| 质粒名称: | pCG-Hc△18 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 84817 |
| 存储实验室: | Jakob Reiser |
|
载体骨架基本信息 |
|
| 载体名称: | pN1pCG |
| 载体出品公司: | Clontech |
| 空载体大小: | 4038 bp |
| 完整质粒大小: | 6403bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Measles virus H glycoprotein |
| 启动子: | CMV |
| 克隆方法: | 未知 |
| 5’测序引物 |
CTCCGCCCCATTGACGCA
(Common Sequencing Primers)
|
|
文献引用方法 |
|
| 材料和方法部分: |
pCG-Hc∆18 was a gift from Jakob Reiser (Addgene plasmid # 84817 ; http://n2t.net/addgene:84817 ; RRID:Addgene_84817)
|
| 参考文献部分: | A scalable method to concentrate lentiviral vectors pseudotyped with measles virus glycoproteins. Marino MP, Panigaj M, Ou W, Manirarora J, Wei CH, Reiser J. Gene Ther. 2015 Mar;22(3):280-5. doi: 10.1038/gt.2014.125. Epub 2015 Jan 22. 10.1038/gt.2014.125 PubMed 25608718 |
买家导航
pCG-Hc△18载体pCG-Hc18质粒基本信息
pCG-Hc△18质粒图谱pCG-Hc18载体图谱和pCG-Hc△18载体序列pCG-Hc18质粒序列多克隆位点信息
pCG-Hc△18质粒pCG-Hc18载体简介
pCG-Hc△18质粒序列pCG-Hc18载体序列
LOCUS pCG-Hc∆18 6403 bp DNA circular SYN 14-APR-2025
DEFINITION Measles virus hemagglutinin expressing plasmid.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6403)
AUTHORS Marino MP, Panigaj M, Ou W, Manirarora J, Wei CH, Reiser J
TITLE A scalable method to concentrate lentiviral vectors pseudotyped with
measles virus glycoproteins.
JOURNAL Gene Ther. 2015 Mar;22(3):280-5. doi: 10.1038/gt.2014.125. Epub 2015
Jan 22.
PUBMED 25608718
REFERENCE 2 (bases 1 to 6403)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1038/gt.2014.125"; journalName: "Gene Ther"; date: "2015-03";
volume: "22"; issue: "3"; pages: "280-5"
COMMENT Sequence Label: pCG-Hc∆18
FEATURES Location/Qualifiers
source 1..6403
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 17..320
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 321..524
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 475..495
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 521..545
/label=LNCX
/note="Human CMV promoter, forward primer"
intron 645..1218
/label=beta-globin intron
/note="intron from rabbit beta-globin gene"
primer_bind 1226..1245
/label=pCAG-F
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
promoter 1273..1291
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(3384..3403)
/label=Bglob-pA-R
/note="Rabbit beta-globin polyA region, reverse primer"
polyA_signal 3449..3504
/label=beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
primer_bind complement(3503..3522)
/label=rbglobpA-R
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
rep_origin 3858..3993
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind complement(4099..4116)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4270..4858)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4350..4369)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(5029..5820,5821..5889))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5652..5671
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(5890..5994)
/gene="bla"
/label=AmpR promoter
primer_bind 6062..6080
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(6118..6140)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 6240..6259
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
promoter 6378..6396
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
1 gaattcgagc tcgccccgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
61 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
121 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
181 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
241 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
301 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
361 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
421 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
481 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
541 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
601 ccagcctccg ggggatcgat ccccccgatc ctgagaactt cagggtgagt ttggggaccc
661 ttgattgttc tttctttttc gctattgtaa aattcatgtt atatggaggg ggcaaagttt
721 tcagggtgtt gtttagaatg ggaagatgtc ccttgtatca ccatggaccc tcatgataat
781 tttgtttctt tcactttcta ctctgttgac aaccattgtc tcctcttatt ttcttttcat
841 tttctgtaac tttttcgtta aactttagct tgcatttgta acgaattttt aaattcactt
901 ttgtttattt gtcagattgt aagtactttc tctaatcact ttttttttca aggcaatcag
961 ggtatattat attgtacttc agcacagttt tagagaacaa ttgttataat taaatgataa
1021 ggtagaatat ttctgcatat aaattctggc tggcgtggaa atattcttat tggtagaaac
1081 aactacatcc tggtcatcat cctgcctttc tctttatggt tacaatgata tacactgttt
1141 gagatgagga taaaatactc tgagtccaaa ccgggcccct ctgctaacca tgttcatgcc
1201 ttcttctttt tcctacagct cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt
1261 ggcaaagaat tgtaatacga ctcactatag ggcgaattcg gatcccccgg tagttaatta
1321 aaaattaggg tgcaagatca tcgataatgg gcagccggat cgtgatcaac cgggagcacc
1381 tgatgatcga ccggccctac gtgctgctgg ccgtgctgtt cgtgatgttc ctgagcctga
1441 tcggcttgct agccattgct ggaatccggc tgcacagagc cgccatctac accgccgaga
1501 tccacaagag cctgagcacc aacctggacg tgaccaacag catcgagcat caggtcaagg
1561 acgtgctgac ccccctgttt aagatcatcg gcgacgaagt gggcctgcgg accccccaga
1621 gattcaccga cctggtcaag ttcatcagcg acaagatcaa gttcctgaac cccgaccggg
1681 agtacgactt ccgggacctg acctggtgca tcaacccccc cgagcggatc aagctggact
1741 acgaccagta ctgcgccgat gtggccgccg aggaactgat gaatgcattg gtgaactcaa
1801 ctctactgga gaccagaaca accaatcagt tcctagctgt ctcaaaggga aactgctcag
1861 ggcccactac aatcagaggt caattctcaa acatgtcgct gtccctgtta gacttgtatt
1921 taggtcgagg ttacaatgtg tcatctatag tcactatgac atcccaggga atgtatgggg
1981 gaacttacct agtggaaaag cctaatctga gcagcaaaag gtcagagttg tcacaactga
2041 gcatgtaccg agtgtttgaa gtaggtgtta tcagaaatcc gggtttgggg gctccggtgt
2101 tccatatgac aaactatctt gagcaaccag tcagtaatga tctcagcaac tgtatggtgg
2161 ctttggggga gctcaaactc gcagcccttt gtcacgggga agattctatc acaattccct
2221 atcagggatc agggaaaggt gtcagcttcc agctcgtcaa gctaggtgtc tggaaatccc
2281 caaccgacat gcaatcctgg gtccccttat caacggatga tccagtgata gacaggcttt
2341 acctctcatc tcacagaggt gttatcgctg acaaccaagc aaaatgggct gtcccgacaa
2401 cacgaacaga tgacaagttg cgaatggaga catgcttcca acaggcgtgt aagggtaaaa
2461 tccaagcact ctgcgagaat cccgagtggg caccattgaa ggataacagg attccttcat
2521 acggggtctt gtctgttgat ctgagtctga cagttgagct taaaatcaaa attgcttcgg
2581 gattcgggcc attgatcaca cacggttcag ggatggacct atacaaatcc aaccacaaca
2641 atgtgtattg gctgactatc ccgccaatga agaacctagc cttaggtgta atcaacacat
2701 tggagtggat accgagattc aaggttagtc cctacctctt cactgtccca attaaggaag
2761 caggcggaga ctgccatgcc ccaacatacc tacctgcgga ggtggatggt gatgtcaaac
2821 tcagttccaa tctggtgatt ctacctggtc aagatctcca atatgttttg gcaacctacg
2881 atacttccag ggttgaacat gctgtggttt attacgttta cagcccaagc cgctcatttt
2941 cttactttta tccttttagg ttgcctataa agggggtccc catcgaatta caagtggaat
3001 gcttcacatg ggaccaaaaa ctctggtgcc gtcacttctg tgtgcttgcg gactcagaat
3061 ctggtggaca tatcactcac tctgggatgg tgggcatggg agtcagctgc acagtcaccc
3121 gggaagatgg aaccaatcgc agatagggct gctagtgaac caatcacatg atgtcaccca
3181 gacatcaggc atacccacta gtgtgaaata gacatcagaa ttaagaaaaa cgtagggtcc
3241 aagtggttcc ccgttatgga ctcgctatct gtcaaccaga tcttataccc tgaagttcac
3301 ctagatagcc cgatagttac caataagata gtagccatcc tggagtatgc tcgactgttt
3361 aaacctgcag gcatgcaagc tgatcttttt ccctctgcca aaaattatgg ggacatcatg
3421 aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg
3481 tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa
3541 catcagaatg agtatttggt ttagagtttg gcaacatatg cccatatgct ggctgccatg
3601 aacaaaggtt ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc
3661 ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta
3721 tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc
3781 ctctcctgac tactcccagt catagctgtc cctcttctct tatggagatc cctcgaggag
3841 ctttttgcaa aagcctaggc ctccaaaaaa gcctcttcac tacttctgga atagctcaga
3901 ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga
3961 atgggcggaa ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggactatggt
4021 tgctgactaa ttgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
4081 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
4141 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
4201 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
4261 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
4321 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
4381 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
4441 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
4501 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
4561 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
4621 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
4681 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
4741 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
4801 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
4861 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
4921 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
4981 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
5041 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
5101 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
5161 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
5221 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
5281 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
5341 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
5401 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
5461 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
5521 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
5581 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
5641 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
5701 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
5761 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
5821 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
5881 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
5941 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
6001 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
6061 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
6121 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
6181 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
6241 ggcatcagag cagattgtac tgagagtgca ccattcgacg ctctccctta tgcgactcct
6301 gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg
6361 gtctggctta tcgaaattaa tacgactcac tatagggaga ccg
//
pCG-Hc△18载体图谱pCG-Hc18质粒图谱pdf版和pCG-Hc△18载体序列pCG-Hc18质粒序列等相关资料下载
pCG-Hc△18质粒pCG-Hc18载体应用举例
- 上一篇：pET-28a_6H-MMLV_RT_D524N-6H
- 下一篇：pCfB6364
