement(6712..7167)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6849..6870)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 7061..7080
/label=F1ori-R
/note="F1 origin, reverse primer"
ORIGIN
1 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac
61 tcactatagg gcgaattggg taccgggccc cccctcgatc gaggtcgacg gtatcggggg
121 agctcgcagg gtctccattt tgaagcggga ggtttgaacg cgcagccgcc atgccggggt
181 tttacgagat tgtgattaag gtccccagcg accttgacga gcatctgccc ggcatttctg
241 acagctttgt gaactgggtg gccgagaagg aatgggagtt gccgccagat tctgacatgg
301 atctgaatct gattgagcag gcacccctga ccgtggccga gaagctgcag cgcgactttc
361 tgacggaatg gcgccgtgtg agtaaggccc cggaggctct tttctttgtg caatttgaga
421 agggagagag ctacttccac atgcacgtgc tcgtggaaac caccggggtg aaatccatgg
481 ttttgggacg tttcctgagt cagattcgcg aaaaactgat tcagagaatt taccgcggga
541 tcgagccgac tttgccaaac tggttcgcgg tcacaaagac cagaaatggc gccggaggcg
601 ggaacaaggt ggtggatgag tgctacatcc ccaattactt gctccccaaa acccagcctg
661 agctccagtg ggcgtggact aatatggaac agtatttaag cgcctgtttg aatctcacgg
721 agcgtaaacg gttggtggcg cagcatctga cgcacgtgtc gcagacgcag gagcagaaca
781 aagagaatca gaatcccaat tctgatgcgc cggtgatcag atcaaaaact tcagccaggt
841 acatggagct ggtcgggtgg ctcgtggaca aggggattac ctcggagaag cagtggatcc
901 aggaggacca ggcctcatac atctccttca atgcggcctc caactcgcgg tcccaaatca
961 aggctgcctt ggacaatgcg ggaaagatta tgagcctgac taaaaccgcc cccgactacc
1021 tggtgggcca gcagcccgtg gaggacattt ccagcaatcg gatttataaa attttggaac
1081 taaacgggta cgatccccaa tatgcggctt ccgtctttct gggatgggcc acgaaaaagt
1141 tcggcaagag gaacaccatc tggctgtttg ggcctgcaac taccgggaag accaacatcg
1201 cggaggccat agcccacact gtgcccttct acgggtgcgt aaactggacc aatgagaact
1261 ttcccttcaa cgactgtgtc gacaagatgg tgatctggtg ggaggagggg aagatgaccg
1321 ccaaggtcgt ggagtcggcc aaagccattc tcggaggaag caaggtgcgc gtggaccaga
1381 aatgcaagtc ctcggcccag atagacccga ctcccgtgat cgtcacctcc aacaccaaca
1441 tgtgcgccgt gattgacggg aactcaacga ccttcgaaca ccagcagccg ttgcaagacc
1501 ggatgttcaa atttgaactc acccgccgtc tggatcatga ctttgggaag gtcaccaagc
1561 aggaagtcaa agactttttc cggtgggcaa aggatcacgt ggttgaggtg gagcatgaat
1621 tctacgtcaa aaagggtgga gccaagaaaa gacccgcccc cagtgacgca gatataagtg
1681 agcccaaacg ggtgcgcgag tcagttgcgc agccatcgac gtcagacgcg gaagcttcga
1741 tcaactacgc agacaggtac caaaacaaat gttctcgtca cgtgggcatg aatctgatgc
1801 tgtttccctg cagacaatgc gagagaatga atcagaattc aaatatctgc ttcactcacg
1861 gacagaaaga ctgtttagag tgctttcccg tgtcagaatc tcaacccgtt tctgtcgtca
1921 aaaaggcgta tcagaaactg tgctacattc atcatatcat gggaaaggtg ccagacgctt
1981 gcactgcctg cgatctggtc aatgtggatt tggatgactg catctttgaa caataaatga
2041 tttaaatcag gtatggctgc cgatggttat cttccagatt ggctcgagga caaccttagt
2101 gaaggaattc gcgagtggtg ggctttgaaa cctggagccc ctcaacccaa ggcaaatcaa
2161 caacatcaag acaacgctcg aggtcttgtg cttccgggtt acaaatacct tggacccggc
2221 aacggactcg acaaggggga gccggtcaac gcagcagacg cggcggccct cgagcacgac
2281 aaggcctacg accagcagct caaggccgga gacaacccgt acctcaagta caaccacgcc
2341 gacgccgagt tccaggagcg gctcaaagaa gatacgtctt ttgggggcaa cctcgggcga
2401 gcagtcttcc aggccaaaaa gaggcttctt gaacctcttg gtctggttga ggaagcggct
2461 aagacggctc ctggaaagaa gaggcctgta gagcagtctc ctcaggaacc ggactcctcc
2521 gcgggtattg gcaaatcggg tgcacagccc gctaaaaaga gactcaattt cggtcagact
2581 ggcgacacag agtcagtccc agaccctcaa ccaatcggag aacctcccgc agccccctca
2641 ggtgtgggat ctcttacaat ggcttcaggt ggtggcgcac cagtggcaga caataacgaa
2701 ggtgccgatg gagtgggtag ttcctcggga aattggcatt gcgattccca atggctgggg
2761 gacagagtca tcaccaccag cacccgaacc tgggccctgc ccacctacaa caatcacctc
2821 tacaagcaaa tctccaacag cacatctgga ggatcttcaa atgacaacgc ctacttcggc
2881 tacagcaccc cctgggggta ttttgacttc aacagattcc actgccactt ctcaccacgt
2941 gactggcagc gactcatcaa caacaactgg ggattccggc ctaagcgact caacttcaag
3001 ctcttcaaca ttcaggtcaa agaggttacg gacaacaatg gagtcaagac catcgccaat
3061 aaccttacca gcacggtcca ggtcttcacg gactcagact atcagctccc gtacgtgctc
3121 gggtcggctc acgagggctg cctcccgccg ttcccagcgg acgttttcat gattcctcag
3181 tacgggtatc tgacgcttaa tgatggaagc caggccgtgg gtcgttcgtc cttttactgc
3241 ctggaatatt tcccgtcgca aatgctaaga acgggtaaca acttccagtt cagctacgag
3301 tttgagaacg tacctttcca tagcagctac gctcacagcc aaagcctgga ccgactaatg
3361 aatccactca tcgaccaata cttgtactat ctctctagaa ctattaacgg ttctggacag
3421 aatcaacaaa cgctaaaatt cagtgtggcc ggacccagca acatggctgt ccagggaaga
3481 aactacatac ctggacccag ctaccgacaa caacgtgtct caaccactgt gactcaaaac
3541 aacaacagcg aatttgcttg gcctggagct tcttcttggg ctctcaatgg acgtaatagc
3601 ttgatgaatc ctggacctgc tatggccagc cacaaagaag gagaggaccg tttctttcct
3661 ttgtctggat ctttaatttt tggcaaacaa ggtaccggta gagacaacgt ggatgcggac
3721 aaagtcatga taaccaacga agaagaaatt aaaactacta acccggtagc aacggagtcc
3781 tatggacaag tggccacaaa ccaccagagt gccactgagc ctccgaagac tacgtctgca
3841 caggcgcaga ccggttgggt tcaaaaccaa ggaatacttc cgggtatggt ttggcaggac
3901 agagatgtgt acctgcaagg acccatttgg gccaaaattc ctcacacgga cggcaacttt
3961 cacccttctc cgctgatggg agggtttgga atgaagcacc cgcctcctca gatcctcatc
4021 aaaaacacac ctgtacctgc ggatcctcca acggccttca acaaggacaa gctgaactct
4081 ttcatcaccc agtattctac tggccaagtc agcgtggaga tcgagtggga gctgcagaag
4141 gaaaacagca agcgctggaa cccggagatc cagtacactt ccaactatta caagtctaat
4201 aatgttgaat ttgctgttaa tactgaaggt gtatatagtg aaccccgccc cattggcacc
4261 agatacctga ctcgtaatct gtaagtttaa acctgcagga ctagaggtcc tgtattagag
4321 gtcacgtgag tgttttgcga cattttgcga caccatgtgg tcacgctggg tatttaagcc
4381 cgagtgagca cgcagggtct ccattttgaa gcgggaggtt tgaacgcgca gccgccaagc
4441 cgaattctgc agatatccat cacactggcg gccgctcgac tagagcggcc gccaccgcgg
4501 tggagctcca gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg
4561 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
4621 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
4681 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
4741 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
4801 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
4861 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
4921 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
4981 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
5041 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
5101 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
5161 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
5221 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
5281 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
5341 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
5401 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
5461 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
5521 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
5581 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
5641 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
5701 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
5761 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
5821 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
5881 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
5941 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
6001 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
6061 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
6121 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
6181 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
6241 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
6301 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
6361 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
6421 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
6481 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
6541 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
6601 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
6661 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa
6721 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
6781 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
6841 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
6901 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
6961 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
7021 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
7081 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
7141 cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc aactgttggg
7201 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
7261 caaggcgatt aagttgggta acg
//
