| 质粒名称: | pFUS_B7 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 31024 |
| 存储实验室: | Adam Bogdanove, Daniel Voytas |
|
载体骨架基本信息 |
|
| 载体名称: | pCR8 |
| 载体出品公司: | Invitrogen |
| 载体抗性: | 大观霉素 |
| 空载体大小: | 2447 bp |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | LacZ + BsaI restriction sites |
| 插入大小: | 459 bp |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | AflII (未破坏) |
| 3' 克隆位点: | XbaI(未破坏) |
| 5' 测序引物 | pCR8_F1 (ttgatgcctggcagttccct) |
| 3' 测序引物 | pCR8_R1 (cgaaccgaacaggcttatgt) |
|
文献引用方法 |
|
| 材料和方法部分: | pFUS_B7 was a gift from Adam Bogdanove & Daniel Voytas (Addgene plasmid # 31024 ; http://n2t.net/addgene:31024 ; RRID:Addgene_31024) |
| 参考文献部分: | Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller JA, Somia NV, Bogdanove AJ, Voytas DF. Nucleic Acids Res. 2011 Apr 14. ():. 10.1093/nar/gkr218 PubMed 21493687 |
买家导航
pFUSB7载体基本信息
pFUSB7载体图谱质粒图谱和多克隆位点信息
pFUSB7载体简介
pFUSB7载体序列
LOCUS pFUS_B7 3023 bp ds-DNA circular SYN 13-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pFUS_B7
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3023)
AUTHORS Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller
JA, Somia NV, Bogdanove AJ, Voytas DF
TITLE Efficient design and assembly of custom TALEN and other TAL
effector-based constructs for DNA targeting.
JOURNAL Nucleic Acids Res. 2011 Apr 14. ():.
PUBMED 21493687
REFERENCE 2 (bases 1 to 3023)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..3023
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 67..83
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 295..311
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 321..340
/label=T7
/note="T7 promoter, forward primer"
promoter 321..339
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(387..407)
/label=T3
/note="T3 promoter, forward primer"
promoter complement(387..405)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(426..442)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(426..442)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(439..461)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 450..466
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(474..504)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 519..540
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
CDS 1019..1810
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=SmR
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
rep_origin 1903..2491
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 2392..2411
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 2645..2662
/label=L4440
/note="L4440 vector, forward primer"
terminator 2821..2848
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator 2940..3
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
CDS complement(2947..430)
/codon_start=1
/gene="lacZ fragment"
/product="LacZ-alpha fragment of beta-galactosidase"
/label=lacZ-alpha
/translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGAGLDSH
HLVRGQAMRRLRLAVVLQRRDWENIHASVNARVGNCQASNKTKGSVGRLGLSFYLLFVG
ERSPE"
ORIGIN
1 ttgatgcctg gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac
61 gacgttgtaa aacgacggcc agtcttaagc gtctcatggc ctgaccccgg accaagtggt
121 ggctatcgag accggcgccg ctacagggcg cgtcccattc gccattcagg ctgcgcaact
181 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
241 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
301 cgacggccag tgagcgcgcg taatacgact cactataggg cgaattgggt accgggcccc
361 ccctcgaggt cctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa
421 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
481 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
541 attgcgttgc gctcactgcc cgctttccac cggtggtctc cgaaacggtg cagcggctgt
601 tgccggtgct gtgccaggac catggcctga ccccggacca agtggtggct atcgagacgt
661 ctagaccagc caggacagaa atgcctcgac ttcgctgcta cccaaggttg ccgggtgacg
721 cacaccgtgg aaacggatga aggcacgaac ccagtggaca taagcctgtt cggttcgtaa
781 gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag
841 cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttggggtaca
901 gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt
961 atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacattat
1021 gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga
1081 gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg
1141 cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac
1201 aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga
1261 gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta
1321 tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat
1381 cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca
1441 tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga
1501 tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg
1561 cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa
1621 aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca
1681 gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc
1741 gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt
1801 cggcaaataa ccctcgagcc acccatgacc aaaatccctt aacgtgagtt acgcgtcgtt
1861 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
1921 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
1981 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
2041 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
2101 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
2161 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
2221 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
2281 cctacagcgt gagcattgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
2341 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
2401 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
2461 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
2521 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
2581 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
2641 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
2701 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
2761 cagtgagcgc aacgcaatta atacgcgtac cgctagccag gaagagtttg tagaaacgca
2821 aaaaggccat ccgtcaggat ggccttctgc ttagtttgat gcctggcagt ttatggcggg
2881 cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga
2941 tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct
3001 tccgactgag cctttcgttt tat
//
