pGL4.76[hRluc/Hygro]

价格:1500元

联系方式:I47-825O-882O

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pGL4.76载体基本信息

出品公司: Promega
载体名称: pGL4.76 ; pGL4.76[hRluc/Hygro]
质粒类型: 信号通路报告载体;哺乳动物载体;海肾荧光素酶报告载体
高拷贝/低拷贝: --
克隆方法: 限制性内切酶,多克隆位点
启动子:
载体大小: 5121 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: hRluc
载体抗性: 氨苄青霉素
筛选标记: 潮霉素(Hygromycin)
克隆菌株: TOP10等常规菌株
宿主细胞(系): HEK293等
备注: pGL4.76[hRluc/Hygro]载体是信号通路报告载体;无启动子;含海肾荧光素 酶报告基因hRluc。
产品目录号: E6941
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

pGL4.76载体质粒图谱和多克隆位点信息

pGL4.76载体图谱



pGL4.76 载体特征

pGL4.76载体简介

pGL4.76载体简介
The pGL4.76[hRluc/Hygro] Vector encodes the luciferase reporter gene hRluc (Renilla reniformis) and is designed for high expression and reduced anomalous transcription. This vector also contains a mammalian selectable marker for hygromycin resistance in which the number of transcription factor binding sites has been reduced and mammalian codon usage optimized. The pGL4 Vectors are engineered with fewer consensus regulatory sequences than the pGL3 Vectors and a synthetic reporter gene that has been codon optimized for mammalian expression. The pGL4.76[hRluc/Hygro] Vector is a basic vector with no promoter. However, it contains a multiple cloning region that allows cloning of a promoter of choice.

pGL4.76载体序列

LOCUS       AY864931                5121 bp    DNA     circular SYN 31-JAN-2005
DEFINITION  Luciferase reporter vector pGL4.76[hRluc/Hygro], complete sequence.
ACCESSION   AY864931
VERSION     AY864931.1  GI:58201877
KEYWORDS    .
SOURCE      Luciferase reporter vector pGL4.76[hRluc/Hygro]
  ORGANISM  Luciferase reporter vector pGL4.76[hRluc/Hygro]
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 5121)
  AUTHORS   Paguio,A., Almond,B. and Browning,J.
  TITLE     pGL4 Luciferase Reporter Vectors Technical Manual, TM259
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5121)
  AUTHORS   Paguio,A., Almond,B. and Browning,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-DEC-2004) Scientific Communications, Promega
            Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
FEATURES             Location/Qualifiers
     source          1..5121
                     /organism="Luciferase reporter vector
                     pGL4.76[hRluc/Hygro]"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:309057"
     misc_feature    1..70
                     /note="multiple cloning site"
     CDS             100..1035
                     /note="Renilla luciferase"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase hRluc"
                     /protein_id="AAW66991.1"
                     /db_xref="GI:58201878"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAE
                     NAVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLT
                     AWFELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIE
                     EDIALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLS
                     WPREIPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNT
                     EFVKVKGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     misc_feature    1067..1288
                     /note="SV40 late poly A"
     misc_feature    1336..1754
                     /note="SV40 enhancer/promoter"
     CDS             1779..2816
                     /codon_start=1
                     /transl_table=11
                     /product="synthetic hygromycin resistance protein"
                     /protein_id="AAW66992.1"
                     /db_xref="GI:58201879"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKEVGRV"
     regulatory      2840..2888
                     /regulatory_class="polyA_signal_sequence"
                     /note="synthetic polyadenylation signal sequence"
     primer_bind     2955..2974
                     /note="RVprimer4 binding site"
     rep_origin      3212
                     /note="ColEI-derived plasmid replication origin"
     CDS             complement(4003..4863)
                     /note="Ampr"
                     /codon_start=1
                     /transl_table=11
                     /product="synthetic beta-lactamase"
                     /protein_id="AAW66993.1"
                     /db_xref="GI:58201880"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     misc_feature    4968..5121
                     /note="synthetic poly(A) signal/transcriptional pause
                     site"
     primer_bind     5070..5089
                     /note="RVprimer3 binding site"
ORIGIN      
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
       61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggcttccaa ggtgtacgac
      121 cccgagcaac gcaaacgcat gatcactggg cctcagtggt gggctcgctg caagcaaatg
      181 aacgtgctgg actccttcat caactactat gattccgaga agcacgccga gaacgccgtg
      241 atttttctgc atggtaacgc tgcctccagc tacctgtgga ggcacgtcgt gcctcacatc
      301 gagcccgtgg ctagatgcat catccctgat ctgatcggaa tgggtaagtc cggcaagagc
      361 gggaatggct catatcgcct cctggatcac tacaagtacc tcaccgcttg gttcgagctg
      421 ctgaaccttc caaagaaaat catctttgtg ggccacgact ggggggcttg tctggccttt
      481 cactactcct acgagcacca agacaagatc aaggccatcg tccatgctga gagtgtcgtg
      541 gacgtgatcg agtcctggga cgagtggcct gacatcgagg aggatatcgc cctgatcaag
      601 agcgaagagg gcgagaaaat ggtgcttgag aataacttct tcgtcgagac catgctccca
      661 agcaagatca tgcggaaact ggagcctgag gagttcgctg cctacctgga gccattcaag
      721 gagaagggcg aggttagacg gcctaccctc tcctggcctc gcgagatccc tctcgttaag
      781 ggaggcaagc ccgacgtcgt ccagattgtc cgcaactaca acgcctacct tcgggccagc
      841 gacgatctgc ctaagatgtt catcgagtcc gaccctgggt tcttttccaa cgctattgtc
      901 gagggagcta agaagttccc taacaccgag ttcgtgaagg tgaagggcct ccacttcagc
      961 caggaggacg ctccagatga aatgggtaag tacatcaaga gcttcgtgga gcgcgtgctg
     1021 aagaacgagc agtaattcta gagtcggggc ggccggccgc ttcgagcaga catgataaga
     1081 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     1141 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     1201 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
     1261 agcaagtaaa acctctacaa atgtggtaaa atcgataagg atccgtttgc gtattgggcg
     1321 ctcttccgct gatctgcgca gcaccatggc ctgaaataac ctctgaaaga ggaacttggt
     1381 tagctacctt ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa
     1441 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     1501 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     1561 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1621 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1681 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1741 tttgcaaaaa gctcgattct tctgacacta gcgccaccat gaagaagccc gaactcaccg
     1801 ctaccagcgt tgaaaaattt ctcatcgaga agttcgacag tgtgagcgac ctgatgcagt
     1861 tgtcggaggg cgaagagagc cgagccttca gcttcgatgt cggcggacgc ggctatgtac
     1921 tgcgggtgaa tagctgcgct gatggcttct acaaagaccg ctacgtgtac cgccacttcg
     1981 ccagcgctgc actacccatc cccgaagtgt tggacatcgg cgagttcagc gagagcctga
     2041 catactgcat cagtagacgc gcccaaggcg ttactctcca agacctcccc gaaacagagc
     2101 tgcctgctgt gttacagcct gtcgccgaag ctatggatgc tattgccgcc gccgacctca
     2161 gtcaaaccag cggcttcggc ccattcgggc cccaaggcat cggccagtac acaacctggc
     2221 gggatttcat ttgcgccatt gctgatcccc atgtctacca ctggcagacc gtgatggacg
     2281 acaccgtgtc cgccagcgta gctcaagccc tggacgaact gatgctgtgg gccgaagact
     2341 gtcccgaggt gcgccacctc gtccatgccg acttcggcag caacaacgtc ctgaccgaca
     2401 acggccgcat caccgccgta atcgactggt ccgaagctat gttcggggac agtcagtacg
     2461 aggtggccaa catcttcttc tggcggccct ggctggcttg catggagcag cagactcgct
     2521 acttcgagcg ccggcatccc gagctggccg gcagccctcg tctgcgagcc tacatgctgc
     2581 gcatcggcct ggatcagctc taccagagcc tcgtggacgg caacttcgac gatgctgcct
     2641 gggctcaagg ccgctgcgat gccatcgtcc gcagcggggc cggcaccgtc ggtcgcacac
     2701 aaatcgctcg ccggagcgca gccgtatgga ccgacggctg cgtcgaggtg ctggccgaca
     2761 gcggcaaccg ccggcccagt acacgaccgc gcgctaagga ggtaggtcga gtttaaactc
     2821 tagaaccggt catggccgca ataaaatatc tttattttca ttacatctgt gtgttggttt
     2881 tttgtgtgtt cgaactagat gctgtcgacc gatgcccttg agagccttca acccagtcag
     2941 ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat
     3001 catgcaactc gtaggacagg tgccggcagc gctcttccgc ttcctcgctc actgactcgc
     3061 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     3121 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     3181 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     3241 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     3301 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     3361 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     3421 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     3481 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     3541 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     3601 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     3661 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     3721 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     3781 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     3841 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     3901 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     3961 cttggtctga cagcggccgc aaatgctaaa ccactgcagt ggttaccagt gcttgatcag
     4021 tgaggcaccg atctcagcga tctgcctatt tcgttcgtcc atagtggcct gactccccgt
     4081 cgtgtagatc actacgattc gtgagggctt accatcaggc cccagcgcag caatgatgcc
     4141 gcgagagccg cgttcaccgg cccccgattt gtcagcaatg aaccagccag cagggagggc
     4201 cgagcgaaga agtggtcctg ctactttgtc cgcctccatc cagtctatga gctgctgtcg
     4261 tgatgctaga gtaagaagtt cgccagtgag tagtttccga agagttgtgg ccattgctac
     4321 tggcatcgtg gtatcacgct cgtcgttcgg tatggcttcg ttcaactctg gttcccagcg
     4381 gtcaagccgg gtcacatgat cacccatatt atgaagaaat gcagtcagct ccttagggcc
     4441 tccgatcgtt gtcagaagta agttggccgc ggtgttgtcg ctcatggtaa tggcagcact
     4501 acacaattct cttaccgtca tgccatccgt aagatgcttt tccgtgaccg gcgagtactc
     4561 aaccaagtcg ttttgtgagt agtgtatacg gcgaccaagc tgctcttgcc cggcgtctat
     4621 acgggacaac accgcgccac atagcagtac tttgaaagtg ctcatcatcg ggaatcgttc
     4681 ttcggggcgg aaagactcaa ggatcttgcc gctattgaga tccagttcga tatagcccac
     4741 tcttgcaccc agttgatctt cagcatcttt tactttcacc agcgtttcgg ggtgtgcaaa
     4801 aacaggcaag caaaatgccg caaagaaggg aatgagtgcg acacgaaaat gttggatgct
     4861 catactcgtc ctttttcaat attattgaag catttatcag ggttactagt acgtctctca
     4921 aggataagta agtaatatta aggtacggga ggtattggac aggccgcaat aaaatatctt
     4981 tattttcatt acatctgtgt gttggttttt tgtgtgaatc gatagtacta acatacgctc
     5041 tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc agtgcaagtg
     5101 caggtgccag aacatttctc t
//

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